Restriction map for
pFN21AE0114
(Circular) (Six-base) MAPSORT of: pFN21AE0114.seq Check: 9227 from: 1 to: 5629
pFN21AE0114 5629 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2166 3439 278
Size: 53 83 186 936 630 1273 2468
Fragments arranged by size:
2468 1273 936 630 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2052 2972 1937
Size: 115 920 4594
Fragments arranged by size:
4594 920 115
Acc65I G'GTAC_C
Cuts at: 2950 2950
Size: 5629
AclI AA'CG_TT
Cuts at: 1563 3756 4129 1563
Size: 2193 373 3063
Fragments arranged by size:
3063 2193 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2495 2531 2855 3692 5076 5344 1632
Size: 863 36 324 837 1384 268 1917
Fragments arranged by size:
1917 1384 863 837 324 268 36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5432
AflIII A'CryG_T
Cuts at: 1203 4528 5505 1203
Size: 3325 977 1327
Fragments arranged by size:
3325 1327 977
AgeI A'CCGG_T
Cuts at: 4460 4460
Size: 5629
AhdI GACnn_n'nnGTC
Cuts at: 4358 4358
Size: 5629
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5629
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5629
AlwNI CAG_nnn'CTG
Cuts at: 2470 2527 2791 3372 4944 2470
Size: 57 264 581 1572 3155
Fragments arranged by size:
3155 1572 581 264 57
ApaLI G'TGCA_C
Cuts at: 2446 3686 4842 2446
Size: 1240 1156 3233
Fragments arranged by size:
3233 1240 1156
ApoI r'AATT_y
Cuts at: 1493 2938 3164 3316 5425 1493
Size: 1445 226 152 2109 1697
Fragments arranged by size:
2109 1697 1445 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4183 160
Size: 872 3151 1606
Fragments arranged by size:
3151 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
AvaI C'yCGr_G
Cuts at: 1870 1943 2954 1870
Size: 73 1011 4545
Fragments arranged by size:
4545 1011 73
BamHI G'GATC_C
Cuts at: 2959 3326 2959
Size: 367 5262
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2950 4405 618
Size: 325 217 627 221 942 1455 1842
Fragments arranged by size:
1842 1455 942 627 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2247 2948 1391
Size: 856 701 4072
Fragments arranged by size:
4072 856 701
BbeI G_GCGC'C
Cuts at: 2012 2012
Size: 5629
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2158 928
Size: 971 259 4399
Fragments arranged by size:
4399 971 259
BbvCI CC'TCA_GC
Cuts at: 2709 2709
Size: 5629
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3820 3854 1903
Size: 34 1883 34 3678
Fragments arranged by size:
3678 1883 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3521 4731 3521
Size: 1210 4419
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5629
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4240 136
Size: 107 122 71 631 605 2568 1525
Fragments arranged by size:
2568 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5624 5624
Size: 5629
BlpI GC'TnA_GC
Cuts at: 3046 3046
Size: 5629
Bme1580I G_kGCm'C
Cuts at: 1790 2450 3690 4846 1790
Size: 660 1240 1156 2573
Fragments arranged by size:
2573 1240 1156 660
BmgBI CAC'GTC
Cuts at: 1318 2152 2344 1318
Size: 834 192 4603
Fragments arranged by size:
4603 834 192
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3360 4318 442
Size: 416 53 83 399 24 1943 958 1753
Fragments arranged by size:
1943 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2231 2462 4289 1239
Size: 992 231 1827 2579
Fragments arranged by size:
2579 1827 992 231
Bpu10I CC'TnA_GC
Cuts at: 2241 2709 2241
Size: 468 5161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3754 4619 4917 3754
Size: 865 298 4466
Fragments arranged by size:
4466 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4292 882
Size: 617 2793 2219
Fragments arranged by size:
2793 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3420 493
Size: 711 2216 2702
Fragments arranged by size:
2702 2216 711
BsaBI GATnn'nnATC
Cuts at: 5623 5623
Size: 5629
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2163 3436 3818
Size: 53 83 186 936 476 154 1273 382
Cuts at: 3818 4453 275
Size: 635 1451
Fragments arranged by size:
1451 1273 936 635 476 382 186 154
83 53
BsaWI w'CCGG_w
Cuts at: 4062 4460 4734 4881 5292 4062
Size: 398 274 147 411 4399
Fragments arranged by size:
4399 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5404
Fragments arranged by size:
5404 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2221 1186
Size: 1035 4594
BseYI C'CCAG_C
Cuts at: 2550 2697 2784 2829 2921 4832 5328 2550
Size: 147 87 45 92 1911 496 2851
Fragments arranged by size:
2851 1911 496 147 92 87 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2408 2483 1163
Size: 573 672 75 4309
Fragments arranged by size:
4309 672 573 75
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3079 3840 4868 1540
Size: 275 186 1078 761 1028 2301
Fragments arranged by size:
2301 1078 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2247 2450 2642 3690 3775 4846 2247
Size: 203 192 1048 85 1071 3030
Fragments arranged by size:
3030 1071 1048 203 192 85
BsmI GAATG_Cn'
Cuts at: 2523 3140 3233 2523
Size: 617 93 4919
Fragments arranged by size:
4919 617 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2142 2750 2142
Size: 608 5021
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2247 2450 2642 2948 3690 3775 4846
Size: 399 457 203 192 306 742 85 1071
Cuts at: 4846 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 457 399 306 203 192
85
BspEI T'CCGG_A
Cuts at: 5292 5292
Size: 5629
BspHI T'CATG_A
Cuts at: 3518 5248 3518
Size: 1730 3899
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2984 844
Size: 2140 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3516 1394
Size: 429 1693 3507
Fragments arranged by size:
3507 1693 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4124 4298 65
Size: 1183 2876 174 1396
Fragments arranged by size:
2876 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4273 4460 1700
Size: 110 2463 187 2869
Fragments arranged by size:
2869 2463 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5629
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5629
BssSI C'ACGA_G
Cuts at: 2282 3689 4701 2282
Size: 1407 1012 3210
Fragments arranged by size:
3210 1407 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2470 794
Size: 1676 3953
BstBI TT'CG_AA
Cuts at: 4433 4433
Size: 5629
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5629
BstYI r'GATC_y
Cuts at: 1972 2466 2959 3326 3711 3728 5180 5266 5278
Size: 494 493 367 385 17 1452 86 12
Cuts at: 5278 5624 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 494 493 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2805 2805
Size: 5629
BtgI C'CryG_G
Cuts at: 513 1065 2004 2580 513
Size: 552 939 576 3562
Fragments arranged by size:
3562 939 576 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4890
Fragments arranged by size:
4890 683 56
BtsI GCAGTG_nn'
Cuts at: 2108 2559 3145 3939 3959 2108
Size: 451 586 794 20 3778
Fragments arranged by size:
3778 794 586 451 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5629
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2725 2760 529
Size: 35 2161 35 3398
Fragments arranged by size:
3398 2161 35 35
DraI TTT'AAA
Cuts at: 2933 2933
Size: 5629
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4636 809
Size: 507 3320 1802
Fragments arranged by size:
3320 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2307 2370 2577
Size: 54 1425 50 275 18 477 63 207
Cuts at: 2577 3076 3965 5594 8
Size: 499 889 1629 43
Fragments arranged by size:
1629 1425 889 499 477 275 207 63
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3076 1537
Size: 275 1264 4090
Fragments arranged by size:
4090 1264 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2507 3559 1353
Size: 1154 1052 3423
Fragments arranged by size:
3423 1154 1052
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4216 4590 4736 387
Size: 1064 138 2627 374 146 1280
Fragments arranged by size:
2627 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2245 2245
Size: 5629
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 2462 2495 2531 2855 3692 4289
Size: 393 599 231 33 36 324 837 597
Cuts at: 4289 5076 5344 1239
Size: 787 268 1524
Fragments arranged by size:
1524 837 787 599 597 393 324 268
231 36 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2531 2720 2531
Size: 189 5440
EcoO109I rG'GnC_Cy
Cuts at: 2524 2713 2850 2524
Size: 189 137 5303
Fragments arranged by size:
5303 189 137
EcoRI G'AATT_C
Cuts at: 2938 3316 2938
Size: 378 5251
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 5629
FspI TGC'GCA
Cuts at: 2258 4135 2258
Size: 1877 3752
FspAI rTGC'GCAy
Cuts at: 2258 2258
Size: 5629
HaeII r_GCGC'y
Cuts at: 1678 2012 2732 2928 4776 1678
Size: 334 720 196 1848 2531
Fragments arranged by size:
2531 1848 720 334 196
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2624 2656 4270 4302 4344
Size: 32 135 32 1052 32 1614 32 42
Cuts at: 4344 4376 1373
Size: 32 2626
Fragments arranged by size:
2626 1614 1052 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2767 2973 3219 669
Size: 988 281 829 206 246 3079
Fragments arranged by size:
3079 988 829 281 246 206
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5629
HpaI GTT'AAC
Cuts at: 3219 3219
Size: 5629
KasI G'GCGC_C
Cuts at: 2008 2008
Size: 5629
KpnI G_GTAC'C
Cuts at: 2954 2954
Size: 5629
MfeI C'AATT_G
Cuts at: 3228 3228
Size: 5629
MluI A'CGCG_T
Cuts at: 5505 5505
Size: 5629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2270 4743 4927 1305
Size: 965 2473 184 2007
Fragments arranged by size:
2473 2007 965 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2579 10
Size: 54 1425 343 747 3060
Fragments arranged by size:
3060 1425 747 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2453 2459 3587 3946 4105 518
Size: 648 1287 6 1128 359 159 2042
Fragments arranged by size:
2042 1287 1128 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2122 2697 2708 2833 3045 3722 4870 5115
Size: 516 575 11 125 212 677 1148 245
Cuts at: 5115 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 575 516 245 212 125
11
NarI GG'CG_CC
Cuts at: 2009 2009
Size: 5629
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4138
Fragments arranged by size:
4138 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5629
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5629
NotI GC'GGCC_GC
Cuts at: 3076 3076
Size: 5629
NspI r_CATG'y
Cuts at: 1132 1229 2263 2987 4532 1132
Size: 97 1034 724 1545 2229
Fragments arranged by size:
2229 1545 1034 724 97
PasI CC'CwG_GG
Cuts at: 1348 2689 1348
Size: 1341 4288
PciI A'CATG_T
Cuts at: 4528 4528
Size: 5629
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2565 2626 2791 1108
Size: 1457 61 165 3946
Fragments arranged by size:
3946 1457 165 61
PfoI T'CCnGG_A
Cuts at: 2211 2619 2211
Size: 408 5221
PmeI GTTT'AAAC
Cuts at: 2933 2933
Size: 5629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 5211 5243 3698
Size: 32 1481 32 4084
Fragments arranged by size:
4084 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2524 2850 2524
Size: 326 5303
PshAI GACnn'nnGTC
Cuts at: 1613 2762 1613
Size: 1149 4480
PsiI TTA'TAA
Cuts at: 3199 3199
Size: 5629
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2066 2098 2066
Size: 32 5597
PstI C_TGCA'G
Cuts at: 830 2601 2724 2981 830
Size: 1771 123 257 3478
Fragments arranged by size:
3478 1771 257 123
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5629
PvuII CAG'CTG
Cuts at: 2833 2833
Size: 5629
SacI G_AGCT'C
Cuts at: 2247 2247
Size: 5629
SalI G'TCGA_C
Cuts at: 1936 2971 1936
Size: 1035 4594
SbfI CC_TGCA'GG
Cuts at: 2981 2981
Size: 5629
ScaI AGT'ACT
Cuts at: 3877 5589 3877
Size: 1712 3917
SfcI C'TryA_G
Cuts at: 826 1045 2597 2720 2909 2977 4112 4793 4984
Size: 219 1552 123 189 68 1135 681 191
Cuts at: 4984 826
Size: 1471
Fragments arranged by size:
1552 1471 1135 681 219 191 189 123
68
SfoI GGC'GCC
Cuts at: 2010 2010
Size: 5629
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5629
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5629
SmaI CCC'GGG
Cuts at: 1872 2956 1872
Size: 1084 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3733 4634 4896 820
Size: 197 926 1790 901 262 1553
Fragments arranged by size:
1790 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3420 493
Size: 2927 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5629
SphI G_CATG'C
Cuts at: 1132 2987 1132
Size: 1855 3774
SspI AAT'ATT
Cuts at: 5 52 3553 5
Size: 47 3501 2081
Fragments arranged by size:
3501 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5629
StyI C'CwwG_G
Cuts at: 513 1065 2004 2880 3068 513
Size: 552 939 876 188 3074
Fragments arranged by size:
3074 939 876 552 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2334 2564 3656 3673 3826 4011 4456
Size: 1255 754 230 1092 17 153 185 445
Cuts at: 4456 325
Size: 1498
Fragments arranged by size:
1498 1255 1092 754 445 230 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3875 5587 96
Size: 274 80 33 51 1443 1898 1712 138
Fragments arranged by size:
1898 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2188 2220 5589 5621 2188
Size: 32 3369 32 2196
Fragments arranged by size:
3369 2196 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5629
XbaI T'CTAG_A
Cuts at: 2965 2965
Size: 5629
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5629
XmaI C'CCGG_G
Cuts at: 1870 2954 1870
Size: 1084 4545
XmnI GAAnn'nnTTC
Cuts at: 2078 3758 2078
Size: 1680 3949
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2164 3437 276
Size: 53 83 186 936 630 1273 2468
Fragments arranged by size:
2468 1273 936 630 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BstXI BstZ17I DraIII EcoRV FseI NaeI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI