Restriction map for
pFN21AE0148
(Circular) (Six-base) MAPSORT of: pFN21AE0148.seq Check: 979 from: 1 to: 5383
pFN21AE0148 5383 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2637 2637
Size: 5383
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3193 278
Size: 53 83 186 936 1657 2468
Fragments arranged by size:
2468 1657 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2726 1937
Size: 789 4594
Acc65I G'GTAC_C
Cuts at: 2704 2704
Size: 5383
AclI AA'CG_TT
Cuts at: 1563 3510 3883 1563
Size: 1947 373 3063
Fragments arranged by size:
3063 1947 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3446 4830 5098 1632
Size: 1814 1384 268 1917
Fragments arranged by size:
1917 1814 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5186
AflIII A'CryG_T
Cuts at: 1203 2589 4282 5259 1203
Size: 1386 1693 977 1327
Fragments arranged by size:
1693 1386 1327 977
AgeI A'CCGG_T
Cuts at: 4214 4214
Size: 5383
AhdI GACnn_n'nnGTC
Cuts at: 2047 4112 2047
Size: 2065 3318
AleI CACnn'nnGTG
Cuts at: 1166 2318 1166
Size: 1152 4231
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5383
AlwNI CAG_nnn'CTG
Cuts at: 3126 4698 3126
Size: 1572 3811
ApaI G_GGCC'C
Cuts at: 2013 2654 2013
Size: 641 4742
ApaLI G'TGCA_C
Cuts at: 3440 4596 3440
Size: 1156 4227
ApoI r'AATT_y
Cuts at: 1493 2692 2918 3070 5179 1493
Size: 1199 226 152 2109 1697
Fragments arranged by size:
2109 1697 1199 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3937 160
Size: 872 2905 1606
Fragments arranged by size:
2905 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5383
AvaI C'yCGr_G
Cuts at: 1870 1943 2358 2708 1870
Size: 73 415 350 4545
Fragments arranged by size:
4545 415 350 73
BamHI G'GATC_C
Cuts at: 2713 3080 2713
Size: 367 5016
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2704 4159 618
Size: 325 217 627 917 1455 1842
Fragments arranged by size:
1842 1455 917 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2034 2654 2702 1391
Size: 622 21 620 48 4072
Fragments arranged by size:
4072 622 620 48 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2400 928
Size: 971 501 3911
Fragments arranged by size:
3911 971 501
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3574 3608 1903
Size: 34 1637 34 3678
Fragments arranged by size:
3678 1637 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3275 4485 3275
Size: 1210 4173
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5383
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3994 136
Size: 107 122 71 631 605 2322 1525
Fragments arranged by size:
2322 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5378 5378
Size: 5383
BlpI GC'TnA_GC
Cuts at: 2800 2800
Size: 5383
Bme1580I G_kGCm'C
Cuts at: 1790 2013 2654 3444 4600 1790
Size: 223 641 790 1156 2573
Fragments arranged by size:
2573 1156 790 641 223
BmgBI CAC'GTC
Cuts at: 1318 2044 1318
Size: 726 4657
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3114 4072 442
Size: 416 53 83 399 24 1697 958 1753
Fragments arranged by size:
1753 1697 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5383
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2154 2219 2366 2646 4043 1239
Size: 915 65 147 280 1397 2579
Fragments arranged by size:
2579 1397 915 280 147 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2492 3508 4373 4671 2492
Size: 1016 865 298 3204
Fragments arranged by size:
3204 1016 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2507 4046 882
Size: 617 1008 1539 2219
Fragments arranged by size:
2219 1539 1008 617
BsaAI yAC'GTr
Cuts at: 493 1204 3174 493
Size: 711 1970 2702
Fragments arranged by size:
2702 1970 711
BsaBI GATnn'nnATC
Cuts at: 2243 5377 2243
Size: 3134 2249
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3190 3572 4207 275
Size: 53 83 186 936 1657 382 635 1451
Fragments arranged by size:
1657 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2607 3816 4214 4488 4635 5046 2607
Size: 1209 398 274 147 411 2944
Fragments arranged by size:
2944 1209 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2217 2247 2339 2369 1178
Size: 30 165 30 814 30 92 30 4192
Fragments arranged by size:
4192 814 165 92 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5383
BseYI C'CCAG_C
Cuts at: 2065 2126 2632 4586 5082 2065
Size: 61 506 1954 496 2366
Fragments arranged by size:
2366 1954 506 496 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4810
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2833 3594 4622 1540
Size: 275 186 832 761 1028 2301
Fragments arranged by size:
2301 1028 832 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2034 3444 3529 4600 2034
Size: 1410 85 1071 2817
Fragments arranged by size:
2817 1410 1071 85
BsmI GAATG_Cn'
Cuts at: 2677 2894 2987 2677
Size: 217 93 5073
Fragments arranged by size:
5073 217 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2034 2654 2702 3444 3529 4600
Size: 399 223 21 620 48 742 85 1071
Cuts at: 4600 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 620 399 223 85 48
21
BspEI T'CCGG_A
Cuts at: 5046 5046
Size: 5383
BspHI T'CATG_A
Cuts at: 2145 3272 5002 2145
Size: 1127 1730 2526
Fragments arranged by size:
2526 1730 1127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2121 2637 2738 844
Size: 1277 516 101 3489
Fragments arranged by size:
3489 1277 516 101
BsrBI CCG'CTC
Cuts at: 1394 1823 3270 1394
Size: 429 1447 3507
Fragments arranged by size:
3507 1447 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3878 4052 65
Size: 1183 2630 174 1396
Fragments arranged by size:
2630 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2085 4027 4214 1700
Size: 110 275 1942 187 2869
Fragments arranged by size:
2869 1942 275 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5383
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5383
BssSI C'ACGA_G
Cuts at: 3443 4455 3443
Size: 1012 4371
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5383
BstBI TT'CG_AA
Cuts at: 4187 4187
Size: 5383
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5383
BstXI CCAn_nnnn'nTGG
Cuts at: 2563 2576 2563
Size: 13 5370
BstYI r'GATC_y
Cuts at: 1972 2076 2713 3080 3465 3482 4934 5020 5032
Size: 104 637 367 385 17 1452 86 12
Cuts at: 5032 5378 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 637 385 367 346 104 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2025 2562 513
Size: 552 939 21 537 3334
Fragments arranged by size:
3334 939 552 537 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4644
Fragments arranged by size:
4644 683 56
BtsI GCAGTG_nn'
Cuts at: 2899 3693 3713 2899
Size: 794 20 4569
Fragments arranged by size:
4569 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5383
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5348
DraI TTT'AAA
Cuts at: 2687 2687
Size: 5383
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4390 809
Size: 507 3074 1802
Fragments arranged by size:
3074 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2578 2830 3719
Size: 54 1425 50 275 18 748 252 889
Cuts at: 3719 5348 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 748 275 252 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2830 1537
Size: 275 1018 4090
Fragments arranged by size:
4090 1018 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2039 3313 1353
Size: 686 1274 3423
Fragments arranged by size:
3423 1274 686
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3970 4344 4490 387
Size: 1064 138 2381 374 146 1280
Fragments arranged by size:
2381 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2032 2032
Size: 5383
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2154 2219 2366 2646 3446 4043 4830
Size: 393 522 65 147 280 800 597 787
Cuts at: 4830 5098 1239
Size: 268 1524
Fragments arranged by size:
1524 800 787 597 522 393 280 268
147 65
EcoO109I rG'GnC_Cy
Cuts at: 2009 2010 2651 2009
Size: 1 641 4741
Fragments arranged by size:
4741 641 1
EcoRI G'AATT_C
Cuts at: 2692 3070 2692
Size: 378 5005
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2281 2281
Size: 5383
FspI TGC'GCA
Cuts at: 3889 3889
Size: 5383
HaeII r_GCGC'y
Cuts at: 1678 2489 4530 1678
Size: 811 2041 2531
Fragments arranged by size:
2531 2041 811
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 2215 2217 2247
Size: 32 135 32 461 32 150 2 30
Cuts at: 2247 2249 2252 2284 2339 2371 4024 4056 4098
Size: 2 3 32 55 32 1653 32 42
Cuts at: 4098 4130 1373
Size: 32 2626
Fragments arranged by size:
2626 1653 461 150 135 55 42 32
32 32 32 32 32 32 30 3
2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2727 2973 669
Size: 988 281 789 246 3079
Fragments arranged by size:
3079 988 789 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5383
HpaI GTT'AAC
Cuts at: 2973 2973
Size: 5383
KpnI G_GTAC'C
Cuts at: 2708 2708
Size: 5383
MfeI C'AATT_G
Cuts at: 2982 2982
Size: 5383
MluI A'CGCG_T
Cuts at: 5259 5259
Size: 5383
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4497 4681 1305
Size: 3192 184 2007
Fragments arranged by size:
3192 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2580 10
Size: 54 1425 343 748 2813
Fragments arranged by size:
2813 1425 748 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2318 2561 2574 3341 3700 3859 518
Size: 648 1152 243 13 767 359 159 2042
Fragments arranged by size:
2042 1152 767 648 359 243 159 13
MspA1I CmG'CkG
Cuts at: 1606 2799 3476 4624 4869 1606
Size: 1193 677 1148 245 2120
Fragments arranged by size:
2120 1193 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3892
Fragments arranged by size:
3892 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5383
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5383
NotI GC'GGCC_GC
Cuts at: 2830 2830
Size: 5383
NspI r_CATG'y
Cuts at: 1132 1229 2593 2741 4286 1132
Size: 97 1364 148 1545 2229
Fragments arranged by size:
2229 1545 1364 148 97
PasI CC'CwG_GG
Cuts at: 1348 2461 1348
Size: 1113 4270
PciI A'CATG_T
Cuts at: 2589 4282 2589
Size: 1693 3690
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5383
PmeI GTTT'AAAC
Cuts at: 2687 2687
Size: 5383
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3484 4965 4997 3452
Size: 32 1481 32 3838
Fragments arranged by size:
3838 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5383
PsiI TTA'TAA
Cuts at: 2953 2953
Size: 5383
PspOMI G'GGCC_C
Cuts at: 2009 2650 2009
Size: 641 4742
PstI C_TGCA'G
Cuts at: 830 2118 2431 2452 2534 2735 830
Size: 1288 313 21 82 201 3478
Fragments arranged by size:
3478 1288 313 201 82 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5383
SacI G_AGCT'C
Cuts at: 2034 2034
Size: 5383
SalI G'TCGA_C
Cuts at: 1936 2725 1936
Size: 789 4594
SapI GCTCTTCn'nnn_
Cuts at: 2039 2039
Size: 5383
SbfI CC_TGCA'GG
Cuts at: 2735 2735
Size: 5383
ScaI AGT'ACT
Cuts at: 3631 5343 3631
Size: 1712 3671
SfcI C'TryA_G
Cuts at: 826 1045 2114 2427 2448 2530 2731 3866 4547
Size: 219 1069 313 21 82 201 1135 681
Cuts at: 4547 4738 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1069 681 313 219 201 191
82 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5383
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5383
SmaI CCC'GGG
Cuts at: 1872 2360 2710 1872
Size: 488 350 4545
Fragments arranged by size:
4545 488 350
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2507 3487 4388 4650 820
Size: 197 926 564 980 901 262 1553
Fragments arranged by size:
1553 980 926 901 564 262 197
SnaBI TAC'GTA
Cuts at: 493 3174 493
Size: 2681 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5383
SphI G_CATG'C
Cuts at: 1132 2741 1132
Size: 1609 3774
SspI AAT'ATT
Cuts at: 5 52 3307 5
Size: 47 3255 2081
Fragments arranged by size:
3255 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5383
StyI C'CwwG_G
Cuts at: 513 1065 2004 2256 2822 513
Size: 552 939 252 566 3074
Fragments arranged by size:
3074 939 566 552 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3410 3427 3580 3765 4210 325
Size: 1255 1830 17 153 185 445 1498
Fragments arranged by size:
1830 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3629 5341 96
Size: 274 80 33 51 1443 1652 1712 138
Fragments arranged by size:
1712 1652 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5343 5375 5343
Size: 32 5351
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5383
XbaI T'CTAG_A
Cuts at: 2719 2719
Size: 5383
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2594 2594
Size: 5383
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5383
XmaI C'CCGG_G
Cuts at: 1870 2358 2708 1870
Size: 488 350 4545
Fragments arranged by size:
4545 488 350
XmnI GAAnn'nnTTC
Cuts at: 3512 3512
Size: 5383
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3191 276
Size: 53 83 186 936 1657 2468
Fragments arranged by size:
2468 1657 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI