Restriction map for
pFN21AE0099
(Circular) (Six-base) MAPSORT of: pFN21AE0099.seq Check: 40 from: 1 to: 5506
pFN21AE0099 5506 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3316 278
Size: 53 83 186 936 1780 2468
Fragments arranged by size:
2468 1780 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2849 1937
Size: 912 4594
Acc65I G'GTAC_C
Cuts at: 2827 2827
Size: 5506
AclI AA'CG_TT
Cuts at: 1563 3633 4006 1563
Size: 2070 373 3063
Fragments arranged by size:
3063 2070 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2230 3569 4953 5221 1632
Size: 598 1339 1384 268 1917
Fragments arranged by size:
1917 1384 1339 598 268
AfeI AGC'GCT
Cuts at: 2594 2770 2594
Size: 176 5330
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5309
AflIII A'CryG_T
Cuts at: 1203 4405 5382 1203
Size: 3202 977 1327
Fragments arranged by size:
3202 1327 977
AgeI A'CCGG_T
Cuts at: 4337 4337
Size: 5506
AhdI GACnn_n'nnGTC
Cuts at: 2164 4235 2164
Size: 2071 3435
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5506
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5506
AlwNI CAG_nnn'CTG
Cuts at: 2476 3249 4821 2476
Size: 773 1572 3161
Fragments arranged by size:
3161 1572 773
ApaLI G'TGCA_C
Cuts at: 3563 4719 3563
Size: 1156 4350
ApoI r'AATT_y
Cuts at: 1493 2815 3041 3193 5302 1493
Size: 1322 226 152 2109 1697
Fragments arranged by size:
2109 1697 1322 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4060 160
Size: 872 3028 1606
Fragments arranged by size:
3028 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5506
AvaI C'yCGr_G
Cuts at: 1870 1943 2708 2774 2831 1870
Size: 73 765 66 57 4545
Fragments arranged by size:
4545 765 73 66 57
BamHI G'GATC_C
Cuts at: 2836 3203 2836
Size: 367 5139
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2633 2827 4282 618
Size: 325 217 627 846 194 1455 1842
Fragments arranged by size:
1842 1455 846 627 325 217 194
BanII G_rGCy'C
Cuts at: 1391 2096 2418 2454 2504 2825 1391
Size: 705 322 36 50 321 4072
Fragments arranged by size:
4072 705 322 321 50 36
BbeI G_GCGC'C
Cuts at: 2637 2637
Size: 5506
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4535
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3697 3731 1903
Size: 34 1760 34 3678
Fragments arranged by size:
3678 1760 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3398 4608 3398
Size: 1210 4296
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5506
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2508 2642 4117
Size: 107 122 71 631 605 836 134 1475
Cuts at: 4117 136
Size: 1525
Fragments arranged by size:
1525 1475 836 631 605 134 122 107
71
BglII A'GATC_T
Cuts at: 5501 5501
Size: 5506
BlpI GC'TnA_GC
Cuts at: 2498 2684 2923 2498
Size: 186 239 5081
Fragments arranged by size:
5081 239 186
Bme1580I G_kGCm'C
Cuts at: 1790 3567 4723 1790
Size: 1777 1156 2573
Fragments arranged by size:
2573 1777 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5506
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2164 2233 2516
Size: 416 53 83 399 24 747 69 283
Cuts at: 2516 3237 4195 442
Size: 721 958 1753
Fragments arranged by size:
1753 958 747 721 416 399 283 83
69 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5506
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2073 2073
Size: 5506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2366 2762 4166 1239
Size: 1127 396 1404 2579
Fragments arranged by size:
2579 1404 1127 396
Bpu10I CC'TnA_GC
Cuts at: 2765 2765
Size: 5506
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2603 2630 3631 4496 4794 2603
Size: 27 1001 865 298 3315
Fragments arranged by size:
3315 1001 865 298 27
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4169 882
Size: 617 2670 2219
Fragments arranged by size:
2670 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2131 3297 493
Size: 711 927 1166 2702
Fragments arranged by size:
2702 1166 927 711
BsaBI GATnn'nnATC
Cuts at: 5500 5500
Size: 5506
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2634 3313 3695 4330
Size: 53 83 186 936 1101 679 382 635
Cuts at: 4330 275
Size: 1451
Fragments arranged by size:
1451 1101 936 679 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3939 4337 4611 4758 5169 3939
Size: 398 274 147 411 4276
Fragments arranged by size:
4276 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5281
Fragments arranged by size:
5281 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2363 2420 2528 2792 1186
Size: 1177 57 108 264 3900
Fragments arranged by size:
3900 1177 264 108 57
BseYI C'CCAG_C
Cuts at: 4709 5205 4709
Size: 496 5010
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2387 2462 1163
Size: 573 651 75 4207
Fragments arranged by size:
4207 651 573 75
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2527 2956 3717 4745 1540
Size: 275 186 526 429 761 1028 2301
Fragments arranged by size:
2301 1028 761 526 429 275 186
BsiHKAI G_wGCw'C
Cuts at: 2037 2142 2418 2454 2681 3567 3652 4723 2037
Size: 105 276 36 227 886 85 1071 2820
Fragments arranged by size:
2820 1071 886 276 227 105 85 36
BsmI GAATG_Cn'
Cuts at: 3017 3110 3017
Size: 93 5413
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2096 2142 2418 2454 2504 2681
Size: 399 247 59 46 276 36 50 177
Cuts at: 2681 2825 3567 3652 4723 1391
Size: 144 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 276 247 177 144
85 59 50 46 36
BspEI T'CCGG_A
Cuts at: 5169 5169
Size: 5506
BspHI T'CATG_A
Cuts at: 3395 5125 3395
Size: 1730 3776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2861 844
Size: 2017 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3393 1394
Size: 429 1570 3507
Fragments arranged by size:
3507 1570 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4001 4175 65
Size: 1183 2753 174 1396
Fragments arranged by size:
2753 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4150 4337 1700
Size: 110 2340 187 2869
Fragments arranged by size:
2869 2340 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5506
BssHII G'CGCG_C
Cuts at: 1925 2443 2463 2465 2529 2531 2533 2603 1925
Size: 518 20 2 64 2 2 70 4828
Fragments arranged by size:
4828 518 70 64 20 2 2 2
BssSI C'ACGA_G
Cuts at: 2480 3566 4578 2480
Size: 1086 1012 3408
Fragments arranged by size:
3408 1086 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2485 794
Size: 1691 3815
BstBI TT'CG_AA
Cuts at: 4310 4310
Size: 5506
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5506
BstYI r'GATC_y
Cuts at: 1972 2341 2836 3203 3588 3605 5057 5143 5155
Size: 369 495 367 385 17 1452 86 12
Cuts at: 5155 5501 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 495 385 369 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2321 2321
Size: 5506
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4954
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4767
Fragments arranged by size:
4767 683 56
BtsI GCAGTG_nn'
Cuts at: 3022 3816 3836 3022
Size: 794 20 4692
Fragments arranged by size:
4692 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5506
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5471
DraI TTT'AAA
Cuts at: 2810 2810
Size: 5506
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4513 809
Size: 507 3197 1802
Fragments arranged by size:
3197 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2118 2598
Size: 54 1425 50 275 18 198 90 480
Cuts at: 2598 2643 2953 3842 5471 8
Size: 45 310 889 1629 43
Fragments arranged by size:
1629 1425 889 480 310 275 198 90
54 50 45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2953 1537
Size: 275 1141 4090
Fragments arranged by size:
4090 1141 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2042 3436 1353
Size: 689 1394 3423
Fragments arranged by size:
3423 1394 689
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2173 2708 4093 4467 4613 387
Size: 1064 138 584 535 1385 374 146 1280
Fragments arranged by size:
1385 1280 1064 584 535 374 146 138
EcoICRI GAG'CTC
Cuts at: 2416 2452 2416
Size: 36 5470
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2230 2366 2762 3569 4166 4953 5221
Size: 393 598 136 396 807 597 787 268
Cuts at: 5221 1239
Size: 1524
Fragments arranged by size:
1524 807 787 598 597 396 393 268
136
EcoNI CCTnn'n_nnAGG
Cuts at: 2303 2324 2303
Size: 21 5485
EcoO109I rG'GnC_Cy
Cuts at: 2113 2317 2713 2113
Size: 204 396 4906
Fragments arranged by size:
4906 396 204
EcoRI G'AATT_C
Cuts at: 2815 3193 2815
Size: 378 5128
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2776 2776
Size: 5506
FspI TGC'GCA
Cuts at: 2555 4012 2555
Size: 1457 4049
HaeII r_GCGC'y
Cuts at: 1678 2596 2637 2772 4653 1678
Size: 918 41 135 1881 2531
Fragments arranged by size:
2531 1881 918 135 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2150 2182 2326 2358 4147
Size: 32 135 32 578 32 144 32 1789
Cuts at: 4147 4179 4221 4253 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1789 578 144 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2850 3096 669
Size: 988 281 912 246 3079
Fragments arranged by size:
3079 988 912 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5506
HpaI GTT'AAC
Cuts at: 3096 3096
Size: 5506
KasI G'GCGC_C
Cuts at: 2633 2633
Size: 5506
KpnI G_GTAC'C
Cuts at: 2831 2831
Size: 5506
MfeI C'AATT_G
Cuts at: 3105 3105
Size: 5506
MluI A'CGCG_T
Cuts at: 5382 5382
Size: 5506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4620 4804 1305
Size: 3315 184 2007
Fragments arranged by size:
3315 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2120 10
Size: 54 1425 343 288 3396
Fragments arranged by size:
3396 1425 343 288 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3464 3823 3982 518
Size: 648 2298 359 159 2042
Fragments arranged by size:
2298 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2151 2440 2922 3599 4747 4992 1606
Size: 545 289 482 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 545 482 289 245
NarI GG'CG_CC
Cuts at: 2634 2634
Size: 5506
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4954
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5506
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5506
NotI GC'GGCC_GC
Cuts at: 2953 2953
Size: 5506
NspI r_CATG'y
Cuts at: 1132 1229 2864 4409 1132
Size: 97 1635 1545 2229
Fragments arranged by size:
2229 1635 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2097 1348
Size: 749 4757
PciI A'CATG_T
Cuts at: 4405 4405
Size: 5506
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2431 2596 1108
Size: 1323 165 4018
Fragments arranged by size:
4018 1323 165
PmeI GTTT'AAAC
Cuts at: 2810 2810
Size: 5506
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3575 3607 5088 5120 3575
Size: 32 1481 32 3961
Fragments arranged by size:
3961 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2113 2713 2113
Size: 600 4906
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5506
PsiI TTA'TAA
Cuts at: 3076 3076
Size: 5506
PspXI vC'TCGA_Gb
Cuts at: 2708 2708
Size: 5506
PstI C_TGCA'G
Cuts at: 830 2858 830
Size: 2028 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5506
SacI G_AGCT'C
Cuts at: 2418 2454 2418
Size: 36 5470
SalI G'TCGA_C
Cuts at: 1936 2848 1936
Size: 912 4594
SapI GCTCTTCn'nnn_
Cuts at: 2042 2042
Size: 5506
SbfI CC_TGCA'GG
Cuts at: 2858 2858
Size: 5506
ScaI AGT'ACT
Cuts at: 3754 5466 3754
Size: 1712 3794
SfcI C'TryA_G
Cuts at: 826 1045 2572 2854 3989 4670 4861 826
Size: 219 1527 282 1135 681 191 1471
Fragments arranged by size:
1527 1471 1135 681 282 219 191
SfoI GGC'GCC
Cuts at: 2635 2635
Size: 5506
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5506
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5506
SmaI CCC'GGG
Cuts at: 1872 2833 1872
Size: 961 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2609 2618 2708 2774 3610 4511
Size: 197 926 666 9 90 66 836 901
Cuts at: 4511 4773 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 836 666 262 197 90
66 9
SnaBI TAC'GTA
Cuts at: 493 3297 493
Size: 2804 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5506
SphI G_CATG'C
Cuts at: 1132 2864 1132
Size: 1732 3774
SspI AAT'ATT
Cuts at: 5 52 3430 5
Size: 47 3378 2081
Fragments arranged by size:
3378 2081 47
StuI AGG'CCT
Cuts at: 1517 2727 1517
Size: 1210 4296
StyI C'CwwG_G
Cuts at: 513 1065 2025 2187 2361 2658 2722 2945 513
Size: 552 960 162 174 297 64 223 3074
Fragments arranged by size:
3074 960 552 297 223 174 162 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2816 3533 3550 3703 3888 4333 325
Size: 1255 1236 717 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1236 717 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2781 3752 5464
Size: 274 80 33 51 1443 804 971 1712
Cuts at: 5464 96
Size: 138
Fragments arranged by size:
1712 1443 971 804 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5466 5498 5466
Size: 32 5474
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5506
XbaI T'CTAG_A
Cuts at: 2842 2842
Size: 5506
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2128 2128
Size: 5506
XhoI C'TCGA_G
Cuts at: 1943 2708 2774 1943
Size: 765 66 4675
Fragments arranged by size:
4675 765 66
XmaI C'CCGG_G
Cuts at: 1870 2831 1870
Size: 961 4545
XmnI GAAnn'nnTTC
Cuts at: 3635 3635
Size: 5506
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3314 276
Size: 53 83 186 936 1780 2468
Fragments arranged by size:
2468 1780 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI
SwaI