Restriction map for
pFN21AE0132
(Circular) (Six-base) MAPSORT of: pFN21AE0132.seq Check: 5588 from: 1 to: 5443
pFN21AE0132 5443 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3253 278
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2786 1937
Size: 849 4594
Acc65I G'GTAC_C
Cuts at: 2764 2764
Size: 5443
AclI AA'CG_TT
Cuts at: 1563 3570 3943 1563
Size: 2007 373 3063
Fragments arranged by size:
3063 2007 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2576 3506 4890 5158 1632
Size: 944 930 1384 268 1917
Fragments arranged by size:
1917 1384 944 930 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5246
AflIII A'CryG_T
Cuts at: 1203 4342 5319 1203
Size: 3139 977 1327
Fragments arranged by size:
3139 1327 977
AgeI A'CCGG_T
Cuts at: 4274 4274
Size: 5443
AhdI GACnn_n'nnGTC
Cuts at: 4172 4172
Size: 5443
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2037 1184
Size: 853 4590
AlwNI CAG_nnn'CTG
Cuts at: 2632 3186 4758 2632
Size: 554 1572 3317
Fragments arranged by size:
3317 1572 554
ApaLI G'TGCA_C
Cuts at: 3500 4656 3500
Size: 1156 4287
ApoI r'AATT_y
Cuts at: 1493 2211 2752 2978 3130 5239 1493
Size: 718 541 226 152 2109 1697
Fragments arranged by size:
2109 1697 718 541 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3997 160
Size: 872 2965 1606
Fragments arranged by size:
2965 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
AvaI C'yCGr_G
Cuts at: 1870 1943 2261 2768 1870
Size: 73 318 507 4545
Fragments arranged by size:
4545 507 318 73
BamHI G'GATC_C
Cuts at: 2773 3140 2773
Size: 367 5076
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2764 4219 618
Size: 325 217 627 977 1455 1842
Fragments arranged by size:
1842 1455 977 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2762 1391
Size: 1371 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4472
BbvCI CC'TCA_GC
Cuts at: 2523 2523
Size: 5443
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3634 3668 1903
Size: 34 1697 34 3678
Fragments arranged by size:
3678 1697 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3335 4545 3335
Size: 1210 4233
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5443
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4054 136
Size: 107 122 71 631 605 2382 1525
Fragments arranged by size:
2382 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5438 5438
Size: 5443
BlpI GC'TnA_GC
Cuts at: 2860 2860
Size: 5443
Bme1580I G_kGCm'C
Cuts at: 1790 2302 3504 4660 1790
Size: 512 1202 1156 2573
Fragments arranged by size:
2573 1202 1156 512
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5443
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3174 4132 442
Size: 416 53 83 399 24 1757 958 1753
Fragments arranged by size:
1757 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2234 2705 4103 1239
Size: 995 471 1398 2579
Fragments arranged by size:
2579 1398 995 471
Bpu10I CC'TnA_GC
Cuts at: 2523 2523
Size: 5443
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2459 3568 4433 4731 2459
Size: 1109 865 298 3171
Fragments arranged by size:
3171 1109 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4106 882
Size: 617 2607 2219
Fragments arranged by size:
2607 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3234 493
Size: 711 2030 2702
Fragments arranged by size:
2702 2030 711
BsaBI GATnn'nnATC
Cuts at: 5437 5437
Size: 5443
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2356 3250 3632 4267
Size: 53 83 186 936 823 894 382 635
Cuts at: 4267 275
Size: 1451
Fragments arranged by size:
1451 936 894 823 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3876 4274 4548 4695 5106 3876
Size: 398 274 147 411 4213
Fragments arranged by size:
4213 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2475 2505 1178
Size: 30 165 30 1072 30 4116
Fragments arranged by size:
4116 1072 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2474 2702 1186
Size: 1288 228 3927
Fragments arranged by size:
3927 1288 228
BseYI C'CCAG_C
Cuts at: 4646 5142 4646
Size: 496 4947
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4870
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2677 2893 3654 4682 1540
Size: 275 186 676 216 761 1028 2301
Fragments arranged by size:
2301 1028 761 676 275 216 186
BsiHKAI G_wGCw'C
Cuts at: 2277 2694 3504 3589 4660 2277
Size: 417 810 85 1071 3060
Fragments arranged by size:
3060 1071 810 417 85
BsmI GAATG_Cn'
Cuts at: 2073 2524 2954 3047 2073
Size: 451 430 93 4469
Fragments arranged by size:
4469 451 430 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2277 2302 2694 2762 3504 3589 4660
Size: 399 487 25 392 68 742 85 1071
Cuts at: 4660 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 487 399 392 85 68
25
BspEI T'CCGG_A
Cuts at: 5106 5106
Size: 5443
BspHI T'CATG_A
Cuts at: 3332 5062 3332
Size: 1730 3713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2574 2798 844
Size: 1730 224 3489
Fragments arranged by size:
3489 1730 224
BsrBI CCG'CTC
Cuts at: 1394 1823 3330 1394
Size: 429 1507 3507
Fragments arranged by size:
3507 1507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2316 3938 4112 65
Size: 1183 1068 1622 174 1396
Fragments arranged by size:
1622 1396 1183 1068 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4087 4274 1700
Size: 110 2277 187 2869
Fragments arranged by size:
2869 2277 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5443
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5443
BssSI C'ACGA_G
Cuts at: 3503 4515 3503
Size: 1012 4431
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5443
BstBI TT'CG_AA
Cuts at: 4247 4247
Size: 5443
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5443
BstXI CCAn_nnnn'nTGG
Cuts at: 2392 2392
Size: 5443
BstYI r'GATC_y
Cuts at: 1972 2773 3140 3525 3542 4994 5080 5092 5438
Size: 801 367 385 17 1452 86 12 346
Cuts at: 5438 1972
Size: 1977
Fragments arranged by size:
1977 1452 801 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2268 2724 513
Size: 552 1203 456 3232
Fragments arranged by size:
3232 1203 552 456
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4704
Fragments arranged by size:
4704 683 56
BtsI GCAGTG_nn'
Cuts at: 2021 2959 3753 3773 2021
Size: 938 794 20 3691
Fragments arranged by size:
3691 938 794 20
ClaI AT'CG_AT
Cuts at: 1554 2196 1554
Size: 642 4801
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5408
DraI TTT'AAA
Cuts at: 2413 2747 2413
Size: 334 5109
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4450 809
Size: 507 3134 1802
Fragments arranged by size:
3134 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2442 2589 2634
Size: 54 1425 50 275 18 612 147 45
Cuts at: 2634 2890 3779 5408 8
Size: 256 889 1629 43
Fragments arranged by size:
1629 1425 889 612 275 256 147 54
50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2890 1537
Size: 275 1078 4090
Fragments arranged by size:
4090 1078 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2535 2733 3373 1353
Size: 1182 198 640 3423
Fragments arranged by size:
3423 1182 640 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2438 4030 4404 4550 387
Size: 1064 138 849 1592 374 146 1280
Fragments arranged by size:
1592 1280 1064 849 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2576 2705 3506 4103 4890 5158
Size: 393 602 342 129 801 597 787 268
Cuts at: 5158 1239
Size: 1524
Fragments arranged by size:
1524 801 787 602 597 393 342 268
129
EcoNI CCTnn'n_nnAGG
Cuts at: 2663 2663
Size: 5443
EcoO109I rG'GnC_Cy
Cuts at: 2656 2656
Size: 5443
EcoRI G'AATT_C
Cuts at: 2752 3130 2752
Size: 378 5065
FspI TGC'GCA
Cuts at: 3949 3949
Size: 5443
HaeII r_GCGC'y
Cuts at: 1678 4590 1678
Size: 2912 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4084 4116 4158 4190 1373
Size: 32 135 32 2512 32 42 32 2626
Fragments arranged by size:
2626 2512 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2494 2787 3033 669
Size: 988 281 556 293 246 3079
Fragments arranged by size:
3079 988 556 293 281 246
HindIII A'AGCT_T
Cuts at: 748 2288 748
Size: 1540 3903
HpaI GTT'AAC
Cuts at: 3033 3033
Size: 5443
KpnI G_GTAC'C
Cuts at: 2768 2768
Size: 5443
MfeI C'AATT_G
Cuts at: 3042 3042
Size: 5443
MluI A'CGCG_T
Cuts at: 5319 5319
Size: 5443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2487 2514 4557 4741 1305
Size: 1182 27 2043 184 2007
Fragments arranged by size:
2043 2007 1182 184 27
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2591 2636 10
Size: 54 1425 343 759 45 2817
Fragments arranged by size:
2817 1425 759 343 54 45
MslI CAynn'nnrTG
Cuts at: 518 1166 2180 3401 3760 3919 518
Size: 648 1014 1221 359 159 2042
Fragments arranged by size:
2042 1221 1014 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2859 3536 4684 4929 1606
Size: 1253 677 1148 245 2120
Fragments arranged by size:
2120 1253 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2268 2724 513
Size: 552 1203 456 3232
Fragments arranged by size:
3232 1203 552 456
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5443
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5443
NotI GC'GGCC_GC
Cuts at: 2890 2890
Size: 5443
NspI r_CATG'y
Cuts at: 1132 1229 2801 4346 1132
Size: 97 1572 1545 2229
Fragments arranged by size:
2229 1572 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5443
PciI A'CATG_T
Cuts at: 4342 4342
Size: 5443
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2632 1108
Size: 1524 3919
PmeI GTTT'AAAC
Cuts at: 2747 2747
Size: 5443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3512 3544 5025 5057 3512
Size: 32 1481 32 3898
Fragments arranged by size:
3898 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5443
PsiI TTA'TAA
Cuts at: 3013 3013
Size: 5443
PstI C_TGCA'G
Cuts at: 830 2016 2026 2571 2706 2713 2795 830
Size: 1186 10 545 135 7 82 3478
Fragments arranged by size:
3478 1186 545 135 82 10 7
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5443
SalI G'TCGA_C
Cuts at: 1936 2785 1936
Size: 849 4594
SbfI CC_TGCA'GG
Cuts at: 2795 2795
Size: 5443
ScaI AGT'ACT
Cuts at: 3691 5403 3691
Size: 1712 3731
SexAI A'CCwGG_T
Cuts at: 2625 2625
Size: 5443
SfcI C'TryA_G
Cuts at: 826 1045 2012 2022 2567 2702 2709 2791 3926
Size: 219 967 10 545 135 7 82 1135
Cuts at: 3926 4607 4798 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 967 681 545 219 191 135
82 10 7
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5443
SmaI CCC'GGG
Cuts at: 1872 2263 2770 1872
Size: 391 507 4545
Fragments arranged by size:
4545 507 391
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2474 3547 4448 4710 820
Size: 197 926 531 1073 901 262 1553
Fragments arranged by size:
1553 1073 926 901 531 262 197
SnaBI TAC'GTA
Cuts at: 493 3234 493
Size: 2741 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5443
SphI G_CATG'C
Cuts at: 1132 2801 1132
Size: 1669 3774
SspI AAT'ATT
Cuts at: 5 52 3367 5
Size: 47 3315 2081
Fragments arranged by size:
3315 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5443
StyI C'CwwG_G
Cuts at: 513 1065 2268 2664 2724 2882 513
Size: 552 1203 396 60 158 3074
Fragments arranged by size:
3074 1203 552 396 158 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3470 3487 3640 3825 4270 325
Size: 1255 1890 17 153 185 445 1498
Fragments arranged by size:
1890 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2451 3689 5401
Size: 274 80 33 51 1443 474 1238 1712
Cuts at: 5401 96
Size: 138
Fragments arranged by size:
1712 1443 1238 474 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5403 5435 5403
Size: 32 5411
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5443
XbaI T'CTAG_A
Cuts at: 2779 2779
Size: 5443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2452 2452
Size: 5443
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5443
XmaI C'CCGG_G
Cuts at: 1870 2261 2768 1870
Size: 391 507 4545
Fragments arranged by size:
4545 507 391
XmnI GAAnn'nnTTC
Cuts at: 2108 2291 3572 2108
Size: 183 1281 3979
Fragments arranged by size:
3979 1281 183
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3251 276
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI