Restriction map for
pFN21AE0103
(Circular) (Six-base) MAPSORT of: pFN21AE0103.seq Check: 3871 from: 1 to: 5905
pFN21AE0103 5905 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2407 2407
Size: 5905
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3715 278
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2604 3248 1937
Size: 667 644 4594
Fragments arranged by size:
4594 667 644
Acc65I G'GTAC_C
Cuts at: 3226 3226
Size: 5905
AclI AA'CG_TT
Cuts at: 1563 4032 4405 1563
Size: 2469 373 3063
Fragments arranged by size:
3063 2469 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3968 5352 5620 1632
Size: 2336 1384 268 1917
Fragments arranged by size:
2336 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 2044 4804 5781 1203
Size: 841 2760 977 1327
Fragments arranged by size:
2760 1327 977 841
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 4634 4634
Size: 5905
AleI CACnn'nnGTG
Cuts at: 1166 3002 1166
Size: 1836 4069
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5905
AlwNI CAG_nnn'CTG
Cuts at: 3648 5220 3648
Size: 1572 4333
ApaI G_GGCC'C
Cuts at: 2774 3133 2774
Size: 359 5546
ApaLI G'TGCA_C
Cuts at: 2270 3962 5118 2270
Size: 1692 1156 3057
Fragments arranged by size:
3057 1692 1156
ApoI r'AATT_y
Cuts at: 1493 3214 3440 3592 5701 1493
Size: 1721 226 152 2109 1697
Fragments arranged by size:
2109 1721 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4459 160
Size: 872 3427 1606
Fragments arranged by size:
3427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 2101 3230 1870
Size: 73 158 1129 4545
Fragments arranged by size:
4545 1129 158 73
BamHI G'GATC_C
Cuts at: 2988 3235 3602 2988
Size: 247 367 5291
Fragments arranged by size:
5291 367 247
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2282 3184 3226 4681 618
Size: 325 217 627 495 902 42 1455 1842
Fragments arranged by size:
1842 1455 902 627 495 325 217 42
BanII G_rGCy'C
Cuts at: 1391 2124 2208 2774 3133 3224 1391
Size: 733 84 566 359 91 4072
Fragments arranged by size:
4072 733 566 359 91 84
BbeI G_GCGC'C
Cuts at: 2286 2286
Size: 5905
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2186 2930 928
Size: 971 287 744 3903
Fragments arranged by size:
3903 971 744 287
BbvCI CC'TCA_GC
Cuts at: 2199 2199
Size: 5905
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2467 2501 4096 4130 1903
Size: 34 530 34 1595 34 3678
Fragments arranged by size:
3678 1595 530 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2712 2839 3797 5007 2712
Size: 127 958 1210 3610
Fragments arranged by size:
3610 1210 958 127
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5905
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3149 4516 136
Size: 107 122 71 631 605 1477 1367 1525
Fragments arranged by size:
1525 1477 1367 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5900 5900
Size: 5905
BlpI GC'TnA_GC
Cuts at: 2090 3322 2090
Size: 1232 4673
Bme1580I G_kGCm'C
Cuts at: 1790 2274 2774 3133 3162 3966 5122 1790
Size: 484 500 359 29 804 1156 2573
Fragments arranged by size:
2573 1156 804 500 484 359 29
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5905
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2508 3636 4594
Size: 416 53 83 399 24 1091 1128 958
Cuts at: 4594 442
Size: 1753
Fragments arranged by size:
1753 1128 1091 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5905
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 5905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2657 4565 1239
Size: 1418 1908 2579
Fragments arranged by size:
2579 1908 1418
Bpu10I CC'TnA_GC
Cuts at: 2199 2621 2199
Size: 422 5483
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4030 4895 5193 4030
Size: 865 298 4742
Fragments arranged by size:
4742 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2090 3021 4568 882
Size: 617 591 931 1547 2219
Fragments arranged by size:
2219 1547 931 617 591
BsaAI yAC'GTr
Cuts at: 493 1204 2359 2782 3696 493
Size: 711 1155 423 914 2702
Fragments arranged by size:
2702 1155 914 711 423
BsaBI GATnn'nnATC
Cuts at: 5899 5899
Size: 5905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2283 3712 4094 4729
Size: 53 83 186 936 750 1429 382 635
Cuts at: 4729 275
Size: 1451
Fragments arranged by size:
1451 1429 936 750 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2248 4338 4736 5010 5157 5568 2248
Size: 2090 398 274 147 411 2585
Fragments arranged by size:
2585 2090 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2420 2450 1178
Size: 30 165 30 1017 30 4633
Fragments arranged by size:
4633 1017 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2402 2900 3125 1186
Size: 1216 498 225 3966
Fragments arranged by size:
3966 1216 498 225
BseYI C'CCAG_C
Cuts at: 2067 2773 5108 5604 2067
Size: 706 2335 496 2368
Fragments arranged by size:
2368 2335 706 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2846 1163
Size: 573 1110 4222
Fragments arranged by size:
4222 1110 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2491 3355 4116 5144 1540
Size: 275 186 490 864 761 1028 2301
Fragments arranged by size:
2301 1028 864 761 490 275 186
BsiHKAI G_wGCw'C
Cuts at: 2274 3966 4051 5122 2274
Size: 1692 85 1071 3057
Fragments arranged by size:
3057 1692 1071 85
BsmI GAATG_Cn'
Cuts at: 3416 3509 3416
Size: 93 5812
BsmBI CGTCTCn'nnnn_
Cuts at: 2423 2423
Size: 5905
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2208 2274 2774 3133 3162 3224
Size: 399 334 84 66 500 359 29 62
Cuts at: 3224 3966 4051 5122 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 500 399 359 334 85
84 66 62 29
BspEI T'CCGG_A
Cuts at: 2248 5568 2248
Size: 3320 2585
BspHI T'CATG_A
Cuts at: 3794 5524 3794
Size: 1730 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2407 2559 3260 844
Size: 1563 152 701 3489
Fragments arranged by size:
3489 1563 701 152
BsrBI CCG'CTC
Cuts at: 1394 1823 2751 3792 1394
Size: 429 928 1041 3507
Fragments arranged by size:
3507 1041 928 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3138 4400 4574 65
Size: 1183 1890 1262 174 1396
Fragments arranged by size:
1890 1396 1262 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3143 4549 4736 1700
Size: 110 1333 1406 187 2869
Fragments arranged by size:
2869 1406 1333 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5905
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5905
BssSI C'ACGA_G
Cuts at: 3965 4977 3965
Size: 1012 4893
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5905
BstBI TT'CG_AA
Cuts at: 4709 4709
Size: 5905
BstEII G'GTnAC_C
Cuts at: 1184 2810 1184
Size: 1626 4279
BstXI CCAn_nnnn'nTGG
Cuts at: 2521 2527 2965 3199 2521
Size: 6 438 234 5227
Fragments arranged by size:
5227 438 234 6
BstYI r'GATC_y
Cuts at: 1972 2988 3235 3602 3987 4004 5456 5542 5554
Size: 1016 247 367 385 17 1452 86 12
Cuts at: 5554 5900 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1016 385 367 346 247 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2520 2665 2866 3093 513
Size: 552 939 516 145 201 227 3325
Fragments arranged by size:
3325 939 552 516 227 201 145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2769 487
Size: 683 56 1543 3623
Fragments arranged by size:
3623 1543 683 56
BtsI GCAGTG_nn'
Cuts at: 2532 3421 4215 4235 2532
Size: 889 794 20 4202
Fragments arranged by size:
4202 889 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5870
DraI TTT'AAA
Cuts at: 3209 3209
Size: 5905
DraIII CAC_nnn'GTG
Cuts at: 2242 2242
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2723 2819 4912 809
Size: 507 1407 96 2093 1802
Fragments arranged by size:
2093 1802 1407 507 96
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2500 2955
Size: 54 1425 50 275 18 177 493 455
Cuts at: 2955 3069 3352 4241 5870 8
Size: 114 283 889 1629 43
Fragments arranged by size:
1629 1425 889 493 455 283 275 177
114 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3352 1537
Size: 275 1540 4090
Fragments arranged by size:
4090 1540 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3116 3835 1353
Size: 1763 719 3423
Fragments arranged by size:
3423 1763 719
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4492 4866 5012 387
Size: 1064 138 2903 374 146 1280
Fragments arranged by size:
2903 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2657 3968 4565 5352 5620 1239
Size: 393 1025 1311 597 787 268 1524
Fragments arranged by size:
1524 1311 1025 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2641 2932 3130 2641
Size: 291 198 5416
Fragments arranged by size:
5416 291 198
EcoRI G'AATT_C
Cuts at: 3214 3592 3214
Size: 378 5527
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 2286 5052 1678
Size: 608 2766 2531
Fragments arranged by size:
2766 2531 608
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2031 2063 3018 3050 4546
Size: 32 135 32 459 32 955 32 1496
Cuts at: 4546 4578 4620 4652 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1496 955 459 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2605 3037 3249 3495 669
Size: 988 281 667 432 212 246 3079
Fragments arranged by size:
3079 988 667 432 281 246 212
HindIII A'AGCT_T
Cuts at: 748 2300 748
Size: 1552 4353
HpaI GTT'AAC
Cuts at: 3495 3495
Size: 5905
KasI G'GCGC_C
Cuts at: 2282 2282
Size: 5905
KpnI G_GTAC'C
Cuts at: 3230 3230
Size: 5905
MfeI C'AATT_G
Cuts at: 3504 3504
Size: 5905
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3180 5019 5203 1305
Size: 1875 1839 184 2007
Fragments arranged by size:
2007 1875 1839 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2502 2957 3071 10
Size: 54 1425 343 670 455 114 2844
Fragments arranged by size:
2844 1425 670 455 343 114 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2525 2963 3002 3863 4222 4381 518
Size: 648 1359 438 39 861 359 159 2042
Fragments arranged by size:
2042 1359 861 648 438 359 159 39
MspA1I CmG'CkG
Cuts at: 1606 2806 2945 3321 3998 5146 5391 1606
Size: 1200 139 376 677 1148 245 2120
Fragments arranged by size:
2120 1200 1148 677 376 245 139
NaeI GCC'GGC
Cuts at: 3145 3145
Size: 5905
NarI GG'CG_CC
Cuts at: 2283 2283
Size: 5905
NcoI C'CATG_G
Cuts at: 513 1065 2004 2520 513
Size: 552 939 516 3898
Fragments arranged by size:
3898 939 552 516
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5905
NgoMIV G'CCGG_C
Cuts at: 3143 3143
Size: 5905
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5905
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 2048 3263 4808 1132
Size: 97 819 1215 1545 2229
Fragments arranged by size:
2229 1545 1215 819 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5905
PciI A'CATG_T
Cuts at: 2044 4804 2044
Size: 2760 3145
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2242 2321 1108
Size: 1134 79 4692
Fragments arranged by size:
4692 1134 79
PfoI T'CCnGG_A
Cuts at: 2079 2079
Size: 5905
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PmlI CAC'GTG
Cuts at: 2359 2782 2359
Size: 423 5482
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 5487 5519 3974
Size: 32 1481 32 4360
Fragments arranged by size:
4360 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2641 2641
Size: 5905
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5905
PsiI TTA'TAA
Cuts at: 3475 3475
Size: 5905
PspOMI G'GGCC_C
Cuts at: 2770 3129 2770
Size: 359 5546
PstI C_TGCA'G
Cuts at: 830 3257 830
Size: 2427 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5905
PvuII CAG'CTG
Cuts at: 2945 2945
Size: 5905
SalI G'TCGA_C
Cuts at: 1936 2603 3247 1936
Size: 667 644 4594
Fragments arranged by size:
4594 667 644
SapI GCTCTTCn'nnn_
Cuts at: 3116 3116
Size: 5905
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 4153 5865 4153
Size: 1712 4193
SexAI A'CCwGG_T
Cuts at: 3030 3030
Size: 5905
SfcI C'TryA_G
Cuts at: 826 1045 3253 4388 5069 5260 826
Size: 219 2208 1135 681 191 1471
Fragments arranged by size:
2208 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2284 2284
Size: 5905
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5905
SmaI CCC'GGG
Cuts at: 1872 3232 1872
Size: 1360 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4009 4910 5172 820
Size: 197 926 2066 901 262 1553
Fragments arranged by size:
2066 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 3263 1132
Size: 2131 3774
SspI AAT'ATT
Cuts at: 5 52 3829 5
Size: 47 3777 2081
Fragments arranged by size:
3777 2081 47
StuI AGG'CCT
Cuts at: 1517 2589 1517
Size: 1072 4833
StyI C'CwwG_G
Cuts at: 513 1065 2004 2520 3000 3344 513
Size: 552 939 516 480 344 3074
Fragments arranged by size:
3074 939 552 516 480 344
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2505 3932 3949 4102 4287 4732 325
Size: 1255 925 1427 17 153 185 445 1498
Fragments arranged by size:
1498 1427 1255 925 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2400 4151 5863
Size: 274 80 33 51 1443 423 1751 1712
Cuts at: 5863 96
Size: 138
Fragments arranged by size:
1751 1712 1443 423 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2253 2285 5865 5897 2253
Size: 32 3580 32 2261
Fragments arranged by size:
3580 2261 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2600 3032 1316
Size: 1284 432 4189
Fragments arranged by size:
4189 1284 432
XbaI T'CTAG_A
Cuts at: 3241 3241
Size: 5905
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5905
XmaI C'CCGG_G
Cuts at: 1870 3230 1870
Size: 1360 4545
XmnI GAAnn'nnTTC
Cuts at: 4034 4034
Size: 5905
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3713 276
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PspXI PsrI RsrII SacI SacII SanDI
SfiI SrfI SwaI XcmI