Restriction map for
pFN21AE0102
(Circular) (Six-base) MAPSORT of: pFN21AE0102.seq Check: 1209 from: 1 to: 5782
pFN21AE0102 5782 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3592 278
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3125 1937
Size: 1188 4594
Acc65I G'GTAC_C
Cuts at: 3103 3103
Size: 5782
AclI AA'CG_TT
Cuts at: 1563 3909 4282 1563
Size: 2346 373 3063
Fragments arranged by size:
3063 2346 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2468 3845 5229 5497 1632
Size: 836 1377 1384 268 1917
Fragments arranged by size:
1917 1384 1377 836 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5585
AflIII A'CryG_T
Cuts at: 1203 4681 5658 1203
Size: 3478 977 1327
Fragments arranged by size:
3478 1327 977
AgeI A'CCGG_T
Cuts at: 4613 4613
Size: 5782
AhdI GACnn_n'nnGTC
Cuts at: 4511 4511
Size: 5782
AleI CACnn'nnGTG
Cuts at: 1166 2396 1166
Size: 1230 4552
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5782
AlwNI CAG_nnn'CTG
Cuts at: 3055 3525 5097 3055
Size: 470 1572 3740
Fragments arranged by size:
3740 1572 470
ApaI G_GGCC'C
Cuts at: 2666 2666
Size: 5782
ApaLI G'TGCA_C
Cuts at: 3839 4995 3839
Size: 1156 4626
ApoI r'AATT_y
Cuts at: 1493 3091 3317 3469 5578 1493
Size: 1598 226 152 2109 1697
Fragments arranged by size:
2109 1697 1598 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4336 160
Size: 872 3304 1606
Fragments arranged by size:
3304 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
AvaI C'yCGr_G
Cuts at: 1870 1943 3107 1870
Size: 73 1164 4545
Fragments arranged by size:
4545 1164 73
BamHI G'GATC_C
Cuts at: 2931 3112 3479 2931
Size: 181 367 5234
Fragments arranged by size:
5234 367 181
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2276 2321 2360 2525
Size: 325 217 627 273 216 45 39 165
Cuts at: 2525 2603 2948 3103 4558 618
Size: 78 345 155 1455 1842
Fragments arranged by size:
1842 1455 627 345 325 273 217 216
165 155 78 45 39
BanII G_rGCy'C
Cuts at: 1391 2473 2666 3101 1391
Size: 1082 193 435 4072
Fragments arranged by size:
4072 1082 435 193
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4811
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3973 4007 1903
Size: 34 2036 34 3678
Fragments arranged by size:
3678 2036 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3674 4884 3674
Size: 1210 4572
BclI T'GATC_A
Cuts at: 1548 2538 3015 1548
Size: 990 477 4315
Fragments arranged by size:
4315 990 477
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4393 136
Size: 107 122 71 631 605 2721 1525
Fragments arranged by size:
2721 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5777 5777
Size: 5782
BlpI GC'TnA_GC
Cuts at: 2577 3199 2577
Size: 622 5160
Bme1580I G_kGCm'C
Cuts at: 1790 2270 2281 2363 2528 2666 2688 2951 3843
Size: 480 11 82 165 138 22 263 892
Cuts at: 3843 4999 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 892 480 263 165 138 82
22 11
BmgBI CAC'GTC
Cuts at: 1318 2104 2836 1318
Size: 786 732 4264
Fragments arranged by size:
4264 786 732
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3513 4471 442
Size: 416 53 83 399 24 2096 958 1753
Fragments arranged by size:
2096 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5782
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2024 2149 2270 2395 2570 4442 1239
Size: 785 125 121 125 175 1872 2579
Fragments arranged by size:
2579 1872 785 175 125 125 121
Bpu10I CC'TnA_GC
Cuts at: 2628 2628
Size: 5782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3907 4772 5070 3907
Size: 865 298 4619
Fragments arranged by size:
4619 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4445 882
Size: 617 2946 2219
Fragments arranged by size:
2946 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3573 493
Size: 711 2369 2702
Fragments arranged by size:
2702 2369 711
BsaBI GATnn'nnATC
Cuts at: 5776 5776
Size: 5782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3589 3971 4606 275
Size: 53 83 186 936 2056 382 635 1451
Fragments arranged by size:
2056 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4215 4613 4887 5034 5445 4215
Size: 398 274 147 411 4552
Fragments arranged by size:
4552 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2243 2273 1178
Size: 30 165 30 840 30 4687
Fragments arranged by size:
4687 840 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2191 1186
Size: 1005 4777
BseYI C'CCAG_C
Cuts at: 2599 2653 2658 4985 5481 2599
Size: 54 5 2327 496 2900
Fragments arranged by size:
2900 2327 496 54 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5209
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3232 3993 5021 1540
Size: 275 186 1231 761 1028 2301
Fragments arranged by size:
2301 1231 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3843 3928 4999 3843
Size: 85 1071 4626
Fragments arranged by size:
4626 1071 85
BsmI GAATG_Cn'
Cuts at: 3293 3386 3293
Size: 93 5689
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2270 2281 2363 2473 2528 2666 2688
Size: 399 480 11 82 110 55 138 22
Cuts at: 2688 2951 3101 3843 3928 4999 1391
Size: 263 150 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 480 399 263 150 138
110 85 82 55 22 11
BspEI T'CCGG_A
Cuts at: 5445 5445
Size: 5782
BspHI T'CATG_A
Cuts at: 2088 3012 3671 5401 2088
Size: 924 659 1730 2469
Fragments arranged by size:
2469 1730 924 659
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3137 844
Size: 2293 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2379 3669 1394
Size: 429 556 1290 3507
Fragments arranged by size:
3507 1290 556 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2245 4277 4451 65
Size: 1183 997 2032 174 1396
Fragments arranged by size:
2032 1396 1183 997 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3066 4426 4613 1700
Size: 110 1256 1360 187 2869
Fragments arranged by size:
2869 1360 1256 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5782
BssHII G'CGCG_C
Cuts at: 1925 2508 1925
Size: 583 5199
BssSI C'ACGA_G
Cuts at: 2354 3842 4854 2354
Size: 1488 1012 3282
Fragments arranged by size:
3282 1488 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5782
BstBI TT'CG_AA
Cuts at: 2614 4586 2614
Size: 1972 3810
BstEII G'GTnAC_C
Cuts at: 1184 2074 1184
Size: 890 4892
BstYI r'GATC_y
Cuts at: 1972 2931 3112 3479 3864 3881 5333 5419 5431
Size: 959 181 367 385 17 1452 86 12
Cuts at: 5431 5777 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 959 385 367 346 181 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2056 2056
Size: 5782
BtgI C'CryG_G
Cuts at: 513 1065 2004 2139 2325 2496 513
Size: 552 939 135 186 171 3799
Fragments arranged by size:
3799 939 552 186 171 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2885 487
Size: 683 56 1659 3384
Fragments arranged by size:
3384 1659 683 56
BtsI GCAGTG_nn'
Cuts at: 2430 2628 2667 2820 3298 4092 4112 2430
Size: 198 39 153 478 794 20 4100
Fragments arranged by size:
4100 794 478 198 153 39 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5747
DraI TTT'AAA
Cuts at: 3086 3086
Size: 5782
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4789 809
Size: 507 3473 1802
Fragments arranged by size:
3473 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 3229 4118
Size: 54 1425 50 275 18 312 1087 889
Cuts at: 4118 5747 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1087 889 312 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3229 1537
Size: 275 1417 4090
Fragments arranged by size:
4090 1417 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 2720 3712 1353
Size: 737 630 992 3423
Fragments arranged by size:
3423 992 737 630
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4369 4743 4889 387
Size: 1064 138 2780 374 146 1280
Fragments arranged by size:
2780 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2024 2149 2270 2395 2468 2570 3845
Size: 393 392 125 121 125 73 102 1275
Cuts at: 3845 4442 5229 5497 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1275 787 597 393 392 268 125
125 121 102 73
EcoO109I rG'GnC_Cy
Cuts at: 2017 2367 2662 2663 2017
Size: 350 295 1 5136
Fragments arranged by size:
5136 350 295 1
EcoRI G'AATT_C
Cuts at: 3091 3469 3091
Size: 378 5404
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2977 2977
Size: 5782
FspI TGC'GCA
Cuts at: 4288 4288
Size: 5782
HaeII r_GCGC'y
Cuts at: 1678 2644 4929 1678
Size: 966 2285 2531
Fragments arranged by size:
2531 2285 966
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4423 4455 4497 4529 1373
Size: 32 135 32 2851 32 42 32 2626
Fragments arranged by size:
2851 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3126 3372 669
Size: 988 281 1188 246 3079
Fragments arranged by size:
3079 1188 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3040 748
Size: 2292 3490
HpaI GTT'AAC
Cuts at: 3372 3372
Size: 5782
KpnI G_GTAC'C
Cuts at: 3107 3107
Size: 5782
MfeI C'AATT_G
Cuts at: 3381 3381
Size: 5782
MluI A'CGCG_T
Cuts at: 5658 5658
Size: 5782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4896 5080 1305
Size: 3591 184 2007
Fragments arranged by size:
3591 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2144 10
Size: 54 1425 343 312 3648
Fragments arranged by size:
3648 1425 343 312 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2396 3740 4099 4258 518
Size: 648 1230 1344 359 159 2042
Fragments arranged by size:
2042 1344 1230 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2131 2283 3198 3875 5023 5268 1606
Size: 525 152 915 677 1148 245 2120
Fragments arranged by size:
2120 1148 915 677 525 245 152
NcoI C'CATG_G
Cuts at: 513 1065 2004 2139 2325 2496 513
Size: 552 939 135 186 171 3799
Fragments arranged by size:
3799 939 552 186 171 135
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5782
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5782
NotI GC'GGCC_GC
Cuts at: 3229 3229
Size: 5782
NspI r_CATG'y
Cuts at: 1132 1229 2585 2903 3140 4685 1132
Size: 97 1356 318 237 1545 2229
Fragments arranged by size:
2229 1545 1356 318 237 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5782
PciI A'CATG_T
Cuts at: 4681 4681
Size: 5782
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2047 2172 1108
Size: 939 125 4718
Fragments arranged by size:
4718 939 125
PfoI T'CCnGG_A
Cuts at: 2247 2247
Size: 5782
PmeI GTTT'AAAC
Cuts at: 3086 3086
Size: 5782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 5364 5396 3851
Size: 32 1481 32 4237
Fragments arranged by size:
4237 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2017 2367 2017
Size: 350 5432
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5782
PsiI TTA'TAA
Cuts at: 3352 3352
Size: 5782
PspOMI G'GGCC_C
Cuts at: 2662 2662
Size: 5782
PstI C_TGCA'G
Cuts at: 830 2124 2574 2623 2815 3134 830
Size: 1294 450 49 192 319 3478
Fragments arranged by size:
3478 1294 450 319 192 49
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5782
SalI G'TCGA_C
Cuts at: 1936 3124 1936
Size: 1188 4594
SapI GCTCTTCn'nnn_
Cuts at: 2720 2720
Size: 5782
SbfI CC_TGCA'GG
Cuts at: 3134 3134
Size: 5782
ScaI AGT'ACT
Cuts at: 4030 5742 4030
Size: 1712 4070
SexAI A'CCwGG_T
Cuts at: 2291 2291
Size: 5782
SfcI C'TryA_G
Cuts at: 826 1045 2120 2570 2619 2811 3130 4265 4946
Size: 219 1075 450 49 192 319 1135 681
Cuts at: 4946 5137 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1075 681 450 319 219 192
191 49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5782
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5782
SmaI CCC'GGG
Cuts at: 1872 3109 1872
Size: 1237 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3886 4787 5049 820
Size: 197 926 1943 901 262 1553
Fragments arranged by size:
1943 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3573 493
Size: 3080 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5782
SphI G_CATG'C
Cuts at: 1132 3140 1132
Size: 2008 3774
SspI AAT'ATT
Cuts at: 5 52 3706 5
Size: 47 3654 2081
Fragments arranged by size:
3654 2081 47
StuI AGG'CCT
Cuts at: 1517 2453 1517
Size: 936 4846
StyI C'CwwG_G
Cuts at: 513 1065 2004 2139 2325 2496 2952 3221 513
Size: 552 939 135 186 171 456 269 3074
Fragments arranged by size:
3074 939 552 456 269 186 171 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2509 2591 3809 3826 3979 4164 4609
Size: 1255 929 82 1218 17 153 185 445
Cuts at: 4609 325
Size: 1498
Fragments arranged by size:
1498 1255 1218 929 445 185 153 82
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2700 4028 5740
Size: 274 80 33 51 1443 723 1328 1712
Cuts at: 5740 96
Size: 138
Fragments arranged by size:
1712 1443 1328 723 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5742 5774 5742
Size: 32 5750
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5782
XbaI T'CTAG_A
Cuts at: 3118 3118
Size: 5782
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5782
XmaI C'CCGG_G
Cuts at: 1870 3107 1870
Size: 1237 4545
XmnI GAAnn'nnTTC
Cuts at: 2453 3911 2453
Size: 1458 4324
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3590 276
Size: 53 83 186 936 2056 2468
Fragments arranged by size:
2468 2056 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII
SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI