Restriction map for
pFN21AE0156
(Circular) (Six-base) MAPSORT of: pFN21AE0156.seq Check: 4377 from: 1 to: 5488
pFN21AE0156 5488 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3298 278
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2831 1937
Size: 894 4594
Acc65I G'GTAC_C
Cuts at: 2809 2809
Size: 5488
AclI AA'CG_TT
Cuts at: 1563 3615 3988 1563
Size: 2052 373 3063
Fragments arranged by size:
3063 2052 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2751 3551 4935 5203 1632
Size: 1119 800 1384 268 1917
Fragments arranged by size:
1917 1384 1119 800 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5291
AflIII A'CryG_T
Cuts at: 1203 2229 2346 4387 5364 1203
Size: 1026 117 2041 977 1327
Fragments arranged by size:
2041 1327 1026 977 117
AgeI A'CCGG_T
Cuts at: 4319 4319
Size: 5488
AhdI GACnn_n'nnGTC
Cuts at: 4217 4217
Size: 5488
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5488
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5488
AlwNI CAG_nnn'CTG
Cuts at: 3231 4803 3231
Size: 1572 3916
ApaLI G'TGCA_C
Cuts at: 3545 4701 3545
Size: 1156 4332
ApoI r'AATT_y
Cuts at: 1493 2797 3023 3175 5284 1493
Size: 1304 226 152 2109 1697
Fragments arranged by size:
2109 1697 1304 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4042 160
Size: 872 3010 1606
Fragments arranged by size:
3010 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
AvaI C'yCGr_G
Cuts at: 1870 1943 2199 2556 2813 1870
Size: 73 256 357 257 4545
Fragments arranged by size:
4545 357 257 256 73
BamHI G'GATC_C
Cuts at: 2577 2818 3185 2577
Size: 241 367 4880
Fragments arranged by size:
4880 367 241
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2253 2393 2809 4264 618
Size: 325 217 627 466 140 416 1455 1842
Fragments arranged by size:
1842 1455 627 466 416 325 217 140
BanII G_rGCy'C
Cuts at: 1391 2807 1391
Size: 1416 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4517
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3679 3713 1903
Size: 34 1742 34 3678
Fragments arranged by size:
3678 1742 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2735 3380 4590 2735
Size: 645 1210 3633
Fragments arranged by size:
3633 1210 645
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5488
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2222 4099 136
Size: 107 122 71 631 605 550 1877 1525
Fragments arranged by size:
1877 1525 631 605 550 122 107 71
BglII A'GATC_T
Cuts at: 5483 5483
Size: 5488
BlpI GC'TnA_GC
Cuts at: 2905 2905
Size: 5488
Bme1580I G_kGCm'C
Cuts at: 1790 2396 3549 4705 1790
Size: 606 1153 1156 2573
Fragments arranged by size:
2573 1156 1153 606
BmgBI CAC'GTC
Cuts at: 1318 2230 1318
Size: 912 4576
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2371 3219 4177
Size: 416 53 83 399 24 954 848 958
Cuts at: 4177 442
Size: 1753
Fragments arranged by size:
1753 958 954 848 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2003 2547 2793 4148 1239
Size: 764 544 246 1355 2579
Fragments arranged by size:
2579 1355 764 544 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2372 3613 4478 4776 2372
Size: 1241 865 298 3084
Fragments arranged by size:
3084 1241 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4151 882
Size: 617 2652 2219
Fragments arranged by size:
2652 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3279 493
Size: 711 2075 2702
Fragments arranged by size:
2702 2075 711
BsaBI GATnn'nnATC
Cuts at: 5482 5482
Size: 5488
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3295 3677 4312
Size: 53 83 186 936 476 1286 382 635
Cuts at: 4312 275
Size: 1451
Fragments arranged by size:
1451 1286 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3921 4319 4593 4740 5151 3921
Size: 398 274 147 411 4258
Fragments arranged by size:
4258 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5263
Fragments arranged by size:
5263 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5488
BseYI C'CCAG_C
Cuts at: 2167 4691 5187 2167
Size: 2524 496 2468
Fragments arranged by size:
2524 2468 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2291 2327 1163
Size: 573 555 36 4324
Fragments arranged by size:
4324 573 555 36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2077 2938 3699 4727 1540
Size: 275 186 76 861 761 1028 2301
Fragments arranged by size:
2301 1028 861 761 275 186 76
BsiHKAI G_wGCw'C
Cuts at: 3549 3634 4705 3549
Size: 85 1071 4332
Fragments arranged by size:
4332 1071 85
BsmI GAATG_Cn'
Cuts at: 2717 2999 3092 2717
Size: 282 93 5113
Fragments arranged by size:
5113 282 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2016 2147 2016
Size: 131 5357
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2396 2807 3549 3634 4705 1391
Size: 399 606 411 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 606 411 399 85
BspEI T'CCGG_A
Cuts at: 5151 5151
Size: 5488
BspHI T'CATG_A
Cuts at: 3377 5107 3377
Size: 1730 3758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2843 844
Size: 1999 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2034 2067 2081 3375 1394
Size: 429 211 33 14 1294 3507
Fragments arranged by size:
3507 1294 429 211 33 14
BsrDI GCAATG_nn'
Cuts at: 65 1248 2607 3983 4157 65
Size: 1183 1359 1376 174 1396
Fragments arranged by size:
1396 1376 1359 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4132 4319 1700
Size: 110 2322 187 2869
Fragments arranged by size:
2869 2322 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5488
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5488
BssSI C'ACGA_G
Cuts at: 3548 4560 3548
Size: 1012 4476
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5488
BstBI TT'CG_AA
Cuts at: 4292 4292
Size: 5488
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5488
BstYI r'GATC_y
Cuts at: 1972 2577 2818 3185 3570 3587 5039 5125 5137
Size: 605 241 367 385 17 1452 86 12
Cuts at: 5137 5483 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 605 385 367 346 241 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2694 2694
Size: 5488
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3997
Fragments arranged by size:
3997 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4749
Fragments arranged by size:
4749 683 56
BtsI GCAGTG_nn'
Cuts at: 3004 3798 3818 3004
Size: 794 20 4674
Fragments arranged by size:
4674 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5488
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5453
DraI TTT'AAA
Cuts at: 2792 2792
Size: 5488
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4495 809
Size: 507 3179 1802
Fragments arranged by size:
3179 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2074 2097 2935
Size: 54 1425 50 275 18 244 23 838
Cuts at: 2935 3824 5453 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 838 275 244 54 50
43 23 18
EagI C'GGCC_G
Cuts at: 1537 1812 2074 2935 1537
Size: 275 262 861 4090
Fragments arranged by size:
4090 861 275 262
EarI CTCTTCn'nnn_
Cuts at: 1353 3418 1353
Size: 2065 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4075 4449 4595 387
Size: 1064 138 2486 374 146 1280
Fragments arranged by size:
2486 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2003 2547 2751 2793 3551 4148 4935
Size: 393 371 544 204 42 758 597 787
Cuts at: 4935 5203 1239
Size: 268 1524
Fragments arranged by size:
1524 787 758 597 544 393 371 268
204 42
EcoO109I rG'GnC_Cy
Cuts at: 2689 2754 2689
Size: 65 5423
EcoRI G'AATT_C
Cuts at: 2797 3175 2797
Size: 378 5110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 5488
FspI TGC'GCA
Cuts at: 3994 3994
Size: 5488
HaeII r_GCGC'y
Cuts at: 1678 4635 1678
Size: 2957 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2562 2594 4129 4161 4203
Size: 32 135 32 990 32 1535 32 42
Cuts at: 4203 4235 1373
Size: 32 2626
Fragments arranged by size:
2626 1535 990 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2383 2832 3078 669
Size: 988 281 445 449 246 3079
Fragments arranged by size:
3079 988 449 445 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5488
HpaI GTT'AAC
Cuts at: 3078 3078
Size: 5488
KpnI G_GTAC'C
Cuts at: 2813 2813
Size: 5488
MfeI C'AATT_G
Cuts at: 3087 3087
Size: 5488
MluI A'CGCG_T
Cuts at: 5364 5364
Size: 5488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4602 4786 1305
Size: 3297 184 2007
Fragments arranged by size:
3297 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3666
Fragments arranged by size:
3666 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3446 3805 3964 518
Size: 648 2280 359 159 2042
Fragments arranged by size:
2280 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2144 2583 2904 3581 4729 4974 1606
Size: 538 439 321 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 538 439 321 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3997
Fragments arranged by size:
3997 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5488
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5488
NotI GC'GGCC_GC
Cuts at: 2935 2935
Size: 5488
NspI r_CATG'y
Cuts at: 1132 1229 2350 2846 4391 1132
Size: 97 1121 496 1545 2229
Fragments arranged by size:
2229 1545 1121 496 97
PasI CC'CwG_GG
Cuts at: 1348 2600 1348
Size: 1252 4236
PciI A'CATG_T
Cuts at: 2346 4387 2346
Size: 2041 3447
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5488
PmeI GTTT'AAAC
Cuts at: 2792 2792
Size: 5488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3557 3589 5070 5102 3557
Size: 32 1481 32 3943
Fragments arranged by size:
3943 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2689 2754 2689
Size: 65 5423
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5488
PsiI TTA'TAA
Cuts at: 3058 3058
Size: 5488
PstI C_TGCA'G
Cuts at: 830 2419 2840 830
Size: 1589 421 3478
Fragments arranged by size:
3478 1589 421
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5488
SalI G'TCGA_C
Cuts at: 1936 2830 1936
Size: 894 4594
SanDI GG'GwC_CC
Cuts at: 2689 2689
Size: 5488
SbfI CC_TGCA'GG
Cuts at: 2840 2840
Size: 5488
ScaI AGT'ACT
Cuts at: 3736 5448 3736
Size: 1712 3776
SfcI C'TryA_G
Cuts at: 826 1045 2415 2836 3971 4652 4843 826
Size: 219 1370 421 1135 681 191 1471
Fragments arranged by size:
1471 1370 1135 681 421 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5488
SmaI CCC'GGG
Cuts at: 1872 2558 2815 1872
Size: 686 257 4545
Fragments arranged by size:
4545 686 257
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2387 3592 4493 4755 820
Size: 197 926 444 1205 901 262 1553
Fragments arranged by size:
1553 1205 926 901 444 262 197
SnaBI TAC'GTA
Cuts at: 493 3279 493
Size: 2786 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5488
SphI G_CATG'C
Cuts at: 1132 2846 1132
Size: 1714 3774
SspI AAT'ATT
Cuts at: 5 52 3412 5
Size: 47 3360 2081
Fragments arranged by size:
3360 2081 47
StuI AGG'CCT
Cuts at: 1517 2276 1517
Size: 759 4729
StyI C'CwwG_G
Cuts at: 513 1065 2004 2046 2520 2927 513
Size: 552 939 42 474 407 3074
Fragments arranged by size:
3074 939 552 474 407 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2339 2632 3515 3532 3685 3870 4315
Size: 1255 759 293 883 17 153 185 445
Cuts at: 4315 325
Size: 1498
Fragments arranged by size:
1498 1255 883 759 445 293 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3734 5446 96
Size: 274 80 33 51 1443 1757 1712 138
Fragments arranged by size:
1757 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5448 5480 5448
Size: 32 5456
Tth111I GACn'n_nGTC
Cuts at: 1316 2369 1316
Size: 1053 4435
XbaI T'CTAG_A
Cuts at: 2824 2824
Size: 5488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2257 2257
Size: 5488
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5488
XmaI C'CCGG_G
Cuts at: 1870 2556 2813 1870
Size: 686 257 4545
Fragments arranged by size:
4545 686 257
XmnI GAAnn'nnTTC
Cuts at: 3617 3617
Size: 5488
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3296 276
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SapI SexAI SfiI
SfoI SrfI SwaI