Restriction map for
pFN21AE0221
(Circular) (Six-base) MAPSORT of: pFN21AE0221.seq Check: 1972 from: 1 to: 4993
pFN21AE0221 4993 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2803 278
Size: 53 83 186 936 1267 2468
Fragments arranged by size:
2468 1267 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2336 1937
Size: 399 4594
Acc65I G'GTAC_C
Cuts at: 2086 2314 2086
Size: 228 4765
AclI AA'CG_TT
Cuts at: 1563 3120 3493 1563
Size: 1557 373 3063
Fragments arranged by size:
3063 1557 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2222 3056 4440 4708 1632
Size: 590 834 1384 268 1917
Fragments arranged by size:
1917 1384 834 590 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4796
AflIII A'CryG_T
Cuts at: 1203 3892 4869 1203
Size: 2689 977 1327
Fragments arranged by size:
2689 1327 977
AgeI A'CCGG_T
Cuts at: 3824 3824
Size: 4993
AhdI GACnn_n'nnGTC
Cuts at: 3722 3722
Size: 4993
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4993
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2096 1184
Size: 912 4081
AlwNI CAG_nnn'CTG
Cuts at: 2736 4308 2736
Size: 1572 3421
ApaLI G'TGCA_C
Cuts at: 3050 4206 3050
Size: 1156 3837
ApoI r'AATT_y
Cuts at: 1493 2302 2528 2680 4789 1493
Size: 809 226 152 2109 1697
Fragments arranged by size:
2109 1697 809 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3547 160
Size: 872 2515 1606
Fragments arranged by size:
2515 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4993
AvaI C'yCGr_G
Cuts at: 1870 1943 2318 1870
Size: 73 375 4545
Fragments arranged by size:
4545 375 73
BamHI G'GATC_C
Cuts at: 2323 2690 2323
Size: 367 4626
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2086 2314 3769 618
Size: 325 217 627 299 228 1455 1842
Fragments arranged by size:
1842 1455 627 325 299 228 217
BanII G_rGCy'C
Cuts at: 1391 2312 1391
Size: 921 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4022
BbvCI CC'TCA_GC
Cuts at: 2267 2267
Size: 4993
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3184 3218 1903
Size: 34 50 34 1163 34 3678
Fragments arranged by size:
3678 1163 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2885 4095 2885
Size: 1210 3783
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4993
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3604 136
Size: 107 122 71 631 605 1932 1525
Fragments arranged by size:
1932 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4988 4988
Size: 4993
BlpI GC'TnA_GC
Cuts at: 2410 2410
Size: 4993
Bme1580I G_kGCm'C
Cuts at: 1790 3054 4210 1790
Size: 1264 1156 2573
Fragments arranged by size:
2573 1264 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4993
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2724 3682 442
Size: 416 53 83 399 24 1307 958 1753
Fragments arranged by size:
1753 1307 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2045 1056
Size: 989 4004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2080 3653 1239
Size: 841 1573 2579
Fragments arranged by size:
2579 1573 841
Bpu10I CC'TnA_GC
Cuts at: 2267 2267
Size: 4993
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3118 3983 4281 3118
Size: 865 298 3830
Fragments arranged by size:
3830 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3656 882
Size: 617 2157 2219
Fragments arranged by size:
2219 2157 617
BsaAI yAC'GTr
Cuts at: 493 1204 2784 493
Size: 711 1580 2702
Fragments arranged by size:
2702 1580 711
BsaBI GATnn'nnATC
Cuts at: 4987 4987
Size: 4993
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2800 3182 3817 275
Size: 53 83 186 936 1267 382 635 1451
Fragments arranged by size:
1451 1267 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3426 3824 4098 4245 4656 3426
Size: 398 274 147 411 3763
Fragments arranged by size:
3763 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4768
Fragments arranged by size:
4768 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4993
BseYI C'CCAG_C
Cuts at: 2045 4196 4692 2045
Size: 2151 496 2346
Fragments arranged by size:
2346 2151 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4420
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2443 3204 4232 1540
Size: 275 186 442 761 1028 2301
Fragments arranged by size:
2301 1028 761 442 275 186
BsiHKAI G_wGCw'C
Cuts at: 3054 3139 4210 3054
Size: 85 1071 3837
Fragments arranged by size:
3837 1071 85
BsmI GAATG_Cn'
Cuts at: 2504 2597 2504
Size: 93 4900
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2312 3054 3139 4210 1391
Size: 399 522 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 522 399 85
BspEI T'CCGG_A
Cuts at: 4656 4656
Size: 4993
BspHI T'CATG_A
Cuts at: 2882 4612 2882
Size: 1730 3263
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2074 2348 844
Size: 1230 274 3489
Fragments arranged by size:
3489 1230 274
BsrBI CCG'CTC
Cuts at: 1394 1823 2880 1394
Size: 429 1057 3507
Fragments arranged by size:
3507 1057 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3488 3662 65
Size: 1183 2240 174 1396
Fragments arranged by size:
2240 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3637 3824 1700
Size: 110 1827 187 2869
Fragments arranged by size:
2869 1827 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4993
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4993
BssSI C'ACGA_G
Cuts at: 3053 4065 3053
Size: 1012 3981
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4993
BstBI TT'CG_AA
Cuts at: 3797 3797
Size: 4993
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4993
BstXI CCAn_nnnn'nTGG
Cuts at: 2026 2026
Size: 4993
BstYI r'GATC_y
Cuts at: 1972 2323 2690 3075 3092 4544 4630 4642 4988
Size: 351 367 385 17 1452 86 12 346
Cuts at: 4988 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 351 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2135 2135
Size: 4993
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4441
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4254
Fragments arranged by size:
4254 683 56
BtsI GCAGTG_nn'
Cuts at: 2272 2509 3303 3323 2272
Size: 237 794 20 3942
Fragments arranged by size:
3942 794 237 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4993
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4958
DraI TTT'AAA
Cuts at: 2297 2297
Size: 4993
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4000 809
Size: 507 2684 1802
Fragments arranged by size:
2684 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2054 2440 3329
Size: 54 1425 50 275 18 224 386 889
Cuts at: 3329 4958 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 386 275 224 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2440 1537
Size: 275 628 4090
Fragments arranged by size:
4090 628 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2198 2923 1353
Size: 845 725 3423
Fragments arranged by size:
3423 845 725
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3580 3954 4100 387
Size: 1064 138 1991 374 146 1280
Fragments arranged by size:
1991 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2080 2222 3056 3653 4440 4708 1239
Size: 393 448 142 834 597 787 268 1524
Fragments arranged by size:
1524 834 787 597 448 393 268 142
EcoRI G'AATT_C
Cuts at: 2302 2680 2302
Size: 378 4615
FspI TGC'GCA
Cuts at: 3499 3499
Size: 4993
HaeII r_GCGC'y
Cuts at: 1678 4140 1678
Size: 2462 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3634 3666 3708 3740 1373
Size: 32 135 32 2062 32 42 32 2626
Fragments arranged by size:
2626 2062 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2337 2583 669
Size: 988 281 399 246 3079
Fragments arranged by size:
3079 988 399 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4993
HpaI GTT'AAC
Cuts at: 2583 2583
Size: 4993
KpnI G_GTAC'C
Cuts at: 2090 2318 2090
Size: 228 4765
MfeI C'AATT_G
Cuts at: 2592 2592
Size: 4993
MluI A'CGCG_T
Cuts at: 4869 4869
Size: 4993
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2218 4107 4291 1305
Size: 913 1889 184 2007
Fragments arranged by size:
2007 1889 913 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3171
Fragments arranged by size:
3171 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2951 3310 3469 518
Size: 648 1785 359 159 2042
Fragments arranged by size:
2042 1785 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2409 3086 4234 4479 1606
Size: 803 677 1148 245 2120
Fragments arranged by size:
2120 1148 803 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4441
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4993
NheI G'CTAG_C
Cuts at: 1052 2041 1052
Size: 989 4004
NotI GC'GGCC_GC
Cuts at: 2440 2440
Size: 4993
NspI r_CATG'y
Cuts at: 1132 1229 2083 2351 3896 1132
Size: 97 854 268 1545 2229
Fragments arranged by size:
2229 1545 854 268 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4993
PciI A'CATG_T
Cuts at: 3892 3892
Size: 4993
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4993
PmeI GTTT'AAAC
Cuts at: 2297 2297
Size: 4993
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3062 3094 4575 4607 3062
Size: 32 1481 32 3448
Fragments arranged by size:
3448 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4993
PsiI TTA'TAA
Cuts at: 2563 2563
Size: 4993
PstI C_TGCA'G
Cuts at: 830 2345 830
Size: 1515 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4993
SalI G'TCGA_C
Cuts at: 1936 2335 1936
Size: 399 4594
SbfI CC_TGCA'GG
Cuts at: 2345 2345
Size: 4993
ScaI AGT'ACT
Cuts at: 3241 4953 3241
Size: 1712 3281
SfcI C'TryA_G
Cuts at: 826 1045 2341 3476 4157 4348 826
Size: 219 1296 1135 681 191 1471
Fragments arranged by size:
1471 1296 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4993
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4993
SmaI CCC'GGG
Cuts at: 1872 2320 1872
Size: 448 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3097 3998 4260 820
Size: 197 926 1154 901 262 1553
Fragments arranged by size:
1553 1154 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2784 493
Size: 2291 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4993
SphI G_CATG'C
Cuts at: 1132 2083 2351 1132
Size: 951 268 3774
Fragments arranged by size:
3774 951 268
SspI AAT'ATT
Cuts at: 5 52 2193 2917 5
Size: 47 2141 724 2081
Fragments arranged by size:
2141 2081 724 47
StuI AGG'CCT
Cuts at: 1517 2219 1517
Size: 702 4291
StyI C'CwwG_G
Cuts at: 513 1065 2226 2432 513
Size: 552 1161 206 3074
Fragments arranged by size:
3074 1161 552 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3020 3037 3190 3375 3820 325
Size: 1255 1440 17 153 185 445 1498
Fragments arranged by size:
1498 1440 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3239 4951 96
Size: 274 80 33 51 1443 1262 1712 138
Fragments arranged by size:
1712 1443 1262 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4953 4985 4953
Size: 32 4961
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4993
XbaI T'CTAG_A
Cuts at: 2329 2329
Size: 4993
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4993
XmaI C'CCGG_G
Cuts at: 1870 2318 1870
Size: 448 4545
XmnI GAAnn'nnTTC
Cuts at: 3122 3122
Size: 4993
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2801 276
Size: 53 83 186 936 1267 2468
Fragments arranged by size:
2468 1267 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI