Restriction map for
pFN21AE0110
(Circular) (Six-base) MAPSORT of: pFN21AE0110.seq Check: 2787 from: 1 to: 5482
pFN21AE0110 5482 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3292 278
Size: 53 83 186 936 1756 2468
Fragments arranged by size:
2468 1756 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2825 1937
Size: 888 4594
Acc65I G'GTAC_C
Cuts at: 2803 2803
Size: 5482
AclI AA'CG_TT
Cuts at: 1563 3609 3982 1563
Size: 2046 373 3063
Fragments arranged by size:
3063 2046 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2229 3545 4929 5197 1632
Size: 597 1316 1384 268 1917
Fragments arranged by size:
1917 1384 1316 597 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5285
AflIII A'CryG_T
Cuts at: 1203 4381 5358 1203
Size: 3178 977 1327
Fragments arranged by size:
3178 1327 977
AgeI A'CCGG_T
Cuts at: 4313 4313
Size: 5482
AhdI GACnn_n'nnGTC
Cuts at: 4211 4211
Size: 5482
AleI CACnn'nnGTG
Cuts at: 1166 2531 1166
Size: 1365 4117
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5482
AlwNI CAG_nnn'CTG
Cuts at: 3225 4797 3225
Size: 1572 3910
ApaLI G'TGCA_C
Cuts at: 3539 4695 3539
Size: 1156 4326
ApoI r'AATT_y
Cuts at: 1493 2195 2379 2791 3017 3169 5278 1493
Size: 702 184 412 226 152 2109 1697
Fragments arranged by size:
2109 1697 702 412 226 184 152
AseI AT'TA_AT
Cuts at: 160 1032 2712 4036 160
Size: 872 1680 1324 1606
Fragments arranged by size:
1680 1606 1324 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
AvaI C'yCGr_G
Cuts at: 1870 1943 2807 1870
Size: 73 864 4545
Fragments arranged by size:
4545 864 73
BamHI G'GATC_C
Cuts at: 2812 3179 2812
Size: 367 5115
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2356 2803 4258 618
Size: 325 217 627 569 447 1455 1842
Fragments arranged by size:
1842 1455 627 569 447 325 217
BanII G_rGCy'C
Cuts at: 1391 2644 2801 1391
Size: 1253 157 4072
Fragments arranged by size:
4072 1253 157
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2555 928
Size: 971 656 3855
Fragments arranged by size:
3855 971 656
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3673 3707 1903
Size: 34 1736 34 3678
Fragments arranged by size:
3678 1736 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3374 4584 3374
Size: 1210 4272
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5482
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4093 136
Size: 107 122 71 631 605 2421 1525
Fragments arranged by size:
2421 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2041 5477 2041
Size: 3436 2046
BlpI GC'TnA_GC
Cuts at: 2467 2899 2467
Size: 432 5050
Bme1580I G_kGCm'C
Cuts at: 1790 2299 2579 3543 4699 1790
Size: 509 280 964 1156 2573
Fragments arranged by size:
2573 1156 964 509 280
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5482
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2688 3213 4171
Size: 416 53 83 399 24 1271 525 958
Cuts at: 4171 442
Size: 1753
Fragments arranged by size:
1753 1271 958 525 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5482
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2763 2763
Size: 5482
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4142 1239
Size: 2903 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2628 3607 4472 4770 2628
Size: 979 865 298 3340
Fragments arranged by size:
3340 979 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4145 882
Size: 617 2646 2219
Fragments arranged by size:
2646 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2111 3273 493
Size: 711 907 1162 2702
Fragments arranged by size:
2702 1162 907 711
BsaBI GATnn'nnATC
Cuts at: 2429 5476 2429
Size: 3047 2435
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3289 3671 4306 275
Size: 53 83 186 936 1756 382 635 1451
Fragments arranged by size:
1756 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2397 3915 4313 4587 4734 5145 2397
Size: 1518 398 274 147 411 2734
Fragments arranged by size:
2734 1518 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5257
Fragments arranged by size:
5257 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2609 1186
Size: 1423 4059
BseYI C'CCAG_C
Cuts at: 2659 4685 5181 2659
Size: 2026 496 2960
Fragments arranged by size:
2960 2026 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4909
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2932 3693 4721 1540
Size: 275 186 931 761 1028 2301
Fragments arranged by size:
2301 1028 931 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2419 3543 3628 4699 2419
Size: 1124 85 1071 3202
Fragments arranged by size:
3202 1124 1071 85
BsmI GAATG_Cn'
Cuts at: 2393 2993 3086 2393
Size: 600 93 4789
Fragments arranged by size:
4789 600 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2299 2419 2579 2644 2801 3543 3628
Size: 399 509 120 160 65 157 742 85
Cuts at: 3628 4699 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 509 399 160 157 120
85 65
BspEI T'CCGG_A
Cuts at: 5145 5145
Size: 5482
BspHI T'CATG_A
Cuts at: 2604 3371 5101 2604
Size: 767 1730 2985
Fragments arranged by size:
2985 1730 767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2837 844
Size: 1993 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2257 3369 1394
Size: 429 434 1112 3507
Fragments arranged by size:
3507 1112 434 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2567 3977 4151 65
Size: 1183 1319 1410 174 1396
Fragments arranged by size:
1410 1396 1319 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4126 4313 1700
Size: 110 2316 187 2869
Fragments arranged by size:
2869 2316 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5482
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5482
BssSI C'ACGA_G
Cuts at: 3542 4554 3542
Size: 1012 4470
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2018 794
Size: 1224 4258
BstBI TT'CG_AA
Cuts at: 4286 4286
Size: 5482
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5482
BstXI CCAn_nnnn'nTGG
Cuts at: 2533 2533
Size: 5482
BstYI r'GATC_y
Cuts at: 1972 2041 2812 3179 3564 3581 5033 5119 5131
Size: 69 771 367 385 17 1452 86 12
Cuts at: 5131 5477 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 771 385 367 346 86 69
17 12
Bsu36I CC'TnA_GG
Cuts at: 2592 2592
Size: 5482
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3991
Fragments arranged by size:
3991 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4743
Fragments arranged by size:
4743 683 56
BtsI GCAGTG_nn'
Cuts at: 2998 3792 3812 2998
Size: 794 20 4668
Fragments arranged by size:
4668 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5482
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5447
DraI TTT'AAA
Cuts at: 2786 2786
Size: 5482
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4489 809
Size: 507 3173 1802
Fragments arranged by size:
3173 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2929 3818 5447
Size: 54 1425 50 275 18 1099 889 1629
Cuts at: 5447 8
Size: 43
Fragments arranged by size:
1629 1425 1099 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2929 1537
Size: 275 1117 4090
Fragments arranged by size:
4090 1117 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3412 1353
Size: 2059 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4069 4443 4589 387
Size: 1064 138 2480 374 146 1280
Fragments arranged by size:
2480 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2229 3545 4142 4929 5197 1239
Size: 393 597 1316 597 787 268 1524
Fragments arranged by size:
1524 1316 787 597 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2477 2477
Size: 5482
EcoRI G'AATT_C
Cuts at: 2791 3169 2791
Size: 378 5104
EcoRV GAT'ATC
Cuts at: 2203 2203
Size: 5482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2572 2572
Size: 5482
FspI TGC'GCA
Cuts at: 3988 3988
Size: 5482
HaeII r_GCGC'y
Cuts at: 1678 4629 1678
Size: 2951 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4123 4155 4197 4229 1373
Size: 32 135 32 2551 32 42 32 2626
Fragments arranged by size:
2626 2551 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2826 3072 669
Size: 988 281 888 246 3079
Fragments arranged by size:
3079 988 888 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5482
HpaI GTT'AAC
Cuts at: 3072 3072
Size: 5482
KpnI G_GTAC'C
Cuts at: 2807 2807
Size: 5482
MfeI C'AATT_G
Cuts at: 3081 3081
Size: 5482
MluI A'CGCG_T
Cuts at: 5358 5358
Size: 5482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4596 4780 1305
Size: 3291 184 2007
Fragments arranged by size:
3291 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3660
Fragments arranged by size:
3660 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2531 3440 3799 3958 518
Size: 648 1365 909 359 159 2042
Fragments arranged by size:
2042 1365 909 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2075 2898 3575 4723 4968 1606
Size: 469 823 677 1148 245 2120
Fragments arranged by size:
2120 1148 823 677 469 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3991
Fragments arranged by size:
3991 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5482
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5482
NotI GC'GGCC_GC
Cuts at: 2929 2929
Size: 5482
NspI r_CATG'y
Cuts at: 1132 1229 2016 2659 2840 4385 1132
Size: 97 787 643 181 1545 2229
Fragments arranged by size:
2229 1545 787 643 181 97
PasI CC'CwG_GG
Cuts at: 1348 2473 1348
Size: 1125 4357
PciI A'CATG_T
Cuts at: 4381 4381
Size: 5482
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5482
PfoI T'CCnGG_A
Cuts at: 2406 2406
Size: 5482
PmeI GTTT'AAAC
Cuts at: 2786 2786
Size: 5482
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2537 2569 3551 3583 5064 5096 2537
Size: 32 982 32 1481 32 2923
Fragments arranged by size:
2923 1481 982 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5482
PsiI TTA'TAA
Cuts at: 3052 3052
Size: 5482
PstI C_TGCA'G
Cuts at: 830 2239 2772 2834 830
Size: 1409 533 62 3478
Fragments arranged by size:
3478 1409 533 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5482
PvuII CAG'CTG
Cuts at: 2075 2075
Size: 5482
SalI G'TCGA_C
Cuts at: 1936 2824 1936
Size: 888 4594
SbfI CC_TGCA'GG
Cuts at: 2834 2834
Size: 5482
ScaI AGT'ACT
Cuts at: 3730 5442 3730
Size: 1712 3770
SfcI C'TryA_G
Cuts at: 826 1045 2235 2768 2830 3965 4646 4837 826
Size: 219 1190 533 62 1135 681 191 1471
Fragments arranged by size:
1471 1190 1135 681 533 219 191 62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5482
SmaI CCC'GGG
Cuts at: 1872 2809 1872
Size: 937 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2643 3586 4487 4749 820
Size: 197 926 700 943 901 262 1553
Fragments arranged by size:
1553 943 926 901 700 262 197
SnaBI TAC'GTA
Cuts at: 493 3273 493
Size: 2780 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5482
SphI G_CATG'C
Cuts at: 1132 2016 2659 2840 1132
Size: 884 643 181 3774
Fragments arranged by size:
3774 884 643 181
SspI AAT'ATT
Cuts at: 5 52 2220 3406 5
Size: 47 2168 1186 2081
Fragments arranged by size:
2168 2081 1186 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5482
StyI C'CwwG_G
Cuts at: 513 1065 2004 2921 513
Size: 552 939 917 3074
Fragments arranged by size:
3074 939 917 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3509 3526 3679 3864 4309 325
Size: 1255 1929 17 153 185 445 1498
Fragments arranged by size:
1929 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3728 5440 96
Size: 274 80 33 51 1443 1751 1712 138
Fragments arranged by size:
1751 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2475 2507 5442 5474 2475
Size: 32 2935 32 2483
Fragments arranged by size:
2935 2483 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5482
XbaI T'CTAG_A
Cuts at: 2818 2818
Size: 5482
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5482
XmaI C'CCGG_G
Cuts at: 1870 2807 1870
Size: 937 4545
XmnI GAAnn'nnTTC
Cuts at: 2367 2552 3611 2367
Size: 185 1059 4238
Fragments arranged by size:
4238 1059 185
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3290 276
Size: 53 83 186 936 1756 2468
Fragments arranged by size:
2468 1756 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI