Restriction map for
pFN21AE0199
(Circular) (Six-base) MAPSORT of: pFN21AE0199.seq Check: 7980 from: 1 to: 8023
pFN21AE0199 8023 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2976 2976
Size: 8023
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5833 278
Size: 53 83 186 936 4297 2468
Fragments arranged by size:
4297 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4056 5366 1937
Size: 2119 1310 4594
Fragments arranged by size:
4594 2119 1310
Acc65I G'GTAC_C
Cuts at: 3581 5344 3581
Size: 1763 6260
AclI AA'CG_TT
Cuts at: 1563 6150 6523 1563
Size: 4587 373 3063
Fragments arranged by size:
4587 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2561 2978 3013 3450 3771 4230 4611 6086
Size: 929 417 35 437 321 459 381 1475
Cuts at: 6086 7470 7738 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1475 1384 929 459 437 417 381
321 268 35
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7826
AflIII A'CryG_T
Cuts at: 1203 2997 3148 6922 7899 1203
Size: 1794 151 3774 977 1327
Fragments arranged by size:
3774 1794 1327 977 151
AgeI A'CCGG_T
Cuts at: 3578 6854 3578
Size: 3276 4747
AhdI GACnn_n'nnGTC
Cuts at: 3549 6752 3549
Size: 3203 4820
AleI CACnn'nnGTG
Cuts at: 1166 2908 2982 4515 1166
Size: 1742 74 1533 4674
Fragments arranged by size:
4674 1742 1533 74
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8023
AlwNI CAG_nnn'CTG
Cuts at: 4223 5179 5766 7338 4223
Size: 956 587 1572 4908
Fragments arranged by size:
4908 1572 956 587
ApaI G_GGCC'C
Cuts at: 2086 2201 2439 3337 3608 4527 5244 2086
Size: 115 238 898 271 919 717 4865
Fragments arranged by size:
4865 919 898 717 271 238 115
ApaLI G'TGCA_C
Cuts at: 2762 2911 6080 7236 2762
Size: 149 3169 1156 3549
Fragments arranged by size:
3549 3169 1156 149
ApoI r'AATT_y
Cuts at: 1493 2738 4921 5303 5332 5558 5710 7819 1493
Size: 1245 2183 382 29 226 152 2109 1697
Fragments arranged by size:
2183 2109 1697 1245 382 226 152 29
AseI AT'TA_AT
Cuts at: 160 1032 6577 160
Size: 872 5545 1606
Fragments arranged by size:
5545 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8023
AvaI C'yCGr_G
Cuts at: 1870 1943 2484 2514 2666 3216 3546 5348 1870
Size: 73 541 30 152 550 330 1802 4545
Fragments arranged by size:
4545 1802 550 541 330 152 73 30
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3565 3573 3598 3606 3565
Size: 8 25 8 7982
Fragments arranged by size:
7982 25 8 8
BamHI G'GATC_C
Cuts at: 2629 5353 5720 2629
Size: 2724 367 4932
Fragments arranged by size:
4932 2724 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2384 2925 3192 3220 3305
Size: 325 217 627 597 541 267 28 85
Cuts at: 3305 3581 4461 4492 5344 6799 618
Size: 276 880 31 852 1455 1842
Fragments arranged by size:
1842 1455 880 852 627 597 541 325
276 267 217 85 31 28
BanII G_rGCy'C
Cuts at: 1391 2052 2086 2201 2396 2439 3246 3337 3608
Size: 661 34 115 195 43 807 91 271
Cuts at: 3608 4527 5172 5244 5342 1391
Size: 919 645 72 98 4072
Fragments arranged by size:
4072 919 807 661 645 271 195 115
98 91 72 43 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2966 3438 3839 928
Size: 971 1067 472 401 5112
Fragments arranged by size:
5112 1067 971 472 401
BbvCI CC'TCA_GC
Cuts at: 2840 2840
Size: 8023
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3044 3078 6214 6248 1903
Size: 34 1107 34 3136 34 3678
Fragments arranged by size:
3678 3136 1107 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3881 3891 5915 7125 3881
Size: 10 2024 1210 4779
Fragments arranged by size:
4779 2024 1210 10
BclI T'GATC_A
Cuts at: 1548 5200 1548
Size: 3652 4371
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3603 4264 5176
Size: 107 122 71 631 605 1931 661 912
Cuts at: 5176 6634 136
Size: 1458 1525
Fragments arranged by size:
1931 1525 1458 912 661 631 605 122
107 71
BglII A'GATC_T
Cuts at: 8018 8018
Size: 8023
BlpI GC'TnA_GC
Cuts at: 4224 4689 5440 4224
Size: 465 751 6807
Fragments arranged by size:
6807 751 465
Bme1580I G_kGCm'C
Cuts at: 1790 2086 2201 2251 2439 2766 2915 3223 3337
Size: 296 115 50 188 327 149 308 114
Cuts at: 3337 3608 4466 4527 5244 6084 7240 1790
Size: 271 858 61 717 840 1156 2573
Fragments arranged by size:
2573 1156 858 840 717 327 308 296
271 188 149 115 114 61 50
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8023
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2067 2082 2827
Size: 416 53 83 399 24 650 15 745
Cuts at: 2827 2851 3331 3605 4460 4509 5754 6712 442
Size: 24 480 274 855 49 1245 958 1753
Fragments arranged by size:
1753 1245 958 855 745 650 480 416
399 274 83 53 49 24 24 15
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8023
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2415 5205 2415
Size: 2790 5233
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2337 2815 3424 3625 3939 4568 4818 6683
Size: 1098 478 609 201 314 629 250 1865
Cuts at: 6683 1239
Size: 2579
Fragments arranged by size:
2579 1865 1098 629 609 478 314 250
201
Bpu10I CC'TnA_GC
Cuts at: 2840 2840
Size: 8023
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3518 4667 6148 7013 7311 3518
Size: 1149 1481 865 298 4230
Fragments arranged by size:
4230 1481 1149 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2171 3557 6686 882
Size: 617 672 1386 3129 2219
Fragments arranged by size:
3129 2219 1386 672 617
BsaAI yAC'GTr
Cuts at: 493 1204 5814 493
Size: 711 4610 2702
Fragments arranged by size:
4610 2702 711
BsaBI GATnn'nnATC
Cuts at: 2347 8017 2347
Size: 5670 2353
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3174 5830 6212 6847
Size: 53 83 186 936 1641 2656 382 635
Cuts at: 6847 275
Size: 1451
Fragments arranged by size:
2656 1641 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3578 6456 6854 7128 7275 7686 3578
Size: 2878 398 274 147 411 3915
Fragments arranged by size:
3915 2878 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2795 2825 3513 3543 3907
Size: 30 165 30 1392 30 688 30 364
Cuts at: 3907 3937 4715 4745 5215 5245 1178
Size: 30 778 30 470 30 3956
Fragments arranged by size:
3956 1392 778 688 470 364 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2690 3670 4560 5076 5178 1186
Size: 1504 980 890 516 102 4031
Fragments arranged by size:
4031 1504 980 890 516 102
BseYI C'CCAG_C
Cuts at: 2312 2410 3563 3756 3928 4073 4305 4560 4602
Size: 98 1153 193 172 145 232 255 42
Cuts at: 4602 5030 7226 7722 2312
Size: 428 2196 496 2613
Fragments arranged by size:
2613 2196 1153 496 428 255 232 193
172 145 98 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2173 2244 3094 3396 1163
Size: 573 437 71 850 302 5790
Fragments arranged by size:
5790 850 573 437 302 71
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5473 6234 7262 1540
Size: 275 186 3472 761 1028 2301
Fragments arranged by size:
3472 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2766 2784 2915 3009 6084 6169 7240 2766
Size: 18 131 94 3075 85 1071 3549
Fragments arranged by size:
3549 3075 1071 131 94 85 18
BsmI GAATG_Cn'
Cuts at: 4093 5534 5627 4093
Size: 1441 93 6489
Fragments arranged by size:
6489 1441 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2086 2201 2251 2396 2439 2766
Size: 399 262 34 115 50 145 43 327
Cuts at: 2766 2784 2915 3009 3223 3246 3337 3608 4466
Size: 18 131 94 214 23 91 271 858
Cuts at: 4466 4527 5172 5244 5342 6084 6169 7240 1391
Size: 61 645 72 98 742 85 1071 2174
Fragments arranged by size:
2174 1071 858 742 645 399 327 271
262 214 145 131 115 98 94 91
85 72 61 50 43 34 23 18
BspEI T'CCGG_A
Cuts at: 7686 7686
Size: 8023
BspHI T'CATG_A
Cuts at: 5912 7642 5912
Size: 1730 6293
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2295 2976 3774 5378 844
Size: 1451 681 798 1604 3489
Fragments arranged by size:
3489 1604 1451 798 681
BsrBI CCG'CTC
Cuts at: 1394 1823 2797 3256 3678 5910 1394
Size: 429 974 459 422 2232 3507
Fragments arranged by size:
3507 2232 974 459 429 422
BsrDI GCAATG_nn'
Cuts at: 65 1248 3040 3418 3462 4985 6518 6692 65
Size: 1183 1792 378 44 1523 1533 174 1396
Fragments arranged by size:
1792 1533 1523 1396 1183 378 174 44
BsrFI r'CCGG_y
Cuts at: 1700 1810 3578 6667 6854 1700
Size: 110 1768 3089 187 2869
Fragments arranged by size:
3089 2869 1768 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8023
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8023
BssSI C'ACGA_G
Cuts at: 3008 6083 7095 3008
Size: 3075 1012 3936
Fragments arranged by size:
3936 3075 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2761 3374 3766 3782 794
Size: 1967 613 392 16 5035
Fragments arranged by size:
5035 1967 613 392 16
BstBI TT'CG_AA
Cuts at: 6827 6827
Size: 8023
BstEII G'GTnAC_C
Cuts at: 1184 3907 1184
Size: 2723 5300
BstXI CCAn_nnnn'nTGG
Cuts at: 2162 2465 4489 4795 2162
Size: 303 2024 306 5390
Fragments arranged by size:
5390 2024 306 303
BstYI r'GATC_y
Cuts at: 1972 2629 3127 5282 5353 5720 6105 6122 7574
Size: 657 498 2155 71 367 385 17 1452
Cuts at: 7574 7660 7672 8018 1972
Size: 86 12 346 1977
Fragments arranged by size:
2155 1977 1452 657 498 385 367 346
86 71 17 12
Bsu36I CC'TnA_GG
Cuts at: 3506 5046 3506
Size: 1540 6483
BtgI C'CryG_G
Cuts at: 513 1065 2788 3196 4095 4140 513
Size: 552 1723 408 899 45 4396
Fragments arranged by size:
4396 1723 899 552 408 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2356 487
Size: 683 56 1130 6154
Fragments arranged by size:
6154 1130 683 56
BtsI GCAGTG_nn'
Cuts at: 3358 3813 4156 5539 6333 6353 3358
Size: 455 343 1383 794 20 5028
Fragments arranged by size:
5028 1383 794 455 343 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8023
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7988
DraI TTT'AAA
Cuts at: 5327 5327
Size: 8023
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7030 809
Size: 507 5714 1802
Fragments arranged by size:
5714 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3533 5470 6359
Size: 54 1425 50 275 18 1703 1937 889
Cuts at: 6359 7988 8
Size: 1629 43
Fragments arranged by size:
1937 1703 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5470 1537
Size: 275 3658 4090
Fragments arranged by size:
4090 3658 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3492 4113 5953 1353
Size: 2139 621 1840 3423
Fragments arranged by size:
3423 2139 1840 621
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6610 6984 7130 387
Size: 1064 138 5021 374 146 1280
Fragments arranged by size:
5021 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2337 2561 2815 2978 3013 3424 3450
Size: 393 705 224 254 163 35 411 26
Cuts at: 3450 3625 3771 3939 4230 4568 4611 4818 6086
Size: 175 146 168 291 338 43 207 1268
Cuts at: 6086 6683 7470 7738 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1268 787 705 597 411 393 338
291 268 254 224 207 175 168 163
146 43 35 26
EcoNI CCTnn'n_nnAGG
Cuts at: 5091 5091
Size: 8023
EcoO109I rG'GnC_Cy
Cuts at: 2082 2083 2197 2435 2436 2519 2662 2844 3266
Size: 1 114 238 1 83 143 182 422
Cuts at: 3266 3333 3455 3543 3605 4134 4205 4379 4523
Size: 67 122 88 62 529 71 174 144
Cuts at: 4523 4524 4592 5069 5241 2082
Size: 1 68 477 172 4864
Fragments arranged by size:
4864 529 477 422 238 182 174 172
144 143 122 114 88 83 71 68
67 62 1 1 1
EcoRI G'AATT_C
Cuts at: 5303 5332 5710 5303
Size: 29 378 7616
Fragments arranged by size:
7616 378 29
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3801 3801
Size: 8023
FspI TGC'GCA
Cuts at: 2882 6529 2882
Size: 3647 4376
HaeII r_GCGC'y
Cuts at: 1678 7170 1678
Size: 5492 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2488 2520 2678 2710 3119
Size: 32 135 32 916 32 158 32 409
Cuts at: 3119 3151 3255 3287 3535 3567 3829 3861 6664
Size: 32 104 32 248 32 262 32 2803
Cuts at: 6664 6696 6738 6770 1373
Size: 32 42 32 2626
Fragments arranged by size:
2803 2626 916 409 262 248 158 135
104 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2235 5367 5613 669
Size: 988 281 297 3132 246 3079
Fragments arranged by size:
3132 3079 988 297 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8023
HpaI GTT'AAC
Cuts at: 5613 5613
Size: 8023
KpnI G_GTAC'C
Cuts at: 3585 5348 3585
Size: 1763 6260
MfeI C'AATT_G
Cuts at: 5622 5622
Size: 8023
MluI A'CGCG_T
Cuts at: 7899 7899
Size: 8023
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2250 7137 7321 1305
Size: 945 4887 184 2007
Fragments arranged by size:
4887 2007 945 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6201
Fragments arranged by size:
6201 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2160 2867 2908 2982 2998 3075 4515
Size: 648 994 707 41 74 16 77 1440
Cuts at: 4515 5255 5981 6340 6499 518
Size: 740 726 359 159 2042
Fragments arranged by size:
2042 1440 994 740 726 707 648 359
159 77 74 41 16
MspA1I CmG'CkG
Cuts at: 1606 2187 2539 2758 4223 5034 5143 5206 5439
Size: 581 352 219 1465 811 109 63 233
Cuts at: 5439 6116 7264 7509 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1465 1148 811 677 581 352 245
233 219 109 63
NcoI C'CATG_G
Cuts at: 513 1065 4095 4140 513
Size: 552 3030 45 4396
Fragments arranged by size:
4396 3030 552 45
NdeI CA'TA_TG
Cuts at: 387 4846 387
Size: 4459 3564
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8023
NotI GC'GGCC_GC
Cuts at: 5470 5470
Size: 8023
NruI TCG'CGA
Cuts at: 2341 2341
Size: 8023
NspI r_CATG'y
Cuts at: 1132 1229 2560 2866 2881 3001 3152 5381 6926
Size: 97 1331 306 15 120 151 2229 1545
Cuts at: 6926 1132
Size: 2229
Fragments arranged by size:
2229 2229 1545 1331 306 151 120 97
15
PasI CC'CwG_GG
Cuts at: 1348 3329 5173 1348
Size: 1981 1844 4198
Fragments arranged by size:
4198 1981 1844
PciI A'CATG_T
Cuts at: 2997 3148 6922 2997
Size: 151 3774 4098
Fragments arranged by size:
4098 3774 151
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3329 3722 4130 1108
Size: 2221 393 408 5001
Fragments arranged by size:
5001 2221 408 393
PfoI T'CCnGG_A
Cuts at: 2353 3883 2353
Size: 1530 6493
PmeI GTTT'AAAC
Cuts at: 5327 5327
Size: 8023
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6092 6124 7605 7637 6092
Size: 32 1481 32 6478
Fragments arranged by size:
6478 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2519 2662 3266 3455 3543 4134 4205 5069 2519
Size: 143 604 189 88 591 71 864 5473
Fragments arranged by size:
5473 864 604 591 189 143 88 71
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8023
PsiI TTA'TAA
Cuts at: 5593 5593
Size: 8023
PspOMI G'GGCC_C
Cuts at: 2082 2197 2435 3333 3604 4523 5240 2082
Size: 115 238 898 271 919 717 4865
Fragments arranged by size:
4865 919 898 717 271 238 115
PstI C_TGCA'G
Cuts at: 830 2292 2732 3020 3350 3820 5375 830
Size: 1462 440 288 330 470 1555 3478
Fragments arranged by size:
3478 1555 1462 470 440 330 288
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8023
PvuII CAG'CTG
Cuts at: 2187 2539 2758 4223 5034 5143 2187
Size: 352 219 1465 811 109 5067
Fragments arranged by size:
5067 1465 811 352 219 109
SalI G'TCGA_C
Cuts at: 1936 5365 1936
Size: 3429 4594
SanDI GG'GwC_CC
Cuts at: 2519 3543 2519
Size: 1024 6999
SapI GCTCTTCn'nnn_
Cuts at: 4113 4113
Size: 8023
SbfI CC_TGCA'GG
Cuts at: 5375 5375
Size: 8023
ScaI AGT'ACT
Cuts at: 6271 7983 6271
Size: 1712 6311
SfcI C'TryA_G
Cuts at: 826 1045 2288 2728 3016 3234 3346 3816 4112
Size: 219 1243 440 288 218 112 470 296
Cuts at: 4112 4402 5371 6506 7187 7378 826
Size: 290 969 1135 681 191 1471
Fragments arranged by size:
1471 1243 1135 969 681 470 440 296
290 288 219 218 191 112
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8023
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8023
SmaI CCC'GGG
Cuts at: 1872 2486 2516 3548 5350 1872
Size: 614 30 1032 1802 4545
Fragments arranged by size:
4545 1802 1032 614 30
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2666 3497 4682 6127 7028 7290
Size: 197 926 723 831 1185 1445 901 262
Cuts at: 7290 820
Size: 1553
Fragments arranged by size:
1553 1445 1185 926 901 831 723 262
197
SnaBI TAC'GTA
Cuts at: 493 5814 493
Size: 5321 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8023
SphI G_CATG'C
Cuts at: 1132 5381 1132
Size: 4249 3774
SspI AAT'ATT
Cuts at: 5 52 5947 5
Size: 47 5895 2081
Fragments arranged by size:
5895 2081 47
StuI AGG'CCT
Cuts at: 1517 4313 4639 1517
Size: 2796 326 4901
Fragments arranged by size:
4901 2796 326
StyI C'CwwG_G
Cuts at: 513 1065 2586 3860 4095 4140 4383 5462 513
Size: 552 1521 1274 235 45 243 1079 3074
Fragments arranged by size:
3074 1521 1274 1079 552 243 235 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2326 3060 4018 4291 4346 4679 6050
Size: 1255 746 734 958 273 55 333 1371
Cuts at: 6050 6067 6220 6405 6850 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1371 1255 958 746 734 445 333
273 185 153 55 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2859 6269 7981
Size: 274 80 33 51 1443 882 3410 1712
Cuts at: 7981 96
Size: 138
Fragments arranged by size:
3410 1712 1443 882 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2795 2827 3907 3939 4715 4747 7983 8015 2795
Size: 32 1080 32 776 32 3236 32 2803
Fragments arranged by size:
3236 2803 1080 776 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8023
XbaI T'CTAG_A
Cuts at: 5359 5359
Size: 8023
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3235 4642 5254 3235
Size: 1407 612 6004
Fragments arranged by size:
6004 1407 612
XhoI C'TCGA_G
Cuts at: 1943 2666 1943
Size: 723 7300
XmaI C'CCGG_G
Cuts at: 1870 2484 2514 3546 5348 1870
Size: 614 30 1032 1802 4545
Fragments arranged by size:
4545 1802 1032 614 30
XmnI GAAnn'nnTTC
Cuts at: 2804 6152 2804
Size: 3348 4675
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5831 276
Size: 53 83 186 936 4297 2468
Fragments arranged by size:
4297 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SexAI SfiI SfoI SrfI SwaI