Restriction map for
pFN21AE0133
(Circular) (Six-base) MAPSORT of: pFN21AE0133.seq Check: 1017 from: 1 to: 5488
pFN21AE0133 5488 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3298 278
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2831 1937
Size: 894 4594
Acc65I G'GTAC_C
Cuts at: 2809 2809
Size: 5488
AclI AA'CG_TT
Cuts at: 1563 3615 3988 1563
Size: 2052 373 3063
Fragments arranged by size:
3063 2052 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2279 3551 4935 5203 1632
Size: 647 1272 1384 268 1917
Fragments arranged by size:
1917 1384 1272 647 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5291
AflIII A'CryG_T
Cuts at: 1203 2265 2783 4387 5364 1203
Size: 1062 518 1604 977 1327
Fragments arranged by size:
1604 1327 1062 977 518
AgeI A'CCGG_T
Cuts at: 4319 4319
Size: 5488
AhdI GACnn_n'nnGTC
Cuts at: 4217 4217
Size: 5488
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5488
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5488
AlwNI CAG_nnn'CTG
Cuts at: 2308 2539 3231 4803 2308
Size: 231 692 1572 2993
Fragments arranged by size:
2993 1572 692 231
ApaLI G'TGCA_C
Cuts at: 2033 3545 4701 2033
Size: 1512 1156 2820
Fragments arranged by size:
2820 1512 1156
ApoI r'AATT_y
Cuts at: 1493 2797 3023 3175 5284 1493
Size: 1304 226 152 2109 1697
Fragments arranged by size:
2109 1697 1304 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4042 160
Size: 872 3010 1606
Fragments arranged by size:
3010 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
AvaI C'yCGr_G
Cuts at: 1870 1943 2813 1870
Size: 73 870 4545
Fragments arranged by size:
4545 870 73
BamHI G'GATC_C
Cuts at: 2818 3185 2818
Size: 367 5121
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2131 2328 2391 2809 4264
Size: 325 217 627 344 197 63 418 1455
Cuts at: 4264 618
Size: 1842
Fragments arranged by size:
1842 1455 627 418 344 325 217 197
63
BanII G_rGCy'C
Cuts at: 1391 2807 1391
Size: 1416 4072
BbeI G_GCGC'C
Cuts at: 2135 2332 2135
Size: 197 5291
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2786 928
Size: 971 887 3630
Fragments arranged by size:
3630 971 887
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3679 3713 1903
Size: 34 1742 34 3678
Fragments arranged by size:
3678 1742 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3380 4590 3380
Size: 1210 4278
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5488
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2159 2676 4099
Size: 107 122 71 631 605 487 517 1423
Cuts at: 4099 136
Size: 1525
Fragments arranged by size:
1525 1423 631 605 517 487 122 107
71
BglII A'GATC_T
Cuts at: 5483 5483
Size: 5488
BlpI GC'TnA_GC
Cuts at: 2905 2905
Size: 5488
Bme1580I G_kGCm'C
Cuts at: 1790 2037 3549 4705 1790
Size: 247 1512 1156 2573
Fragments arranged by size:
2573 1512 1156 247
BmgBI CAC'GTC
Cuts at: 1318 2266 2704 1318
Size: 948 438 4102
Fragments arranged by size:
4102 948 438
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2513 3219 4177
Size: 416 53 83 399 24 1096 706 958
Cuts at: 4177 442
Size: 1753
Fragments arranged by size:
1753 1096 958 706 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5488
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 5488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2693 4148 1239
Size: 1454 1455 2579
Fragments arranged by size:
2579 1455 1454
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2459 2609 3613 4478 4776 2459
Size: 150 1004 865 298 3171
Fragments arranged by size:
3171 1004 865 298 150
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4151 882
Size: 617 2652 2219
Fragments arranged by size:
2652 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2038 2515 3279 493
Size: 711 834 477 764 2702
Fragments arranged by size:
2702 834 764 711 477
BsaBI GATnn'nnATC
Cuts at: 5482 5482
Size: 5488
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2132 2329 2656 3295
Size: 53 83 186 936 599 197 327 639
Cuts at: 3295 3677 4312 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 639 635 599 382 327 197
186 83 53
BsaWI w'CCGG_w
Cuts at: 3921 4319 4593 4740 5151 3921
Size: 398 274 147 411 4258
Fragments arranged by size:
4258 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5263
Fragments arranged by size:
5263 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5488
BseYI C'CCAG_C
Cuts at: 2203 2242 4691 5187 2203
Size: 39 2449 496 2504
Fragments arranged by size:
2504 2449 496 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2204 2321 1163
Size: 573 468 117 4330
Fragments arranged by size:
4330 573 468 117
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2938 3699 4727 1540
Size: 275 186 937 761 1028 2301
Fragments arranged by size:
2301 1028 937 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2037 2121 2512 2601 2709 3549 3634 4705 2037
Size: 84 391 89 108 840 85 1071 2820
Fragments arranged by size:
2820 1071 840 391 108 89 85 84
BsmI GAATG_Cn'
Cuts at: 2999 3092 2999
Size: 93 5395
BsmBI CGTCTCn'nnnn_
Cuts at: 2452 2663 2452
Size: 211 5277
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2121 2512 2601 2709 2807 3549
Size: 399 247 84 391 89 108 98 742
Cuts at: 3549 3634 4705 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 391 247 108 98
89 85 84
BspEI T'CCGG_A
Cuts at: 5151 5151
Size: 5488
BspHI T'CATG_A
Cuts at: 3377 5107 3377
Size: 1730 3758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2843 844
Size: 1999 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3375 1394
Size: 429 1552 3507
Fragments arranged by size:
3507 1552 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3983 4157 65
Size: 1183 2735 174 1396
Fragments arranged by size:
2735 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2048 2141 4132 4319 1700
Size: 110 238 93 1991 187 2869
Fragments arranged by size:
2869 1991 238 187 110 93
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5488
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5488
BssSI C'ACGA_G
Cuts at: 2180 3548 4560 2180
Size: 1368 1012 3108
Fragments arranged by size:
3108 1368 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2308 2507 2539 794
Size: 1514 199 32 3743
Fragments arranged by size:
3743 1514 199 32
BstBI TT'CG_AA
Cuts at: 4292 4292
Size: 5488
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5488
BstXI CCAn_nnnn'nTGG
Cuts at: 2771 2771
Size: 5488
BstYI r'GATC_y
Cuts at: 1972 2818 3185 3570 3587 5039 5125 5137 5483
Size: 846 367 385 17 1452 86 12 346
Cuts at: 5483 1972
Size: 1977
Fragments arranged by size:
1977 1452 846 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2043 2064 2136 2277 2337 2382 2407
Size: 552 978 21 72 141 60 45 25
Cuts at: 2407 513
Size: 3594
Fragments arranged by size:
3594 978 552 141 72 60 45 25
21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2249 487
Size: 683 56 1023 3726
Fragments arranged by size:
3726 1023 683 56
BtsI GCAGTG_nn'
Cuts at: 2309 2330 2360 3004 3798 3818 2309
Size: 21 30 644 794 20 3979
Fragments arranged by size:
3979 794 644 30 21 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5488
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5453
DraI TTT'AAA
Cuts at: 2792 2792
Size: 5488
DraIII CAC_nnn'GTG
Cuts at: 2507 2518 2507
Size: 11 5477
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4495 809
Size: 507 3179 1802
Fragments arranged by size:
3179 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2046 2139
Size: 54 1425 50 275 18 210 6 93
Cuts at: 2139 2160 2454 2520 2935 3824 5453 8
Size: 21 294 66 415 889 1629 43
Fragments arranged by size:
1629 1425 889 415 294 275 210 93
66 54 50 43 21 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2935 1537
Size: 275 1123 4090
Fragments arranged by size:
4090 1123 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2666 3418 1353
Size: 1313 752 3423
Fragments arranged by size:
3423 1313 752
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4075 4449 4595 387
Size: 1064 138 2486 374 146 1280
Fragments arranged by size:
2486 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2279 2693 3551 4148 4935 5203 1239
Size: 393 647 414 858 597 787 268 1524
Fragments arranged by size:
1524 858 787 647 597 414 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2246 2246
Size: 5488
EcoRI G'AATT_C
Cuts at: 2797 3175 2797
Size: 378 5110
FseI GG_CCGG'CC
Cuts at: 2145 2145
Size: 5488
FspI TGC'GCA
Cuts at: 2543 3994 2543
Size: 1451 4037
HaeII r_GCGC'y
Cuts at: 1678 2135 2332 2540 4635 1678
Size: 457 197 208 2095 2531
Fragments arranged by size:
2531 2095 457 208 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4129 4161 4203 4235 1373
Size: 32 135 32 2557 32 42 32 2626
Fragments arranged by size:
2626 2557 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2832 3078 669
Size: 988 281 894 246 3079
Fragments arranged by size:
3079 988 894 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5488
HpaI GTT'AAC
Cuts at: 3078 3078
Size: 5488
KasI G'GCGC_C
Cuts at: 2131 2328 2131
Size: 197 5291
KpnI G_GTAC'C
Cuts at: 2813 2813
Size: 5488
MfeI C'AATT_G
Cuts at: 3087 3087
Size: 5488
MluI A'CGCG_T
Cuts at: 2783 5364 2783
Size: 2581 2907
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4602 4786 1305
Size: 3297 184 2007
Fragments arranged by size:
3297 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2162 2456 10
Size: 54 1425 343 330 294 3042
Fragments arranged by size:
3042 1425 343 330 294 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3446 3805 3964 518
Size: 648 2280 359 159 2042
Fragments arranged by size:
2280 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2339 2409 2525 2904 3581 4729 4974 1606
Size: 733 70 116 379 677 1148 245 2120
Fragments arranged by size:
2120 1148 733 677 379 245 116 70
NaeI GCC'GGC
Cuts at: 2143 2143
Size: 5488
NarI GG'CG_CC
Cuts at: 2132 2329 2132
Size: 197 5291
NcoI C'CATG_G
Cuts at: 513 1065 2277 513
Size: 552 1212 3724
Fragments arranged by size:
3724 1212 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5488
NgoMIV G'CCGG_C
Cuts at: 2141 2141
Size: 5488
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5488
NotI GC'GGCC_GC
Cuts at: 2935 2935
Size: 5488
NspI r_CATG'y
Cuts at: 1132 1229 2846 4391 1132
Size: 97 1617 1545 2229
Fragments arranged by size:
2229 1617 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2485 1348
Size: 1137 4351
PciI A'CATG_T
Cuts at: 4387 4387
Size: 5488
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5488
PmeI GTTT'AAAC
Cuts at: 2792 2792
Size: 5488
PmlI CAC'GTG
Cuts at: 2038 2515 2038
Size: 477 5011
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3557 3589 5070 5102 3557
Size: 32 1481 32 3943
Fragments arranged by size:
3943 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2246 2246
Size: 5488
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5488
PsiI TTA'TAA
Cuts at: 3058 3058
Size: 5488
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2177 2209 2177
Size: 32 5456
PstI C_TGCA'G
Cuts at: 830 2840 830
Size: 2010 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5488
SacII CC_GC'GG
Cuts at: 2340 2410 2340
Size: 70 5418
SalI G'TCGA_C
Cuts at: 1936 2830 1936
Size: 894 4594
SanDI GG'GwC_CC
Cuts at: 2246 2246
Size: 5488
SbfI CC_TGCA'GG
Cuts at: 2840 2840
Size: 5488
ScaI AGT'ACT
Cuts at: 3736 5448 3736
Size: 1712 3776
SfcI C'TryA_G
Cuts at: 826 1045 2836 3971 4652 4843 826
Size: 219 1791 1135 681 191 1471
Fragments arranged by size:
1791 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2133 2330 2133
Size: 197 5291
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5488
SmaI CCC'GGG
Cuts at: 1872 2815 1872
Size: 943 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2438 2624 3592 4493 4755 820
Size: 197 926 495 186 968 901 262 1553
Fragments arranged by size:
1553 968 926 901 495 262 197 186
SnaBI TAC'GTA
Cuts at: 493 3279 493
Size: 2786 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5488
SphI G_CATG'C
Cuts at: 1132 2846 1132
Size: 1714 3774
SspI AAT'ATT
Cuts at: 5 52 3412 5
Size: 47 3360 2081
Fragments arranged by size:
3360 2081 47
StuI AGG'CCT
Cuts at: 1517 2027 1517
Size: 510 4978
StyI C'CwwG_G
Cuts at: 513 1065 2277 2691 2927 513
Size: 552 1212 414 236 3074
Fragments arranged by size:
3074 1212 552 414 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2495 2561 2769 3515 3532 3685 3870
Size: 1255 915 66 208 746 17 153 185
Cuts at: 3870 4315 325
Size: 445 1498
Fragments arranged by size:
1498 1255 915 746 445 208 185 153
66 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3734 5446 96
Size: 274 80 33 51 1443 1757 1712 138
Fragments arranged by size:
1757 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2672 2704 2770 2802 5448 5480 2672
Size: 32 66 32 2646 32 2680
Fragments arranged by size:
2680 2646 66 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5488
XbaI T'CTAG_A
Cuts at: 2824 2824
Size: 5488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2555 2555
Size: 5488
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5488
XmaI C'CCGG_G
Cuts at: 1870 2813 1870
Size: 943 4545
XmnI GAAnn'nnTTC
Cuts at: 3617 3617
Size: 5488
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3296 276
Size: 53 83 186 936 1762 2468
Fragments arranged by size:
2468 1762 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI SacII SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FspAI NruI NsiI PacI PfoI PspOMI PspXI
PvuII RsrII SacI SapI SexAI SfiI SrfI SwaI