Restriction map for
pFN21AE0125
(Circular) (Six-base) MAPSORT of: pFN21AE0125.seq Check: 4423 from: 1 to: 5068
pFN21AE0125 5068 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2878 278
Size: 53 83 186 936 1342 2468
Fragments arranged by size:
2468 1342 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2340 2411 1937
Size: 403 71 4594
Fragments arranged by size:
4594 403 71
Acc65I G'GTAC_C
Cuts at: 2389 2389
Size: 5068
AclI AA'CG_TT
Cuts at: 1563 3195 3568 1563
Size: 1632 373 3063
Fragments arranged by size:
3063 1632 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3131 4515 4783 1632
Size: 1499 1384 268 1917
Fragments arranged by size:
1917 1499 1384 268
AfeI AGC'GCT
Cuts at: 2114 2114
Size: 5068
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4871
AflIII A'CryG_T
Cuts at: 1203 3967 4944 1203
Size: 2764 977 1327
Fragments arranged by size:
2764 1327 977
AgeI A'CCGG_T
Cuts at: 3899 3899
Size: 5068
AhdI GACnn_n'nnGTC
Cuts at: 3797 3797
Size: 5068
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5068
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5068
AlwNI CAG_nnn'CTG
Cuts at: 2811 4383 2811
Size: 1572 3496
ApaLI G'TGCA_C
Cuts at: 3125 4281 3125
Size: 1156 3912
ApoI r'AATT_y
Cuts at: 1493 2377 2603 2755 4864 1493
Size: 884 226 152 2109 1697
Fragments arranged by size:
2109 1697 884 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3622 160
Size: 872 2590 1606
Fragments arranged by size:
2590 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5068
AvaI C'yCGr_G
Cuts at: 1870 1943 2393 1870
Size: 73 450 4545
Fragments arranged by size:
4545 450 73
BamHI G'GATC_C
Cuts at: 2398 2765 2398
Size: 367 4701
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2208 2389 3844 618
Size: 325 217 627 421 181 1455 1842
Fragments arranged by size:
1842 1455 627 421 325 217 181
BanII G_rGCy'C
Cuts at: 1391 2387 1391
Size: 996 4072
BbeI G_GCGC'C
Cuts at: 2212 2212
Size: 5068
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4097
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3259 3293 1903
Size: 34 50 34 1238 34 3678
Fragments arranged by size:
3678 1238 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2960 4170 2960
Size: 1210 3858
BclI T'GATC_A
Cuts at: 1548 2103 1548
Size: 555 4513
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3679 136
Size: 107 122 71 631 605 2007 1525
Fragments arranged by size:
2007 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5063 5063
Size: 5068
BlpI GC'TnA_GC
Cuts at: 2485 2485
Size: 5068
Bme1580I G_kGCm'C
Cuts at: 1790 3129 4285 1790
Size: 1339 1156 2573
Fragments arranged by size:
2573 1339 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5068
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2799 3757 442
Size: 416 53 83 399 24 1382 958 1753
Fragments arranged by size:
1753 1382 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5068
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3728 1239
Size: 2489 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3193 4058 4356 3193
Size: 865 298 3905
Fragments arranged by size:
3905 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3731 882
Size: 617 2232 2219
Fragments arranged by size:
2232 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2859 493
Size: 711 1655 2702
Fragments arranged by size:
2702 1655 711
BsaBI GATnn'nnATC
Cuts at: 5062 5062
Size: 5068
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2209 2875 3257 3892
Size: 53 83 186 936 676 666 382 635
Cuts at: 3892 275
Size: 1451
Fragments arranged by size:
1451 936 676 666 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3501 3899 4173 4320 4731 3501
Size: 398 274 147 411 3838
Fragments arranged by size:
3838 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4843
Fragments arranged by size:
4843 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5068
BseYI C'CCAG_C
Cuts at: 2087 4271 4767 2087
Size: 2184 496 2388
Fragments arranged by size:
2388 2184 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4495
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2050 2518 3279 4307 1540
Size: 275 186 49 468 761 1028 2301
Fragments arranged by size:
2301 1028 761 468 275 186 49
BsiHKAI G_wGCw'C
Cuts at: 3129 3214 4285 3129
Size: 85 1071 3912
Fragments arranged by size:
3912 1071 85
BsmI GAATG_Cn'
Cuts at: 2579 2672 2579
Size: 93 4975
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2387 3129 3214 4285 1391
Size: 399 597 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 597 399 85
BspEI T'CCGG_A
Cuts at: 4731 4731
Size: 5068
BspHI T'CATG_A
Cuts at: 2957 4687 2957
Size: 1730 3338
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2423 844
Size: 1579 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2955 1394
Size: 429 1132 3507
Fragments arranged by size:
3507 1132 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3563 3737 65
Size: 1183 2315 174 1396
Fragments arranged by size:
2315 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2016 3712 3899 1700
Size: 110 206 1696 187 2869
Fragments arranged by size:
2869 1696 206 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5068
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5068
BssSI C'ACGA_G
Cuts at: 3128 4140 3128
Size: 1012 4056
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5068
BstBI TT'CG_AA
Cuts at: 3872 3872
Size: 5068
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5068
BstXI CCAn_nnnn'nTGG
Cuts at: 2161 2161
Size: 5068
BstYI r'GATC_y
Cuts at: 1972 2070 2398 2765 3150 3167 4619 4705 4717
Size: 98 328 367 385 17 1452 86 12
Cuts at: 4717 5063 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 328 98 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4516
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2272 487
Size: 683 56 1046 3283
Fragments arranged by size:
3283 1046 683 56
BtsI GCAGTG_nn'
Cuts at: 2584 3378 3398 2584
Size: 794 20 4254
Fragments arranged by size:
4254 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5068
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5033
DraI TTT'AAA
Cuts at: 2372 2372
Size: 5068
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4075 809
Size: 507 2759 1802
Fragments arranged by size:
2759 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2515 3404 5033
Size: 54 1425 50 275 18 685 889 1629
Cuts at: 5033 8
Size: 43
Fragments arranged by size:
1629 1425 889 685 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2515 1537
Size: 275 703 4090
Fragments arranged by size:
4090 703 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2998 1353
Size: 1645 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2050 3655 4029 4175 387
Size: 1064 138 461 1605 374 146 1280
Fragments arranged by size:
1605 1280 1064 461 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3131 3728 4515 4783 1239
Size: 393 1499 597 787 268 1524
Fragments arranged by size:
1524 1499 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2377 2755 2377
Size: 378 4690
FspI TGC'GCA
Cuts at: 3574 3574
Size: 5068
HaeII r_GCGC'y
Cuts at: 1678 2116 2212 4215 1678
Size: 438 96 2003 2531
Fragments arranged by size:
2531 2003 438 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3709 3741 3783 3815 1373
Size: 32 135 32 2137 32 42 32 2626
Fragments arranged by size:
2626 2137 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2179 2412 2658 669
Size: 988 281 241 233 246 3079
Fragments arranged by size:
3079 988 281 246 241 233
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5068
HpaI GTT'AAC
Cuts at: 2658 2658
Size: 5068
KasI G'GCGC_C
Cuts at: 2208 2208
Size: 5068
KpnI G_GTAC'C
Cuts at: 2393 2393
Size: 5068
MfeI C'AATT_G
Cuts at: 2667 2667
Size: 5068
MluI A'CGCG_T
Cuts at: 4944 4944
Size: 5068
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4182 4366 1305
Size: 2877 184 2007
Fragments arranged by size:
2877 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3246
Fragments arranged by size:
3246 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3026 3385 3544 518
Size: 648 1860 359 159 2042
Fragments arranged by size:
2042 1860 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2032 2052 2134 2221 2484 3161 4309 4554
Size: 426 20 82 87 263 677 1148 245
Cuts at: 4554 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 426 263 245 87 82
20
NarI GG'CG_CC
Cuts at: 2209 2209
Size: 5068
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4516
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5068
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5068
NotI GC'GGCC_GC
Cuts at: 2515 2515
Size: 5068
NspI r_CATG'y
Cuts at: 1132 1229 2426 3971 1132
Size: 97 1197 1545 2229
Fragments arranged by size:
2229 1545 1197 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5068
PciI A'CATG_T
Cuts at: 3967 3967
Size: 5068
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5068
PfoI T'CCnGG_A
Cuts at: 2142 2166 2142
Size: 24 5044
PmeI GTTT'AAAC
Cuts at: 2372 2372
Size: 5068
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3137 3169 4650 4682 3137
Size: 32 1481 32 3523
Fragments arranged by size:
3523 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5068
PsiI TTA'TAA
Cuts at: 2638 2638
Size: 5068
PstI C_TGCA'G
Cuts at: 830 2220 2420 830
Size: 1390 200 3478
Fragments arranged by size:
3478 1390 200
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5068
PvuII CAG'CTG
Cuts at: 2134 2221 2134
Size: 87 4981
SalI G'TCGA_C
Cuts at: 1936 2410 1936
Size: 474 4594
SbfI CC_TGCA'GG
Cuts at: 2420 2420
Size: 5068
ScaI AGT'ACT
Cuts at: 3316 5028 3316
Size: 1712 3356
SfcI C'TryA_G
Cuts at: 826 1045 2216 2416 3551 4232 4423 826
Size: 219 1171 200 1135 681 191 1471
Fragments arranged by size:
1471 1171 1135 681 219 200 191
SfoI GGC'GCC
Cuts at: 2210 2210
Size: 5068
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5068
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5068
SmaI CCC'GGG
Cuts at: 1872 2395 1872
Size: 523 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3172 4073 4335 820
Size: 197 926 1229 901 262 1553
Fragments arranged by size:
1553 1229 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2859 493
Size: 2366 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5068
SphI G_CATG'C
Cuts at: 1132 2426 1132
Size: 1294 3774
SspI AAT'ATT
Cuts at: 5 52 2992 5
Size: 47 2940 2081
Fragments arranged by size:
2940 2081 47
StuI AGG'CCT
Cuts at: 1517 2237 1517
Size: 720 4348
StyI C'CwwG_G
Cuts at: 513 1065 2507 513
Size: 552 1442 3074
Fragments arranged by size:
3074 1442 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3095 3112 3265 3450 3895 325
Size: 1255 1515 17 153 185 445 1498
Fragments arranged by size:
1515 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3314 5026 96
Size: 274 80 33 51 1443 1337 1712 138
Fragments arranged by size:
1712 1443 1337 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5028 5060 5028
Size: 32 5036
Tth111I GACn'n_nGTC
Cuts at: 1316 2174 1316
Size: 858 4210
XbaI T'CTAG_A
Cuts at: 2404 2404
Size: 5068
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5068
XmaI C'CCGG_G
Cuts at: 1870 2393 1870
Size: 523 4545
XmnI GAAnn'nnTTC
Cuts at: 3197 3197
Size: 5068
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2876 276
Size: 53 83 186 936 1342 2468
Fragments arranged by size:
2468 1342 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI XcmI