Restriction map for
pFN21AE0140
(Circular) (Six-base) MAPSORT of: pFN21AE0140.seq Check: 8163 from: 1 to: 5560
pFN21AE0140 5560 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3370 278
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2478 2903 1937
Size: 541 425 4594
Fragments arranged by size:
4594 541 425
Acc65I G'GTAC_C
Cuts at: 2881 2881
Size: 5560
AclI AA'CG_TT
Cuts at: 1563 3687 4060 1563
Size: 2124 373 3063
Fragments arranged by size:
3063 2124 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2817 3623 5007 5275 1632
Size: 1185 806 1384 268 1917
Fragments arranged by size:
1917 1384 1185 806 268
AflII C'TTAA_G
Cuts at: 820 1017 2497 820
Size: 197 1480 3883
Fragments arranged by size:
3883 1480 197
AflIII A'CryG_T
Cuts at: 1203 4459 5436 1203
Size: 3256 977 1327
Fragments arranged by size:
3256 1327 977
AgeI A'CCGG_T
Cuts at: 2349 4391 2349
Size: 2042 3518
AhdI GACnn_n'nnGTC
Cuts at: 4289 4289
Size: 5560
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5560
AlwNI CAG_nnn'CTG
Cuts at: 2522 3303 4875 2522
Size: 781 1572 3207
Fragments arranged by size:
3207 1572 781
ApaLI G'TGCA_C
Cuts at: 3617 4773 3617
Size: 1156 4404
ApoI r'AATT_y
Cuts at: 1493 2211 2337 2869 3095 3247 5356 1493
Size: 718 126 532 226 152 2109 1697
Fragments arranged by size:
2109 1697 718 532 226 152 126
AseI AT'TA_AT
Cuts at: 160 1032 4114 160
Size: 872 3082 1606
Fragments arranged by size:
3082 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
AvaI C'yCGr_G
Cuts at: 1870 1943 2064 2885 1870
Size: 73 121 821 4545
Fragments arranged by size:
4545 821 121 73
BamHI G'GATC_C
Cuts at: 2890 3257 2890
Size: 367 5193
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2023 2881 4336 618
Size: 325 217 627 224 12 858 1455 1842
Fragments arranged by size:
1842 1455 858 627 325 224 217 12
BanII G_rGCy'C
Cuts at: 1391 2148 2879 1391
Size: 757 731 4072
Fragments arranged by size:
4072 757 731
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4589
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3751 3785 1903
Size: 34 1814 34 3678
Fragments arranged by size:
3678 1814 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3452 4662 3452
Size: 1210 4350
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5560
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2562 4171 136
Size: 107 122 71 631 605 890 1609 1525
Fragments arranged by size:
1609 1525 890 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5555 5555
Size: 5560
BlpI GC'TnA_GC
Cuts at: 2977 2977
Size: 5560
Bme1580I G_kGCm'C
Cuts at: 1790 2603 3621 4777 1790
Size: 813 1018 1156 2573
Fragments arranged by size:
2573 1156 1018 813
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5560
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3291 4249 442
Size: 416 53 83 399 24 1874 958 1753
Fragments arranged by size:
1874 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4220 1239
Size: 2981 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2129 3685 4550 4848 2129
Size: 1556 865 298 2841
Fragments arranged by size:
2841 1556 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4223 882
Size: 617 2724 2219
Fragments arranged by size:
2724 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3351 493
Size: 711 2147 2702
Fragments arranged by size:
2702 2147 711
BsaBI GATnn'nnATC
Cuts at: 5554 5554
Size: 5560
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3367 3749 4384 275
Size: 53 83 186 936 1834 382 635 1451
Fragments arranged by size:
1834 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2349 3993 4391 4665 4812 5223 2349
Size: 1644 398 274 147 411 2686
Fragments arranged by size:
2686 1644 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5335
Fragments arranged by size:
5335 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 1186
Size: 940 4620
BseYI C'CCAG_C
Cuts at: 2268 2415 2596 4763 5259 2268
Size: 147 181 2167 496 2569
Fragments arranged by size:
2569 2167 496 181 147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2355 1163
Size: 573 619 4368
Fragments arranged by size:
4368 619 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3010 3771 4799 1540
Size: 275 186 1009 761 1028 2301
Fragments arranged by size:
2301 1028 1009 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2739 3621 3706 4777 2739
Size: 882 85 1071 3522
Fragments arranged by size:
3522 1071 882 85
BsmI GAATG_Cn'
Cuts at: 3071 3164 3071
Size: 93 5467
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2603 2739 2879 3621 3706 4777
Size: 399 358 455 136 140 742 85 1071
Cuts at: 4777 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 455 399 358 140 136
85
BspEI T'CCGG_A
Cuts at: 5223 5223
Size: 5560
BspHI T'CATG_A
Cuts at: 3449 5179 3449
Size: 1730 3830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2915 844
Size: 2071 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3447 1394
Size: 429 1624 3507
Fragments arranged by size:
3507 1624 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4055 4229 65
Size: 1183 2807 174 1396
Fragments arranged by size:
2807 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2020 2349 4204 4391 1700
Size: 110 210 329 1855 187 2869
Fragments arranged by size:
2869 1855 329 210 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5560
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5560
BssSI C'ACGA_G
Cuts at: 2388 3620 4632 2388
Size: 1232 1012 3316
Fragments arranged by size:
3316 1232 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2378 794
Size: 1584 3976
BstBI TT'CG_AA
Cuts at: 4364 4364
Size: 5560
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5560
BstYI r'GATC_y
Cuts at: 1972 2890 3257 3642 3659 5111 5197 5209 5555
Size: 918 367 385 17 1452 86 12 346
Cuts at: 5555 1972
Size: 1977
Fragments arranged by size:
1977 1452 918 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2479 2479
Size: 5560
BtgI C'CryG_G
Cuts at: 513 1065 2004 2027 513
Size: 552 939 23 4046
Fragments arranged by size:
4046 939 552 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4821
Fragments arranged by size:
4821 683 56
BtsI GCAGTG_nn'
Cuts at: 2439 2807 3076 3870 3890 2439
Size: 368 269 794 20 4109
Fragments arranged by size:
4109 794 368 269 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5560
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5525
DraI TTT'AAA
Cuts at: 2864 2864
Size: 5560
DraIII CAC_nnn'GTG
Cuts at: 2378 2378
Size: 5560
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4567 809
Size: 507 3251 1802
Fragments arranged by size:
3251 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3007 3896 5525
Size: 54 1425 50 275 18 1177 889 1629
Cuts at: 5525 8
Size: 43
Fragments arranged by size:
1629 1425 1177 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3007 1537
Size: 275 1195 4090
Fragments arranged by size:
4090 1195 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2700 3490 1353
Size: 1347 790 3423
Fragments arranged by size:
3423 1347 790
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4147 4521 4667 387
Size: 1064 138 2558 374 146 1280
Fragments arranged by size:
2558 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2817 3623 4220 5007 5275 1239
Size: 393 1185 806 597 787 268 1524
Fragments arranged by size:
1524 1185 806 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2610 2610
Size: 5560
EcoO109I rG'GnC_Cy
Cuts at: 2221 2221
Size: 5560
EcoRI G'AATT_C
Cuts at: 2869 3247 2869
Size: 378 5182
FspI TGC'GCA
Cuts at: 4066 4066
Size: 5560
HaeII r_GCGC'y
Cuts at: 1678 2416 4707 1678
Size: 738 2291 2531
Fragments arranged by size:
2531 2291 738
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4201 4233 4275 4307 1373
Size: 32 135 32 2629 32 42 32 2626
Fragments arranged by size:
2629 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2904 3150 669
Size: 988 281 966 246 3079
Fragments arranged by size:
3079 988 966 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5560
HpaI GTT'AAC
Cuts at: 3150 3150
Size: 5560
KpnI G_GTAC'C
Cuts at: 2885 2885
Size: 5560
MfeI C'AATT_G
Cuts at: 3159 3159
Size: 5560
MluI A'CGCG_T
Cuts at: 5436 5436
Size: 5560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2402 4674 4858 1305
Size: 1097 2272 184 2007
Fragments arranged by size:
2272 2007 1097 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3738
Fragments arranged by size:
3738 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3518 3877 4036 518
Size: 648 843 1509 359 159 2042
Fragments arranged by size:
2042 1509 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2029 2976 3653 4801 5046 1606
Size: 423 947 677 1148 245 2120
Fragments arranged by size:
2120 1148 947 677 423 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4069
Fragments arranged by size:
4069 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5560
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5560
NotI GC'GGCC_GC
Cuts at: 3007 3007
Size: 5560
NspI r_CATG'y
Cuts at: 1132 1229 2918 4463 1132
Size: 97 1689 1545 2229
Fragments arranged by size:
2229 1689 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5560
PciI A'CATG_T
Cuts at: 4459 4459
Size: 5560
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5560
PfoI T'CCnGG_A
Cuts at: 2178 2178
Size: 5560
PmeI GTTT'AAAC
Cuts at: 2864 2864
Size: 5560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3629 3661 5142 5174 3629
Size: 32 1481 32 4015
Fragments arranged by size:
4015 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2221 2221
Size: 5560
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5560
PsiI TTA'TAA
Cuts at: 3130 3130
Size: 5560
PstI C_TGCA'G
Cuts at: 830 2434 2912 830
Size: 1604 478 3478
Fragments arranged by size:
3478 1604 478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5560
SacII CC_GC'GG
Cuts at: 2030 2030
Size: 5560
SalI G'TCGA_C
Cuts at: 1936 2902 1936
Size: 966 4594
SbfI CC_TGCA'GG
Cuts at: 2912 2912
Size: 5560
ScaI AGT'ACT
Cuts at: 2639 3808 5520 2639
Size: 1169 1712 2679
Fragments arranged by size:
2679 1712 1169
SfcI C'TryA_G
Cuts at: 826 1045 2041 2250 2430 2607 2908 4043 4724
Size: 219 996 209 180 177 301 1135 681
Cuts at: 4724 4915 826
Size: 191 1471
Fragments arranged by size:
1471 1135 996 681 301 219 209 191
180 177
SfiI GGCCn_nnn'nGGCC
Cuts at: 2562 2562
Size: 5560
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5560
SmaI CCC'GGG
Cuts at: 1872 2887 1872
Size: 1015 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2108 2497 3664 4565 4827 820
Size: 197 926 165 389 1167 901 262 1553
Fragments arranged by size:
1553 1167 926 901 389 262 197 165
SnaBI TAC'GTA
Cuts at: 493 3351 493
Size: 2858 2702
SpeI A'CTAG_T
Cuts at: 152 2465 152
Size: 2313 3247
SphI G_CATG'C
Cuts at: 1132 2918 1132
Size: 1786 3774
SspI AAT'ATT
Cuts at: 5 52 3484 5
Size: 47 3432 2081
Fragments arranged by size:
3432 2081 47
StuI AGG'CCT
Cuts at: 1517 2556 2616 1517
Size: 1039 60 4461
Fragments arranged by size:
4461 1039 60
StyI C'CwwG_G
Cuts at: 513 1065 2004 2999 513
Size: 552 939 995 3074
Fragments arranged by size:
3074 995 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2260 3587 3604 3757 3942 4387 325
Size: 1255 680 1327 17 153 185 445 1498
Fragments arranged by size:
1498 1327 1255 680 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2637 3806 5518
Size: 274 80 33 51 1443 660 1169 1712
Cuts at: 5518 96
Size: 138
Fragments arranged by size:
1712 1443 1169 660 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5520 5552 5520
Size: 32 5528
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5560
XbaI T'CTAG_A
Cuts at: 2896 2896
Size: 5560
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5560
XmaI C'CCGG_G
Cuts at: 1870 2885 1870
Size: 1015 4545
XmnI GAAnn'nnTTC
Cuts at: 2830 3689 2830
Size: 859 4701
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3368 276
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacII SalI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SanDI SapI SexAI SfoI SrfI
SwaI XcmI