Restriction map for
pFN21AE0161
(Circular) (Six-base) MAPSORT of: pFN21AE0161.seq Check: 5858 from: 1 to: 5500
pFN21AE0161 5500 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2192 2192
Size: 5500
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2430 3310 278
Size: 53 83 186 936 894 880 2468
Fragments arranged by size:
2468 936 894 880 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2843 1937
Size: 906 4594
Acc65I G'GTAC_C
Cuts at: 2821 2821
Size: 5500
AclI AA'CG_TT
Cuts at: 1563 3627 4000 1563
Size: 2064 373 3063
Fragments arranged by size:
3063 2064 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2220 3563 4947 5215 1632
Size: 588 1343 1384 268 1917
Fragments arranged by size:
1917 1384 1343 588 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5303
AflIII A'CryG_T
Cuts at: 1203 4399 5376 1203
Size: 3196 977 1327
Fragments arranged by size:
3196 1327 977
AgeI A'CCGG_T
Cuts at: 4331 4331
Size: 5500
AhdI GACnn_n'nnGTC
Cuts at: 2230 4229 2230
Size: 1999 3501
AleI CACnn'nnGTG
Cuts at: 1166 2540 1166
Size: 1374 4126
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5500
AlwNI CAG_nnn'CTG
Cuts at: 2209 3243 4815 2209
Size: 1034 1572 2894
Fragments arranged by size:
2894 1572 1034
ApaI G_GGCC'C
Cuts at: 2345 2345
Size: 5500
ApaLI G'TGCA_C
Cuts at: 3557 4713 3557
Size: 1156 4344
ApoI r'AATT_y
Cuts at: 1493 2809 3035 3187 5296 1493
Size: 1316 226 152 2109 1697
Fragments arranged by size:
2109 1697 1316 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4054 160
Size: 872 3022 1606
Fragments arranged by size:
3022 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5500
AvaI C'yCGr_G
Cuts at: 1870 1943 2825 1870
Size: 73 882 4545
Fragments arranged by size:
4545 882 73
BamHI G'GATC_C
Cuts at: 2443 2830 3197 2443
Size: 387 367 4746
Fragments arranged by size:
4746 387 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2176 2821 4276 618
Size: 325 217 627 389 645 1455 1842
Fragments arranged by size:
1842 1455 645 627 389 325 217
BanII G_rGCy'C
Cuts at: 1391 2345 2819 1391
Size: 954 474 4072
Fragments arranged by size:
4072 954 474
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2226 2257 928
Size: 971 327 31 4171
Fragments arranged by size:
4171 971 327 31
BbvCI CC'TCA_GC
Cuts at: 2120 2120
Size: 5500
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3691 3725 1903
Size: 34 1754 34 3678
Fragments arranged by size:
3678 1754 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3392 4602 3392
Size: 1210 4290
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5500
BfrBI ATG'CAT
Cuts at: 2499 2499
Size: 5500
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4111 136
Size: 107 122 71 631 605 2439 1525
Fragments arranged by size:
2439 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5495 5495
Size: 5500
BlpI GC'TnA_GC
Cuts at: 2917 2917
Size: 5500
Bme1580I G_kGCm'C
Cuts at: 1790 2181 2345 3561 4717 1790
Size: 391 164 1216 1156 2573
Fragments arranged by size:
2573 1216 1156 391 164
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5500
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3231 4189 442
Size: 416 53 83 399 24 1814 958 1753
Fragments arranged by size:
1814 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5500
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2674 2674
Size: 5500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2091 2644 4160 1239
Size: 852 553 1516 2579
Fragments arranged by size:
2579 1516 852 553
Bpu10I CC'TnA_GC
Cuts at: 2120 2120
Size: 5500
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3625 4490 4788 3625
Size: 865 298 4337
Fragments arranged by size:
4337 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2597 4163 882
Size: 617 1098 1566 2219
Fragments arranged by size:
2219 1566 1098 617
BsaAI yAC'GTr
Cuts at: 493 1204 3291 493
Size: 711 2087 2702
Fragments arranged by size:
2702 2087 711
BsaBI GATnn'nnATC
Cuts at: 5494 5494
Size: 5500
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2427 3307 3689 4324
Size: 53 83 186 936 894 880 382 635
Cuts at: 4324 275
Size: 1451
Fragments arranged by size:
1451 936 894 880 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3933 4331 4605 4752 5163 3933
Size: 398 274 147 411 4270
Fragments arranged by size:
4270 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5275
Fragments arranged by size:
5275 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2218 1186
Size: 1032 4468
BseYI C'CCAG_C
Cuts at: 4703 5199 4703
Size: 496 5004
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4927
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2950 3711 4739 1540
Size: 275 186 949 761 1028 2301
Fragments arranged by size:
2301 1028 949 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3561 3646 4717 3561
Size: 85 1071 4344
Fragments arranged by size:
4344 1071 85
BsmI GAATG_Cn'
Cuts at: 2614 3011 3104 2614
Size: 397 93 5010
Fragments arranged by size:
5010 397 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2345 2819 3561 3646 4717 1391
Size: 399 391 164 474 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 474 399 391 164 85
BspEI T'CCGG_A
Cuts at: 5163 5163
Size: 5500
BspHI T'CATG_A
Cuts at: 3389 5119 3389
Size: 1730 3770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2192 2855 844
Size: 1348 663 3489
Fragments arranged by size:
3489 1348 663
BsrBI CCG'CTC
Cuts at: 1394 1823 3387 1394
Size: 429 1564 3507
Fragments arranged by size:
3507 1564 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3995 4169 65
Size: 1183 2747 174 1396
Fragments arranged by size:
2747 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2606 4144 4331 1700
Size: 110 796 1538 187 2869
Fragments arranged by size:
2869 1538 796 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5500
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5500
BssSI C'ACGA_G
Cuts at: 3560 4572 3560
Size: 1012 4488
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2042 794
Size: 1248 4252
BstBI TT'CG_AA
Cuts at: 4304 4304
Size: 5500
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5500
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5500
BstYI r'GATC_y
Cuts at: 1972 2443 2641 2830 3197 3582 3599 5051 5137
Size: 471 198 189 367 385 17 1452 86
Cuts at: 5137 5149 5495 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 471 385 367 346 198 189
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4948
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4761
Fragments arranged by size:
4761 683 56
BtsI GCAGTG_nn'
Cuts at: 2716 3016 3810 3830 2716
Size: 300 794 20 4386
Fragments arranged by size:
4386 794 300 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5500
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5465
DraI TTT'AAA
Cuts at: 2804 2804
Size: 5500
DraIII CAC_nnn'GTG
Cuts at: 2164 2164
Size: 5500
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2360 4507 809
Size: 507 1044 2147 1802
Fragments arranged by size:
2147 1802 1044 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2286 2758 2947
Size: 54 1425 50 275 18 456 472 189
Cuts at: 2947 3836 5465 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 472 456 275 189 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2947 1537
Size: 275 1135 4090
Fragments arranged by size:
4090 1135 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3430 1353
Size: 2077 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4087 4461 4607 387
Size: 1064 138 2498 374 146 1280
Fragments arranged by size:
2498 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2091 2220 2644 3563 4160 4947 5215
Size: 393 459 129 424 919 597 787 268
Cuts at: 5215 1239
Size: 1524
Fragments arranged by size:
1524 919 787 597 459 424 393 268
129
EcoO109I rG'GnC_Cy
Cuts at: 2341 2341
Size: 5500
EcoRI G'AATT_C
Cuts at: 2809 3187 2809
Size: 378 5122
EcoRV GAT'ATC
Cuts at: 2633 2633
Size: 5500
FspI TGC'GCA
Cuts at: 4006 4006
Size: 5500
HaeII r_GCGC'y
Cuts at: 1678 4647 1678
Size: 2969 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2008 2040 2216 2248 4141
Size: 32 135 32 436 32 176 32 1893
Cuts at: 4141 4173 4215 4247 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1893 436 176 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2101 2844 3090 669
Size: 988 281 163 743 246 3079
Fragments arranged by size:
3079 988 743 281 246 163
HindIII A'AGCT_T
Cuts at: 748 2527 748
Size: 1779 3721
HpaI GTT'AAC
Cuts at: 3090 3090
Size: 5500
KpnI G_GTAC'C
Cuts at: 2825 2825
Size: 5500
MfeI C'AATT_G
Cuts at: 3099 3099
Size: 5500
MluI A'CGCG_T
Cuts at: 5376 5376
Size: 5500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2514 4614 4798 1305
Size: 1209 2100 184 2007
Fragments arranged by size:
2100 2007 1209 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2760 10
Size: 54 1425 343 928 2750
Fragments arranged by size:
2750 1425 928 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2540 3458 3817 3976 518
Size: 648 843 531 918 359 159 2042
Fragments arranged by size:
2042 918 843 648 531 359 159
MspA1I CmG'CkG
Cuts at: 1606 2916 3593 4741 4986 1606
Size: 1310 677 1148 245 2120
Fragments arranged by size:
2120 1310 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4948
NdeI CA'TA_TG
Cuts at: 387 2585 387
Size: 2198 3302
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5500
NotI GC'GGCC_GC
Cuts at: 2947 2947
Size: 5500
NsiI A_TGCA'T
Cuts at: 2501 2501
Size: 5500
NspI r_CATG'y
Cuts at: 1132 1229 2058 2858 4403 1132
Size: 97 829 800 1545 2229
Fragments arranged by size:
2229 1545 829 800 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5500
PciI A'CATG_T
Cuts at: 4399 4399
Size: 5500
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5500
PmeI GTTT'AAAC
Cuts at: 2804 2804
Size: 5500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3569 3601 5082 5114 3569
Size: 32 1481 32 3955
Fragments arranged by size:
3955 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5500
PsiI TTA'TAA
Cuts at: 3070 3070
Size: 5500
PspOMI G'GGCC_C
Cuts at: 2341 2341
Size: 5500
PstI C_TGCA'G
Cuts at: 830 2852 830
Size: 2022 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5500
SalI G'TCGA_C
Cuts at: 1936 2842 1936
Size: 906 4594
SbfI CC_TGCA'GG
Cuts at: 2852 2852
Size: 5500
ScaI AGT'ACT
Cuts at: 3748 5460 3748
Size: 1712 3788
SexAI A'CCwGG_T
Cuts at: 2160 2160
Size: 5500
SfcI C'TryA_G
Cuts at: 826 1045 2367 2848 3983 4664 4855 826
Size: 219 1322 481 1135 681 191 1471
Fragments arranged by size:
1471 1322 1135 681 481 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5500
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5500
SmaI CCC'GGG
Cuts at: 1872 2827 1872
Size: 955 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3604 4505 4767 820
Size: 197 926 1661 901 262 1553
Fragments arranged by size:
1661 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3291 493
Size: 2798 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5500
SphI G_CATG'C
Cuts at: 1132 2858 1132
Size: 1726 3774
SspI AAT'ATT
Cuts at: 5 52 3424 5
Size: 47 3372 2081
Fragments arranged by size:
3372 2081 47
StuI AGG'CCT
Cuts at: 1517 2076 1517
Size: 559 4941
StyI C'CwwG_G
Cuts at: 513 1065 2448 2939 513
Size: 552 1383 491 3074
Fragments arranged by size:
3074 1383 552 491
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3527 3544 3697 3882 4327 325
Size: 1255 1947 17 153 185 445 1498
Fragments arranged by size:
1947 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3746 5458 96
Size: 274 80 33 51 1443 1769 1712 138
Fragments arranged by size:
1769 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5460 5492 5460
Size: 32 5468
Tth111I GACn'n_nGTC
Cuts at: 1316 2096 1316
Size: 780 4720
XbaI T'CTAG_A
Cuts at: 2836 2836
Size: 5500
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2497 2696 2768 2497
Size: 199 72 5229
Fragments arranged by size:
5229 199 72
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5500
XmaI C'CCGG_G
Cuts at: 1870 2825 1870
Size: 955 4545
XmnI GAAnn'nnTTC
Cuts at: 3629 3629
Size: 5500
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2428 3308 276
Size: 53 83 186 936 894 880 2468
Fragments arranged by size:
2468 936 894 880 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI
BstZ17I Bsu36I EcoICRI EcoNI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI