Restriction map for
pFN21AE0197
(Circular) (Six-base) MAPSORT of: pFN21AE0197.seq Check: 2304 from: 1 to: 5062
pFN21AE0197 5062 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2872 278
Size: 53 83 186 936 1336 2468
Fragments arranged by size:
2468 1336 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2405 1937
Size: 468 4594
Acc65I G'GTAC_C
Cuts at: 2383 2383
Size: 5062
AclI AA'CG_TT
Cuts at: 1563 2361 3189 3562 1563
Size: 798 828 373 3063
Fragments arranged by size:
3063 828 798 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2159 2213 3125 4509 4777 1632
Size: 527 54 912 1384 268 1917
Fragments arranged by size:
1917 1384 912 527 268 54
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4865
AflIII A'CryG_T
Cuts at: 1203 3961 4938 1203
Size: 2758 977 1327
Fragments arranged by size:
2758 1327 977
AgeI A'CCGG_T
Cuts at: 3893 3893
Size: 5062
AhdI GACnn_n'nnGTC
Cuts at: 3791 3791
Size: 5062
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5062
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5062
AlwNI CAG_nnn'CTG
Cuts at: 2805 4377 2805
Size: 1572 3490
ApaLI G'TGCA_C
Cuts at: 3119 4275 3119
Size: 1156 3906
ApoI r'AATT_y
Cuts at: 1493 2148 2371 2597 2749 4858 1493
Size: 655 223 226 152 2109 1697
Fragments arranged by size:
2109 1697 655 226 223 152
AseI AT'TA_AT
Cuts at: 160 1032 3616 160
Size: 872 2584 1606
Fragments arranged by size:
2584 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5062
AvaI C'yCGr_G
Cuts at: 1870 1943 2387 1870
Size: 73 444 4545
Fragments arranged by size:
4545 444 73
BamHI G'GATC_C
Cuts at: 2392 2759 2392
Size: 367 4695
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2383 3838 618
Size: 325 217 627 596 1455 1842
Fragments arranged by size:
1842 1455 627 596 325 217
BanII G_rGCy'C
Cuts at: 1391 2381 1391
Size: 990 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4091
BbvCI CC'TCA_GC
Cuts at: 2057 2057
Size: 5062
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3253 3287 1903
Size: 34 1316 34 3678
Fragments arranged by size:
3678 1316 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2954 4164 2954
Size: 1210 3852
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5062
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3673 136
Size: 107 122 71 631 605 2001 1525
Fragments arranged by size:
2001 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5057 5057
Size: 5062
BlpI GC'TnA_GC
Cuts at: 2479 2479
Size: 5062
Bme1580I G_kGCm'C
Cuts at: 1790 2173 3123 4279 1790
Size: 383 950 1156 2573
Fragments arranged by size:
2573 1156 950 383
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5062
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2793 3751 442
Size: 416 53 83 399 24 1376 958 1753
Fragments arranged by size:
1753 1376 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5062
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2273 3722 1239
Size: 1034 1449 2579
Fragments arranged by size:
2579 1449 1034
Bpu10I CC'TnA_GC
Cuts at: 2057 2081 2057
Size: 24 5038
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3187 4052 4350 3187
Size: 865 298 3899
Fragments arranged by size:
3899 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3725 882
Size: 617 2226 2219
Fragments arranged by size:
2226 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2853 493
Size: 711 1649 2702
Fragments arranged by size:
2702 1649 711
BsaBI GATnn'nnATC
Cuts at: 5056 5056
Size: 5062
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2869 3251 3886 275
Size: 53 83 186 936 1336 382 635 1451
Fragments arranged by size:
1451 1336 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3495 3893 4167 4314 4725 3495
Size: 398 274 147 411 3832
Fragments arranged by size:
3832 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4837
Fragments arranged by size:
4837 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5062
BseYI C'CCAG_C
Cuts at: 4265 4761 4265
Size: 496 4566
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4489
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2512 3273 4301 1540
Size: 275 186 511 761 1028 2301
Fragments arranged by size:
2301 1028 761 511 275 186
BsiHKAI G_wGCw'C
Cuts at: 3123 3208 4279 3123
Size: 85 1071 3906
Fragments arranged by size:
3906 1071 85
BsmI GAATG_Cn'
Cuts at: 2573 2666 2573
Size: 93 4969
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2173 2381 3123 3208 4279 1391
Size: 399 383 208 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 383 208 85
BspEI T'CCGG_A
Cuts at: 4725 4725
Size: 5062
BspHI T'CATG_A
Cuts at: 2951 4681 2951
Size: 1730 3332
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2417 844
Size: 1573 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2949 1394
Size: 429 1126 3507
Fragments arranged by size:
3507 1126 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3557 3731 65
Size: 1183 2309 174 1396
Fragments arranged by size:
2309 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3706 3893 1700
Size: 110 1896 187 2869
Fragments arranged by size:
2869 1896 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5062
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5062
BssSI C'ACGA_G
Cuts at: 2036 3122 4134 2036
Size: 1086 1012 2964
Fragments arranged by size:
2964 1086 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5062
BstBI TT'CG_AA
Cuts at: 2109 3866 2109
Size: 1757 3305
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5062
BstXI CCAn_nnnn'nTGG
Cuts at: 2020 2020
Size: 5062
BstYI r'GATC_y
Cuts at: 1972 2392 2759 3144 3161 4613 4699 4711 5057
Size: 420 367 385 17 1452 86 12 346
Cuts at: 5057 1972
Size: 1977
Fragments arranged by size:
1977 1452 420 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4510
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4323
Fragments arranged by size:
4323 683 56
BtsI GCAGTG_nn'
Cuts at: 2578 3372 3392 2578
Size: 794 20 4248
Fragments arranged by size:
4248 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5062
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5027
DraI TTT'AAA
Cuts at: 2265 2366 2265
Size: 101 4961
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4069 809
Size: 507 2753 1802
Fragments arranged by size:
2753 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2509 3398 5027
Size: 54 1425 50 275 18 679 889 1629
Cuts at: 5027 8
Size: 43
Fragments arranged by size:
1629 1425 889 679 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2509 1537
Size: 275 697 4090
Fragments arranged by size:
4090 697 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2327 2992 1353
Size: 974 665 3423
Fragments arranged by size:
3423 974 665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3649 4023 4169 387
Size: 1064 138 2060 374 146 1280
Fragments arranged by size:
2060 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2213 2273 3125 3722 4509 4777
Size: 393 527 54 60 852 597 787 268
Cuts at: 4777 1239
Size: 1524
Fragments arranged by size:
1524 852 787 597 527 393 268 60
54
EcoRI G'AATT_C
Cuts at: 2371 2749 2371
Size: 378 4684
FspI TGC'GCA
Cuts at: 3568 3568
Size: 5062
HaeII r_GCGC'y
Cuts at: 1678 4209 1678
Size: 2531 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2118 2150 3703 3735 3777
Size: 32 135 32 546 32 1553 32 42
Cuts at: 3777 3809 1373
Size: 32 2626
Fragments arranged by size:
2626 1553 546 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2406 2652 669
Size: 988 281 468 246 3079
Fragments arranged by size:
3079 988 468 281 246
HindIII A'AGCT_T
Cuts at: 748 2259 748
Size: 1511 3551
HpaI GTT'AAC
Cuts at: 2652 2652
Size: 5062
KpnI G_GTAC'C
Cuts at: 2387 2387
Size: 5062
MfeI C'AATT_G
Cuts at: 2661 2661
Size: 5062
MluI A'CGCG_T
Cuts at: 4938 4938
Size: 5062
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4176 4360 1305
Size: 2871 184 2007
Fragments arranged by size:
2871 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3240
Fragments arranged by size:
3240 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3020 3379 3538 518
Size: 648 1854 359 159 2042
Fragments arranged by size:
2042 1854 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2478 3155 4303 4548 1606
Size: 872 677 1148 245 2120
Fragments arranged by size:
2120 1148 872 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4510
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5062
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5062
NotI GC'GGCC_GC
Cuts at: 2509 2509
Size: 5062
NspI r_CATG'y
Cuts at: 1132 1229 2420 3965 1132
Size: 97 1191 1545 2229
Fragments arranged by size:
2229 1545 1191 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5062
PciI A'CATG_T
Cuts at: 3961 3961
Size: 5062
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5062
PmeI GTTT'AAAC
Cuts at: 2366 2366
Size: 5062
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3131 3163 4644 4676 3131
Size: 32 1481 32 3517
Fragments arranged by size:
3517 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5062
PsiI TTA'TAA
Cuts at: 2632 2632
Size: 5062
PstI C_TGCA'G
Cuts at: 830 2414 830
Size: 1584 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5062
SalI G'TCGA_C
Cuts at: 1936 2404 1936
Size: 468 4594
SbfI CC_TGCA'GG
Cuts at: 2414 2414
Size: 5062
ScaI AGT'ACT
Cuts at: 3310 5022 3310
Size: 1712 3350
SfcI C'TryA_G
Cuts at: 826 1045 2086 2410 3545 4226 4417 826
Size: 219 1041 324 1135 681 191 1471
Fragments arranged by size:
1471 1135 1041 681 324 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5062
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5062
SmaI CCC'GGG
Cuts at: 1872 2389 1872
Size: 517 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3166 4067 4329 820
Size: 197 926 1223 901 262 1553
Fragments arranged by size:
1553 1223 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2853 493
Size: 2360 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5062
SphI G_CATG'C
Cuts at: 1132 2420 1132
Size: 1288 3774
SspI AAT'ATT
Cuts at: 5 52 2986 5
Size: 47 2934 2081
Fragments arranged by size:
2934 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5062
StyI C'CwwG_G
Cuts at: 513 1065 2013 2501 513
Size: 552 948 488 3074
Fragments arranged by size:
3074 948 552 488
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3089 3106 3259 3444 3889 325
Size: 1255 1509 17 153 185 445 1498
Fragments arranged by size:
1509 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3308 5020 96
Size: 274 80 33 51 1443 1331 1712 138
Fragments arranged by size:
1712 1443 1331 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5022 5054 5022
Size: 32 5030
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5062
XbaI T'CTAG_A
Cuts at: 2398 2398
Size: 5062
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5062
XmaI C'CCGG_G
Cuts at: 1870 2387 1870
Size: 517 4545
XmnI GAAnn'nnTTC
Cuts at: 3191 3191
Size: 5062
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2870 276
Size: 53 83 186 936 1336 2468
Fragments arranged by size:
2468 1336 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI