Restriction map for
pFN21AE0186
(Circular) (Six-base) MAPSORT of: pFN21AE0186.seq Check: 4742 from: 1 to: 5365
pFN21AE0186 5365 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3175 278
Size: 53 83 186 936 1639 2468
Fragments arranged by size:
2468 1639 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2708 1937
Size: 771 4594
Acc65I G'GTAC_C
Cuts at: 2686 2686
Size: 5365
AclI AA'CG_TT
Cuts at: 1563 2044 3492 3865 1563
Size: 481 1448 373 3063
Fragments arranged by size:
3063 1448 481 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2399 3428 4812 5080 1632
Size: 767 1029 1384 268 1917
Fragments arranged by size:
1917 1384 1029 767 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5168
AflIII A'CryG_T
Cuts at: 1203 2130 2204 4264 5241 1203
Size: 927 74 2060 977 1327
Fragments arranged by size:
2060 1327 977 927 74
AgeI A'CCGG_T
Cuts at: 4196 4196
Size: 5365
AhdI GACnn_n'nnGTC
Cuts at: 4094 4094
Size: 5365
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5365
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5365
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2643 2675 2643
Size: 32 5333
AlwNI CAG_nnn'CTG
Cuts at: 3108 4680 3108
Size: 1572 3793
ApaLI G'TGCA_C
Cuts at: 3422 4578 3422
Size: 1156 4209
ApoI r'AATT_y
Cuts at: 1493 2057 2513 2536 2674 2900 3052 5161 1493
Size: 564 456 23 138 226 152 2109 1697
Fragments arranged by size:
2109 1697 564 456 226 152 138 23
AseI AT'TA_AT
Cuts at: 160 1032 3919 160
Size: 872 2887 1606
Fragments arranged by size:
2887 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5365
AvaI C'yCGr_G
Cuts at: 1870 1943 2690 1870
Size: 73 747 4545
Fragments arranged by size:
4545 747 73
BamHI G'GATC_C
Cuts at: 2695 3062 2695
Size: 367 4998
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2686 4141 618
Size: 325 217 627 899 1455 1842
Fragments arranged by size:
1842 1455 899 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2684 1391
Size: 1293 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2201 928
Size: 971 302 4092
Fragments arranged by size:
4092 971 302
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3556 3590 1903
Size: 34 1619 34 3678
Fragments arranged by size:
3678 1619 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3257 4467 3257
Size: 1210 4155
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5365
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3976 136
Size: 107 122 71 631 605 2304 1525
Fragments arranged by size:
2304 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5360 5360
Size: 5365
BlpI GC'TnA_GC
Cuts at: 2582 2782 2582
Size: 200 5165
Bme1580I G_kGCm'C
Cuts at: 1790 3426 4582 1790
Size: 1636 1156 2573
Fragments arranged by size:
2573 1636 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5365
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3096 4054 442
Size: 416 53 83 399 24 1679 958 1753
Fragments arranged by size:
1753 1679 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5365
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4025 1239
Size: 2786 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3490 4355 4653 3490
Size: 865 298 4202
Fragments arranged by size:
4202 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2095 2359 4028 882
Size: 617 596 264 1669 2219
Fragments arranged by size:
2219 1669 617 596 264
BsaAI yAC'GTr
Cuts at: 493 1204 3156 493
Size: 711 1952 2702
Fragments arranged by size:
2702 1952 711
BsaBI GATnn'nnATC
Cuts at: 5359 5359
Size: 5365
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3172 3554 4189 275
Size: 53 83 186 936 1639 382 635 1451
Fragments arranged by size:
1639 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3798 4196 4470 4617 5028 3798
Size: 398 274 147 411 4135
Fragments arranged by size:
4135 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5140
Fragments arranged by size:
5140 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2234 1186
Size: 1048 4317
BseYI C'CCAG_C
Cuts at: 4568 5064 4568
Size: 496 4869
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2073 1163
Size: 573 337 4455
Fragments arranged by size:
4455 573 337
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2815 3576 4604 1540
Size: 275 186 814 761 1028 2301
Fragments arranged by size:
2301 1028 814 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3426 3511 4582 3426
Size: 85 1071 4209
Fragments arranged by size:
4209 1071 85
BsmI GAATG_Cn'
Cuts at: 2876 2969 2876
Size: 93 5272
BsmBI CGTCTCn'nnnn_
Cuts at: 2646 2646
Size: 5365
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2684 3426 3511 4582 1391
Size: 399 894 742 85 1071 2174
Fragments arranged by size:
2174 1071 894 742 399 85
BspEI T'CCGG_A
Cuts at: 5028 5028
Size: 5365
BspHI T'CATG_A
Cuts at: 2401 2517 3254 4984 2401
Size: 116 737 1730 2782
Fragments arranged by size:
2782 1730 737 116
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2720 844
Size: 1876 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3252 1394
Size: 429 1429 3507
Fragments arranged by size:
3507 1429 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3860 4034 65
Size: 1183 2612 174 1396
Fragments arranged by size:
2612 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4009 4196 1700
Size: 110 2199 187 2869
Fragments arranged by size:
2869 2199 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5365
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5365
BssSI C'ACGA_G
Cuts at: 3425 4437 3425
Size: 1012 4353
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5365
BstBI TT'CG_AA
Cuts at: 4169 4169
Size: 5365
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5365
BstYI r'GATC_y
Cuts at: 1972 2695 3062 3447 3464 4916 5002 5014 5360
Size: 723 367 385 17 1452 86 12 346
Cuts at: 5360 1972
Size: 1977
Fragments arranged by size:
1977 1452 723 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2194 2214 2435 513
Size: 552 939 190 20 221 3443
Fragments arranged by size:
3443 939 552 221 190 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4626
Fragments arranged by size:
4626 683 56
BtsI GCAGTG_nn'
Cuts at: 2881 3675 3695 2881
Size: 794 20 4551
Fragments arranged by size:
4551 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5365
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5330
DraI TTT'AAA
Cuts at: 2669 2669
Size: 5365
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2205 4372 809
Size: 507 889 2167 1802
Fragments arranged by size:
2167 1802 889 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2812 3701 5330
Size: 54 1425 50 275 18 982 889 1629
Cuts at: 5330 8
Size: 43
Fragments arranged by size:
1629 1425 982 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2812 1537
Size: 275 1000 4090
Fragments arranged by size:
4090 1000 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3295 1353
Size: 1942 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3952 4326 4472 387
Size: 1064 138 2363 374 146 1280
Fragments arranged by size:
2363 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2399 3428 4025 4812 5080 1239
Size: 393 767 1029 597 787 268 1524
Fragments arranged by size:
1524 1029 787 767 597 393 268
EcoRI G'AATT_C
Cuts at: 2057 2513 2536 2674 3052 2057
Size: 456 23 138 378 4370
Fragments arranged by size:
4370 456 378 138 23
FspI TGC'GCA
Cuts at: 3871 3871
Size: 5365
HaeII r_GCGC'y
Cuts at: 1678 4512 1678
Size: 2834 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2161 2193 4006 4038 4080
Size: 32 135 32 589 32 1813 32 42
Cuts at: 4080 4112 1373
Size: 32 2626
Fragments arranged by size:
2626 1813 589 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2563 2709 2955 669
Size: 988 281 625 146 246 3079
Fragments arranged by size:
3079 988 625 281 246 146
HindIII A'AGCT_T
Cuts at: 748 2350 748
Size: 1602 3763
HpaI GTT'AAC
Cuts at: 2955 2955
Size: 5365
KpnI G_GTAC'C
Cuts at: 2690 2690
Size: 5365
MfeI C'AATT_G
Cuts at: 2964 2964
Size: 5365
MluI A'CGCG_T
Cuts at: 5241 5241
Size: 5365
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2065 4479 4663 1305
Size: 760 2414 184 2007
Fragments arranged by size:
2414 2007 760 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3543
Fragments arranged by size:
3543 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3323 3682 3841 518
Size: 648 2157 359 159 2042
Fragments arranged by size:
2157 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2781 3458 4606 4851 1606
Size: 1175 677 1148 245 2120
Fragments arranged by size:
2120 1175 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2194 2214 2435 513
Size: 552 939 190 20 221 3443
Fragments arranged by size:
3443 939 552 221 190 20
NdeI CA'TA_TG
Cuts at: 387 2387 387
Size: 2000 3365
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5365
NotI GC'GGCC_GC
Cuts at: 2812 2812
Size: 5365
NspI r_CATG'y
Cuts at: 1132 1229 2134 2208 2723 4268 1132
Size: 97 905 74 515 1545 2229
Fragments arranged by size:
2229 1545 905 515 97 74
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5365
PciI A'CATG_T
Cuts at: 2130 2204 4264 2130
Size: 74 2060 3231
Fragments arranged by size:
3231 2060 74
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2471 2603 1108
Size: 1363 132 3870
Fragments arranged by size:
3870 1363 132
PmeI GTTT'AAAC
Cuts at: 2669 2669
Size: 5365
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3434 3466 4947 4979 3434
Size: 32 1481 32 3820
Fragments arranged by size:
3820 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5365
PsiI TTA'TAA
Cuts at: 2935 2935
Size: 5365
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2451 2483 2451
Size: 32 5333
PstI C_TGCA'G
Cuts at: 830 2269 2717 830
Size: 1439 448 3478
Fragments arranged by size:
3478 1439 448
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5365
SalI G'TCGA_C
Cuts at: 1936 2707 1936
Size: 771 4594
SbfI CC_TGCA'GG
Cuts at: 2717 2717
Size: 5365
ScaI AGT'ACT
Cuts at: 3613 5325 3613
Size: 1712 3653
SfcI C'TryA_G
Cuts at: 826 1045 2265 2314 2426 2713 3848 4529 4720
Size: 219 1220 49 112 287 1135 681 191
Cuts at: 4720 826
Size: 1471
Fragments arranged by size:
1471 1220 1135 681 287 219 191 112
49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5365
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5365
SmaI CCC'GGG
Cuts at: 1872 2692 1872
Size: 820 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3469 4370 4632 820
Size: 197 926 1526 901 262 1553
Fragments arranged by size:
1553 1526 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3156 493
Size: 2663 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5365
SphI G_CATG'C
Cuts at: 1132 2723 1132
Size: 1591 3774
SspI AAT'ATT
Cuts at: 5 52 3289 5
Size: 47 3237 2081
Fragments arranged by size:
3237 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5365
StyI C'CwwG_G
Cuts at: 513 1065 2004 2194 2214 2238 2435 2804 513
Size: 552 939 190 20 24 197 369 3074
Fragments arranged by size:
3074 939 552 369 197 190 24 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3392 3409 3562 3747 4192 325
Size: 1255 1812 17 153 185 445 1498
Fragments arranged by size:
1812 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2332 2488 3611
Size: 274 80 33 51 1443 355 156 1123
Cuts at: 3611 5323 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1123 355 274 156 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5325 5357 5325
Size: 32 5333
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5365
XbaI T'CTAG_A
Cuts at: 2701 2701
Size: 5365
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5365
XmaI C'CCGG_G
Cuts at: 1870 2690 1870
Size: 820 4545
XmnI GAAnn'nnTTC
Cuts at: 2397 2513 3494 2397
Size: 116 981 4268
Fragments arranged by size:
4268 981 116
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3173 276
Size: 53 83 186 936 1639 2468
Fragments arranged by size:
2468 1639 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI