Restriction map for
pFN21AE0178
(Circular) (Six-base) MAPSORT of: pFN21AE0178.seq Check: 1903 from: 1 to: 5746
pFN21AE0178 5746 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2766 2766
Size: 5746
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3556 278
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2961 3089 1937
Size: 1024 128 4594
Fragments arranged by size:
4594 1024 128
Acc65I G'GTAC_C
Cuts at: 3067 3067
Size: 5746
AclI AA'CG_TT
Cuts at: 1563 3873 4246 1563
Size: 2310 373 3063
Fragments arranged by size:
3063 2310 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2516 2655 3809 5193 5461 1632
Size: 884 139 1154 1384 268 1917
Fragments arranged by size:
1917 1384 1154 884 268 139
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5549
AflIII A'CryG_T
Cuts at: 1203 2349 4645 5622 1203
Size: 1146 2296 977 1327
Fragments arranged by size:
2296 1327 1146 977
AgeI A'CCGG_T
Cuts at: 4577 4577
Size: 5746
AhdI GACnn_n'nnGTC
Cuts at: 4475 4475
Size: 5746
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5746
AlwNI CAG_nnn'CTG
Cuts at: 2054 2131 2813 3489 5061 2054
Size: 77 682 676 1572 2739
Fragments arranged by size:
2739 1572 682 676 77
ApaLI G'TGCA_C
Cuts at: 3803 4959 3803
Size: 1156 4590
ApoI r'AATT_y
Cuts at: 1493 2114 2528 3055 3281 3433 5542 1493
Size: 621 414 527 226 152 2109 1697
Fragments arranged by size:
2109 1697 621 527 414 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2213 4300 160
Size: 872 1181 2087 1606
Fragments arranged by size:
2087 1606 1181 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
AvaI C'yCGr_G
Cuts at: 1870 1943 3071 1870
Size: 73 1128 4545
Fragments arranged by size:
4545 1128 73
BamHI G'GATC_C
Cuts at: 3076 3443 3076
Size: 367 5379
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3067 4522 618
Size: 325 217 627 1280 1455 1842
Fragments arranged by size:
1842 1455 1280 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2287 2457 3065 1391
Size: 896 170 608 4072
Fragments arranged by size:
4072 896 608 170
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2397 2520 2914 928
Size: 971 498 123 394 3760
Fragments arranged by size:
3760 971 498 394 123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3937 3971 1903
Size: 34 2000 34 3678
Fragments arranged by size:
3678 2000 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2178 3638 4848 2178
Size: 1460 1210 3076
Fragments arranged by size:
3076 1460 1210
BclI T'GATC_A
Cuts at: 1548 2553 1548
Size: 1005 4741
BfrBI ATG'CAT
Cuts at: 2833 2833
Size: 5746
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4357 136
Size: 107 122 71 631 605 2685 1525
Fragments arranged by size:
2685 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5741 5741
Size: 5746
BlpI GC'TnA_GC
Cuts at: 3163 3163
Size: 5746
Bme1580I G_kGCm'C
Cuts at: 1790 2485 3807 4963 1790
Size: 695 1322 1156 2573
Fragments arranged by size:
2573 1322 1156 695
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5746
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3477 4435 442
Size: 416 53 83 399 24 2060 958 1753
Fragments arranged by size:
2060 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5746
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2724 2724
Size: 5746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2301 4406 1239
Size: 1062 2105 2579
Fragments arranged by size:
2579 2105 1062
Bpu10I CC'TnA_GC
Cuts at: 2265 2670 2265
Size: 405 5341
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3871 4736 5034 3871
Size: 865 298 4583
Fragments arranged by size:
4583 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2146 2698 4409 882
Size: 617 647 552 1711 2219
Fragments arranged by size:
2219 1711 647 617 552
BsaAI yAC'GTr
Cuts at: 493 1204 3537 493
Size: 711 2333 2702
Fragments arranged by size:
2702 2333 711
BsaBI GATnn'nnATC
Cuts at: 5740 5740
Size: 5746
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2517 3553 3935 4570
Size: 53 83 186 936 984 1036 382 635
Cuts at: 4570 275
Size: 1451
Fragments arranged by size:
1451 1036 984 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4179 4577 4851 4998 5409 4179
Size: 398 274 147 411 4516
Fragments arranged by size:
4516 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2777 2807 1178
Size: 30 165 30 1374 30 4117
Fragments arranged by size:
4117 1374 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2716 1186
Size: 1530 4216
BseYI C'CCAG_C
Cuts at: 4949 5445 4949
Size: 496 5250
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2455 1163
Size: 573 719 4454
Fragments arranged by size:
4454 719 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3196 3957 4985 1540
Size: 275 186 1195 761 1028 2301
Fragments arranged by size:
2301 1195 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2287 2795 3807 3892 4963 2287
Size: 508 1012 85 1071 3070
Fragments arranged by size:
3070 1071 1012 508 85
BsmI GAATG_Cn'
Cuts at: 3257 3350 3257
Size: 93 5653
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2287 2457 2485 2795 3065 3807 3892
Size: 399 497 170 28 310 270 742 85
Cuts at: 3892 4963 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 497 399 310 270 170
85 28
BspEI T'CCGG_A
Cuts at: 5409 5409
Size: 5746
BspHI T'CATG_A
Cuts at: 3635 5365 3635
Size: 1730 4016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2766 3101 844
Size: 1922 335 3489
Fragments arranged by size:
3489 1922 335
BsrBI CCG'CTC
Cuts at: 1394 1823 2674 3633 1394
Size: 429 851 959 3507
Fragments arranged by size:
3507 959 851 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2334 4241 4415 65
Size: 1183 1086 1907 174 1396
Fragments arranged by size:
1907 1396 1183 1086 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2075 4390 4577 1700
Size: 110 265 2315 187 2869
Fragments arranged by size:
2869 2315 265 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5746
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5746
BssSI C'ACGA_G
Cuts at: 3806 4818 3806
Size: 1012 4734
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2839 794
Size: 2045 3701
BstBI TT'CG_AA
Cuts at: 4550 4550
Size: 5746
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5746
BstXI CCAn_nnnn'nTGG
Cuts at: 2087 2087
Size: 5746
BstYI r'GATC_y
Cuts at: 1972 2008 3076 3443 3828 3845 5297 5383 5395
Size: 36 1068 367 385 17 1452 86 12
Cuts at: 5395 5741 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1068 385 367 346 86 36
17 12
Bsu36I CC'TnA_GG
Cuts at: 2613 2613
Size: 5746
BtgI C'CryG_G
Cuts at: 513 1065 2004 2696 513
Size: 552 939 692 3563
Fragments arranged by size:
3563 939 692 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3036 487
Size: 683 56 1810 3197
Fragments arranged by size:
3197 1810 683 56
BtsI GCAGTG_nn'
Cuts at: 2223 3262 4056 4076 2223
Size: 1039 794 20 3893
Fragments arranged by size:
3893 1039 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5711
DraI TTT'AAA
Cuts at: 2784 3050 2784
Size: 266 5480
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4753 809
Size: 507 3437 1802
Fragments arranged by size:
3437 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2077 2925 3193
Size: 54 1425 50 275 18 247 848 268
Cuts at: 3193 4082 5711 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 848 275 268 247 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3193 1537
Size: 275 1381 4090
Fragments arranged by size:
4090 1381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2577 3676 1353
Size: 1224 1099 3423
Fragments arranged by size:
3423 1224 1099
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4333 4707 4853 387
Size: 1064 138 2744 374 146 1280
Fragments arranged by size:
2744 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2285 2285
Size: 5746
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2301 2516 2655 3809 4406 5193 5461
Size: 393 669 215 139 1154 597 787 268
Cuts at: 5461 1239
Size: 1524
Fragments arranged by size:
1524 1154 787 669 597 393 268 215
139
EcoNI CCTnn'n_nnAGG
Cuts at: 2576 2576
Size: 5746
EcoRI G'AATT_C
Cuts at: 3055 3433 3055
Size: 378 5368
EcoRV GAT'ATC
Cuts at: 2950 2950
Size: 5746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2812 2812
Size: 5746
FspI TGC'GCA
Cuts at: 4252 4252
Size: 5746
HaeII r_GCGC'y
Cuts at: 1678 2299 2662 4893 1678
Size: 621 363 2231 2531
Fragments arranged by size:
2531 2231 621 363
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2065 2097 2527 2559 4387
Size: 32 135 32 493 32 430 32 1828
Cuts at: 4387 4419 4461 4493 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1828 493 430 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2020 2962 3090 3336 669
Size: 988 281 82 942 128 246 3079
Fragments arranged by size:
3079 988 942 281 246 128 82
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5746
HpaI GTT'AAC
Cuts at: 3336 3336
Size: 5746
KpnI G_GTAC'C
Cuts at: 3071 3071
Size: 5746
MfeI C'AATT_G
Cuts at: 2106 3345 2106
Size: 1239 4507
MluI A'CGCG_T
Cuts at: 5622 5622
Size: 5746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2556 4860 5044 1305
Size: 1251 2304 184 2007
Fragments arranged by size:
2304 2007 1251 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2927 10
Size: 54 1425 343 1095 2829
Fragments arranged by size:
2829 1425 1095 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2085 2382 3704 4063 4222 518
Size: 648 919 297 1322 359 159 2042
Fragments arranged by size:
2042 1322 919 648 359 297 159
MspA1I CmG'CkG
Cuts at: 1606 2128 2865 3162 3839 4987 5232 1606
Size: 522 737 297 677 1148 245 2120
Fragments arranged by size:
2120 1148 737 677 522 297 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2696 513
Size: 552 939 692 3563
Fragments arranged by size:
3563 939 692 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5746
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5746
NotI GC'GGCC_GC
Cuts at: 3193 3193
Size: 5746
NsiI A_TGCA'T
Cuts at: 2835 2835
Size: 5746
NspI r_CATG'y
Cuts at: 1132 1229 2095 2353 3104 4649 1132
Size: 97 866 258 751 1545 2229
Fragments arranged by size:
2229 1545 866 751 258 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5746
PciI A'CATG_T
Cuts at: 2349 4645 2349
Size: 2296 3450
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2548 2655 2973 3010 1108
Size: 1440 107 318 37 3844
Fragments arranged by size:
3844 1440 318 107 37
PfoI T'CCnGG_A
Cuts at: 2508 2508
Size: 5746
PmeI GTTT'AAAC
Cuts at: 3050 3050
Size: 5746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2663 2695 3815 3847 5328 5360 2663
Size: 32 1120 32 1481 32 3049
Fragments arranged by size:
3049 1481 1120 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5746
PsiI TTA'TAA
Cuts at: 3316 3316
Size: 5746
PstI C_TGCA'G
Cuts at: 830 3098 830
Size: 2268 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5746
PvuII CAG'CTG
Cuts at: 2128 2128
Size: 5746
SacI G_AGCT'C
Cuts at: 2287 2287
Size: 5746
SalI G'TCGA_C
Cuts at: 1936 2960 3088 1936
Size: 1024 128 4594
Fragments arranged by size:
4594 1024 128
SbfI CC_TGCA'GG
Cuts at: 3098 3098
Size: 5746
ScaI AGT'ACT
Cuts at: 3994 5706 3994
Size: 1712 4034
SfcI C'TryA_G
Cuts at: 826 1045 3094 4229 4910 5101 826
Size: 219 2049 1135 681 191 1471
Fragments arranged by size:
2049 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5746
SmaI CCC'GGG
Cuts at: 1872 3073 1872
Size: 1201 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3850 4751 5013 820
Size: 197 926 1907 901 262 1553
Fragments arranged by size:
1907 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3537 493
Size: 3044 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5746
SphI G_CATG'C
Cuts at: 1132 3104 1132
Size: 1972 3774
SspI AAT'ATT
Cuts at: 5 52 3670 5
Size: 47 3618 2081
Fragments arranged by size:
3618 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5746
StyI C'CwwG_G
Cuts at: 513 1065 2004 2205 2522 2696 3185 513
Size: 552 939 201 317 174 489 3074
Fragments arranged by size:
3074 939 552 489 317 201 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2820 2984 3773 3790 3943 4128 4573
Size: 1255 1240 164 789 17 153 185 445
Cuts at: 4573 325
Size: 1498
Fragments arranged by size:
1498 1255 1240 789 445 185 164 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2346 3992 5704
Size: 274 80 33 51 1443 369 1646 1712
Cuts at: 5704 96
Size: 138
Fragments arranged by size:
1712 1646 1443 369 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5706 5738 5706
Size: 32 5714
Tth111I GACn'n_nGTC
Cuts at: 1316 2913 1316
Size: 1597 4149
XbaI T'CTAG_A
Cuts at: 3082 3082
Size: 5746
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5746
XmaI C'CCGG_G
Cuts at: 1870 3071 1870
Size: 1201 4545
XmnI GAAnn'nnTTC
Cuts at: 2586 3875 2586
Size: 1289 4457
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3554 276
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BsmBI BstZ17I DraIII EcoO109I FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI