Restriction map for
pFN21AE0104
(Circular) (Six-base) MAPSORT of: pFN21AE0104.seq Check: 6192 from: 1 to: 6091
pFN21AE0104 6091 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3221 3434 1937
Size: 1284 213 4594
Fragments arranged by size:
4594 1284 213
Acc65I G'GTAC_C
Cuts at: 3412 3412
Size: 6091
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2048 2088 2736 4154 5538 5806 1632
Size: 416 40 648 1418 1384 268 1917
Fragments arranged by size:
1917 1418 1384 648 416 268 40
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 4990 5967 1203
Size: 3787 977 1327
Fragments arranged by size:
3787 1327 977
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6091
AlwNI CAG_nnn'CTG
Cuts at: 2066 3286 3834 5406 2066
Size: 1220 548 1572 2751
Fragments arranged by size:
2751 1572 1220 548
ApaLI G'TGCA_C
Cuts at: 4148 5304 4148
Size: 1156 4935
ApoI r'AATT_y
Cuts at: 1493 2720 3400 3626 3778 5887 1493
Size: 1227 680 226 152 2109 1697
Fragments arranged by size:
2109 1697 1227 680 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3356 4645 160
Size: 872 2324 1289 1606
Fragments arranged by size:
2324 1606 1289 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 2653 3416 1870
Size: 73 710 763 4545
Fragments arranged by size:
4545 763 710 73
BamHI G'GATC_C
Cuts at: 3421 3788 3421
Size: 367 5724
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2175 3412 4867 618
Size: 325 217 627 388 1237 1455 1842
Fragments arranged by size:
1842 1455 1237 627 388 325 217
BanII G_rGCy'C
Cuts at: 1391 3410 1391
Size: 2019 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2001 2925 928
Size: 971 102 924 4094
Fragments arranged by size:
4094 971 924 102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4282 4316 1903
Size: 34 2345 34 3678
Fragments arranged by size:
3678 2345 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3983 5193 3983
Size: 1210 4881
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3021 4702 136
Size: 107 122 71 631 605 1349 1681 1525
Fragments arranged by size:
1681 1525 1349 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2819 6086 2819
Size: 3267 2824
BlpI GC'TnA_GC
Cuts at: 3205 3508 3205
Size: 303 5788
Bme1580I G_kGCm'C
Cuts at: 1790 2178 4152 5308 1790
Size: 388 1974 1156 2573
Fragments arranged by size:
2573 1974 1156 388
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6091
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3822 4780 442
Size: 416 53 83 399 24 2405 958 1753
Fragments arranged by size:
2405 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2426 1056
Size: 1370 4721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3102 4751 1239
Size: 1863 1649 2579
Fragments arranged by size:
2579 1863 1649
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2222 2796 4216 5081 5379 2222
Size: 574 1420 865 298 2934
Fragments arranged by size:
2934 1420 865 574 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4754 882
Size: 617 3255 2219
Fragments arranged by size:
3255 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3882 493
Size: 711 2678 2702
Fragments arranged by size:
2702 2678 711
BsaBI GATnn'nnATC
Cuts at: 2472 6085 2472
Size: 3613 2478
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2940 3898 4280 4915
Size: 53 83 186 936 1407 958 382 635
Cuts at: 4915 275
Size: 1451
Fragments arranged by size:
1451 1407 958 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2385 4524 4922 5196 5343 5754 2385
Size: 2139 398 274 147 411 2722
Fragments arranged by size:
2722 2139 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3123 3153 1178
Size: 30 165 30 1720 30 4116
Fragments arranged by size:
4116 1720 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2675 1186
Size: 1489 4602
BseYI C'CCAG_C
Cuts at: 2571 2798 5294 5790 2571
Size: 227 2496 496 2872
Fragments arranged by size:
2872 2496 496 227
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2232 1163
Size: 573 496 5022
Fragments arranged by size:
5022 573 496
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3541 4302 5330 1540
Size: 275 186 1540 761 1028 2301
Fragments arranged by size:
2301 1540 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4152 4237 5308 4152
Size: 85 1071 4935
Fragments arranged by size:
4935 1071 85
BsmI GAATG_Cn'
Cuts at: 3317 3602 3695 3317
Size: 285 93 5713
Fragments arranged by size:
5713 285 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2178 3410 4152 4237 5308 1391
Size: 399 388 1232 742 85 1071 2174
Fragments arranged by size:
2174 1232 1071 742 399 388 85
BspEI T'CCGG_A
Cuts at: 5754 5754
Size: 6091
BspHI T'CATG_A
Cuts at: 3980 5710 3980
Size: 1730 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3446 844
Size: 2602 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3978 1394
Size: 429 2155 3507
Fragments arranged by size:
3507 2155 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4586 4760 65
Size: 1183 3338 174 1396
Fragments arranged by size:
3338 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4735 4922 1700
Size: 110 2925 187 2869
Fragments arranged by size:
2925 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6091
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 4151 5163 4151
Size: 1012 5079
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6091
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6091
BstYI r'GATC_y
Cuts at: 1972 2701 2819 3421 3788 4173 4190 5642 5728
Size: 729 118 602 367 385 17 1452 86
Cuts at: 5728 5740 6086 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 729 602 385 367 346 118
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3170 513
Size: 552 2105 3434
Fragments arranged by size:
3434 2105 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5352
Fragments arranged by size:
5352 683 56
BtsI GCAGTG_nn'
Cuts at: 3222 3607 4401 4421 3222
Size: 385 794 20 4892
Fragments arranged by size:
4892 794 385 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 2472 2913 3395 2472
Size: 441 482 5168
Fragments arranged by size:
5168 482 441
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5098 809
Size: 507 3782 1802
Fragments arranged by size:
3782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3142 3173 3538
Size: 54 1425 50 275 18 1312 31 365
Cuts at: 3538 4427 6056 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1312 889 365 275 54 50
43 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 3538 1537
Size: 275 1726 4090
Fragments arranged by size:
4090 1726 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2121 3114 4021 1353
Size: 768 993 907 3423
Fragments arranged by size:
3423 993 907 768
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4678 5052 5198 387
Size: 1064 138 3089 374 146 1280
Fragments arranged by size:
3089 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2088 2736 3102 4154 4751 5538
Size: 393 416 40 648 366 1052 597 787
Cuts at: 5538 5806 1239
Size: 268 1524
Fragments arranged by size:
1524 1052 787 648 597 416 393 366
268 40
EcoO109I rG'GnC_Cy
Cuts at: 3283 3283
Size: 6091
EcoRI G'AATT_C
Cuts at: 2720 3400 3778 2720
Size: 680 378 5033
Fragments arranged by size:
5033 680 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2546 2605 2546
Size: 59 6032
FspI TGC'GCA
Cuts at: 4597 4597
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 5238 1678
Size: 3560 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2211 2243 3267 3299 4732
Size: 32 135 32 639 32 1024 32 1433
Cuts at: 4732 4764 4806 4838 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1433 1024 639 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2088 3167 3238 3435 3681 669
Size: 988 281 150 1079 71 197 246 3079
Fragments arranged by size:
3079 1079 988 281 246 197 150 71
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6091
HpaI GTT'AAC
Cuts at: 2088 3681 2088
Size: 1593 4498
KpnI G_GTAC'C
Cuts at: 3416 3416
Size: 6091
MfeI C'AATT_G
Cuts at: 2223 2780 3690 2223
Size: 557 910 4624
Fragments arranged by size:
4624 910 557
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2536 5205 5389 1305
Size: 1231 2669 184 2007
Fragments arranged by size:
2669 2007 1231 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3144 3175 10
Size: 54 1425 343 1312 31 2926
Fragments arranged by size:
2926 1425 1312 343 54 31
MslI CAynn'nnrTG
Cuts at: 518 1166 3000 4049 4408 4567 518
Size: 648 1834 1049 359 159 2042
Fragments arranged by size:
2042 1834 1049 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2063 2539 3264 3507 4184 5332 5577 1606
Size: 457 476 725 243 677 1148 245 2120
Fragments arranged by size:
2120 1148 725 677 476 457 245 243
NcoI C'CATG_G
Cuts at: 513 1065 3170 513
Size: 552 2105 3434
Fragments arranged by size:
3434 2105 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 2422 1052
Size: 1370 4721
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 3052 3449 4994 1132
Size: 97 1823 397 1545 2229
Fragments arranged by size:
2229 1823 1545 397 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6091
PciI A'CATG_T
Cuts at: 4990 4990
Size: 6091
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2864 1108
Size: 1756 4335
PfoI T'CCnGG_A
Cuts at: 3285 3285
Size: 6091
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3283 3283
Size: 6091
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6091
PsiI TTA'TAA
Cuts at: 2277 3661 2277
Size: 1384 4707
PstI C_TGCA'G
Cuts at: 830 3443 830
Size: 2613 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 2063 2539 2063
Size: 476 5615
SalI G'TCGA_C
Cuts at: 1936 3433 1936
Size: 1497 4594
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 4339 6051 4339
Size: 1712 4379
SfcI C'TryA_G
Cuts at: 826 1045 3222 3439 4574 5255 5446 826
Size: 219 2177 217 1135 681 191 1471
Fragments arranged by size:
2177 1471 1135 681 219 217 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 3418 1872
Size: 1546 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2201 2811 4195 5096 5358 820
Size: 197 926 258 610 1384 901 262 1553
Fragments arranged by size:
1553 1384 926 901 610 262 258 197
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 3449 1132
Size: 2317 3774
SspI AAT'ATT
Cuts at: 5 52 4015 5
Size: 47 3963 2081
Fragments arranged by size:
3963 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6091
StyI C'CwwG_G
Cuts at: 513 1065 2324 2478 3170 3530 513
Size: 552 1259 154 692 360 3074
Fragments arranged by size:
3074 1259 692 552 360 154
SwaI ATTT'AAAT
Cuts at: 2472 2472
Size: 6091
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2193 4118 4135 4288 4473 4918 325
Size: 1255 613 1925 17 153 185 445 1498
Fragments arranged by size:
1925 1498 1255 613 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4337 6049 96
Size: 274 80 33 51 1443 2360 1712 138
Fragments arranged by size:
2360 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6051 6083 6051
Size: 32 6059
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6091
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 3416 1870
Size: 1546 4545
XmnI GAAnn'nnTTC
Cuts at: 4220 4220
Size: 6091
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI XcmI