Restriction map for
pFN21AE0217
(Circular) (Six-base) MAPSORT of: pFN21AE0217.seq Check: 9675 from: 1 to: 5221
pFN21AE0217 5221 bp
With 182 enzymes: SgfI *
October 20, 2009 10:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3031 278
Size: 53 83 186 936 1495 2468
Fragments arranged by size:
2468 1495 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2184 2564 1937
Size: 247 380 4594
Fragments arranged by size:
4594 380 247
Acc65I G'GTAC_C
Cuts at: 2542 2542
Size: 5221
AclI AA'CG_TT
Cuts at: 1563 2462 3348 3721 1563
Size: 899 886 373 3063
Fragments arranged by size:
3063 899 886 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3284 4668 4936 1632
Size: 1652 1384 268 1917
Fragments arranged by size:
1917 1652 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5024
AflIII A'CryG_T
Cuts at: 1203 2094 4120 5097 1203
Size: 891 2026 977 1327
Fragments arranged by size:
2026 1327 977 891
AgeI A'CCGG_T
Cuts at: 4052 4052
Size: 5221
AhdI GACnn_n'nnGTC
Cuts at: 3950 3950
Size: 5221
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5221
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5221
AlwNI CAG_nnn'CTG
Cuts at: 2964 4536 2964
Size: 1572 3649
ApaLI G'TGCA_C
Cuts at: 3278 4434 3278
Size: 1156 4065
ApoI r'AATT_y
Cuts at: 1493 2299 2385 2530 2756 2908 5017 1493
Size: 806 86 145 226 152 2109 1697
Fragments arranged by size:
2109 1697 806 226 152 145 86
AseI AT'TA_AT
Cuts at: 160 1032 3775 160
Size: 872 2743 1606
Fragments arranged by size:
2743 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5221
AvaI C'yCGr_G
Cuts at: 1870 1943 2546 1870
Size: 73 603 4545
Fragments arranged by size:
4545 603 73
BamHI G'GATC_C
Cuts at: 2551 2918 2551
Size: 367 4854
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2542 3997 618
Size: 325 217 627 755 1455 1842
Fragments arranged by size:
1842 1455 755 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2078 2540 1391
Size: 687 462 4072
Fragments arranged by size:
4072 687 462
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2241 928
Size: 971 342 3908
Fragments arranged by size:
3908 971 342
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2012 2046 3412 3446 1903
Size: 34 75 34 1366 34 3678
Fragments arranged by size:
3678 1366 75 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3113 4323 3113
Size: 1210 4011
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5221
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2252 3832 136
Size: 107 122 71 631 605 580 1580 1525
Fragments arranged by size:
1580 1525 631 605 580 122 107 71
BglII A'GATC_T
Cuts at: 5216 5216
Size: 5221
BlpI GC'TnA_GC
Cuts at: 2638 2638
Size: 5221
Bme1580I G_kGCm'C
Cuts at: 1790 2133 3282 4438 1790
Size: 343 1149 1156 2573
Fragments arranged by size:
2573 1156 1149 343
BmgBI CAC'GTC
Cuts at: 1318 2095 1318
Size: 777 4444
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2952 3910 442
Size: 416 53 83 399 24 1535 958 1753
Fragments arranged by size:
1753 1535 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2064 2486 3881 1239
Size: 825 422 1395 2579
Fragments arranged by size:
2579 1395 825 422
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3346 4211 4509 3346
Size: 865 298 4058
Fragments arranged by size:
4058 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2143 2490 3884 882
Size: 617 644 347 1394 2219
Fragments arranged by size:
2219 1394 644 617 347
BsaAI yAC'GTr
Cuts at: 493 1204 3012 493
Size: 711 1808 2702
Fragments arranged by size:
2702 1808 711
BsaBI GATnn'nnATC
Cuts at: 2177 5215 2177
Size: 3038 2183
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3028 3410 4045 275
Size: 53 83 186 936 1495 382 635 1451
Fragments arranged by size:
1495 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3654 4052 4326 4473 4884 3654
Size: 398 274 147 411 3991
Fragments arranged by size:
3991 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4996
Fragments arranged by size:
4996 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5221
BseYI C'CCAG_C
Cuts at: 4424 4920 4424
Size: 496 4725
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4648
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2671 3432 4460 1540
Size: 275 186 670 761 1028 2301
Fragments arranged by size:
2301 1028 761 670 275 186
BsiHKAI G_wGCw'C
Cuts at: 2078 2400 3282 3367 4438 2078
Size: 322 882 85 1071 2861
Fragments arranged by size:
2861 1071 882 322 85
BsmI GAATG_Cn'
Cuts at: 2454 2732 2825 2454
Size: 278 93 4850
Fragments arranged by size:
4850 278 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2133 2400 2540 3282 3367 4438
Size: 399 288 55 267 140 742 85 1071
Cuts at: 4438 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 288 267 140 85
55
BspEI T'CCGG_A
Cuts at: 4884 4884
Size: 5221
BspHI T'CATG_A
Cuts at: 3110 4840 3110
Size: 1730 3491
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2576 844
Size: 1732 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3108 1394
Size: 429 1285 3507
Fragments arranged by size:
3507 1285 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3716 3890 65
Size: 1183 2468 174 1396
Fragments arranged by size:
2468 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3865 4052 1700
Size: 110 2055 187 2869
Fragments arranged by size:
2869 2055 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5221
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5221
BssSI C'ACGA_G
Cuts at: 3281 4293 3281
Size: 1012 4209
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5221
BstBI TT'CG_AA
Cuts at: 4025 4025
Size: 5221
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5221
BstYI r'GATC_y
Cuts at: 1972 2551 2918 3303 3320 4772 4858 4870 5216
Size: 579 367 385 17 1452 86 12 346
Cuts at: 5216 1972
Size: 1977
Fragments arranged by size:
1977 1452 579 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2034 513
Size: 552 939 30 3700
Fragments arranged by size:
3700 939 552 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2043 2435 487
Size: 683 56 817 392 3273
Fragments arranged by size:
3273 817 683 392 56
BtsI GCAGTG_nn'
Cuts at: 2737 3531 3551 2737
Size: 794 20 4407
Fragments arranged by size:
4407 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5221
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5186
DraI TTT'AAA
Cuts at: 2061 2525 2061
Size: 464 4757
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4228 809
Size: 507 2912 1802
Fragments arranged by size:
2912 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2053 2668
Size: 54 1425 50 275 18 189 34 615
Cuts at: 2668 3557 5186 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 615 275 189 54 50
43 34 18
EagI C'GGCC_G
Cuts at: 1537 1812 2668 1537
Size: 275 856 4090
Fragments arranged by size:
4090 856 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3151 1353
Size: 1798 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3808 4182 4328 387
Size: 1064 138 2219 374 146 1280
Fragments arranged by size:
2219 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2076 2076
Size: 5221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2064 2486 3284 3881 4668 4936 1239
Size: 393 432 422 798 597 787 268 1524
Fragments arranged by size:
1524 798 787 597 432 422 393 268
EcoRI G'AATT_C
Cuts at: 2530 2908 2530
Size: 378 4843
FspI TGC'GCA
Cuts at: 3727 3727
Size: 5221
HaeII r_GCGC'y
Cuts at: 1678 4368 1678
Size: 2690 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3862 3894 3936 3968 1373
Size: 32 135 32 2290 32 42 32 2626
Fragments arranged by size:
2626 2290 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2565 2811 669
Size: 988 281 627 246 3079
Fragments arranged by size:
3079 988 627 281 246
HindIII A'AGCT_T
Cuts at: 748 2513 748
Size: 1765 3456
HpaI GTT'AAC
Cuts at: 2811 2811
Size: 5221
KpnI G_GTAC'C
Cuts at: 2546 2546
Size: 5221
MfeI C'AATT_G
Cuts at: 2472 2820 2472
Size: 348 4873
MluI A'CGCG_T
Cuts at: 5097 5097
Size: 5221
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4335 4519 1305
Size: 3030 184 2007
Fragments arranged by size:
3030 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2055 10
Size: 54 1425 343 223 3176
Fragments arranged by size:
3176 1425 343 223 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3179 3538 3697 518
Size: 648 2013 359 159 2042
Fragments arranged by size:
2042 2013 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2637 3314 4462 4707 1606
Size: 1031 677 1148 245 2120
Fragments arranged by size:
2120 1148 1031 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3730
Fragments arranged by size:
3730 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5221
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5221
NotI GC'GGCC_GC
Cuts at: 2668 2668
Size: 5221
NspI r_CATG'y
Cuts at: 1132 1229 2579 4124 1132
Size: 97 1350 1545 2229
Fragments arranged by size:
2229 1545 1350 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5221
PciI A'CATG_T
Cuts at: 4120 4120
Size: 5221
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2126 1108
Size: 1018 4203
PmeI GTTT'AAAC
Cuts at: 2525 2525
Size: 5221
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3290 3322 4803 4835 3290
Size: 32 1481 32 3676
Fragments arranged by size:
3676 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5221
PsiI TTA'TAA
Cuts at: 2791 2791
Size: 5221
PstI C_TGCA'G
Cuts at: 830 2573 830
Size: 1743 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5221
SacI G_AGCT'C
Cuts at: 2078 2078
Size: 5221
SalI G'TCGA_C
Cuts at: 1936 2563 1936
Size: 627 4594
SbfI CC_TGCA'GG
Cuts at: 2573 2573
Size: 5221
ScaI AGT'ACT
Cuts at: 3469 5181 3469
Size: 1712 3509
SfcI C'TryA_G
Cuts at: 826 1045 2569 3704 4385 4576 826
Size: 219 1524 1135 681 191 1471
Fragments arranged by size:
1524 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5221
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5221
SmaI CCC'GGG
Cuts at: 1872 2548 1872
Size: 676 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3325 4226 4488 820
Size: 197 926 1382 901 262 1553
Fragments arranged by size:
1553 1382 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3012 493
Size: 2519 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5221
SphI G_CATG'C
Cuts at: 1132 2579 1132
Size: 1447 3774
SspI AAT'ATT
Cuts at: 5 52 3145 5
Size: 47 3093 2081
Fragments arranged by size:
3093 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5221
StyI C'CwwG_G
Cuts at: 513 1065 2004 2660 513
Size: 552 939 656 3074
Fragments arranged by size:
3074 939 656 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2415 3248 3265 3418 3603 4048 325
Size: 1255 835 833 17 153 185 445 1498
Fragments arranged by size:
1498 1255 835 833 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2097 3467 5179
Size: 274 80 33 51 1443 120 1370 1712
Cuts at: 5179 96
Size: 138
Fragments arranged by size:
1712 1443 1370 274 138 120 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5181 5213 5181
Size: 32 5189
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5221
XbaI T'CTAG_A
Cuts at: 2557 2557
Size: 5221
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5221
XmaI C'CCGG_G
Cuts at: 1870 2546 1870
Size: 676 4545
XmnI GAAnn'nnTTC
Cuts at: 3350 3350
Size: 5221
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3029 276
Size: 53 83 186 936 1495 2468
Fragments arranged by size:
2468 1495 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI