Restriction map for
pFN21AE0143
(Circular) (Six-base) MAPSORT of: pFN21AE0143.seq Check: 7164 from: 1 to: 5635
pFN21AE0143 5635 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3445 278
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2978 1937
Size: 1041 4594
Acc65I G'GTAC_C
Cuts at: 2167 2956 2167
Size: 789 4846
AclI AA'CG_TT
Cuts at: 1563 3762 4135 1563
Size: 2199 373 3063
Fragments arranged by size:
3063 2199 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3698 5082 5350 1632
Size: 2066 1384 268 1917
Fragments arranged by size:
2066 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5438
AflIII A'CryG_T
Cuts at: 1203 4534 5511 1203
Size: 3331 977 1327
Fragments arranged by size:
3331 1327 977
AgeI A'CCGG_T
Cuts at: 4466 4466
Size: 5635
AhdI GACnn_n'nnGTC
Cuts at: 4364 4364
Size: 5635
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5635
AlwNI CAG_nnn'CTG
Cuts at: 3378 4950 3378
Size: 1572 4063
ApaI G_GGCC'C
Cuts at: 2144 2158 2144
Size: 14 5621
ApaLI G'TGCA_C
Cuts at: 3692 4848 3692
Size: 1156 4479
ApoI r'AATT_y
Cuts at: 1493 2305 2944 3170 3322 5431 1493
Size: 812 639 226 152 2109 1697
Fragments arranged by size:
2109 1697 812 639 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2796 4189 160
Size: 872 1764 1393 1606
Fragments arranged by size:
1764 1606 1393 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
AvaI C'yCGr_G
Cuts at: 1870 1943 2098 2960 1870
Size: 73 155 862 4545
Fragments arranged by size:
4545 862 155 73
BamHI G'GATC_C
Cuts at: 2042 2965 3332 2042
Size: 923 367 4345
Fragments arranged by size:
4345 923 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2060 2167 2244 2956
Size: 325 217 627 239 34 107 77 712
Cuts at: 2956 4411 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 712 627 325 239 217 107
77 34
BanII G_rGCy'C
Cuts at: 1391 2144 2158 2625 2954 1391
Size: 753 14 467 329 4072
Fragments arranged by size:
4072 753 467 329 14
BbeI G_GCGC'C
Cuts at: 2064 2064
Size: 5635
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2225 2882 928
Size: 971 326 657 3681
Fragments arranged by size:
3681 971 657 326
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3826 3860 1903
Size: 34 1889 34 3678
Fragments arranged by size:
3678 1889 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3527 4737 3527
Size: 1210 4425
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5635
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2018 4246
Size: 107 122 71 631 605 337 9 2228
Cuts at: 4246 136
Size: 1525
Fragments arranged by size:
2228 1525 631 605 337 122 107 71
9
BglII A'GATC_T
Cuts at: 5630 5630
Size: 5635
BlpI GC'TnA_GC
Cuts at: 3052 3052
Size: 5635
Bme1580I G_kGCm'C
Cuts at: 1790 2144 2158 3696 4852 1790
Size: 354 14 1538 1156 2573
Fragments arranged by size:
2573 1538 1156 354 14
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5635
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3366 4324 442
Size: 416 53 83 399 24 1949 958 1753
Fragments arranged by size:
1949 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2270 4295 1239
Size: 1031 2025 2579
Fragments arranged by size:
2579 2025 1031
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3760 4625 4923 3760
Size: 865 298 4472
Fragments arranged by size:
4472 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4298 882
Size: 617 2799 2219
Fragments arranged by size:
2799 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3426 493
Size: 711 2222 2702
Fragments arranged by size:
2702 2222 711
BsaBI GATnn'nnATC
Cuts at: 5629 5629
Size: 5635
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2061 3442 3824 4459
Size: 53 83 186 936 528 1381 382 635
Cuts at: 4459 275
Size: 1451
Fragments arranged by size:
1451 1381 936 635 528 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4068 4466 4740 4887 5298 4068
Size: 398 274 147 411 4405
Fragments arranged by size:
4405 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5410
Fragments arranged by size:
5410 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2267 2270 1186
Size: 1081 3 4551
Fragments arranged by size:
4551 1081 3
BseYI C'CCAG_C
Cuts at: 2163 4838 5334 2163
Size: 2675 496 2464
Fragments arranged by size:
2675 2464 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5062
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 2178 3085 3846 4874 1540
Size: 275 186 12 165 907 761 1028 2301
Fragments arranged by size:
2301 1028 907 761 275 186 165 12
BsiHKAI G_wGCw'C
Cuts at: 2625 3696 3781 4852 2625
Size: 1071 85 1071 3408
Fragments arranged by size:
3408 1071 1071 85
BsmI GAATG_Cn'
Cuts at: 3146 3239 3146
Size: 93 5542
BsmBI CGTCTCn'nnnn_
Cuts at: 2703 2703
Size: 5635
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2144 2158 2625 2954 3696 3781 4852
Size: 399 354 14 467 329 742 85 1071
Cuts at: 4852 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 467 399 354 329 85
14
BspEI T'CCGG_A
Cuts at: 5298 5298
Size: 5635
BspHI T'CATG_A
Cuts at: 3524 5254 3524
Size: 1730 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2179 2990 844
Size: 1335 811 3489
Fragments arranged by size:
3489 1335 811
BsrBI CCG'CTC
Cuts at: 1394 1823 2138 3522 1394
Size: 429 315 1384 3507
Fragments arranged by size:
3507 1384 429 315
BsrDI GCAATG_nn'
Cuts at: 65 1248 2778 4130 4304 65
Size: 1183 1530 1352 174 1396
Fragments arranged by size:
1530 1396 1352 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2130 4279 4466 1700
Size: 110 320 2149 187 2869
Fragments arranged by size:
2869 2149 320 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5635
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5635
BssSI C'ACGA_G
Cuts at: 3695 4707 3695
Size: 1012 4623
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5635
BstBI TT'CG_AA
Cuts at: 4439 4439
Size: 5635
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5635
BstXI CCAn_nnnn'nTGG
Cuts at: 2668 2668
Size: 5635
BstYI r'GATC_y
Cuts at: 1972 2042 2965 3332 3717 3734 5186 5272 5284
Size: 70 923 367 385 17 1452 86 12
Cuts at: 5284 5630 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 923 385 367 346 86 70
17 12
Bsu36I CC'TnA_GG
Cuts at: 2757 2757
Size: 5635
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 513
Size: 552 939 9 4135
Fragments arranged by size:
4135 939 552 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4896
Fragments arranged by size:
4896 683 56
BtsI GCAGTG_nn'
Cuts at: 2089 2673 3151 3945 3965 2089
Size: 584 478 794 20 3759
Fragments arranged by size:
3759 794 584 478 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5635
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2328 2363 2760 2795 529
Size: 35 1764 35 397 35 3369
Fragments arranged by size:
3369 1764 397 35 35 35
DraI TTT'AAA
Cuts at: 2902 2939 2902
Size: 37 5598
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2886 4642 809
Size: 507 1570 1756 1802
Fragments arranged by size:
1802 1756 1570 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2073 2175
Size: 54 1425 50 275 18 180 63 102
Cuts at: 2175 2613 2832 3082 3971 5600 8
Size: 438 219 250 889 1629 43
Fragments arranged by size:
1629 1425 889 438 275 250 219 180
102 63 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2010 2175 3082 1537
Size: 275 198 165 907 4090
Fragments arranged by size:
4090 907 275 198 165
EarI CTCTTCn'nnn_
Cuts at: 1353 3565 1353
Size: 2212 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4222 4596 4742 387
Size: 1064 138 2633 374 146 1280
Fragments arranged by size:
2633 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2623 2623
Size: 5635
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2270 3698 4295 5082 5350 1239
Size: 393 638 1428 597 787 268 1524
Fragments arranged by size:
1524 1428 787 638 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2252 2568 2615 2252
Size: 316 47 5272
Fragments arranged by size:
5272 316 47
EcoO109I rG'GnC_Cy
Cuts at: 2034 2183 2762 2913 2034
Size: 149 579 151 4756
Fragments arranged by size:
4756 579 151 149
EcoRI G'AATT_C
Cuts at: 2944 3322 2944
Size: 378 5257
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2897 2909 2897
Size: 12 5623
FspI TGC'GCA
Cuts at: 4141 4141
Size: 5635
HaeII r_GCGC'y
Cuts at: 1678 2064 4782 1678
Size: 386 2718 2531
Fragments arranged by size:
2718 2531 386
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4276 4308 4350 4382 1373
Size: 32 135 32 2704 32 42 32 2626
Fragments arranged by size:
2704 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2979 3225 669
Size: 988 281 1041 246 3079
Fragments arranged by size:
3079 1041 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2844 748
Size: 2096 3539
HpaI GTT'AAC
Cuts at: 3225 3225
Size: 5635
KasI G'GCGC_C
Cuts at: 2060 2060
Size: 5635
KpnI G_GTAC'C
Cuts at: 2171 2960 2171
Size: 789 4846
MfeI C'AATT_G
Cuts at: 2442 3234 2442
Size: 792 4843
MluI A'CGCG_T
Cuts at: 5511 5511
Size: 5635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2891 4749 4933 1305
Size: 1586 1858 184 2007
Fragments arranged by size:
2007 1858 1586 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2615 2834 10
Size: 54 1425 343 783 219 2811
Fragments arranged by size:
2811 1425 783 343 219 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2603 3593 3952 4111 518
Size: 648 1437 990 359 159 2042
Fragments arranged by size:
2042 1437 990 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2015 3051 3728 4876 5121 1606
Size: 409 1036 677 1148 245 2120
Fragments arranged by size:
2120 1148 1036 677 409 245
NaeI GCC'GGC
Cuts at: 2132 2132
Size: 5635
NarI GG'CG_CC
Cuts at: 2061 2061
Size: 5635
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4144
Fragments arranged by size:
4144 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5635
NgoMIV G'CCGG_C
Cuts at: 2130 2130
Size: 5635
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5635
NotI GC'GGCC_GC
Cuts at: 2010 3082 2010
Size: 1072 4563
NspI r_CATG'y
Cuts at: 1132 1229 2054 2993 4538 1132
Size: 97 825 939 1545 2229
Fragments arranged by size:
2229 1545 939 825 97
PasI CC'CwG_GG
Cuts at: 1348 2158 2405 1348
Size: 810 247 4578
Fragments arranged by size:
4578 810 247
PciI A'CATG_T
Cuts at: 4534 4534
Size: 5635
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2689 1108
Size: 1581 4054
PmeI GTTT'AAAC
Cuts at: 2939 2939
Size: 5635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3704 3736 5217 5249 3704
Size: 32 1481 32 4090
Fragments arranged by size:
4090 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2183 2762 2183
Size: 579 5056
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5635
PsiI TTA'TAA
Cuts at: 2327 3205 2327
Size: 878 4757
PspOMI G'GGCC_C
Cuts at: 2140 2154 2140
Size: 14 5621
PstI C_TGCA'G
Cuts at: 830 2987 830
Size: 2157 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5635
SacI G_AGCT'C
Cuts at: 2625 2625
Size: 5635
SacII CC_GC'GG
Cuts at: 2016 2016
Size: 5635
SalI G'TCGA_C
Cuts at: 1936 2977 1936
Size: 1041 4594
SanDI GG'GwC_CC
Cuts at: 2183 2183
Size: 5635
SbfI CC_TGCA'GG
Cuts at: 2987 2987
Size: 5635
ScaI AGT'ACT
Cuts at: 3883 5595 3883
Size: 1712 3923
SfcI C'TryA_G
Cuts at: 826 1045 2203 2719 2983 4118 4799 4990 826
Size: 219 1158 516 264 1135 681 191 1471
Fragments arranged by size:
1471 1158 1135 681 516 264 219 191
SfoI GGC'GCC
Cuts at: 2062 2062
Size: 5635
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5635
SmaI CCC'GGG
Cuts at: 1872 2100 2962 1872
Size: 228 862 4545
Fragments arranged by size:
4545 862 228
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3739 4640 4902 820
Size: 197 926 1796 901 262 1553
Fragments arranged by size:
1796 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3426 493
Size: 2933 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5635
SphI G_CATG'C
Cuts at: 1132 2054 2993 1132
Size: 922 939 3774
Fragments arranged by size:
3774 939 922
SspI AAT'ATT
Cuts at: 5 52 3559 5
Size: 47 3507 2081
Fragments arranged by size:
3507 2081 47
StuI AGG'CCT
Cuts at: 1517 2729 1517
Size: 1212 4423
StyI C'CwwG_G
Cuts at: 513 1065 2004 2037 2616 2730 3074 513
Size: 552 939 33 579 114 344 3074
Fragments arranged by size:
3074 939 579 552 344 114 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3662 3679 3832 4017 4462 325
Size: 1255 2082 17 153 185 445 1498
Fragments arranged by size:
2082 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3881 5593 96
Size: 274 80 33 51 1443 1904 1712 138
Fragments arranged by size:
1904 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2259 2291 2401 2433 5595 5627 2259
Size: 32 110 32 3162 32 2267
Fragments arranged by size:
3162 2267 110 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5635
XbaI T'CTAG_A
Cuts at: 2971 2971
Size: 5635
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5635
XmaI C'CCGG_G
Cuts at: 1870 2098 2960 1870
Size: 228 862 4545
Fragments arranged by size:
4545 862 228
XmnI GAAnn'nnTTC
Cuts at: 2889 3764 2889
Size: 875 4760
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3443 276
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SacII SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I DraIII EcoRV FseI FspAI
NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SapI SexAI SfiI SrfI SwaI XcmI