Restriction map for
pFN21AE0119
(Circular) (Six-base) MAPSORT of: pFN21AE0119.seq Check: 2230 from: 1 to: 5728
pFN21AE0119 5728 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3538 278
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2149 2605 3071 1937
Size: 212 456 466 4594
Fragments arranged by size:
4594 466 456 212
Acc65I G'GTAC_C
Cuts at: 3049 3049
Size: 5728
AclI AA'CG_TT
Cuts at: 1563 3855 4228 1563
Size: 2292 373 3063
Fragments arranged by size:
3063 2292 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2301 3791 5175 5443 1632
Size: 669 1490 1384 268 1917
Fragments arranged by size:
1917 1490 1384 669 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5531
AflIII A'CryG_T
Cuts at: 1203 2479 4627 5604 1203
Size: 1276 2148 977 1327
Fragments arranged by size:
2148 1327 1276 977
AgeI A'CCGG_T
Cuts at: 4559 4559
Size: 5728
AhdI GACnn_n'nnGTC
Cuts at: 4457 4457
Size: 5728
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5728
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5728
AlwNI CAG_nnn'CTG
Cuts at: 3471 5043 3471
Size: 1572 4156
ApaLI G'TGCA_C
Cuts at: 2436 3785 4941 2436
Size: 1349 1156 3223
Fragments arranged by size:
3223 1349 1156
ApoI r'AATT_y
Cuts at: 1493 2164 2172 2324 2338 2509 2619 2648 2853
Size: 671 8 152 14 171 110 29 205
Cuts at: 2853 3037 3263 3415 5524 1493
Size: 184 226 152 2109 1697
Fragments arranged by size:
2109 1697 671 226 205 184 171 152
152 110 29 14 8
AseI AT'TA_AT
Cuts at: 160 1032 4282 160
Size: 872 3250 1606
Fragments arranged by size:
3250 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
AvaI C'yCGr_G
Cuts at: 1870 1943 3053 1870
Size: 73 1110 4545
Fragments arranged by size:
4545 1110 73
BamHI G'GATC_C
Cuts at: 3058 3425 3058
Size: 367 5361
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3049 4504 618
Size: 325 217 627 1262 1455 1842
Fragments arranged by size:
1842 1455 1262 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3047 1391
Size: 1656 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2124 928
Size: 971 225 4532
Fragments arranged by size:
4532 971 225
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2918 2952 3919 3953 1903
Size: 34 981 34 967 34 3678
Fragments arranged by size:
3678 981 967 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3620 4830 3620
Size: 1210 4518
BclI T'GATC_A
Cuts at: 1548 2521 1548
Size: 973 4755
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4339 136
Size: 107 122 71 631 605 2667 1525
Fragments arranged by size:
2667 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5723 5723
Size: 5728
BlpI GC'TnA_GC
Cuts at: 3145 3145
Size: 5728
Bme1580I G_kGCm'C
Cuts at: 1790 2440 3789 4945 1790
Size: 650 1349 1156 2573
Fragments arranged by size:
2573 1349 1156 650
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5728
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2972 3459 4417
Size: 416 53 83 399 24 1555 487 958
Cuts at: 4417 442
Size: 1753
Fragments arranged by size:
1753 1555 958 487 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5728
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2168 2168
Size: 5728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2103 4388 1239
Size: 864 2285 2579
Fragments arranged by size:
2579 2285 864
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3853 4718 5016 3853
Size: 865 298 4565
Fragments arranged by size:
4565 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4391 882
Size: 617 2892 2219
Fragments arranged by size:
2892 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3519 493
Size: 711 2315 2702
Fragments arranged by size:
2702 2315 711
BsaBI GATnn'nnATC
Cuts at: 5722 5722
Size: 5728
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3535 3917 4552 275
Size: 53 83 186 936 2002 382 635 1451
Fragments arranged by size:
2002 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4161 4559 4833 4980 5391 4161
Size: 398 274 147 411 4498
Fragments arranged by size:
4498 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5503
Fragments arranged by size:
5503 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5728
BseYI C'CCAG_C
Cuts at: 2823 4931 5427 2823
Size: 2108 496 3124
Fragments arranged by size:
3124 2108 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5155
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3178 3939 4967 1540
Size: 275 186 1177 761 1028 2301
Fragments arranged by size:
2301 1177 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2440 3789 3874 4945 2440
Size: 1349 85 1071 3223
Fragments arranged by size:
3223 1349 1071 85
BsmI GAATG_Cn'
Cuts at: 3239 3332 3239
Size: 93 5635
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2440 3047 3789 3874 4945 1391
Size: 399 650 607 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 650 607 399 85
BspEI T'CCGG_A
Cuts at: 5391 5391
Size: 5728
BspHI T'CATG_A
Cuts at: 3617 5347 3617
Size: 1730 3998
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3083 844
Size: 2239 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2830 3615 1394
Size: 429 1007 785 3507
Fragments arranged by size:
3507 1007 785 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4223 4397 65
Size: 1183 2975 174 1396
Fragments arranged by size:
2975 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4372 4559 1700
Size: 110 2562 187 2869
Fragments arranged by size:
2869 2562 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5728
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5728
BssSI C'ACGA_G
Cuts at: 3788 4800 3788
Size: 1012 4716
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5728
BstBI TT'CG_AA
Cuts at: 4532 4532
Size: 5728
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5728
BstYI r'GATC_y
Cuts at: 1972 2716 3058 3425 3810 3827 5279 5365 5377
Size: 744 342 367 385 17 1452 86 12
Cuts at: 5377 5723 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 744 385 367 346 342 86
17 12
BstZ17I GTA'TAC
Cuts at: 2606 2606
Size: 5728
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4989
Fragments arranged by size:
4989 683 56
BtsI GCAGTG_nn'
Cuts at: 3244 4038 4058 3244
Size: 794 20 4914
Fragments arranged by size:
4914 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5728
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5693
DraI TTT'AAA
Cuts at: 2425 2710 3032 2425
Size: 285 322 5121
Fragments arranged by size:
5121 322 285
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4735 809
Size: 507 3419 1802
Fragments arranged by size:
3419 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3175 4064 5693
Size: 54 1425 50 275 18 1345 889 1629
Cuts at: 5693 8
Size: 43
Fragments arranged by size:
1629 1425 1345 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3175 1537
Size: 275 1363 4090
Fragments arranged by size:
4090 1363 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3658 1353
Size: 2305 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4315 4689 4835 387
Size: 1064 138 2726 374 146 1280
Fragments arranged by size:
2726 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2103 2301 3791 4388 5175 5443 1239
Size: 393 471 198 1490 597 787 268 1524
Fragments arranged by size:
1524 1490 787 597 471 393 268 198
EcoRI G'AATT_C
Cuts at: 2172 3037 3415 2172
Size: 865 378 4485
Fragments arranged by size:
4485 865 378
FspI TGC'GCA
Cuts at: 4234 4234
Size: 5728
HaeII r_GCGC'y
Cuts at: 1678 4875 1678
Size: 3197 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4369 4401 4443 4475 1373
Size: 32 135 32 2797 32 42 32 2626
Fragments arranged by size:
2797 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3072 3318 669
Size: 988 281 1134 246 3079
Fragments arranged by size:
3079 1134 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5728
HpaI GTT'AAC
Cuts at: 3318 3318
Size: 5728
KpnI G_GTAC'C
Cuts at: 3053 3053
Size: 5728
MfeI C'AATT_G
Cuts at: 2224 3327 2224
Size: 1103 4625
MluI A'CGCG_T
Cuts at: 5604 5604
Size: 5728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4842 5026 1305
Size: 3537 184 2007
Fragments arranged by size:
3537 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3906
Fragments arranged by size:
3906 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2484 2892 3686 4045 4204 518
Size: 648 843 475 408 794 359 159 2042
Fragments arranged by size:
2042 843 794 648 475 408 359 159
MspA1I CmG'CkG
Cuts at: 1606 3144 3821 4969 5214 1606
Size: 1538 677 1148 245 2120
Fragments arranged by size:
2120 1538 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5176
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5728
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5728
NotI GC'GGCC_GC
Cuts at: 3175 3175
Size: 5728
NspI r_CATG'y
Cuts at: 1132 1229 2483 2576 3086 4631 1132
Size: 97 1254 93 510 1545 2229
Fragments arranged by size:
2229 1545 1254 510 97 93
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5728
PciI A'CATG_T
Cuts at: 2479 4627 2479
Size: 2148 3580
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5728
PfoI T'CCnGG_A
Cuts at: 2347 2347
Size: 5728
PmeI GTTT'AAAC
Cuts at: 3032 3032
Size: 5728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3797 3829 5310 5342 3797
Size: 32 1481 32 4183
Fragments arranged by size:
4183 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5728
PsiI TTA'TAA
Cuts at: 3298 3298
Size: 5728
PstI C_TGCA'G
Cuts at: 830 3080 830
Size: 2250 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5728
SalI G'TCGA_C
Cuts at: 1936 3070 1936
Size: 1134 4594
SbfI CC_TGCA'GG
Cuts at: 3080 3080
Size: 5728
ScaI AGT'ACT
Cuts at: 2314 3976 5688 2314
Size: 1662 1712 2354
Fragments arranged by size:
2354 1712 1662
SfcI C'TryA_G
Cuts at: 826 1045 3002 3076 4211 4892 5083 826
Size: 219 1957 74 1135 681 191 1471
Fragments arranged by size:
1957 1471 1135 681 219 191 74
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5728
SmaI CCC'GGG
Cuts at: 1872 3055 1872
Size: 1183 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3832 4733 4995 820
Size: 197 926 1889 901 262 1553
Fragments arranged by size:
1889 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3519 493
Size: 3026 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5728
SphI G_CATG'C
Cuts at: 1132 2576 3086 1132
Size: 1444 510 3774
Fragments arranged by size:
3774 1444 510
SspI AAT'ATT
Cuts at: 5 52 2239 3652 5
Size: 47 2187 1413 2081
Fragments arranged by size:
2187 2081 1413 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5728
StyI C'CwwG_G
Cuts at: 513 1065 3167 513
Size: 552 2102 3074
Fragments arranged by size:
3074 2102 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3755 3772 3925 4110 4555 325
Size: 1255 2175 17 153 185 445 1498
Fragments arranged by size:
2175 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2312 2383 3974
Size: 274 80 33 51 1443 335 71 1591
Cuts at: 3974 5686 96
Size: 1712 138
Fragments arranged by size:
1712 1591 1443 335 274 138 80 71
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5688 5720 5688
Size: 32 5696
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5728
XbaI T'CTAG_A
Cuts at: 3064 3064
Size: 5728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5728
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5728
XmaI C'CCGG_G
Cuts at: 1870 3053 1870
Size: 1183 4545
XmnI GAAnn'nnTTC
Cuts at: 2853 3857 2853
Size: 1004 4724
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3536 276
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI