Restriction map for
pFN21AE0205
(Circular) (Six-base) MAPSORT of: pFN21AE0205.seq Check: 321 from: 1 to: 5347
pFN21AE0205 5347 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3157 278
Size: 53 83 186 936 1621 2468
Fragments arranged by size:
2468 1621 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2690 1937
Size: 753 4594
Acc65I G'GTAC_C
Cuts at: 2668 2668
Size: 5347
AclI AA'CG_TT
Cuts at: 1563 3474 3847 1563
Size: 1911 373 3063
Fragments arranged by size:
3063 1911 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2211 2414 3410 4794 5062 1632
Size: 579 203 996 1384 268 1917
Fragments arranged by size:
1917 1384 996 579 268 203
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5150
AflIII A'CryG_T
Cuts at: 1203 4246 5223 1203
Size: 3043 977 1327
Fragments arranged by size:
3043 1327 977
AgeI A'CCGG_T
Cuts at: 2031 4178 2031
Size: 2147 3200
AhdI GACnn_n'nnGTC
Cuts at: 4076 4076
Size: 5347
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5347
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5347
AlwNI CAG_nnn'CTG
Cuts at: 3090 4662 3090
Size: 1572 3775
ApaLI G'TGCA_C
Cuts at: 3404 4560 3404
Size: 1156 4191
ApoI r'AATT_y
Cuts at: 1493 2543 2656 2882 3034 5143 1493
Size: 1050 113 226 152 2109 1697
Fragments arranged by size:
2109 1697 1050 226 152 113
AseI AT'TA_AT
Cuts at: 160 1032 3901 160
Size: 872 2869 1606
Fragments arranged by size:
2869 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5347
AvaI C'yCGr_G
Cuts at: 1870 1943 2385 2672 1870
Size: 73 442 287 4545
Fragments arranged by size:
4545 442 287 73
BamHI G'GATC_C
Cuts at: 2677 3044 2677
Size: 367 4980
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2010 2354 2668 4123 618
Size: 325 217 627 223 344 314 1455 1842
Fragments arranged by size:
1842 1455 627 344 325 314 223 217
BanII G_rGCy'C
Cuts at: 1391 2666 1391
Size: 1275 4072
BbeI G_GCGC'C
Cuts at: 2014 2014
Size: 5347
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2178 2556 928
Size: 971 279 378 3719
Fragments arranged by size:
3719 971 378 279
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3538 3572 1903
Size: 34 1601 34 3678
Fragments arranged by size:
3678 1601 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3239 4449 3239
Size: 1210 4137
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5347
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2201 3958 136
Size: 107 122 71 631 605 529 1757 1525
Fragments arranged by size:
1757 1525 631 605 529 122 107 71
BglII A'GATC_T
Cuts at: 5342 5342
Size: 5347
BlpI GC'TnA_GC
Cuts at: 2764 2764
Size: 5347
Bme1580I G_kGCm'C
Cuts at: 1790 3408 4564 1790
Size: 1618 1156 2573
Fragments arranged by size:
2573 1618 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5347
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2601 3078 4036
Size: 416 53 83 399 24 1184 477 958
Cuts at: 4036 442
Size: 1753
Fragments arranged by size:
1753 1184 958 477 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5347
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2291 2291
Size: 5347
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2321 4007 1239
Size: 980 102 1686 2579
Fragments arranged by size:
2579 1686 980 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3472 4337 4635 3472
Size: 865 298 4184
Fragments arranged by size:
4184 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4010 882
Size: 617 2511 2219
Fragments arranged by size:
2511 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3138 493
Size: 711 1934 2702
Fragments arranged by size:
2702 1934 711
BsaBI GATnn'nnATC
Cuts at: 2099 2501 5341 2099
Size: 402 2840 2105
Fragments arranged by size:
2840 2105 402
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2011 2488 3154 3536
Size: 53 83 186 936 478 477 666 382
Cuts at: 3536 4171 275
Size: 635 1451
Fragments arranged by size:
1451 936 666 635 478 477 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2031 3780 4178 4452 4599 5010 2031
Size: 1749 398 274 147 411 2368
Fragments arranged by size:
2368 1749 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5122
Fragments arranged by size:
5122 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2453 1186
Size: 1267 4080
BseYI C'CCAG_C
Cuts at: 2321 4550 5046 2321
Size: 2229 496 2622
Fragments arranged by size:
2622 2229 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2624 1163
Size: 573 888 3886
Fragments arranged by size:
3886 888 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2797 3558 4586 1540
Size: 275 186 796 761 1028 2301
Fragments arranged by size:
2301 1028 796 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3408 3493 4564 3408
Size: 85 1071 4191
Fragments arranged by size:
4191 1071 85
BsmI GAATG_Cn'
Cuts at: 2858 2951 2858
Size: 93 5254
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2666 3408 3493 4564 1391
Size: 399 876 742 85 1071 2174
Fragments arranged by size:
2174 1071 876 742 399 85
BspEI T'CCGG_A
Cuts at: 5010 5010
Size: 5347
BspHI T'CATG_A
Cuts at: 3236 4966 3236
Size: 1730 3617
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2702 844
Size: 1858 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3234 1394
Size: 429 1411 3507
Fragments arranged by size:
3507 1411 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3842 4016 65
Size: 1183 2594 174 1396
Fragments arranged by size:
2594 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2031 3991 4178 1700
Size: 110 221 1960 187 2869
Fragments arranged by size:
2869 1960 221 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5347
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5347
BssSI C'ACGA_G
Cuts at: 3407 4419 3407
Size: 1012 4335
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5347
BstBI TT'CG_AA
Cuts at: 4151 4151
Size: 5347
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5347
BstYI r'GATC_y
Cuts at: 1972 2094 2677 3044 3429 3446 4898 4984 4996
Size: 122 583 367 385 17 1452 86 12
Cuts at: 4996 5342 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 583 385 367 346 122 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3856
Fragments arranged by size:
3856 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4608
Fragments arranged by size:
4608 683 56
BtsI GCAGTG_nn'
Cuts at: 2863 3657 3677 2863
Size: 794 20 4533
Fragments arranged by size:
4533 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5347
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5312
DraI TTT'AAA
Cuts at: 2651 2651
Size: 5347
DraIII CAC_nnn'GTG
Cuts at: 2150 2161 2150
Size: 11 5336
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4354 809
Size: 507 3038 1802
Fragments arranged by size:
3038 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2152 2559 2794
Size: 54 1425 50 275 18 322 407 235
Cuts at: 2794 3683 5312 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 407 322 275 235 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2794 1537
Size: 275 982 4090
Fragments arranged by size:
4090 982 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3277 1353
Size: 1924 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3934 4308 4454 387
Size: 1064 138 2345 374 146 1280
Fragments arranged by size:
2345 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2211 2219 2321 2414 3410 4007 4794
Size: 393 579 8 102 93 996 597 787
Cuts at: 4794 5062 1239
Size: 268 1524
Fragments arranged by size:
1524 996 787 597 579 393 268 102
93 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2461 2461
Size: 5347
EcoRI G'AATT_C
Cuts at: 2656 3034 2656
Size: 378 4969
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2536 2536
Size: 5347
FspI TGC'GCA
Cuts at: 2462 3853 2462
Size: 1391 3956
HaeII r_GCGC'y
Cuts at: 1678 2014 4494 1678
Size: 336 2480 2531
Fragments arranged by size:
2531 2480 336
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2168 2200 3988 4020 4062
Size: 32 135 32 596 32 1788 32 42
Cuts at: 4062 4094 1373
Size: 32 2626
Fragments arranged by size:
2626 1788 596 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2691 2937 669
Size: 988 281 753 246 3079
Fragments arranged by size:
3079 988 753 281 246
HindIII A'AGCT_T
Cuts at: 748 2426 748
Size: 1678 3669
HpaI GTT'AAC
Cuts at: 2937 2937
Size: 5347
KasI G'GCGC_C
Cuts at: 2010 2010
Size: 5347
KpnI G_GTAC'C
Cuts at: 2672 2672
Size: 5347
MfeI C'AATT_G
Cuts at: 2946 2946
Size: 5347
MluI A'CGCG_T
Cuts at: 5223 5223
Size: 5347
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4461 4645 1305
Size: 3156 184 2007
Fragments arranged by size:
3156 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2154 2561 10
Size: 54 1425 343 322 407 2796
Fragments arranged by size:
2796 1425 407 343 322 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2267 3305 3664 3823 518
Size: 648 1101 1038 359 159 2042
Fragments arranged by size:
2042 1101 1038 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2182 2763 3440 4588 4833 1606
Size: 576 581 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 581 576 245
NarI GG'CG_CC
Cuts at: 2011 2011
Size: 5347
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3856
Fragments arranged by size:
3856 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5347
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5347
NotI GC'GGCC_GC
Cuts at: 2794 2794
Size: 5347
NspI r_CATG'y
Cuts at: 1132 1229 2266 2447 2705 4250 1132
Size: 97 1037 181 258 1545 2229
Fragments arranged by size:
2229 1545 1037 258 181 97
PasI CC'CwG_GG
Cuts at: 1348 2371 1348
Size: 1023 4324
PciI A'CATG_T
Cuts at: 4246 4246
Size: 5347
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2150 2514 1108
Size: 1042 364 3941
Fragments arranged by size:
3941 1042 364
PmeI GTTT'AAAC
Cuts at: 2651 2651
Size: 5347
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3416 3448 4929 4961 3416
Size: 32 1481 32 3802
Fragments arranged by size:
3802 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5347
PsiI TTA'TAA
Cuts at: 2917 2917
Size: 5347
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2036 2068 2036
Size: 32 5315
PstI C_TGCA'G
Cuts at: 830 2699 830
Size: 1869 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5347
PvuII CAG'CTG
Cuts at: 2182 2182
Size: 5347
SalI G'TCGA_C
Cuts at: 1936 2689 1936
Size: 753 4594
SbfI CC_TGCA'GG
Cuts at: 2699 2699
Size: 5347
ScaI AGT'ACT
Cuts at: 3595 5307 3595
Size: 1712 3635
SfcI C'TryA_G
Cuts at: 826 1045 2379 2695 3830 4511 4702 826
Size: 219 1334 316 1135 681 191 1471
Fragments arranged by size:
1471 1334 1135 681 316 219 191
SfoI GGC'GCC
Cuts at: 2012 2012
Size: 5347
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5347
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5347
SmaI CCC'GGG
Cuts at: 1872 2387 2674 1872
Size: 515 287 4545
Fragments arranged by size:
4545 515 287
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3451 4352 4614 820
Size: 197 926 1508 901 262 1553
Fragments arranged by size:
1553 1508 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3138 493
Size: 2645 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5347
SphI G_CATG'C
Cuts at: 1132 2705 1132
Size: 1573 3774
SspI AAT'ATT
Cuts at: 5 52 3271 5
Size: 47 3219 2081
Fragments arranged by size:
3219 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5347
StyI C'CwwG_G
Cuts at: 513 1065 2004 2619 2786 513
Size: 552 939 615 167 3074
Fragments arranged by size:
3074 939 615 552 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3374 3391 3544 3729 4174 325
Size: 1255 1794 17 153 185 445 1498
Fragments arranged by size:
1794 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3593 5305 96
Size: 274 80 33 51 1443 1616 1712 138
Fragments arranged by size:
1712 1616 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5307 5339 5307
Size: 32 5315
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5347
XbaI T'CTAG_A
Cuts at: 2683 2683
Size: 5347
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2227 2227
Size: 5347
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5347
XmaI C'CCGG_G
Cuts at: 1870 2385 2672 1870
Size: 515 287 4545
Fragments arranged by size:
4545 515 287
XmnI GAAnn'nnTTC
Cuts at: 3476 3476
Size: 5347
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3155 276
Size: 53 83 186 936 1621 2468
Fragments arranged by size:
2468 1621 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI