Restriction map for
pFN21AE0182
(Circular) (Six-base) MAPSORT of: pFN21AE0182.seq Check: 8084 from: 1 to: 5383
pFN21AE0182 5383 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3193 278
Size: 53 83 186 936 1657 2468
Fragments arranged by size:
2468 1657 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2726 1937
Size: 789 4594
Acc65I G'GTAC_C
Cuts at: 2704 2704
Size: 5383
AclI AA'CG_TT
Cuts at: 1563 3510 3883 1563
Size: 1947 373 3063
Fragments arranged by size:
3063 1947 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2352 2673 3446 4830 5098 1632
Size: 720 321 773 1384 268 1917
Fragments arranged by size:
1917 1384 773 720 321 268
AfeI AGC'GCT
Cuts at: 2093 2123 2093
Size: 30 5353
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5186
AflIII A'CryG_T
Cuts at: 1203 4282 5259 1203
Size: 3079 977 1327
Fragments arranged by size:
3079 1327 977
AgeI A'CCGG_T
Cuts at: 4214 4214
Size: 5383
AhdI GACnn_n'nnGTC
Cuts at: 4112 4112
Size: 5383
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5383
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5383
AlwNI CAG_nnn'CTG
Cuts at: 2572 2651 3126 4698 2572
Size: 79 475 1572 3257
Fragments arranged by size:
3257 1572 475 79
ApaLI G'TGCA_C
Cuts at: 3440 4596 3440
Size: 1156 4227
ApoI r'AATT_y
Cuts at: 1493 2692 2918 3070 5179 1493
Size: 1199 226 152 2109 1697
Fragments arranged by size:
2109 1697 1199 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3937 160
Size: 872 2905 1606
Fragments arranged by size:
2905 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5383
AvaI C'yCGr_G
Cuts at: 1870 1943 2266 2708 1870
Size: 73 323 442 4545
Fragments arranged by size:
4545 442 323 73
BamHI G'GATC_C
Cuts at: 2713 3080 2713
Size: 367 5016
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2156 2704 4159 618
Size: 325 217 627 369 548 1455 1842
Fragments arranged by size:
1842 1455 627 548 369 325 217
BanII G_rGCy'C
Cuts at: 1391 2181 2348 2702 1391
Size: 790 167 354 4072
Fragments arranged by size:
4072 790 354 167
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2253 2369 928
Size: 971 354 116 3942
Fragments arranged by size:
3942 971 354 116
BbvCI CC'TCA_GC
Cuts at: 2583 2583
Size: 5383
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2470 2504 3574 3608 1903
Size: 34 533 34 1070 34 3678
Fragments arranged by size:
3678 1070 533 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3275 4485 3275
Size: 1210 4173
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5383
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2646 2655 3994
Size: 107 122 71 631 605 974 9 1339
Cuts at: 3994 136
Size: 1525
Fragments arranged by size:
1525 1339 974 631 605 122 107 71
9
BglII A'GATC_T
Cuts at: 5378 5378
Size: 5383
BlpI GC'TnA_GC
Cuts at: 2800 2800
Size: 5383
Bme1580I G_kGCm'C
Cuts at: 1790 3444 4600 1790
Size: 1654 1156 2573
Fragments arranged by size:
2573 1654 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5383
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2164 3114 4072
Size: 416 53 83 399 24 747 950 958
Cuts at: 4072 442
Size: 1753
Fragments arranged by size:
1753 958 950 747 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5383
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2258 4043 1239
Size: 1019 1785 2579
Fragments arranged by size:
2579 1785 1019
Bpu10I CC'TnA_GC
Cuts at: 2583 2583
Size: 5383
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3508 4373 4671 3508
Size: 865 298 4220
Fragments arranged by size:
4220 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2103 2159 2276 4046 882
Size: 617 604 56 117 1770 2219
Fragments arranged by size:
2219 1770 617 604 117 56
BsaAI yAC'GTr
Cuts at: 493 1204 3174 493
Size: 711 1970 2702
Fragments arranged by size:
2702 1970 711
BsaBI GATnn'nnATC
Cuts at: 5377 5377
Size: 5383
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3190 3572 4207 275
Size: 53 83 186 936 1657 382 635 1451
Fragments arranged by size:
1657 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3816 4214 4488 4635 5046 3816
Size: 398 274 147 411 4153
Fragments arranged by size:
4153 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2156 2186 1178
Size: 30 165 30 753 30 4375
Fragments arranged by size:
4375 753 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2600 1186
Size: 1414 3969
BseYI C'CCAG_C
Cuts at: 4586 5082 4586
Size: 496 4887
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4810
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2105 2833 3594 4622 1540
Size: 275 186 104 728 761 1028 2301
Fragments arranged by size:
2301 1028 761 728 275 186 104
BsiHKAI G_wGCw'C
Cuts at: 3444 3529 4600 3444
Size: 85 1071 4227
Fragments arranged by size:
4227 1071 85
BsmI GAATG_Cn'
Cuts at: 2533 2894 2987 2533
Size: 361 93 4929
Fragments arranged by size:
4929 361 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2348 2702 3444 3529 4600 1391
Size: 399 391 167 354 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 391 354 167 85
BspEI T'CCGG_A
Cuts at: 5046 5046
Size: 5383
BspHI T'CATG_A
Cuts at: 3272 5002 3272
Size: 1730 3653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2121 2738 844
Size: 1277 617 3489
Fragments arranged by size:
3489 1277 617
BsrBI CCG'CTC
Cuts at: 1394 1823 3270 1394
Size: 429 1447 3507
Fragments arranged by size:
3507 1447 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2286 3878 4052 65
Size: 1183 1038 1592 174 1396
Fragments arranged by size:
1592 1396 1183 1038 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2100 4027 4214 1700
Size: 110 290 1927 187 2869
Fragments arranged by size:
2869 1927 290 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5383
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5383
BssSI C'ACGA_G
Cuts at: 3443 4455 3443
Size: 1012 4371
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5383
BstBI TT'CG_AA
Cuts at: 4187 4187
Size: 5383
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5383
BstXI CCAn_nnnn'nTGG
Cuts at: 2053 2341 2467 2053
Size: 288 126 4969
Fragments arranged by size:
4969 288 126
BstYI r'GATC_y
Cuts at: 1972 2713 3080 3465 3482 4934 5020 5032 5378
Size: 741 367 385 17 1452 86 12 346
Cuts at: 5378 1972
Size: 1977
Fragments arranged by size:
1977 1452 741 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2160 513
Size: 552 939 156 3736
Fragments arranged by size:
3736 939 552 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4644
Fragments arranged by size:
4644 683 56
BtsI GCAGTG_nn'
Cuts at: 2281 2899 3693 3713 2281
Size: 618 794 20 3951
Fragments arranged by size:
3951 794 618 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5383
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5348
DraI TTT'AAA
Cuts at: 2687 2687
Size: 5383
DraIII CAC_nnn'GTG
Cuts at: 2512 2512
Size: 5383
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4390 809
Size: 507 3074 1802
Fragments arranged by size:
3074 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2102 2397
Size: 54 1425 50 275 18 210 62 295
Cuts at: 2397 2830 3719 5348 8
Size: 433 889 1629 43
Fragments arranged by size:
1629 1425 889 433 295 275 210 62
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2102 2830 1537
Size: 275 290 728 4090
Fragments arranged by size:
4090 728 290 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3313 1353
Size: 1960 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3970 4344 4490 387
Size: 1064 138 2381 374 146 1280
Fragments arranged by size:
2381 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2258 2352 2673 3446 4043 4830 5098
Size: 393 626 94 321 773 597 787 268
Cuts at: 5098 1239
Size: 1524
Fragments arranged by size:
1524 787 773 626 597 393 321 268
94
EcoO109I rG'GnC_Cy
Cuts at: 2648 2648
Size: 5383
EcoRI G'AATT_C
Cuts at: 2692 3070 2692
Size: 378 5005
FspI TGC'GCA
Cuts at: 3889 3889
Size: 5383
HaeII r_GCGC'y
Cuts at: 1678 2095 2125 4530 1678
Size: 417 30 2405 2531
Fragments arranged by size:
2531 2405 417 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2156 2188 2431 2463 4024
Size: 32 135 32 584 32 243 32 1561
Cuts at: 4024 4056 4098 4130 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1561 584 243 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2727 2973 669
Size: 988 281 789 246 3079
Fragments arranged by size:
3079 988 789 281 246
HindIII A'AGCT_T
Cuts at: 748 2324 748
Size: 1576 3807
HpaI GTT'AAC
Cuts at: 2973 2973
Size: 5383
KpnI G_GTAC'C
Cuts at: 2708 2708
Size: 5383
MfeI C'AATT_G
Cuts at: 2679 2982 2679
Size: 303 5080
MluI A'CGCG_T
Cuts at: 5259 5259
Size: 5383
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2339 2433 4497 4681 1305
Size: 1034 94 2064 184 2007
Fragments arranged by size:
2064 2007 1034 184 94
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2042 2399 10
Size: 54 1425 343 210 357 2994
Fragments arranged by size:
2994 1425 357 343 210 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2033 2339 2390 2393 3341 3700 3859
Size: 648 867 306 51 3 948 359 159
Cuts at: 3859 518
Size: 2042
Fragments arranged by size:
2042 948 867 648 359 306 159 51
3
MspA1I CmG'CkG
Cuts at: 1606 2799 3476 4624 4869 1606
Size: 1193 677 1148 245 2120
Fragments arranged by size:
2120 1193 1148 677 245
NaeI GCC'GGC
Cuts at: 2102 2102
Size: 5383
NcoI C'CATG_G
Cuts at: 513 1065 2004 2160 513
Size: 552 939 156 3736
Fragments arranged by size:
3736 939 552 156
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5383
NgoMIV G'CCGG_C
Cuts at: 2100 2100
Size: 5383
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5383
NotI GC'GGCC_GC
Cuts at: 2830 2830
Size: 5383
NspI r_CATG'y
Cuts at: 1132 1229 2741 4286 1132
Size: 97 1512 1545 2229
Fragments arranged by size:
2229 1545 1512 97
PasI CC'CwG_GG
Cuts at: 1348 2415 1348
Size: 1067 4316
PciI A'CATG_T
Cuts at: 4282 4282
Size: 5383
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5383
PfoI T'CCnGG_A
Cuts at: 2466 2466
Size: 5383
PmeI GTTT'AAAC
Cuts at: 2687 2687
Size: 5383
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3484 4965 4997 3452
Size: 32 1481 32 3838
Fragments arranged by size:
3838 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5383
PsiI TTA'TAA
Cuts at: 2953 2953
Size: 5383
PstI C_TGCA'G
Cuts at: 830 2121 2667 2735 830
Size: 1291 546 68 3478
Fragments arranged by size:
3478 1291 546 68
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5383
SalI G'TCGA_C
Cuts at: 1936 2725 1936
Size: 789 4594
SbfI CC_TGCA'GG
Cuts at: 2667 2735 2667
Size: 68 5315
ScaI AGT'ACT
Cuts at: 3631 5343 3631
Size: 1712 3671
SexAI A'CCwGG_T
Cuts at: 2508 2508
Size: 5383
SfcI C'TryA_G
Cuts at: 826 1045 2117 2663 2731 3866 4547 4738 826
Size: 219 1072 546 68 1135 681 191 1471
Fragments arranged by size:
1471 1135 1072 681 546 219 191 68
SfiI GGCCn_nnn'nGGCC
Cuts at: 2646 2646
Size: 5383
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5383
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5383
SmaI CCC'GGG
Cuts at: 1872 2710 1872
Size: 838 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3487 4388 4650 820
Size: 197 926 1544 901 262 1553
Fragments arranged by size:
1553 1544 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3174 493
Size: 2681 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5383
SphI G_CATG'C
Cuts at: 1132 2741 1132
Size: 1609 3774
SspI AAT'ATT
Cuts at: 5 52 3307 5
Size: 47 3255 2081
Fragments arranged by size:
3255 2081 47
StuI AGG'CCT
Cuts at: 1517 2550 2640 1517
Size: 1033 90 4260
Fragments arranged by size:
4260 1033 90
StyI C'CwwG_G
Cuts at: 513 1065 2004 2070 2160 2822 513
Size: 552 939 66 90 662 3074
Fragments arranged by size:
3074 939 662 552 90 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3410 3427 3580 3765 4210 325
Size: 1255 1830 17 153 185 445 1498
Fragments arranged by size:
1830 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3629 5341 96
Size: 274 80 33 51 1443 1652 1712 138
Fragments arranged by size:
1712 1652 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5343 5375 5343
Size: 32 5351
Tth111I GACn'n_nGTC
Cuts at: 1316 2170 2201 1316
Size: 854 31 4498
Fragments arranged by size:
4498 854 31
XbaI T'CTAG_A
Cuts at: 2719 2719
Size: 5383
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2035 2050 2011
Size: 24 15 5344
Fragments arranged by size:
5344 24 15
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5383
XmaI C'CCGG_G
Cuts at: 1870 2708 1870
Size: 838 4545
XmnI GAAnn'nnTTC
Cuts at: 2601 3512 2601
Size: 911 4472
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3191 276
Size: 53 83 186 936 1657 2468
Fragments arranged by size:
2468 1657 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfoI SrfI SwaI