Restriction map for
pFN21AE0233
(Circular) (Six-base) MAPSORT of: pFN21AE0233.seq Check: 4736 from: 1 to: 5926
pFN21AE0233 5926 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3736 278
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2583 3269 1937
Size: 646 686 4594
Fragments arranged by size:
4594 686 646
Acc65I G'GTAC_C
Cuts at: 3247 3247
Size: 5926
AclI AA'CG_TT
Cuts at: 1563 2887 4053 4426 1563
Size: 1324 1166 373 3063
Fragments arranged by size:
3063 1324 1166 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2485 2552 2696 3989 5373 5641 1632
Size: 853 67 144 1293 1384 268 1917
Fragments arranged by size:
1917 1384 1293 853 268 144 67
AfeI AGC'GCT
Cuts at: 2144 3008 2144
Size: 864 5062
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5729
AflIII A'CryG_T
Cuts at: 1203 3045 4825 5802 1203
Size: 1842 1780 977 1327
Fragments arranged by size:
1842 1780 1327 977
AgeI A'CCGG_T
Cuts at: 4757 4757
Size: 5926
AhdI GACnn_n'nnGTC
Cuts at: 4655 4655
Size: 5926
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5926
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5926
AlwNI CAG_nnn'CTG
Cuts at: 2314 2344 3669 5241 2314
Size: 30 1325 1572 2999
Fragments arranged by size:
2999 1572 1325 30
ApaI G_GGCC'C
Cuts at: 2087 3114 2087
Size: 1027 4899
ApaLI G'TGCA_C
Cuts at: 3983 5139 3983
Size: 1156 4770
ApoI r'AATT_y
Cuts at: 1493 3235 3461 3613 5722 1493
Size: 1742 226 152 2109 1697
Fragments arranged by size:
2109 1742 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4480 160
Size: 872 3448 1606
Fragments arranged by size:
3448 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
AvaI C'yCGr_G
Cuts at: 1870 1943 2064 2174 2381 3251 1870
Size: 73 121 110 207 870 4545
Fragments arranged by size:
4545 870 207 121 110 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2647 2680 2647
Size: 33 5893
BamHI G'GATC_C
Cuts at: 3256 3623 3256
Size: 367 5559
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2170 2278 2323 3247 4702
Size: 325 217 627 383 108 45 924 1455
Cuts at: 4702 618
Size: 1842
Fragments arranged by size:
1842 1455 924 627 383 325 217 108
45
BanII G_rGCy'C
Cuts at: 1391 2087 3114 3245 1391
Size: 696 1027 131 4072
Fragments arranged by size:
4072 1027 696 131
BbeI G_GCGC'C
Cuts at: 2174 2282 2327 2174
Size: 108 45 5773
Fragments arranged by size:
5773 108 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4955
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4117 4151 1903
Size: 34 2180 34 3678
Fragments arranged by size:
3678 2180 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2675 3818 5028 2675
Size: 1143 1210 3573
Fragments arranged by size:
3573 1210 1143
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5926
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4537 136
Size: 107 122 71 631 605 2865 1525
Fragments arranged by size:
2865 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2250 2592 5921 2250
Size: 342 3329 2255
Fragments arranged by size:
3329 2255 342
BlpI GC'TnA_GC
Cuts at: 3343 3343
Size: 5926
Bme1580I G_kGCm'C
Cuts at: 1790 2087 3114 3987 5143 1790
Size: 297 1027 873 1156 2573
Fragments arranged by size:
2573 1156 1027 873 297
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5926
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2625 2776 2790
Size: 416 53 83 399 24 1208 151 14
Cuts at: 2790 3657 4615 442
Size: 867 958 1753
Fragments arranged by size:
1753 1208 958 867 416 399 151 83
53 24 14
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5926
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2707 2707
Size: 5926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 2131 2414 4586 1239
Size: 833 59 283 2172 2579
Fragments arranged by size:
2579 2172 833 283 59
Bpu10I CC'TnA_GC
Cuts at: 2698 3003 2698
Size: 305 5621
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2255 2275 2911 4051 4916 5214 2255
Size: 20 636 1140 865 298 2967
Fragments arranged by size:
2967 1140 865 636 298 20
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2417 4589 882
Size: 617 918 2172 2219
Fragments arranged by size:
2219 2172 918 617
BsaAI yAC'GTr
Cuts at: 493 1204 3717 493
Size: 711 2513 2702
Fragments arranged by size:
2702 2513 711
BsaBI GATnn'nnATC
Cuts at: 5920 5920
Size: 5926
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2171 2279 2324 2653
Size: 53 83 186 936 638 108 45 329
Cuts at: 2653 3733 4115 4750 275
Size: 1080 382 635 1451
Fragments arranged by size:
1451 1080 936 638 635 382 329 186
108 83 53 45
BsaWI w'CCGG_w
Cuts at: 4359 4757 5031 5178 5589 4359
Size: 398 274 147 411 4696
Fragments arranged by size:
4696 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2390 2420 3011 3041 1178
Size: 30 165 30 987 30 591 30 4063
Fragments arranged by size:
4063 987 591 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2411 1186
Size: 847 378 4701
Fragments arranged by size:
4701 847 378
BseYI C'CCAG_C
Cuts at: 2029 2455 2538 2572 2982 3107 5129 5625 2029
Size: 426 83 34 410 125 2022 496 2330
Fragments arranged by size:
2330 2022 496 426 410 125 83 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2672 3101 1163
Size: 573 936 429 3988
Fragments arranged by size:
3988 936 573 429
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2079 3376 4137 5165 1540
Size: 275 186 78 1297 761 1028 2301
Fragments arranged by size:
2301 1297 1028 761 275 186 78
BsiHKAI G_wGCw'C
Cuts at: 2013 2925 3987 4072 5143 2013
Size: 912 1062 85 1071 2796
Fragments arranged by size:
2796 1071 1062 912 85
BsmI GAATG_Cn'
Cuts at: 3437 3530 3437
Size: 93 5833
BsmBI CGTCTCn'nnnn_
Cuts at: 2441 2441
Size: 5926
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2087 2925 3114 3245 3987 4072
Size: 399 223 74 838 189 131 742 85
Cuts at: 4072 5143 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 838 742 399 223 189 131
85 74
BspEI T'CCGG_A
Cuts at: 5589 5589
Size: 5926
BspHI T'CATG_A
Cuts at: 3815 5545 3815
Size: 1730 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2595 3281 844
Size: 1751 686 3489
Fragments arranged by size:
3489 1751 686
BsrBI CCG'CTC
Cuts at: 1394 1823 2670 3813 1394
Size: 429 847 1143 3507
Fragments arranged by size:
3507 1143 847 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4421 4595 65
Size: 1183 3173 174 1396
Fragments arranged by size:
3173 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2151 2649 4570 4757 1700
Size: 110 341 498 1921 187 2869
Fragments arranged by size:
2869 1921 498 341 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5926
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5926
BssSI C'ACGA_G
Cuts at: 3986 4998 3986
Size: 1012 4914
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2314 2596 794
Size: 1520 282 4124
Fragments arranged by size:
4124 1520 282
BstBI TT'CG_AA
Cuts at: 4730 4730
Size: 5926
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5926
BstYI r'GATC_y
Cuts at: 1972 2250 2340 2592 3189 3256 3623 4008 4025
Size: 278 90 252 597 67 367 385 17
Cuts at: 4025 5477 5563 5575 5921 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 597 385 367 346 278 252
90 86 67 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2088 2166 513
Size: 552 939 84 78 4273
Fragments arranged by size:
4273 939 552 84 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2980 487
Size: 683 56 1754 3433
Fragments arranged by size:
3433 1754 683 56
BtsI GCAGTG_nn'
Cuts at: 3442 4236 4256 3442
Size: 794 20 5112
Fragments arranged by size:
5112 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5891
DraI TTT'AAA
Cuts at: 3230 3230
Size: 5926
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2813 4933 809
Size: 507 1497 2120 1802
Fragments arranged by size:
2120 1802 1497 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2076 2153 3031
Size: 54 1425 50 275 18 246 77 878
Cuts at: 3031 3373 4262 5891 8
Size: 342 889 1629 43
Fragments arranged by size:
1629 1425 889 878 342 275 246 77
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2076 3373 1537
Size: 275 264 1297 4090
Fragments arranged by size:
4090 1297 275 264
EarI CTCTTCn'nnn_
Cuts at: 1353 3856 1353
Size: 2503 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2658 2738 2911 4513 4887 5033
Size: 1064 138 1069 80 173 1602 374 146
Cuts at: 5033 387
Size: 1280
Fragments arranged by size:
1602 1280 1069 1064 374 173 146 138
80
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2131 2414 2485 2552 2696 3989
Size: 393 440 59 283 71 67 144 1293
Cuts at: 3989 4586 5373 5641 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1293 787 597 440 393 283 268
144 71 67 59
EcoNI CCTnn'n_nnAGG
Cuts at: 2396 2396
Size: 5926
EcoO109I rG'GnC_Cy
Cuts at: 2083 2084 3111 2083
Size: 1 1027 4898
Fragments arranged by size:
4898 1027 1
EcoRI G'AATT_C
Cuts at: 3235 3613 3235
Size: 378 5548
EcoRV GAT'ATC
Cuts at: 2951 2951
Size: 5926
FspI TGC'GCA
Cuts at: 4432 4432
Size: 5926
HaeII r_GCGC'y
Cuts at: 1678 2146 2174 2282 2327 3010 5073 1678
Size: 468 28 108 45 683 2063 2531
Fragments arranged by size:
2531 2063 683 468 108 45 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2909 2940 2941 2972 4567
Size: 32 135 32 1337 31 1 31 1595
Cuts at: 4567 4599 4641 4673 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1595 1337 135 42 32 32 32
32 31 31 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3270 3516 669
Size: 988 281 1332 246 3079
Fragments arranged by size:
3079 1332 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5926
HpaI GTT'AAC
Cuts at: 3516 3516
Size: 5926
KasI G'GCGC_C
Cuts at: 2170 2278 2323 2170
Size: 108 45 5773
Fragments arranged by size:
5773 108 45
KpnI G_GTAC'C
Cuts at: 3251 3251
Size: 5926
MfeI C'AATT_G
Cuts at: 3525 3525
Size: 5926
MluI A'CGCG_T
Cuts at: 5802 5802
Size: 5926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5040 5224 1305
Size: 3735 184 2007
Fragments arranged by size:
3735 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4104
Fragments arranged by size:
4104 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2435 3050 3131 3203 3884 4243 4402
Size: 648 1269 615 81 72 681 359 159
Cuts at: 4402 518
Size: 2042
Fragments arranged by size:
2042 1269 681 648 615 359 159 81
72
MspA1I CmG'CkG
Cuts at: 1606 2023 2090 2123 2311 2332 2542 2742 2940
Size: 417 67 33 188 21 210 200 198
Cuts at: 2940 3342 4019 5167 5412 1606
Size: 402 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 417 402 245 210 200
198 188 67 33 21
NaeI GCC'GGC
Cuts at: 2153 2651 2153
Size: 498 5428
NarI GG'CG_CC
Cuts at: 2171 2279 2324 2171
Size: 108 45 5773
Fragments arranged by size:
5773 108 45
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4435
Fragments arranged by size:
4435 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5926
NgoMIV G'CCGG_C
Cuts at: 2151 2649 2151
Size: 498 5428
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5926
NotI GC'GGCC_GC
Cuts at: 3373 3373
Size: 5926
NspI r_CATG'y
Cuts at: 1132 1229 3049 3151 3284 4829 1132
Size: 97 1820 102 133 1545 2229
Fragments arranged by size:
2229 1820 1545 133 102 97
PasI CC'CwG_GG
Cuts at: 1348 2824 1348
Size: 1476 4450
PciI A'CATG_T
Cuts at: 3045 4825 3045
Size: 1780 4146
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5926
PfoI T'CCnGG_A
Cuts at: 2343 2391 2343
Size: 48 5878
PmeI GTTT'AAAC
Cuts at: 3230 3230
Size: 5926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3032 3064 3995 4027 5508 5540 3032
Size: 32 931 32 1481 32 3418
Fragments arranged by size:
3418 1481 931 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5926
PsiI TTA'TAA
Cuts at: 3496 3496
Size: 5926
PspOMI G'GGCC_C
Cuts at: 2083 3110 2083
Size: 1027 4899
PstI C_TGCA'G
Cuts at: 830 2337 2379 2592 3278 830
Size: 1507 42 213 686 3478
Fragments arranged by size:
3478 1507 686 213 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5926
PvuII CAG'CTG
Cuts at: 2311 2542 2742 2311
Size: 231 200 5495
Fragments arranged by size:
5495 231 200
SacII CC_GC'GG
Cuts at: 2091 2091
Size: 5926
SalI G'TCGA_C
Cuts at: 1936 3268 1936
Size: 1332 4594
SbfI CC_TGCA'GG
Cuts at: 3278 3278
Size: 5926
ScaI AGT'ACT
Cuts at: 4174 5886 4174
Size: 1712 4214
SfcI C'TryA_G
Cuts at: 826 1045 2333 2375 2588 3274 4409 5090 5281
Size: 219 1288 42 213 686 1135 681 191
Cuts at: 5281 826
Size: 1471
Fragments arranged by size:
1471 1288 1135 686 681 219 213 191
42
SfoI GGC'GCC
Cuts at: 2172 2280 2325 2172
Size: 108 45 5773
Fragments arranged by size:
5773 108 45
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5926
SmaI CCC'GGG
Cuts at: 1872 2066 3253 1872
Size: 194 1187 4545
Fragments arranged by size:
4545 1187 194
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2254 2270 2926 4030 4931 5193
Size: 197 926 311 16 656 1104 901 262
Cuts at: 5193 820
Size: 1553
Fragments arranged by size:
1553 1104 926 901 656 311 262 197
16
SnaBI TAC'GTA
Cuts at: 493 3717 493
Size: 3224 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5926
SphI G_CATG'C
Cuts at: 1132 3284 1132
Size: 2152 3774
SspI AAT'ATT
Cuts at: 5 52 3850 5
Size: 47 3798 2081
Fragments arranged by size:
3798 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5926
StyI C'CwwG_G
Cuts at: 513 1065 2004 2319 2508 3365 513
Size: 552 939 315 189 857 3074
Fragments arranged by size:
3074 939 857 552 315 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2536 3953 3970 4123 4308 4753 325
Size: 1255 956 1417 17 153 185 445 1498
Fragments arranged by size:
1498 1417 1255 956 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2461 3042 4172
Size: 274 80 33 51 1443 484 581 1130
Cuts at: 4172 5884 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1130 581 484 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5886 5918 5886
Size: 32 5894
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5926
XbaI T'CTAG_A
Cuts at: 3262 3262
Size: 5926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2763 2763
Size: 5926
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5926
XmaI C'CCGG_G
Cuts at: 1870 2064 3251 1870
Size: 194 1187 4545
Fragments arranged by size:
4545 1187 194
XmnI GAAnn'nnTTC
Cuts at: 4055 4055
Size: 5926
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3734 276
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbvCI BfrBI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NruI
NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SrfI SwaI