Restriction map for
pFN21AE0108
(Circular) (Six-base) MAPSORT of: pFN21AE0108.seq Check: 1696 from: 1 to: 5659
pFN21AE0108 5659 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2296 2805 3469 278
Size: 53 83 186 936 760 509 664 2468
Fragments arranged by size:
2468 936 760 664 509 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3002 1937
Size: 1065 4594
Acc65I G'GTAC_C
Cuts at: 2018 2980 2018
Size: 962 4697
AclI AA'CG_TT
Cuts at: 1563 3786 4159 1563
Size: 2223 373 3063
Fragments arranged by size:
3063 2223 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2615 2918 3722 5106 5374 1632
Size: 983 303 804 1384 268 1917
Fragments arranged by size:
1917 1384 983 804 303 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5462
AflIII A'CryG_T
Cuts at: 1203 4558 5535 1203
Size: 3355 977 1327
Fragments arranged by size:
3355 1327 977
AgeI A'CCGG_T
Cuts at: 2010 4490 2010
Size: 2480 3179
AhdI GACnn_n'nnGTC
Cuts at: 4388 4388
Size: 5659
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5659
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2205 2743 1184
Size: 1021 538 4100
Fragments arranged by size:
4100 1021 538
AlwNI CAG_nnn'CTG
Cuts at: 3402 4974 3402
Size: 1572 4087
ApaLI G'TGCA_C
Cuts at: 3716 4872 3716
Size: 1156 4503
ApoI r'AATT_y
Cuts at: 1493 2149 2233 2340 2968 3194 3346 5455 1493
Size: 656 84 107 628 226 152 2109 1697
Fragments arranged by size:
2109 1697 656 628 226 152 107 84
AseI AT'TA_AT
Cuts at: 160 1032 4213 160
Size: 872 3181 1606
Fragments arranged by size:
3181 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
AvaI C'yCGr_G
Cuts at: 1870 1943 2984 1870
Size: 73 1041 4545
Fragments arranged by size:
4545 1041 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2751 2784 2751
Size: 33 5626
BamHI G'GATC_C
Cuts at: 2892 2989 3356 2892
Size: 97 367 5195
Fragments arranged by size:
5195 367 97
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2018 2189 2980 4435 618
Size: 325 217 627 231 171 791 1455 1842
Fragments arranged by size:
1842 1455 791 627 325 231 217 171
BanII G_rGCy'C
Cuts at: 1391 2978 1391
Size: 1587 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2176 928
Size: 971 277 4411
Fragments arranged by size:
4411 971 277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3850 3884 1903
Size: 34 1913 34 3678
Fragments arranged by size:
3678 1913 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3551 4761 3551
Size: 1210 4449
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5659
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4270 136
Size: 107 122 71 631 605 2598 1525
Fragments arranged by size:
2598 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5654 5654
Size: 5659
BlpI GC'TnA_GC
Cuts at: 2492 3076 2492
Size: 584 5075
Bme1580I G_kGCm'C
Cuts at: 1790 2194 3720 4876 1790
Size: 404 1526 1156 2573
Fragments arranged by size:
2573 1526 1156 404
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5659
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2602 2726 3390
Size: 416 53 83 399 24 1185 124 664
Cuts at: 3390 4348 442
Size: 958 1753
Fragments arranged by size:
1753 1185 958 664 416 399 124 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5659
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2933 2933
Size: 5659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2162 2736 4319 1239
Size: 923 574 1583 2579
Fragments arranged by size:
2579 1583 923 574
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2459 3784 4649 4947 2459
Size: 1325 865 298 3171
Fragments arranged by size:
3171 1325 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2473 4322 882
Size: 617 974 1849 2219
Fragments arranged by size:
2219 1849 974 617
BsaAI yAC'GTr
Cuts at: 493 1204 3450 493
Size: 711 2246 2702
Fragments arranged by size:
2702 2246 711
BsaBI GATnn'nnATC
Cuts at: 5653 5653
Size: 5659
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2293 2802 3466 3848
Size: 53 83 186 936 760 509 664 382
Cuts at: 3848 4483 275
Size: 635 1451
Fragments arranged by size:
1451 936 760 664 635 509 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2010 4092 4490 4764 4911 5322 2010
Size: 2082 398 274 147 411 2347
Fragments arranged by size:
2347 2082 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5434
Fragments arranged by size:
5434 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2165 1186
Size: 979 4680
BseYI C'CCAG_C
Cuts at: 2193 4862 5358 2193
Size: 2669 496 2494
Fragments arranged by size:
2669 2494 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2621 1163
Size: 573 885 4201
Fragments arranged by size:
4201 885 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3109 3870 4898 1540
Size: 275 186 1108 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3720 3805 4876 3720
Size: 85 1071 4503
Fragments arranged by size:
4503 1071 85
BsmI GAATG_Cn'
Cuts at: 3170 3263 3170
Size: 93 5566
BsmBI CGTCTCn'nnnn_
Cuts at: 2284 2284
Size: 5659
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2194 2978 3720 3805 4876 1391
Size: 399 404 784 742 85 1071 2174
Fragments arranged by size:
2174 1071 784 742 404 399 85
BspEI T'CCGG_A
Cuts at: 5322 5322
Size: 5659
BspHI T'CATG_A
Cuts at: 3548 5278 3548
Size: 1730 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3014 844
Size: 2170 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2884 3546 1394
Size: 429 1061 662 3507
Fragments arranged by size:
3507 1061 662 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2080 2732 4154 4328 65
Size: 1183 832 652 1422 174 1396
Fragments arranged by size:
1422 1396 1183 832 652 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2010 4303 4490 1700
Size: 110 200 2293 187 2869
Fragments arranged by size:
2869 2293 200 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5659
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5659
BssSI C'ACGA_G
Cuts at: 3719 4731 3719
Size: 1012 4647
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5659
BstBI TT'CG_AA
Cuts at: 2344 4463 2344
Size: 2119 3540
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5659
BstXI CCAn_nnnn'nTGG
Cuts at: 2186 2186
Size: 5659
BstYI r'GATC_y
Cuts at: 1972 2892 2989 3356 3741 3758 5210 5296 5308
Size: 920 97 367 385 17 1452 86 12
Cuts at: 5308 5654 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 920 385 367 346 97 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2037 2952 513
Size: 552 972 915 3220
Fragments arranged by size:
3220 972 915 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2230 487
Size: 683 56 1004 3916
Fragments arranged by size:
3916 1004 683 56
BtsI GCAGTG_nn'
Cuts at: 2727 3175 3969 3989 2727
Size: 448 794 20 4397
Fragments arranged by size:
4397 794 448 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5624
DraI TTT'AAA
Cuts at: 2963 2963
Size: 5659
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4666 809
Size: 507 3350 1802
Fragments arranged by size:
3350 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3106 3995 5624
Size: 54 1425 50 275 18 1276 889 1629
Cuts at: 5624 8
Size: 43
Fragments arranged by size:
1629 1425 1276 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3106 1537
Size: 275 1294 4090
Fragments arranged by size:
4090 1294 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2266 2581 3589 1353
Size: 913 315 1008 3423
Fragments arranged by size:
3423 1008 913 315
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2680 4246 4620 4766 387
Size: 1064 138 1091 1566 374 146 1280
Fragments arranged by size:
1566 1280 1091 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 2615 2736 2918 3722 4319 5106
Size: 393 530 453 121 182 804 597 787
Cuts at: 5106 5374 1239
Size: 268 1524
Fragments arranged by size:
1524 804 787 597 530 453 393 268
182 121
EcoRI G'AATT_C
Cuts at: 2233 2340 2968 3346 2233
Size: 107 628 378 4546
Fragments arranged by size:
4546 628 378 107
EcoRV GAT'ATC
Cuts at: 2113 2113
Size: 5659
FspI TGC'GCA
Cuts at: 4165 4165
Size: 5659
HaeII r_GCGC'y
Cuts at: 1678 4806 1678
Size: 3128 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2208 2240 2641 2673 4300
Size: 32 135 32 636 32 401 32 1627
Cuts at: 4300 4332 4374 4406 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1627 636 401 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3003 3249 669
Size: 988 281 1065 246 3079
Fragments arranged by size:
3079 1065 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5659
HpaI GTT'AAC
Cuts at: 3249 3249
Size: 5659
KpnI G_GTAC'C
Cuts at: 2022 2984 2022
Size: 962 4697
MfeI C'AATT_G
Cuts at: 3258 3258
Size: 5659
MluI A'CGCG_T
Cuts at: 5535 5535
Size: 5659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2251 2833 4773 4957 1305
Size: 946 582 1940 184 2007
Fragments arranged by size:
2007 1940 946 582 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3837
Fragments arranged by size:
3837 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3617 3976 4135 518
Size: 648 2451 359 159 2042
Fragments arranged by size:
2451 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3075 3752 4900 5145 1606
Size: 1469 677 1148 245 2120
Fragments arranged by size:
2120 1469 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5107
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5659
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5659
NotI GC'GGCC_GC
Cuts at: 3106 3106
Size: 5659
NspI r_CATG'y
Cuts at: 1132 1229 3017 4562 1132
Size: 97 1788 1545 2229
Fragments arranged by size:
2229 1788 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5659
PciI A'CATG_T
Cuts at: 4558 4558
Size: 5659
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5659
PfoI T'CCnGG_A
Cuts at: 2713 2713
Size: 5659
PmeI GTTT'AAAC
Cuts at: 2963 2963
Size: 5659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 5241 5273 3728
Size: 32 1481 32 4114
Fragments arranged by size:
4114 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5659
PsiI TTA'TAA
Cuts at: 3229 3229
Size: 5659
PstI C_TGCA'G
Cuts at: 830 2835 3011 830
Size: 2005 176 3478
Fragments arranged by size:
3478 2005 176
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5659
SalI G'TCGA_C
Cuts at: 1936 3001 1936
Size: 1065 4594
SbfI CC_TGCA'GG
Cuts at: 3011 3011
Size: 5659
ScaI AGT'ACT
Cuts at: 3907 5619 3907
Size: 1712 3947
SfcI C'TryA_G
Cuts at: 826 1045 2831 3007 4142 4823 5014 826
Size: 219 1786 176 1135 681 191 1471
Fragments arranged by size:
1786 1471 1135 681 219 191 176
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5659
SmaI CCC'GGG
Cuts at: 1872 2986 1872
Size: 1114 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2474 3763 4664 4926 820
Size: 197 926 531 1289 901 262 1553
Fragments arranged by size:
1553 1289 926 901 531 262 197
SnaBI TAC'GTA
Cuts at: 493 3450 493
Size: 2957 2702
SpeI A'CTAG_T
Cuts at: 152 2252 152
Size: 2100 3559
SphI G_CATG'C
Cuts at: 1132 3017 1132
Size: 1885 3774
SspI AAT'ATT
Cuts at: 5 52 2402 3583 5
Size: 47 2350 1181 2081
Fragments arranged by size:
2350 2081 1181 47
StuI AGG'CCT
Cuts at: 1517 2592 1517
Size: 1075 4584
StyI C'CwwG_G
Cuts at: 513 1065 3098 513
Size: 552 2033 3074
Fragments arranged by size:
3074 2033 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3686 3703 3856 4041 4486 325
Size: 1255 2106 17 153 185 445 1498
Fragments arranged by size:
2106 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3905 5617 96
Size: 274 80 33 51 1443 1928 1712 138
Fragments arranged by size:
1928 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5619 5651 5619
Size: 32 5627
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5659
XbaI T'CTAG_A
Cuts at: 2995 2995
Size: 5659
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2234 2234
Size: 5659
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5659
XmaI C'CCGG_G
Cuts at: 1870 2984 1870
Size: 1114 4545
XmnI GAAnn'nnTTC
Cuts at: 3788 3788
Size: 5659
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2294 2803 3467 276
Size: 53 83 186 936 760 509 664 2468
Fragments arranged by size:
2468 936 760 664 509 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI