Restriction map for
pFN21AE0101
(Circular) (Six-base) MAPSORT of: pFN21AE0101.seq Check: 5297 from: 1 to: 5251
pFN21AE0101 5251 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3061 278
Size: 53 83 186 936 1525 2468
Fragments arranged by size:
2468 1525 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2594 1937
Size: 657 4594
Acc65I G'GTAC_C
Cuts at: 2572 2572
Size: 5251
AclI AA'CG_TT
Cuts at: 1563 3378 3751 1563
Size: 1815 373 3063
Fragments arranged by size:
3063 1815 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3314 4698 4966 1632
Size: 1682 1384 268 1917
Fragments arranged by size:
1917 1682 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5054
AflIII A'CryG_T
Cuts at: 1203 4150 5127 1203
Size: 2947 977 1327
Fragments arranged by size:
2947 1327 977
AgeI A'CCGG_T
Cuts at: 2043 4082 2043
Size: 2039 3212
AhdI GACnn_n'nnGTC
Cuts at: 3980 3980
Size: 5251
AleI CACnn'nnGTG
Cuts at: 1166 2044 2492 1166
Size: 878 448 3925
Fragments arranged by size:
3925 878 448
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2072 1184
Size: 888 4363
AlwNI CAG_nnn'CTG
Cuts at: 2405 2994 4566 2405
Size: 589 1572 3090
Fragments arranged by size:
3090 1572 589
ApaLI G'TGCA_C
Cuts at: 3308 4464 3308
Size: 1156 4095
ApoI r'AATT_y
Cuts at: 1493 2066 2094 2384 2560 2786 2938 5047 1493
Size: 573 28 290 176 226 152 2109 1697
Fragments arranged by size:
2109 1697 573 290 226 176 152 28
AseI AT'TA_AT
Cuts at: 160 1032 2394 3805 160
Size: 872 1362 1411 1606
Fragments arranged by size:
1606 1411 1362 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5251
AvaI C'yCGr_G
Cuts at: 1870 1943 2341 2576 1870
Size: 73 398 235 4545
Fragments arranged by size:
4545 398 235 73
BamHI G'GATC_C
Cuts at: 2581 2948 2581
Size: 367 4884
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2083 2572 4027 618
Size: 325 217 627 296 489 1455 1842
Fragments arranged by size:
1842 1455 627 489 325 296 217
BanII G_rGCy'C
Cuts at: 1391 2570 1391
Size: 1179 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4280
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3442 3476 1903
Size: 34 1505 34 3678
Fragments arranged by size:
3678 1505 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3143 4353 3143
Size: 1210 4041
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5251
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2414 3862 136
Size: 107 122 71 631 605 742 1448 1525
Fragments arranged by size:
1525 1448 742 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2026 5246 2026
Size: 3220 2031
BlpI GC'TnA_GC
Cuts at: 2299 2668 2299
Size: 369 4882
Bme1580I G_kGCm'C
Cuts at: 1790 3312 4468 1790
Size: 1522 1156 2573
Fragments arranged by size:
2573 1522 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5251
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2421 2982 3940
Size: 416 53 83 399 24 1004 561 958
Cuts at: 3940 442
Size: 1753
Fragments arranged by size:
1753 1004 958 561 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3911 1239
Size: 2672 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2135 3376 4241 4539 2135
Size: 1241 865 298 2847
Fragments arranged by size:
2847 1241 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3914 882
Size: 617 2415 2219
Fragments arranged by size:
2415 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3042 493
Size: 711 1838 2702
Fragments arranged by size:
2702 1838 711
BsaBI GATnn'nnATC
Cuts at: 2199 2465 5245 2199
Size: 266 2780 2205
Fragments arranged by size:
2780 2205 266
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3058 3440 4075 275
Size: 53 83 186 936 1525 382 635 1451
Fragments arranged by size:
1525 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2043 3684 4082 4356 4503 4914 2043
Size: 1641 398 274 147 411 2380
Fragments arranged by size:
2380 1641 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2502 2532 1178
Size: 30 165 30 1099 30 3897
Fragments arranged by size:
3897 1099 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2199 1186
Size: 1013 4238
BseYI C'CCAG_C
Cuts at: 4454 4950 4454
Size: 496 4755
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2015 2095 1163
Size: 573 279 80 4319
Fragments arranged by size:
4319 573 279 80
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2701 3462 4490 1540
Size: 275 186 700 761 1028 2301
Fragments arranged by size:
2301 1028 761 700 275 186
BsiHKAI G_wGCw'C
Cuts at: 3312 3397 4468 3312
Size: 85 1071 4095
Fragments arranged by size:
4095 1071 85
BsmI GAATG_Cn'
Cuts at: 2762 2855 2762
Size: 93 5158
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2570 3312 3397 4468 1391
Size: 399 780 742 85 1071 2174
Fragments arranged by size:
2174 1071 780 742 399 85
BspEI T'CCGG_A
Cuts at: 4914 4914
Size: 5251
BspHI T'CATG_A
Cuts at: 3140 4870 3140
Size: 1730 3521
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2606 844
Size: 1762 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3138 1394
Size: 429 1315 3507
Fragments arranged by size:
3507 1315 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3746 3920 65
Size: 1183 2498 174 1396
Fragments arranged by size:
2498 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2043 3895 4082 1700
Size: 110 233 1852 187 2869
Fragments arranged by size:
2869 1852 233 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5251
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5251
BssSI C'ACGA_G
Cuts at: 3311 4323 3311
Size: 1012 4239
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2423 794
Size: 1629 3622
BstBI TT'CG_AA
Cuts at: 4055 4055
Size: 5251
BstEII G'GTnAC_C
Cuts at: 1184 2485 1184
Size: 1301 3950
BstYI r'GATC_y
Cuts at: 1972 2026 2581 2948 3333 3350 4802 4888 4900
Size: 54 555 367 385 17 1452 86 12
Cuts at: 4900 5246 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 555 385 367 346 86 54
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2260 2279 513
Size: 552 1195 19 3485
Fragments arranged by size:
3485 1195 552 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4512
Fragments arranged by size:
4512 683 56
BtsI GCAGTG_nn'
Cuts at: 2767 3561 3581 2767
Size: 794 20 4437
Fragments arranged by size:
4437 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5251
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5216
DraI TTT'AAA
Cuts at: 2555 2555
Size: 5251
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4258 809
Size: 507 2942 1802
Fragments arranged by size:
2942 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2292 2698 3587
Size: 54 1425 50 275 18 462 406 889
Cuts at: 3587 5216 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 462 406 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2698 1537
Size: 275 886 4090
Fragments arranged by size:
4090 886 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3181 1353
Size: 1828 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3838 4212 4358 387
Size: 1064 138 2249 374 146 1280
Fragments arranged by size:
2249 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3314 3911 4698 4966 1239
Size: 393 1682 597 787 268 1524
Fragments arranged by size:
1682 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2560 2938 2560
Size: 378 4873
EcoRV GAT'ATC
Cuts at: 2467 2467
Size: 5251
FspI TGC'GCA
Cuts at: 3757 3757
Size: 5251
HaeII r_GCGC'y
Cuts at: 1678 4398 1678
Size: 2720 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3892 3924 3966 3998 1373
Size: 32 135 32 2320 32 42 32 2626
Fragments arranged by size:
2626 2320 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2595 2841 669
Size: 988 281 657 246 3079
Fragments arranged by size:
3079 988 657 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5251
HpaI GTT'AAC
Cuts at: 2841 2841
Size: 5251
KpnI G_GTAC'C
Cuts at: 2576 2576
Size: 5251
MfeI C'AATT_G
Cuts at: 2850 2850
Size: 5251
MluI A'CGCG_T
Cuts at: 5127 5127
Size: 5251
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2215 4365 4549 1305
Size: 910 2150 184 2007
Fragments arranged by size:
2150 2007 910 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2294 10
Size: 54 1425 343 462 2967
Fragments arranged by size:
2967 1425 462 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2044 2492 3209 3568 3727 518
Size: 648 843 35 448 717 359 159 2042
Fragments arranged by size:
2042 843 717 648 448 359 159 35
MspA1I CmG'CkG
Cuts at: 1606 2667 3344 4492 4737 1606
Size: 1061 677 1148 245 2120
Fragments arranged by size:
2120 1148 1061 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2260 2279 513
Size: 552 1195 19 3485
Fragments arranged by size:
3485 1195 552 19
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5251
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5251
NotI GC'GGCC_GC
Cuts at: 2698 2698
Size: 5251
NspI r_CATG'y
Cuts at: 1132 1229 2609 4154 1132
Size: 97 1380 1545 2229
Fragments arranged by size:
2229 1545 1380 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5251
PciI A'CATG_T
Cuts at: 4150 4150
Size: 5251
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5251
PmeI GTTT'AAAC
Cuts at: 2555 2555
Size: 5251
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3320 3352 4833 4865 3320
Size: 32 1481 32 3706
Fragments arranged by size:
3706 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5251
PsiI TTA'TAA
Cuts at: 2821 2821
Size: 5251
PstI C_TGCA'G
Cuts at: 830 2603 830
Size: 1773 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5251
SalI G'TCGA_C
Cuts at: 1936 2593 1936
Size: 657 4594
SbfI CC_TGCA'GG
Cuts at: 2603 2603
Size: 5251
ScaI AGT'ACT
Cuts at: 3499 5211 3499
Size: 1712 3539
SfcI C'TryA_G
Cuts at: 826 1045 2478 2599 3734 4415 4606 826
Size: 219 1433 121 1135 681 191 1471
Fragments arranged by size:
1471 1433 1135 681 219 191 121
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5251
SgrAI Cr'CCGG_yG
Cuts at: 1700 2043 1700
Size: 343 4908
SmaI CCC'GGG
Cuts at: 1872 2578 1872
Size: 706 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2150 3355 4256 4518 820
Size: 197 926 207 1205 901 262 1553
Fragments arranged by size:
1553 1205 926 901 262 207 197
SnaBI TAC'GTA
Cuts at: 493 3042 493
Size: 2549 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5251
SphI G_CATG'C
Cuts at: 1132 2609 1132
Size: 1477 3774
SspI AAT'ATT
Cuts at: 5 52 3175 5
Size: 47 3123 2081
Fragments arranged by size:
3123 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5251
StyI C'CwwG_G
Cuts at: 513 1065 2260 2279 2427 2690 513
Size: 552 1195 19 148 263 3074
Fragments arranged by size:
3074 1195 552 263 148 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3278 3295 3448 3633 4078 325
Size: 1255 1698 17 153 185 445 1498
Fragments arranged by size:
1698 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3497 5209 96
Size: 274 80 33 51 1443 1520 1712 138
Fragments arranged by size:
1712 1520 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2502 2534 5211 5243 2502
Size: 32 2677 32 2510
Fragments arranged by size:
2677 2510 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5251
XbaI T'CTAG_A
Cuts at: 2587 2587
Size: 5251
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5251
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5251
XmaI C'CCGG_G
Cuts at: 1870 2576 1870
Size: 706 4545
XmnI GAAnn'nnTTC
Cuts at: 3380 3380
Size: 5251
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3059 276
Size: 53 83 186 936 1525 2468
Fragments arranged by size:
2468 1525 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI