Restriction map for
pFN21AE0177
(Circular) (Six-base) MAPSORT of: pFN21AE0177.seq Check: 1470 from: 1 to: 5437
pFN21AE0177 5437 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3247 278
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2780 1937
Size: 843 4594
Acc65I G'GTAC_C
Cuts at: 2758 2758
Size: 5437
AclI AA'CG_TT
Cuts at: 1563 3564 3937 1563
Size: 2001 373 3063
Fragments arranged by size:
3063 2001 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2326 3500 4884 5152 1632
Size: 694 1174 1384 268 1917
Fragments arranged by size:
1917 1384 1174 694 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5240
AflIII A'CryG_T
Cuts at: 1203 4336 5313 1203
Size: 3133 977 1327
Fragments arranged by size:
3133 1327 977
AgeI A'CCGG_T
Cuts at: 4268 4268
Size: 5437
AhdI GACnn_n'nnGTC
Cuts at: 4166 4166
Size: 5437
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5437
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2624 1184
Size: 1440 3997
AlwNI CAG_nnn'CTG
Cuts at: 2202 2438 3180 4752 2202
Size: 236 742 1572 2887
Fragments arranged by size:
2887 1572 742 236
ApaLI G'TGCA_C
Cuts at: 3494 4650 3494
Size: 1156 4281
ApoI r'AATT_y
Cuts at: 1493 2746 2972 3124 5233 1493
Size: 1253 226 152 2109 1697
Fragments arranged by size:
2109 1697 1253 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3991 160
Size: 872 2959 1606
Fragments arranged by size:
2959 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
AvaI C'yCGr_G
Cuts at: 1870 1943 2762 1870
Size: 73 819 4545
Fragments arranged by size:
4545 819 73
BamHI G'GATC_C
Cuts at: 2767 3134 2767
Size: 367 5070
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2300 2353 2758 4213 618
Size: 325 217 627 513 53 405 1455 1842
Fragments arranged by size:
1842 1455 627 513 405 325 217 53
BanII G_rGCy'C
Cuts at: 1391 2756 1391
Size: 1365 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4466
BbvCI CC'TCA_GC
Cuts at: 2279 2279
Size: 5437
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2272 2306 3628 3662 1903
Size: 34 335 34 1322 34 3678
Fragments arranged by size:
3678 1322 335 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3329 4539 3329
Size: 1210 4227
BclI T'GATC_A
Cuts at: 1548 2118 1548
Size: 570 4867
BfrBI ATG'CAT
Cuts at: 2656 2656
Size: 5437
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4048 136
Size: 107 122 71 631 605 2376 1525
Fragments arranged by size:
2376 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5432 5432
Size: 5437
BlpI GC'TnA_GC
Cuts at: 2854 2854
Size: 5437
Bme1580I G_kGCm'C
Cuts at: 1790 2182 2305 2358 3498 4654 1790
Size: 392 123 53 1140 1156 2573
Fragments arranged by size:
2573 1156 1140 392 123 53
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5437
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2643 3168 4126
Size: 416 53 83 399 24 1226 525 958
Cuts at: 4126 442
Size: 1753
Fragments arranged by size:
1753 1226 958 525 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2122 2246 4097 1239
Size: 883 124 1851 2579
Fragments arranged by size:
2579 1851 883 124
Bpu10I CC'TnA_GC
Cuts at: 2279 2279
Size: 5437
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3562 4427 4725 3562
Size: 865 298 4274
Fragments arranged by size:
4274 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4100 882
Size: 617 2601 2219
Fragments arranged by size:
2601 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3228 493
Size: 711 2024 2702
Fragments arranged by size:
2702 2024 711
BsaBI GATnn'nnATC
Cuts at: 5431 5431
Size: 5437
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3244 3626 4261 275
Size: 53 83 186 936 1711 382 635 1451
Fragments arranged by size:
1711 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3870 4268 4542 4689 5100 3870
Size: 398 274 147 411 4207
Fragments arranged by size:
4207 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5212
Fragments arranged by size:
5212 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5437
BseYI C'CCAG_C
Cuts at: 2411 2549 4640 5136 2411
Size: 138 2091 496 2712
Fragments arranged by size:
2712 2091 496 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2412 1163
Size: 573 676 4188
Fragments arranged by size:
4188 676 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2887 3648 4676 1540
Size: 275 186 886 761 1028 2301
Fragments arranged by size:
2301 1028 886 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3498 3583 4654 3498
Size: 85 1071 4281
Fragments arranged by size:
4281 1071 85
BsmI GAATG_Cn'
Cuts at: 2948 3041 2948
Size: 93 5344
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2182 2305 2358 2756 3498 3583 4654
Size: 399 392 123 53 398 742 85 1071
Cuts at: 4654 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 398 392 123 85
53
BspEI T'CCGG_A
Cuts at: 5100 5100
Size: 5437
BspHI T'CATG_A
Cuts at: 3326 5056 3326
Size: 1730 3707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2259 2384 2423 2792 844
Size: 1415 125 39 369 3489
Fragments arranged by size:
3489 1415 369 125 39
BsrBI CCG'CTC
Cuts at: 1394 1823 3324 1394
Size: 429 1501 3507
Fragments arranged by size:
3507 1501 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3932 4106 65
Size: 1183 2684 174 1396
Fragments arranged by size:
2684 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4081 4268 1700
Size: 110 2271 187 2869
Fragments arranged by size:
2869 2271 187 110
BsrGI T'GTAC_A
Cuts at: 96 2594 96
Size: 2498 2939
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5437
BssSI C'ACGA_G
Cuts at: 3497 4509 3497
Size: 1012 4425
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2202 794
Size: 1408 4029
BstBI TT'CG_AA
Cuts at: 4241 4241
Size: 5437
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5437
BstXI CCAn_nnnn'nTGG
Cuts at: 2380 2460 2380
Size: 80 5357
BstYI r'GATC_y
Cuts at: 1972 2767 3134 3519 3536 4988 5074 5086 5432
Size: 795 367 385 17 1452 86 12 346
Cuts at: 5432 1972
Size: 1977
Fragments arranged by size:
1977 1452 795 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2295 513
Size: 552 939 291 3655
Fragments arranged by size:
3655 939 552 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4698
Fragments arranged by size:
4698 683 56
BtsI GCAGTG_nn'
Cuts at: 2203 2334 2532 2953 3747 3767 2203
Size: 131 198 421 794 20 3873
Fragments arranged by size:
3873 794 421 198 131 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5437
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5402
DraI TTT'AAA
Cuts at: 2741 2741
Size: 5437
DraIII CAC_nnn'GTG
Cuts at: 2407 2407
Size: 5437
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2255 4444 809
Size: 507 939 2189 1802
Fragments arranged by size:
2189 1802 939 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2370 2884 3773
Size: 54 1425 50 275 18 540 514 889
Cuts at: 3773 5402 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 540 514 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2884 1537
Size: 275 1072 4090
Fragments arranged by size:
4090 1072 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3367 1353
Size: 2014 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4024 4398 4544 387
Size: 1064 138 2435 374 146 1280
Fragments arranged by size:
2435 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2122 2246 2326 3500 4097 4884 5152
Size: 393 490 124 80 1174 597 787 268
Cuts at: 5152 1239
Size: 1524
Fragments arranged by size:
1524 1174 787 597 490 393 268 124
80
EcoO109I rG'GnC_Cy
Cuts at: 2346 2554 2346
Size: 208 5229
EcoRI G'AATT_C
Cuts at: 2746 3124 2746
Size: 378 5059
FspI TGC'GCA
Cuts at: 3943 3943
Size: 5437
HaeII r_GCGC'y
Cuts at: 1678 4584 1678
Size: 2906 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4078 4110 4152 4184 1373
Size: 32 135 32 2506 32 42 32 2626
Fragments arranged by size:
2626 2506 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2239 2781 3027 669
Size: 988 281 301 542 246 3079
Fragments arranged by size:
3079 988 542 301 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5437
HpaI GTT'AAC
Cuts at: 3027 3027
Size: 5437
KpnI G_GTAC'C
Cuts at: 2762 2762
Size: 5437
MfeI C'AATT_G
Cuts at: 3036 3036
Size: 5437
MluI A'CGCG_T
Cuts at: 5313 5313
Size: 5437
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4551 4735 1305
Size: 3246 184 2007
Fragments arranged by size:
3246 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2372 10
Size: 54 1425 343 540 3075
Fragments arranged by size:
3075 1425 540 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3395 3754 3913 518
Size: 648 2229 359 159 2042
Fragments arranged by size:
2229 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2245 2853 3530 4678 4923 1606
Size: 639 608 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 639 608 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2295 513
Size: 552 939 291 3655
Fragments arranged by size:
3655 939 552 291
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5437
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5437
NotI GC'GGCC_GC
Cuts at: 2884 2884
Size: 5437
NsiI A_TGCA'T
Cuts at: 2658 2658
Size: 5437
NspI r_CATG'y
Cuts at: 1132 1229 2287 2601 2795 4340 1132
Size: 97 1058 314 194 1545 2229
Fragments arranged by size:
2229 1545 1058 314 194 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5437
PciI A'CATG_T
Cuts at: 4336 4336
Size: 5437
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2407 1108
Size: 1299 4138
PmeI GTTT'AAAC
Cuts at: 2741 2741
Size: 5437
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 5019 5051 3506
Size: 32 1481 32 3892
Fragments arranged by size:
3892 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2346 2346
Size: 5437
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5437
PsiI TTA'TAA
Cuts at: 2475 3007 2475
Size: 532 4905
PstI C_TGCA'G
Cuts at: 830 2789 830
Size: 1959 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5437
PvuII CAG'CTG
Cuts at: 2245 2245
Size: 5437
SalI G'TCGA_C
Cuts at: 1936 2779 1936
Size: 843 4594
SbfI CC_TGCA'GG
Cuts at: 2789 2789
Size: 5437
ScaI AGT'ACT
Cuts at: 3685 5397 3685
Size: 1712 3725
SexAI A'CCwGG_T
Cuts at: 2403 2403
Size: 5437
SfcI C'TryA_G
Cuts at: 826 1045 2785 3920 4601 4792 826
Size: 219 1740 1135 681 191 1471
Fragments arranged by size:
1740 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5437
SmaI CCC'GGG
Cuts at: 1872 2764 1872
Size: 892 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3541 4442 4704 820
Size: 197 926 1598 901 262 1553
Fragments arranged by size:
1598 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3228 493
Size: 2735 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5437
SphI G_CATG'C
Cuts at: 1132 2287 2795 1132
Size: 1155 508 3774
Fragments arranged by size:
3774 1155 508
SspI AAT'ATT
Cuts at: 5 52 3361 5
Size: 47 3309 2081
Fragments arranged by size:
3309 2081 47
StuI AGG'CCT
Cuts at: 1517 2073 2163 1517
Size: 556 90 4791
Fragments arranged by size:
4791 556 90
StyI C'CwwG_G
Cuts at: 513 1065 2004 2295 2876 513
Size: 552 939 291 581 3074
Fragments arranged by size:
3074 939 581 552 291
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2158 2288 2662 3464 3481 3634 3819
Size: 1255 578 130 374 802 17 153 185
Cuts at: 3819 4264 325
Size: 445 1498
Fragments arranged by size:
1498 1255 802 578 445 374 185 153
130 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2594 2634 3683
Size: 274 80 33 51 1443 617 40 1049
Cuts at: 3683 5395 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1049 617 274 138 80 51
40 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5397 5429 5397
Size: 32 5405
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5437
XbaI T'CTAG_A
Cuts at: 2773 2773
Size: 5437
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2365 2408 2365
Size: 43 5394
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5437
XmaI C'CCGG_G
Cuts at: 1870 2762 1870
Size: 892 4545
XmnI GAAnn'nnTTC
Cuts at: 3566 3566
Size: 5437
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3245 276
Size: 53 83 186 936 1711 2468
Fragments arranged by size:
2468 1711 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI