Restriction map for
pFN21AE0105
(Circular) (Six-base) MAPSORT of: pFN21AE0105.seq Check: 5696 from: 1 to: 5248
pFN21AE0105 5248 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3058 278
Size: 53 83 186 936 1522 2468
Fragments arranged by size:
2468 1522 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2591 1937
Size: 654 4594
Acc65I G'GTAC_C
Cuts at: 2569 2569
Size: 5248
AclI AA'CG_TT
Cuts at: 1563 3375 3748 1563
Size: 1812 373 3063
Fragments arranged by size:
3063 1812 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3311 4695 4963 1632
Size: 1679 1384 268 1917
Fragments arranged by size:
1917 1679 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5051
AflIII A'CryG_T
Cuts at: 1203 4147 5124 1203
Size: 2944 977 1327
Fragments arranged by size:
2944 1327 977
AgeI A'CCGG_T
Cuts at: 4079 4079
Size: 5248
AhdI GACnn_n'nnGTC
Cuts at: 3977 3977
Size: 5248
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5248
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5248
AlwNI CAG_nnn'CTG
Cuts at: 2991 4563 2991
Size: 1572 3676
ApaLI G'TGCA_C
Cuts at: 3305 4461 3305
Size: 1156 4092
ApoI r'AATT_y
Cuts at: 1493 2436 2557 2783 2935 5044 1493
Size: 943 121 226 152 2109 1697
Fragments arranged by size:
2109 1697 943 226 152 121
AseI AT'TA_AT
Cuts at: 160 1032 3802 160
Size: 872 2770 1606
Fragments arranged by size:
2770 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5248
AvaI C'yCGr_G
Cuts at: 1870 1943 2573 1870
Size: 73 630 4545
Fragments arranged by size:
4545 630 73
BamHI G'GATC_C
Cuts at: 2578 2945 2578
Size: 367 4881
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2501 2569 4024 618
Size: 325 217 627 714 68 1455 1842
Fragments arranged by size:
1842 1455 714 627 325 217 68
BanII G_rGCy'C
Cuts at: 1391 2126 2567 1391
Size: 735 441 4072
Fragments arranged by size:
4072 735 441
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2544 928
Size: 971 645 3632
Fragments arranged by size:
3632 971 645
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3439 3473 1903
Size: 34 1502 34 3678
Fragments arranged by size:
3678 1502 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3140 4350 3140
Size: 1210 4038
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5248
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3859 136
Size: 107 122 71 631 605 2187 1525
Fragments arranged by size:
2187 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5243 5243
Size: 5248
BlpI GC'TnA_GC
Cuts at: 2120 2222 2665 2120
Size: 102 443 4703
Fragments arranged by size:
4703 443 102
Bme1580I G_kGCm'C
Cuts at: 1790 3309 4465 1790
Size: 1519 1156 2573
Fragments arranged by size:
2573 1519 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5248
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2464 2979 3937
Size: 416 53 83 399 24 1047 515 958
Cuts at: 3937 442
Size: 1753
Fragments arranged by size:
1753 1047 958 515 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5248
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3908 1239
Size: 2669 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3373 4238 4536 3373
Size: 865 298 4085
Fragments arranged by size:
4085 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3911 882
Size: 617 2412 2219
Fragments arranged by size:
2412 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3039 493
Size: 711 1835 2702
Fragments arranged by size:
2702 1835 711
BsaBI GATnn'nnATC
Cuts at: 2309 5242 2309
Size: 2933 2315
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3055 3437 4072 275
Size: 53 83 186 936 1522 382 635 1451
Fragments arranged by size:
1522 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3681 4079 4353 4500 4911 3681
Size: 398 274 147 411 4018
Fragments arranged by size:
4018 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5023
Fragments arranged by size:
5023 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 1186
Size: 859 4389
BseYI C'CCAG_C
Cuts at: 4451 4947 4451
Size: 496 4752
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4675
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2698 3459 4487 1540
Size: 275 186 697 761 1028 2301
Fragments arranged by size:
2301 1028 761 697 275 186
BsiHKAI G_wGCw'C
Cuts at: 3309 3394 4465 3309
Size: 85 1071 4092
Fragments arranged by size:
4092 1071 85
BsmI GAATG_Cn'
Cuts at: 2288 2759 2852 2288
Size: 471 93 4684
Fragments arranged by size:
4684 471 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2126 2567 3309 3394 4465 1391
Size: 399 336 441 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 441 399 336 85
BspEI T'CCGG_A
Cuts at: 4911 4911
Size: 5248
BspHI T'CATG_A
Cuts at: 3137 4867 3137
Size: 1730 3518
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2603 844
Size: 1759 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3135 1394
Size: 429 1312 3507
Fragments arranged by size:
3507 1312 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2283 3743 3917 65
Size: 1183 1035 1460 174 1396
Fragments arranged by size:
1460 1396 1183 1035 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3892 4079 1700
Size: 110 2082 187 2869
Fragments arranged by size:
2869 2082 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5248
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5248
BssSI C'ACGA_G
Cuts at: 3308 4320 3308
Size: 1012 4236
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5248
BstBI TT'CG_AA
Cuts at: 4052 4052
Size: 5248
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5248
BstYI r'GATC_y
Cuts at: 1972 2321 2578 2945 3330 3347 4799 4885 4897
Size: 349 257 367 385 17 1452 86 12
Cuts at: 4897 5243 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 349 346 257 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3757
Fragments arranged by size:
3757 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4509
Fragments arranged by size:
4509 683 56
BtsI GCAGTG_nn'
Cuts at: 2206 2764 3558 3578 2206
Size: 558 794 20 3876
Fragments arranged by size:
3876 794 558 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5248
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5213
DraI TTT'AAA
Cuts at: 2552 2552
Size: 5248
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4255 809
Size: 507 2939 1802
Fragments arranged by size:
2939 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2695 3584 5213
Size: 54 1425 50 275 18 865 889 1629
Cuts at: 5213 8
Size: 43
Fragments arranged by size:
1629 1425 889 865 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2695 1537
Size: 275 883 4090
Fragments arranged by size:
4090 883 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3178 1353
Size: 1825 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3835 4209 4355 387
Size: 1064 138 2246 374 146 1280
Fragments arranged by size:
2246 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3311 3908 4695 4963 1239
Size: 393 1679 597 787 268 1524
Fragments arranged by size:
1679 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2557 2935 2557
Size: 378 4870
FspI TGC'GCA
Cuts at: 3754 3754
Size: 5248
HaeII r_GCGC'y
Cuts at: 1678 2096 2271 4395 1678
Size: 418 175 2124 2531
Fragments arranged by size:
2531 2124 418 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2230 2262 3889 3921 3963
Size: 32 135 32 658 32 1627 32 42
Cuts at: 3963 3995 1373
Size: 32 2626
Fragments arranged by size:
2626 1627 658 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2592 2838 669
Size: 988 281 654 246 3079
Fragments arranged by size:
3079 988 654 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5248
HpaI GTT'AAC
Cuts at: 2838 2838
Size: 5248
KpnI G_GTAC'C
Cuts at: 2573 2573
Size: 5248
MfeI C'AATT_G
Cuts at: 2847 2847
Size: 5248
MluI A'CGCG_T
Cuts at: 5124 5124
Size: 5248
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4362 4546 1305
Size: 3057 184 2007
Fragments arranged by size:
3057 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3426
Fragments arranged by size:
3426 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3206 3565 3724 518
Size: 648 2040 359 159 2042
Fragments arranged by size:
2042 2040 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2022 2141 2664 3341 4489 4734 1606
Size: 416 119 523 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 523 416 245 119
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3757
Fragments arranged by size:
3757 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5248
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5248
NotI GC'GGCC_GC
Cuts at: 2695 2695
Size: 5248
NspI r_CATG'y
Cuts at: 1132 1229 2606 4151 1132
Size: 97 1377 1545 2229
Fragments arranged by size:
2229 1545 1377 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5248
PciI A'CATG_T
Cuts at: 4147 4147
Size: 5248
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5248
PmeI GTTT'AAAC
Cuts at: 2552 2552
Size: 5248
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3317 3349 4830 4862 3317
Size: 32 1481 32 3703
Fragments arranged by size:
3703 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5248
PsiI TTA'TAA
Cuts at: 2818 2818
Size: 5248
PstI C_TGCA'G
Cuts at: 830 2600 830
Size: 1770 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5248
PvuII CAG'CTG
Cuts at: 2141 2141
Size: 5248
SalI G'TCGA_C
Cuts at: 1936 2590 1936
Size: 654 4594
SbfI CC_TGCA'GG
Cuts at: 2600 2600
Size: 5248
ScaI AGT'ACT
Cuts at: 2153 3496 5208 2153
Size: 1343 1712 2193
Fragments arranged by size:
2193 1712 1343
SfcI C'TryA_G
Cuts at: 826 1045 2338 2596 3731 4412 4603 826
Size: 219 1293 258 1135 681 191 1471
Fragments arranged by size:
1471 1293 1135 681 258 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5248
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5248
SmaI CCC'GGG
Cuts at: 1872 2575 1872
Size: 703 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3352 4253 4515 820
Size: 197 926 1409 901 262 1553
Fragments arranged by size:
1553 1409 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3039 493
Size: 2546 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5248
SphI G_CATG'C
Cuts at: 1132 2606 1132
Size: 1474 3774
SspI AAT'ATT
Cuts at: 5 52 3172 5
Size: 47 3120 2081
Fragments arranged by size:
3120 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5248
StyI C'CwwG_G
Cuts at: 513 1065 2004 2687 513
Size: 552 939 683 3074
Fragments arranged by size:
3074 939 683 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3275 3292 3445 3630 4075 325
Size: 1255 1695 17 153 185 445 1498
Fragments arranged by size:
1695 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2151 3494 5206
Size: 274 80 33 51 1443 174 1343 1712
Cuts at: 5206 96
Size: 138
Fragments arranged by size:
1712 1443 1343 274 174 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2198 2230 5208 5240 2198
Size: 32 2978 32 2206
Fragments arranged by size:
2978 2206 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5248
XbaI T'CTAG_A
Cuts at: 2584 2584
Size: 5248
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5248
XmaI C'CCGG_G
Cuts at: 1870 2573 1870
Size: 703 4545
XmnI GAAnn'nnTTC
Cuts at: 3377 3377
Size: 5248
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3056 276
Size: 53 83 186 936 1522 2468
Fragments arranged by size:
2468 1522 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI