Restriction map for
pFN21AE0113
(Circular) (Six-base) MAPSORT of: pFN21AE0113.seq Check: 764 from: 1 to: 4969
pFN21AE0113 4969 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2779 278
Size: 53 83 186 936 1243 2468
Fragments arranged by size:
2468 1243 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2312 1937
Size: 375 4594
Acc65I G'GTAC_C
Cuts at: 2290 2290
Size: 4969
AclI AA'CG_TT
Cuts at: 1563 3096 3469 1563
Size: 1533 373 3063
Fragments arranged by size:
3063 1533 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3032 4416 4684 1632
Size: 1400 1384 268 1917
Fragments arranged by size:
1917 1400 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4772
AflIII A'CryG_T
Cuts at: 1203 3868 4845 1203
Size: 2665 977 1327
Fragments arranged by size:
2665 1327 977
AgeI A'CCGG_T
Cuts at: 3800 3800
Size: 4969
AhdI GACnn_n'nnGTC
Cuts at: 3698 3698
Size: 4969
AleI CACnn'nnGTG
Cuts at: 1166 2036 1166
Size: 870 4099
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4969
AlwNI CAG_nnn'CTG
Cuts at: 2712 4284 2712
Size: 1572 3397
ApaLI G'TGCA_C
Cuts at: 3026 4182 3026
Size: 1156 3813
ApoI r'AATT_y
Cuts at: 1493 2278 2504 2656 4765 1493
Size: 785 226 152 2109 1697
Fragments arranged by size:
2109 1697 785 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3523 160
Size: 872 2491 1606
Fragments arranged by size:
2491 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4969
AvaI C'yCGr_G
Cuts at: 1870 1943 2294 1870
Size: 73 351 4545
Fragments arranged by size:
4545 351 73
BamHI G'GATC_C
Cuts at: 2299 2666 2299
Size: 367 4602
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2290 3745 618
Size: 325 217 627 503 1455 1842
Fragments arranged by size:
1842 1455 627 503 325 217
BanII G_rGCy'C
Cuts at: 1391 2288 1391
Size: 897 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3998
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3160 3194 1903
Size: 34 1223 34 3678
Fragments arranged by size:
3678 1223 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2861 4071 2861
Size: 1210 3759
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4969
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2072 2081 3580
Size: 107 122 71 631 605 400 9 1499
Cuts at: 3580 136
Size: 1525
Fragments arranged by size:
1525 1499 631 605 400 122 107 71
9
BglII A'GATC_T
Cuts at: 4964 4964
Size: 4969
BlpI GC'TnA_GC
Cuts at: 2386 2386
Size: 4969
Bme1580I G_kGCm'C
Cuts at: 1790 3030 4186 1790
Size: 1240 1156 2573
Fragments arranged by size:
2573 1240 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4969
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2700 3658 442
Size: 416 53 83 399 24 1283 958 1753
Fragments arranged by size:
1753 1283 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4969
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3629 1239
Size: 2390 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3094 3959 4257 3094
Size: 865 298 3806
Fragments arranged by size:
3806 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2162 3632 882
Size: 617 663 1470 2219
Fragments arranged by size:
2219 1470 663 617
BsaAI yAC'GTr
Cuts at: 493 1204 2760 493
Size: 711 1556 2702
Fragments arranged by size:
2702 1556 711
BsaBI GATnn'nnATC
Cuts at: 4963 4963
Size: 4969
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2776 3158 3793 275
Size: 53 83 186 936 1243 382 635 1451
Fragments arranged by size:
1451 1243 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3402 3800 4074 4221 4632 3402
Size: 398 274 147 411 3739
Fragments arranged by size:
3739 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4744
Fragments arranged by size:
4744 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2159 1186
Size: 973 3996
BseYI C'CCAG_C
Cuts at: 4172 4668 4172
Size: 496 4473
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2213 1163
Size: 573 477 3919
Fragments arranged by size:
3919 573 477
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2419 3180 4208 1540
Size: 275 186 418 761 1028 2301
Fragments arranged by size:
2301 1028 761 418 275 186
BsiHKAI G_wGCw'C
Cuts at: 3030 3115 4186 3030
Size: 85 1071 3813
Fragments arranged by size:
3813 1071 85
BsmI GAATG_Cn'
Cuts at: 2480 2573 2480
Size: 93 4876
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2288 3030 3115 4186 1391
Size: 399 498 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 498 399 85
BspEI T'CCGG_A
Cuts at: 4632 4632
Size: 4969
BspHI T'CATG_A
Cuts at: 2858 4588 2858
Size: 1730 3239
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2118 2324 844
Size: 1274 206 3489
Fragments arranged by size:
3489 1274 206
BsrBI CCG'CTC
Cuts at: 1394 1823 2856 1394
Size: 429 1033 3507
Fragments arranged by size:
3507 1033 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2061 3464 3638 65
Size: 1183 813 1403 174 1396
Fragments arranged by size:
1403 1396 1183 813 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3613 3800 1700
Size: 110 1803 187 2869
Fragments arranged by size:
2869 1803 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4969
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4969
BssSI C'ACGA_G
Cuts at: 3029 4041 3029
Size: 1012 3957
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4969
BstBI TT'CG_AA
Cuts at: 3773 3773
Size: 4969
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4969
BstXI CCAn_nnnn'nTGG
Cuts at: 2038 2038
Size: 4969
BstYI r'GATC_y
Cuts at: 1972 2299 2666 3051 3068 4520 4606 4618 4964
Size: 327 367 385 17 1452 86 12 346
Cuts at: 4964 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 327 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2031 2263 513
Size: 552 966 232 3219
Fragments arranged by size:
3219 966 552 232
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2053 487
Size: 683 56 827 3403
Fragments arranged by size:
3403 827 683 56
BtsI GCAGTG_nn'
Cuts at: 2485 3279 3299 2485
Size: 794 20 4155
Fragments arranged by size:
4155 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4969
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4934
DraI TTT'AAA
Cuts at: 2273 2273
Size: 4969
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3976 809
Size: 507 2660 1802
Fragments arranged by size:
2660 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2202 2416 3305
Size: 54 1425 50 275 18 372 214 889
Cuts at: 3305 4934 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 372 275 214 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2416 1537
Size: 275 604 4090
Fragments arranged by size:
4090 604 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2899 1353
Size: 1546 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3556 3930 4076 387
Size: 1064 138 1967 374 146 1280
Fragments arranged by size:
1967 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3032 3629 4416 4684 1239
Size: 393 1400 597 787 268 1524
Fragments arranged by size:
1524 1400 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2082 2082
Size: 4969
EcoRI G'AATT_C
Cuts at: 2278 2656 2278
Size: 378 4591
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 4969
FspI TGC'GCA
Cuts at: 3475 3475
Size: 4969
HaeII r_GCGC'y
Cuts at: 1678 4116 1678
Size: 2438 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3610 3642 3684 3716 1373
Size: 32 135 32 2038 32 42 32 2626
Fragments arranged by size:
2626 2038 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2313 2559 669
Size: 988 281 375 246 3079
Fragments arranged by size:
3079 988 375 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4969
HpaI GTT'AAC
Cuts at: 2559 2559
Size: 4969
KpnI G_GTAC'C
Cuts at: 2294 2294
Size: 4969
MfeI C'AATT_G
Cuts at: 2568 2568
Size: 4969
MluI A'CGCG_T
Cuts at: 4845 4845
Size: 4969
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4083 4267 1305
Size: 2778 184 2007
Fragments arranged by size:
2778 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3147
Fragments arranged by size:
3147 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2036 2927 3286 3445 518
Size: 648 870 891 359 159 2042
Fragments arranged by size:
2042 891 870 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2385 3062 4210 4455 1606
Size: 779 677 1148 245 2120
Fragments arranged by size:
2120 1148 779 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4417
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4969
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4969
NotI GC'GGCC_GC
Cuts at: 2416 2416
Size: 4969
NspI r_CATG'y
Cuts at: 1132 1229 2327 3872 1132
Size: 97 1098 1545 2229
Fragments arranged by size:
2229 1545 1098 97
PasI CC'CwG_GG
Cuts at: 1348 2077 1348
Size: 729 4240
PciI A'CATG_T
Cuts at: 3868 3868
Size: 4969
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4969
PmeI GTTT'AAAC
Cuts at: 2273 2273
Size: 4969
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3038 3070 4551 4583 3038
Size: 32 1481 32 3424
Fragments arranged by size:
3424 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4969
PsiI TTA'TAA
Cuts at: 2539 2539
Size: 4969
PstI C_TGCA'G
Cuts at: 830 2121 2321 830
Size: 1291 200 3478
Fragments arranged by size:
3478 1291 200
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4969
SalI G'TCGA_C
Cuts at: 1936 2311 1936
Size: 375 4594
SbfI CC_TGCA'GG
Cuts at: 2321 2321
Size: 4969
ScaI AGT'ACT
Cuts at: 3217 4929 3217
Size: 1712 3257
SfcI C'TryA_G
Cuts at: 826 1045 2117 2317 3452 4133 4324 826
Size: 219 1072 200 1135 681 191 1471
Fragments arranged by size:
1471 1135 1072 681 219 200 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2081 2081
Size: 4969
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4969
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4969
SmaI CCC'GGG
Cuts at: 1872 2296 1872
Size: 424 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3073 3974 4236 820
Size: 197 926 1130 901 262 1553
Fragments arranged by size:
1553 1130 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2760 493
Size: 2267 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4969
SphI G_CATG'C
Cuts at: 1132 2327 1132
Size: 1195 3774
SspI AAT'ATT
Cuts at: 5 52 2893 5
Size: 47 2841 2081
Fragments arranged by size:
2841 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4969
StyI C'CwwG_G
Cuts at: 513 1065 2172 2205 2408 513
Size: 552 1107 33 203 3074
Fragments arranged by size:
3074 1107 552 203 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2996 3013 3166 3351 3796 325
Size: 1255 1416 17 153 185 445 1498
Fragments arranged by size:
1498 1416 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3215 4927 96
Size: 274 80 33 51 1443 1238 1712 138
Fragments arranged by size:
1712 1443 1238 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2202 2234 4929 4961 2202
Size: 32 2695 32 2210
Fragments arranged by size:
2695 2210 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4969
XbaI T'CTAG_A
Cuts at: 2305 2305
Size: 4969
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2035 2074 2011
Size: 24 39 4906
Fragments arranged by size:
4906 39 24
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4969
XmaI C'CCGG_G
Cuts at: 1870 2294 1870
Size: 424 4545
XmnI GAAnn'nnTTC
Cuts at: 3098 3098
Size: 4969
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2777 276
Size: 53 83 186 936 1243 2468
Fragments arranged by size:
2468 1243 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfoI SrfI SwaI