Restriction map for
pFN21AE0204
(Circular) (Six-base) MAPSORT of: pFN21AE0204.seq Check: 9073 from: 1 to: 5326
pFN21AE0204 5326 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3136 278
Size: 53 83 186 936 1600 2468
Fragments arranged by size:
2468 1600 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2669 1937
Size: 732 4594
Acc65I G'GTAC_C
Cuts at: 2647 2647
Size: 5326
AclI AA'CG_TT
Cuts at: 1563 3453 3826 1563
Size: 1890 373 3063
Fragments arranged by size:
3063 1890 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2543 3389 4773 5041 1632
Size: 911 846 1384 268 1917
Fragments arranged by size:
1917 1384 911 846 268
AfeI AGC'GCT
Cuts at: 2534 2534
Size: 5326
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5129
AflIII A'CryG_T
Cuts at: 1203 4225 5202 1203
Size: 3022 977 1327
Fragments arranged by size:
3022 1327 977
AgeI A'CCGG_T
Cuts at: 4157 4157
Size: 5326
AhdI GACnn_n'nnGTC
Cuts at: 4055 4055
Size: 5326
AleI CACnn'nnGTG
Cuts at: 1166 2582 1166
Size: 1416 3910
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5326
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2372 2404 2372
Size: 32 5294
AlwNI CAG_nnn'CTG
Cuts at: 2077 2209 3069 4641 2077
Size: 132 860 1572 2762
Fragments arranged by size:
2762 1572 860 132
ApaI G_GGCC'C
Cuts at: 2492 2492
Size: 5326
ApaLI G'TGCA_C
Cuts at: 3383 4539 3383
Size: 1156 4170
ApoI r'AATT_y
Cuts at: 1493 2318 2635 2861 3013 5122 1493
Size: 825 317 226 152 2109 1697
Fragments arranged by size:
2109 1697 825 317 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3880 160
Size: 872 2848 1606
Fragments arranged by size:
2848 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5326
AvaI C'yCGr_G
Cuts at: 1870 1943 2133 2499 2651 1870
Size: 73 190 366 152 4545
Fragments arranged by size:
4545 366 190 152 73
BamHI G'GATC_C
Cuts at: 2604 2656 3023 2604
Size: 52 367 4907
Fragments arranged by size:
4907 367 52
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2369 2647 4102 618
Size: 325 217 627 582 278 1455 1842
Fragments arranged by size:
1842 1455 627 582 325 278 217
BanII G_rGCy'C
Cuts at: 1391 2134 2492 2645 1391
Size: 743 358 153 4072
Fragments arranged by size:
4072 743 358 153
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4355
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3517 3551 1903
Size: 34 1580 34 3678
Fragments arranged by size:
3678 1580 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3218 4428 3218
Size: 1210 4116
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5326
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3937 136
Size: 107 122 71 631 605 2265 1525
Fragments arranged by size:
2265 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5321 5321
Size: 5326
BlpI GC'TnA_GC
Cuts at: 2743 2743
Size: 5326
Bme1580I G_kGCm'C
Cuts at: 1790 2230 2492 3387 4543 1790
Size: 440 262 895 1156 2573
Fragments arranged by size:
2573 1156 895 440 262
BmgBI CAC'GTC
Cuts at: 1318 2518 1318
Size: 1200 4126
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3057 4015 442
Size: 416 53 83 399 24 1640 958 1753
Fragments arranged by size:
1753 1640 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5326
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2426 3986 1239
Size: 1187 1560 2579
Fragments arranged by size:
2579 1560 1187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3451 4316 4614 3451
Size: 865 298 4163
Fragments arranged by size:
4163 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3989 882
Size: 617 2490 2219
Fragments arranged by size:
2490 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2113 2455 3117 493
Size: 711 909 342 662 2702
Fragments arranged by size:
2702 909 711 662 342
BsaBI GATnn'nnATC
Cuts at: 5320 5320
Size: 5326
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3133 3515 4150 275
Size: 53 83 186 936 1600 382 635 1451
Fragments arranged by size:
1600 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2295 3759 4157 4431 4578 4989 2295
Size: 1464 398 274 147 411 2632
Fragments arranged by size:
2632 1464 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2372 2402 1178
Size: 30 165 30 969 30 4102
Fragments arranged by size:
4102 969 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 1186
Size: 916 4410
BseYI C'CCAG_C
Cuts at: 2256 4529 5025 2256
Size: 2273 496 2557
Fragments arranged by size:
2557 2273 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2011 1163
Size: 573 275 4478
Fragments arranged by size:
4478 573 275
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2480 2776 3537 4565 1540
Size: 275 186 479 296 761 1028 2301
Fragments arranged by size:
2301 1028 761 479 296 275 186
BsiHKAI G_wGCw'C
Cuts at: 3387 3472 4543 3387
Size: 85 1071 4170
Fragments arranged by size:
4170 1071 85
BsmI GAATG_Cn'
Cuts at: 2837 2930 2837
Size: 93 5233
BsmBI CGTCTCn'nnnn_
Cuts at: 2306 2306
Size: 5326
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2134 2230 2492 2645 3387 3472 4543
Size: 399 344 96 262 153 742 85 1071
Cuts at: 4543 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 344 262 153 96
85
BspEI T'CCGG_A
Cuts at: 2295 4989 2295
Size: 2694 2632
BspHI T'CATG_A
Cuts at: 2526 3215 4945 2526
Size: 689 1730 2907
Fragments arranged by size:
2907 1730 689
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2681 844
Size: 1837 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3213 1394
Size: 429 1390 3507
Fragments arranged by size:
3507 1390 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3821 3995 65
Size: 1183 2573 174 1396
Fragments arranged by size:
2573 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3970 4157 1700
Size: 110 2160 187 2869
Fragments arranged by size:
2869 2160 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5326
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5326
BssSI C'ACGA_G
Cuts at: 2351 3386 4398 2351
Size: 1035 1012 3279
Fragments arranged by size:
3279 1035 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2518 794
Size: 1724 3602
BstBI TT'CG_AA
Cuts at: 4130 4130
Size: 5326
BstEII G'GTnAC_C
Cuts at: 1184 2584 1184
Size: 1400 3926
BstYI r'GATC_y
Cuts at: 1972 2604 2656 3023 3408 3425 4877 4963 4975
Size: 632 52 367 385 17 1452 86 12
Cuts at: 4975 5321 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 632 385 367 346 86 52
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2230 2480 513
Size: 552 1165 250 3359
Fragments arranged by size:
3359 1165 552 250
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4587
Fragments arranged by size:
4587 683 56
BtsI GCAGTG_nn'
Cuts at: 2842 3636 3656 2842
Size: 794 20 4512
Fragments arranged by size:
4512 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5326
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5291
DraI TTT'AAA
Cuts at: 2630 2630
Size: 5326
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2593 4333 809
Size: 507 1277 1740 1802
Fragments arranged by size:
1802 1740 1277 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2477 2773 3662
Size: 54 1425 50 275 18 647 296 889
Cuts at: 3662 5291 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 647 296 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2477 2773 1537
Size: 275 665 296 4090
Fragments arranged by size:
4090 665 296 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3256 1353
Size: 1903 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2011 3913 4287 4433 387
Size: 1064 138 422 1902 374 146 1280
Fragments arranged by size:
1902 1280 1064 422 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2426 2543 3389 3986 4773 5041 1239
Size: 393 794 117 846 597 787 268 1524
Fragments arranged by size:
1524 846 794 787 597 393 268 117
EcoO109I rG'GnC_Cy
Cuts at: 2206 2488 2489 2595 2206
Size: 282 1 106 4937
Fragments arranged by size:
4937 282 106 1
EcoRI G'AATT_C
Cuts at: 2318 2635 3013 2318
Size: 317 378 4631
Fragments arranged by size:
4631 378 317
FspI TGC'GCA
Cuts at: 3832 3832
Size: 5326
HaeII r_GCGC'y
Cuts at: 1678 2018 2536 4473 1678
Size: 340 518 1937 2531
Fragments arranged by size:
2531 1937 518 340
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2116 2148 2584 2616 3967
Size: 32 135 32 544 32 436 32 1351
Cuts at: 3967 3999 4041 4073 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1351 544 436 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2670 2916 669
Size: 988 281 732 246 3079
Fragments arranged by size:
3079 988 732 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5326
HpaI GTT'AAC
Cuts at: 2916 2916
Size: 5326
KpnI G_GTAC'C
Cuts at: 2651 2651
Size: 5326
MfeI C'AATT_G
Cuts at: 2925 2925
Size: 5326
MluI A'CGCG_T
Cuts at: 5202 5202
Size: 5326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2582 4440 4624 1305
Size: 1277 1858 184 2007
Fragments arranged by size:
2007 1858 1277 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3504
Fragments arranged by size:
3504 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2111 2582 3284 3643 3802 518
Size: 648 945 471 702 359 159 2042
Fragments arranged by size:
2042 945 702 648 471 359 159
MspA1I CmG'CkG
Cuts at: 1606 2158 2232 2482 2742 3419 4567 4812 1606
Size: 552 74 250 260 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 552 260 250 245 74
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4774
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5326
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5326
NotI GC'GGCC_GC
Cuts at: 2477 2773 2477
Size: 296 5030
NspI r_CATG'y
Cuts at: 1132 1229 2684 4229 1132
Size: 97 1455 1545 2229
Fragments arranged by size:
2229 1545 1455 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5326
PciI A'CATG_T
Cuts at: 4225 4225
Size: 5326
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2209 1108
Size: 1101 4225
PmeI GTTT'AAAC
Cuts at: 2630 2630
Size: 5326
PmlI CAC'GTG
Cuts at: 2113 2113
Size: 5326
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2372 2404 3395 3427 4908 4940 2372
Size: 32 991 32 1481 32 2758
Fragments arranged by size:
2758 1481 991 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2206 2595 2206
Size: 389 4937
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5326
PsiI TTA'TAA
Cuts at: 2896 2896
Size: 5326
PspOMI G'GGCC_C
Cuts at: 2488 2488
Size: 5326
PstI C_TGCA'G
Cuts at: 830 2678 830
Size: 1848 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5326
PvuII CAG'CTG
Cuts at: 2158 2158
Size: 5326
SacII CC_GC'GG
Cuts at: 2233 2483 2233
Size: 250 5076
SalI G'TCGA_C
Cuts at: 1936 2668 1936
Size: 732 4594
SbfI CC_TGCA'GG
Cuts at: 2678 2678
Size: 5326
ScaI AGT'ACT
Cuts at: 2441 3574 5286 2441
Size: 1133 1712 2481
Fragments arranged by size:
2481 1712 1133
SfcI C'TryA_G
Cuts at: 826 1045 2176 2674 3809 4490 4681 826
Size: 219 1131 498 1135 681 191 1471
Fragments arranged by size:
1471 1135 1131 681 498 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5326
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5326
SmaI CCC'GGG
Cuts at: 1872 2501 2653 1872
Size: 629 152 4545
Fragments arranged by size:
4545 629 152
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3430 4331 4593 820
Size: 197 926 1487 901 262 1553
Fragments arranged by size:
1553 1487 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3117 493
Size: 2624 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5326
SphI G_CATG'C
Cuts at: 1132 2684 1132
Size: 1552 3774
SspI AAT'ATT
Cuts at: 5 52 3250 5
Size: 47 3198 2081
Fragments arranged by size:
3198 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5326
StyI C'CwwG_G
Cuts at: 513 1065 2541 2765 513
Size: 552 1476 224 3074
Fragments arranged by size:
3074 1476 552 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3353 3370 3523 3708 4153 325
Size: 1255 1773 17 153 185 445 1498
Fragments arranged by size:
1773 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2439 2574 3572
Size: 274 80 33 51 1443 462 135 998
Cuts at: 3572 5284 96
Size: 1712 138
Fragments arranged by size:
1712 1443 998 462 274 138 135 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5286 5318 5286
Size: 32 5294
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5326
XbaI T'CTAG_A
Cuts at: 2662 2662
Size: 5326
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5326
XmaI C'CCGG_G
Cuts at: 1870 2499 2651 1870
Size: 629 152 4545
Fragments arranged by size:
4545 629 152
XmnI GAAnn'nnTTC
Cuts at: 2567 3455 2567
Size: 888 4438
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3134 276
Size: 53 83 186 936 1600 2468
Fragments arranged by size:
2468 1600 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI