(Circular) (Six-base) MAPSORT of: pFN21AE0176.seq Check: 3941 from: 1 to: 7036 pFN21AE0176 7036 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4846 278 Size: 53 83 186 936 3310 2468 Fragments arranged by size: 3310 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3171 3697 4071 4310 4379 1937 Size: 1234 526 374 239 69 4594 Fragments arranged by size: 4594 1234 526 374 239 69 Acc65I G'GTAC_C Cuts at: 4357 4357 Size: 7036 AclI AA'CG_TT Cuts at: 1563 5163 5536 1563 Size: 3600 373 3063 Fragments arranged by size: 3600 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2020 3071 3108 5099 6483 6751 1632 Size: 388 1051 37 1991 1384 268 1917 Fragments arranged by size: 1991 1917 1384 1051 388 268 37 AflII C'TTAA_G Cuts at: 820 1017 3149 820 Size: 197 2132 4707 Fragments arranged by size: 4707 2132 197 AflIII A'CryG_T Cuts at: 1203 2312 3946 5935 6912 1203 Size: 1109 1634 1989 977 1327 Fragments arranged by size: 1989 1634 1327 1109 977 AgeI A'CCGG_T Cuts at: 5867 5867 Size: 7036 AhdI GACnn_n'nnGTC Cuts at: 5765 5765 Size: 7036 AleI CACnn'nnGTG Cuts at: 1166 2854 1166 Size: 1688 5348 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4106 1184 Size: 2922 4114 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2286 2318 2713 2745 2286 Size: 32 395 32 6577 Fragments arranged by size: 6577 395 32 32 AlwNI CAG_nnn'CTG Cuts at: 2383 3304 4779 6351 2383 Size: 921 1475 1572 3068 Fragments arranged by size: 3068 1572 1475 921 ApaLI G'TGCA_C Cuts at: 5093 6249 5093 Size: 1156 5880 ApoI r'AATT_y Cuts at: 1493 2264 2387 2586 3554 3602 3820 4280 4345 Size: 771 123 199 968 48 218 460 65 Cuts at: 4345 4571 4723 6832 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 968 771 460 226 218 199 152 123 65 48 AseI AT'TA_AT Cuts at: 160 1032 2580 5590 160 Size: 872 1548 3010 1606 Fragments arranged by size: 3010 1606 1548 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7036 AvaI C'yCGr_G Cuts at: 1870 1943 4001 4189 4361 1870 Size: 73 2058 188 172 4545 Fragments arranged by size: 4545 2058 188 172 73 AvrII C'CTAG_G Cuts at: 2365 2365 Size: 7036 BamHI G'GATC_C Cuts at: 3268 4366 4733 3268 Size: 1098 367 5571 Fragments arranged by size: 5571 1098 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3506 4357 5812 618 Size: 325 217 627 1719 851 1455 1842 Fragments arranged by size: 1842 1719 1455 851 627 325 217 BanII G_rGCy'C Cuts at: 1391 4355 1391 Size: 2964 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4338 928 Size: 971 2439 3626 Fragments arranged by size: 3626 2439 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5227 5261 1903 Size: 34 3290 34 3678 Fragments arranged by size: 3678 3290 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4928 6138 4928 Size: 1210 5826 BclI T'GATC_A Cuts at: 1548 1548 Size: 7036 BfrBI ATG'CAT Cuts at: 2166 2166 Size: 7036 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4023 5647 136 Size: 107 122 71 631 605 2351 1624 1525 Fragments arranged by size: 2351 1624 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2806 2814 7031 2806 Size: 8 4217 2811 Fragments arranged by size: 4217 2811 8 BlpI GC'TnA_GC Cuts at: 3855 4453 3855 Size: 598 6438 Bme1580I G_kGCm'C Cuts at: 1790 5097 6253 1790 Size: 3307 1156 2573 Fragments arranged by size: 3307 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2911 1318 Size: 1593 5443 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4767 5725 442 Size: 416 53 83 399 24 3350 958 1753 Fragments arranged by size: 3350 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7036 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4225 4225 Size: 7036 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2126 2504 2881 3614 4136 5696 1239 Size: 887 378 377 733 522 1560 2579 Fragments arranged by size: 2579 1560 887 733 522 378 377 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2000 3250 5161 6026 6324 2000 Size: 1250 1911 865 298 2712 Fragments arranged by size: 2712 1911 1250 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3630 5699 882 Size: 617 2131 2069 2219 Fragments arranged by size: 2219 2131 2069 617 BsaAI yAC'GTr Cuts at: 493 1204 3947 4827 493 Size: 711 2743 880 2702 Fragments arranged by size: 2743 2702 880 711 BsaBI GATnn'nnATC Cuts at: 7030 7030 Size: 7036 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4843 5225 5860 275 Size: 53 83 186 936 3310 382 635 1451 Fragments arranged by size: 3310 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4198 5469 5867 6141 6288 6699 4198 Size: 1271 398 274 147 411 4535 Fragments arranged by size: 4535 1271 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2118 2148 2713 2743 4034 Size: 30 165 30 715 30 565 30 1291 Cuts at: 4034 4064 1178 Size: 30 4150 Fragments arranged by size: 4150 1291 715 565 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2619 2622 2625 2628 2631 2634 3227 4201 Size: 1433 3 3 3 3 3 593 974 Cuts at: 4201 4204 1186 Size: 3 4018 Fragments arranged by size: 4018 1433 974 593 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2505 3993 4135 6239 6735 2505 Size: 1488 142 2104 496 2806 Fragments arranged by size: 2806 2104 1488 496 142 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3911 4267 1163 Size: 573 2175 356 3932 Fragments arranged by size: 3932 2175 573 356 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4486 5247 6275 1540 Size: 275 186 2485 761 1028 2301 Fragments arranged by size: 2485 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2884 3689 5097 5182 6253 2884 Size: 805 1408 85 1071 3667 Fragments arranged by size: 3667 1408 1071 805 85 BsmI GAATG_Cn' Cuts at: 3143 3154 3768 4547 4640 3143 Size: 11 614 779 93 5539 Fragments arranged by size: 5539 779 614 93 11 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2884 3689 4355 5097 5182 6253 1391 Size: 399 1094 805 666 742 85 1071 2174 Fragments arranged by size: 2174 1094 1071 805 742 666 399 85 BspEI T'CCGG_A Cuts at: 6699 6699 Size: 7036 BspHI T'CATG_A Cuts at: 4925 6655 4925 Size: 1730 5306 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3933 4391 844 Size: 3089 458 3489 Fragments arranged by size: 3489 3089 458 BsrBI CCG'CTC Cuts at: 1394 1823 4923 1394 Size: 429 3100 3507 Fragments arranged by size: 3507 3100 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2166 5531 5705 65 Size: 1183 918 3365 174 1396 Fragments arranged by size: 3365 1396 1183 918 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5680 5867 1700 Size: 110 3870 187 2869 Fragments arranged by size: 3870 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7036 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7036 BssSI C'ACGA_G Cuts at: 5096 6108 5096 Size: 1012 6024 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7036 BstBI TT'CG_AA Cuts at: 5840 5840 Size: 7036 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7036 BstYI r'GATC_y Cuts at: 1972 2806 2814 3268 3492 3847 4366 4733 5118 Size: 834 8 454 224 355 519 367 385 Cuts at: 5118 5135 6587 6673 6685 7031 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 834 519 454 385 367 355 346 224 86 17 12 8 BstZ17I GTA'TAC Cuts at: 3172 3698 3172 Size: 526 6510 Bsu36I CC'TnA_GG Cuts at: 2593 2593 Size: 7036 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6484 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6297 Fragments arranged by size: 6297 683 56 BtsI GCAGTG_nn' Cuts at: 2884 3477 4111 4552 5346 5366 2884 Size: 593 634 441 794 20 4554 Fragments arranged by size: 4554 794 634 593 441 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7036 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7001 DraI TTT'AAA Cuts at: 3724 4340 3724 Size: 616 6420 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6043 809 Size: 507 4727 1802 Fragments arranged by size: 4727 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4483 5372 7001 Size: 54 1425 50 275 18 2653 889 1629 Cuts at: 7001 8 Size: 43 Fragments arranged by size: 2653 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4483 1537 Size: 275 2671 4090 Fragments arranged by size: 4090 2671 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3084 3217 4966 1353 Size: 1731 133 1749 3423 Fragments arranged by size: 3423 1749 1731 133 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5623 5997 6143 387 Size: 1064 138 4034 374 146 1280 Fragments arranged by size: 4034 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2020 2126 2504 2881 3071 3108 3614 Size: 393 388 106 378 377 190 37 506 Cuts at: 3614 4136 5099 5696 6483 6751 1239 Size: 522 963 597 787 268 1524 Fragments arranged by size: 1524 963 787 597 522 506 393 388 378 377 268 190 106 37 EcoNI CCTnn'n_nnAGG Cuts at: 3209 3277 3209 Size: 68 6968 EcoO109I rG'GnC_Cy Cuts at: 2040 2369 2459 2040 Size: 329 90 6617 Fragments arranged by size: 6617 329 90 EcoRI G'AATT_C Cuts at: 2586 4345 4723 2586 Size: 1759 378 4899 Fragments arranged by size: 4899 1759 378 EcoRV GAT'ATC Cuts at: 2754 3335 2754 Size: 581 6455 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3824 3824 Size: 7036 FspI TGC'GCA Cuts at: 5542 5542 Size: 7036 HaeII r_GCGC'y Cuts at: 1678 6183 1678 Size: 4505 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2118 2150 3253 3285 3365 Size: 32 135 32 546 32 1103 32 80 Cuts at: 3365 3397 5677 5709 5751 5783 1373 Size: 32 2280 32 42 32 2626 Fragments arranged by size: 2626 2280 1103 546 135 80 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4072 4380 4626 669 Size: 988 281 2134 308 246 3079 Fragments arranged by size: 3079 2134 988 308 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7036 HpaI GTT'AAC Cuts at: 4626 4626 Size: 7036 KpnI G_GTAC'C Cuts at: 4361 4361 Size: 7036 MfeI C'AATT_G Cuts at: 3578 4635 3578 Size: 1057 5979 MluI A'CGCG_T Cuts at: 6912 6912 Size: 7036 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2420 6150 6334 1305 Size: 1115 3730 184 2007 Fragments arranged by size: 3730 2007 1115 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5214 Fragments arranged by size: 5214 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2780 2854 4994 5353 5512 518 Size: 648 1614 74 2140 359 159 2042 Fragments arranged by size: 2140 2042 1614 648 359 159 74 MspA1I CmG'CkG Cuts at: 1606 3421 3993 4167 4452 5129 6277 6522 1606 Size: 1815 572 174 285 677 1148 245 2120 Fragments arranged by size: 2120 1815 1148 677 572 285 245 174 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6484 NdeI CA'TA_TG Cuts at: 387 387 Size: 7036 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7036 NotI GC'GGCC_GC Cuts at: 4483 4483 Size: 7036 NsiI A_TGCA'T Cuts at: 2168 2168 Size: 7036 NspI r_CATG'y Cuts at: 1132 1229 2316 3178 4394 5939 1132 Size: 97 1087 862 1216 1545 2229 Fragments arranged by size: 2229 1545 1216 1087 862 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7036 PciI A'CATG_T Cuts at: 2312 5935 2312 Size: 3623 3413 PflMI CCAn_nnn'nTGG Cuts at: 1108 4302 1108 Size: 3194 3842 PmeI GTTT'AAAC Cuts at: 4340 4340 Size: 7036 PmlI CAC'GTG Cuts at: 3947 3947 Size: 7036 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2713 2745 5105 5137 6618 6650 2713 Size: 32 2360 32 1481 32 3099 Fragments arranged by size: 3099 2360 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2040 2459 2040 Size: 419 6617 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7036 PsiI TTA'TAA Cuts at: 4606 4606 Size: 7036 PstI C_TGCA'G Cuts at: 830 2919 3442 4388 830 Size: 2089 523 946 3478 Fragments arranged by size: 3478 2089 946 523 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7036 PvuII CAG'CTG Cuts at: 3421 3421 Size: 7036 SalI G'TCGA_C Cuts at: 1936 4070 4378 1936 Size: 2134 308 4594 Fragments arranged by size: 4594 2134 308 SbfI CC_TGCA'GG Cuts at: 4388 4388 Size: 7036 ScaI AGT'ACT Cuts at: 5284 6996 5284 Size: 1712 5324 SfcI C'TryA_G Cuts at: 826 1045 2915 3198 3438 3742 4233 4384 5519 Size: 219 1870 283 240 304 491 151 1135 Cuts at: 5519 6200 6391 826 Size: 681 191 1471 Fragments arranged by size: 1870 1471 1135 681 491 304 283 240 219 191 151 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7036 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7036 SmaI CCC'GGG Cuts at: 1872 4363 1872 Size: 2491 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2015 3149 3229 5140 6041 6303 Size: 197 926 72 1134 80 1911 901 262 Cuts at: 6303 820 Size: 1553 Fragments arranged by size: 1911 1553 1134 926 901 262 197 80 72 SnaBI TAC'GTA Cuts at: 493 4827 493 Size: 4334 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7036 SphI G_CATG'C Cuts at: 1132 4394 1132 Size: 3262 3774 SspI AAT'ATT Cuts at: 5 52 3019 4960 5 Size: 47 2967 1941 2081 Fragments arranged by size: 2967 2081 1941 47 StuI AGG'CCT Cuts at: 1517 4121 1517 Size: 2604 4432 StyI C'CwwG_G Cuts at: 513 1065 2365 3812 4475 513 Size: 552 1300 1447 663 3074 Fragments arranged by size: 3074 1447 1300 663 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2982 3572 4010 5063 5080 5233 5418 Size: 1255 1402 590 438 1053 17 153 185 Cuts at: 5418 5863 325 Size: 445 1498 Fragments arranged by size: 1498 1402 1255 1053 590 445 438 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4152 5282 6994 Size: 274 80 33 51 1443 2175 1130 1712 Cuts at: 6994 96 Size: 138 Fragments arranged by size: 2175 1712 1443 1130 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2749 2781 4034 4066 6996 7028 2749 Size: 32 1253 32 2930 32 2757 Fragments arranged by size: 2930 2757 1253 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7036 XbaI T'CTAG_A Cuts at: 3343 4372 3343 Size: 1029 6007 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2831 2856 2831 Size: 25 7011 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7036 XmaI C'CCGG_G Cuts at: 1870 4361 1870 Size: 2491 4545 XmnI GAAnn'nnTTC Cuts at: 3807 5165 3807 Size: 1358 5678 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4844 276 Size: 53 83 186 936 3310 2468 Fragments arranged by size: 3310 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI