Restriction map for
pFN21AE0096
(Circular) (Six-base) MAPSORT of: pFN21AE0096.seq Check: 528 from: 1 to: 10210
pFN21AE0096 10210 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3919 5563 3919
Size: 1644 8566
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8020 278
Size: 53 83 186 936 6484 2468
Fragments arranged by size:
6484 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5914 6295 6518 7553 1937
Size: 3977 381 223 1035 4594
Fragments arranged by size:
4594 3977 1035 381 223
Acc65I G'GTAC_C
Cuts at: 2341 4339 7531 2341
Size: 1998 3192 5020
Fragments arranged by size:
5020 3192 1998
AclI AA'CG_TT
Cuts at: 1563 8337 8710 1563
Size: 6774 373 3063
Fragments arranged by size:
6774 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2849 4341 4584 8273 9657 9925 1632
Size: 1217 1492 243 3689 1384 268 1917
Fragments arranged by size:
3689 1917 1492 1384 1217 268 243
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10013
AflIII A'CryG_T
Cuts at: 1203 3336 5773 9109 10086 1203
Size: 2133 2437 3336 977 1327
Fragments arranged by size:
3336 2437 2133 1327 977
AgeI A'CCGG_T
Cuts at: 9041 9041
Size: 10210
AhdI GACnn_n'nnGTC
Cuts at: 6514 8939 6514
Size: 2425 7785
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10210
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3546 4110 6237 1184
Size: 2362 564 2127 5157
Fragments arranged by size:
5157 2362 2127 564
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3712 3744 3712
Size: 32 10178
AlwNI CAG_nnn'CTG
Cuts at: 4106 5194 6968 7953 9525 4106
Size: 1088 1774 985 1572 4791
Fragments arranged by size:
4791 1774 1572 1088 985
ApaLI G'TGCA_C
Cuts at: 8267 9423 8267
Size: 1156 9054
ApoI r'AATT_y
Cuts at: 1493 3132 4053 4165 4276 4609 5937 6246 6373
Size: 1639 921 112 111 333 1328 309 127
Cuts at: 6373 6591 7519 7745 7897 10006 1493
Size: 218 928 226 152 2109 1697
Fragments arranged by size:
2109 1697 1639 1328 928 921 333 309
226 218 152 127 112 111
AseI AT'TA_AT
Cuts at: 160 1032 8764 160
Size: 872 7732 1606
Fragments arranged by size:
7732 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10210
AvaI C'yCGr_G
Cuts at: 1870 1943 3260 3627 7535 1870
Size: 73 1317 367 3908 4545
Fragments arranged by size:
4545 3908 1317 367 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4323 4356 4323
Size: 33 10177
BamHI G'GATC_C
Cuts at: 2648 6854 7540 7907 2648
Size: 4206 686 367 4951
Fragments arranged by size:
4951 4206 686 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2341 4339 7531 8986
Size: 325 217 627 239 315 1998 3192 1455
Cuts at: 8986 618
Size: 1842
Fragments arranged by size:
3192 1998 1842 1455 627 325 315 239
217
BanII G_rGCy'C
Cuts at: 1391 5281 7529 1391
Size: 3890 2248 4072
Fragments arranged by size:
4072 3890 2248
BbeI G_GCGC'C
Cuts at: 2030 2030
Size: 10210
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2693 2933 7134 928
Size: 971 794 240 4201 4004
Fragments arranged by size:
4201 4004 971 794 240
BbvCI CC'TCA_GC
Cuts at: 6387 6387
Size: 10210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8401 8435 1903
Size: 34 6464 34 3678
Fragments arranged by size:
6464 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5842 6493 6559 8102 9312 5842
Size: 651 66 1543 1210 6740
Fragments arranged by size:
6740 1543 1210 651 66
BclI T'GATC_A
Cuts at: 1548 6232 1548
Size: 4684 5526
BfrBI ATG'CAT
Cuts at: 5869 5869
Size: 10210
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8821 136
Size: 107 122 71 631 605 7149 1525
Fragments arranged by size:
7149 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 10205 10205
Size: 10210
BlpI GC'TnA_GC
Cuts at: 3024 5355 7627 3024
Size: 2331 2272 5607
Fragments arranged by size:
5607 2331 2272
Bme1580I G_kGCm'C
Cuts at: 1790 5436 7172 8271 9427 1790
Size: 3646 1736 1099 1156 2573
Fragments arranged by size:
3646 2573 1736 1156 1099
BmgBI CAC'GTC
Cuts at: 1318 2572 1318
Size: 1254 8956
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 7209 7941 8899
Size: 416 53 83 399 24 5792 732 958
Cuts at: 8899 442
Size: 1753
Fragments arranged by size:
5792 1753 958 732 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 7406 1056
Size: 6350 3860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3525 4083 5334 5580 6888 8870 1239
Size: 2286 558 1251 246 1308 1982 2579
Fragments arranged by size:
2579 2286 1982 1308 1251 558 246
Bpu10I CC'TnA_GC
Cuts at: 6387 6387
Size: 10210
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4654 5649 5893 8335 9200 9498 4654
Size: 995 244 2442 865 298 5366
Fragments arranged by size:
5366 2442 995 865 298 244
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2156 8873 882
Size: 617 657 6717 2219
Fragments arranged by size:
6717 2219 657 617
BsaAI yAC'GTr
Cuts at: 493 1204 8001 493
Size: 711 6797 2702
Fragments arranged by size:
6797 2702 711
BsaBI GATnn'nnATC
Cuts at: 6990 10204 6990
Size: 3214 6996
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 8017 8399 9034
Size: 53 83 186 936 494 5990 382 635
Cuts at: 9034 275
Size: 1451
Fragments arranged by size:
5990 1451 936 635 494 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2032 8643 9041 9315 9462 9873 2032
Size: 6611 398 274 147 411 2369
Fragments arranged by size:
6611 2369 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2205 2235 4176 4206 5689
Size: 30 165 30 802 30 1941 30 1483
Cuts at: 5689 5719 5795 5825 1178
Size: 30 76 30 5563
Fragments arranged by size:
5563 1941 1483 802 165 76 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2915 4224 5759 5838 7252 1186
Size: 1729 1309 1535 79 1414 4144
Fragments arranged by size:
4144 1729 1535 1414 1309 79
BseYI C'CCAG_C
Cuts at: 2830 3499 4936 5242 5482 9413 9909 2830
Size: 669 1437 306 240 3931 496 3131
Fragments arranged by size:
3931 3131 1437 669 496 306 240
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2946 5357 6833 7206 1163
Size: 573 1210 2411 1476 373 4167
Fragments arranged by size:
4167 2411 1476 1210 573 373
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7660 8421 9449 1540
Size: 275 186 5659 761 1028 2301
Fragments arranged by size:
5659 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5281 5963 7192 8271 8356 9427 5281
Size: 682 1229 1079 85 1071 6064
Fragments arranged by size:
6064 1229 1079 1071 682 85
BsmI GAATG_Cn'
Cuts at: 5146 6542 7721 7814 5146
Size: 1396 1179 93 7542
Fragments arranged by size:
7542 1396 1179 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 5281 5436 5963 7172 7192 7529 8271
Size: 399 3491 155 527 1209 20 337 742
Cuts at: 8271 8356 9427 1391
Size: 85 1071 2174
Fragments arranged by size:
3491 2174 1209 1071 742 527 399 337
155 85 20
BspEI T'CCGG_A
Cuts at: 2032 9873 2032
Size: 7841 2369
BspHI T'CATG_A
Cuts at: 2773 5779 8099 9829 2773
Size: 3006 2320 1730 3154
Fragments arranged by size:
3154 3006 2320 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3919 5068 5315 5563 7498 7565 844
Size: 3075 1149 247 248 1935 67 3489
Fragments arranged by size:
3489 3075 1935 1149 248 247 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2041 5616 8097 1394
Size: 429 218 3575 2481 3507
Fragments arranged by size:
3575 3507 2481 429 218
BsrDI GCAATG_nn'
Cuts at: 65 1248 2914 3775 4286 4689 5409 5589 5848
Size: 1183 1666 861 511 403 720 180 259
Cuts at: 5848 8705 8879 65
Size: 2857 174 1396
Fragments arranged by size:
2857 1666 1396 1183 861 720 511 403
259 180 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8854 9041 1700
Size: 110 7044 187 2869
Fragments arranged by size:
7044 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4015 6757 6805 96
Size: 3919 2742 48 3501
Fragments arranged by size:
3919 3501 2742 48
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10210
BssSI C'ACGA_G
Cuts at: 3675 8270 9282 3675
Size: 4595 1012 4603
Fragments arranged by size:
4603 4595 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2575 3392 5675 794
Size: 1781 817 2283 5329
Fragments arranged by size:
5329 2283 1781 817
BstBI TT'CG_AA
Cuts at: 5441 9014 5441
Size: 3573 6637
BstEII G'GTnAC_C
Cuts at: 1184 5518 1184
Size: 4334 5876
BstYI r'GATC_y
Cuts at: 1972 2140 2590 2648 4350 6685 6854 6871 7540
Size: 168 450 58 1702 2335 169 17 669
Cuts at: 7540 7907 8292 8309 9761 9847 9859 10205 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2335 1977 1702 1452 669 450 385 367
346 169 168 86 58 17 17 12
BstZ17I GTA'TAC
Cuts at: 6296 6296
Size: 10210
Bsu36I CC'TnA_GG
Cuts at: 5289 5413 5289
Size: 124 10086
BtgI C'CryG_G
Cuts at: 513 1065 2004 2451 513
Size: 552 939 447 8272
Fragments arranged by size:
8272 939 552 447
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9471
Fragments arranged by size:
9471 683 56
BtsI GCAGTG_nn'
Cuts at: 2880 7007 7726 8520 8540 2880
Size: 4127 719 794 20 4550
Fragments arranged by size:
4550 4127 794 719 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10210
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2502 2537 2647 2682 529
Size: 35 1938 35 110 35 8057
Fragments arranged by size:
8057 1938 110 35 35 35
DraI TTT'AAA
Cuts at: 2107 2271 3114 5886 7514 2107
Size: 164 843 2772 1628 4803
Fragments arranged by size:
4803 2772 1628 843 164
DraIII CAC_nnn'GTG
Cuts at: 2416 4336 5225 2416
Size: 1920 889 7401
Fragments arranged by size:
7401 1920 889
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9217 809
Size: 507 7901 1802
Fragments arranged by size:
7901 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5307 7657 8546
Size: 54 1425 50 275 18 3477 2350 889
Cuts at: 8546 10175 8
Size: 1629 43
Fragments arranged by size:
3477 2350 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7657 1537
Size: 275 5845 4090
Fragments arranged by size:
5845 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2220 3740 4869 8140 1353
Size: 867 1520 1129 3271 3423
Fragments arranged by size:
3423 3271 1520 1129 867
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2193 8797 9171 9317 387
Size: 1064 138 604 6604 374 146 1280
Fragments arranged by size:
6604 1280 1064 604 374 146 138
EcoICRI GAG'CTC
Cuts at: 5279 5279
Size: 10210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2849 3525 4083 4341 4584 5334 5580
Size: 393 1217 676 558 258 243 750 246
Cuts at: 5580 6888 8273 8870 9657 9925 1239
Size: 1308 1385 597 787 268 1524
Fragments arranged by size:
1524 1385 1308 1217 787 750 676 597
558 393 268 258 246 243
EcoNI CCTnn'n_nnAGG
Cuts at: 4017 4017
Size: 10210
EcoO109I rG'GnC_Cy
Cuts at: 4214 4214
Size: 10210
EcoRI G'AATT_C
Cuts at: 4053 4165 4276 6373 6591 7519 7897 4053
Size: 112 111 2097 218 928 378 6366
Fragments arranged by size:
6366 2097 928 378 218 112 111
EcoRV GAT'ATC
Cuts at: 6049 6174 6205 6049
Size: 125 31 10054
Fragments arranged by size:
10054 125 31
FspI TGC'GCA
Cuts at: 8716 8716
Size: 10210
HaeII r_GCGC'y
Cuts at: 1678 2030 9357 1678
Size: 352 7327 2531
Fragments arranged by size:
7327 2531 352
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3202 3234 3823 3855 5795
Size: 32 135 32 1630 32 589 32 1940
Cuts at: 5795 5827 6435 6458 6467 6490 6500 6532 8851
Size: 32 608 23 9 23 10 32 2319
Cuts at: 8851 8883 8925 8957 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2319 1940 1630 608 589 135 42
32 32 32 32 32 32 32 32
23 23 10 9
HincII GTy'rAC
Cuts at: 669 1657 1938 4460 7554 7800 669
Size: 988 281 2522 3094 246 3079
Fragments arranged by size:
3094 3079 2522 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5231 7422 748
Size: 4483 2191 3536
Fragments arranged by size:
4483 3536 2191
HpaI GTT'AAC
Cuts at: 7800 7800
Size: 10210
KasI G'GCGC_C
Cuts at: 2026 2026
Size: 10210
KpnI G_GTAC'C
Cuts at: 2345 4343 7535 2345
Size: 1998 3192 5020
Fragments arranged by size:
5020 3192 1998
MfeI C'AATT_G
Cuts at: 7809 7809
Size: 10210
MluI A'CGCG_T
Cuts at: 10086 10086
Size: 10210
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2400 4186 5614 7333 9324 9508 1305
Size: 1095 1786 1428 1719 1991 184 2007
Fragments arranged by size:
2007 1991 1786 1719 1428 1095 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5309 10
Size: 54 1425 343 3477 4911
Fragments arranged by size:
4911 3477 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3284 3335 3341 3786 3830 5778 6785
Size: 648 2118 51 6 445 44 1948 1007
Cuts at: 6785 7215 8168 8527 8686 518
Size: 430 953 359 159 2042
Fragments arranged by size:
2118 2042 1948 1007 953 648 445 430
359 159 51 44 6
MspA1I CmG'CkG
Cuts at: 1606 2857 3023 4517 4871 6803 7039 7319 7626
Size: 1251 166 1494 354 1932 236 280 307
Cuts at: 7626 8303 9451 9696 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1932 1494 1251 1148 677 354 307
280 245 236 166
NarI GG'CG_CC
Cuts at: 2027 2027
Size: 10210
NcoI C'CATG_G
Cuts at: 513 1065 2004 2451 513
Size: 552 939 447 8272
Fragments arranged by size:
8272 939 552 447
NdeI CA'TA_TG
Cuts at: 387 4404 6754 387
Size: 4017 2350 3843
Fragments arranged by size:
4017 3843 2350
NheI G'CTAG_C
Cuts at: 1052 7402 1052
Size: 6350 3860
NotI GC'GGCC_GC
Cuts at: 7657 7657
Size: 10210
NruI TCG'CGA
Cuts at: 2186 2186
Size: 10210
NsiI A_TGCA'T
Cuts at: 5871 5871
Size: 10210
NspI r_CATG'y
Cuts at: 1132 1229 3277 3340 3530 4437 5777 5873 6043
Size: 97 2048 63 190 907 1340 96 170
Cuts at: 6043 7568 9113 1132
Size: 1525 1545 2229
Fragments arranged by size:
2229 2048 1545 1525 1340 907 190 170
97 96 63
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10210
PciI A'CATG_T
Cuts at: 3336 5773 9109 3336
Size: 2437 3336 4437
Fragments arranged by size:
4437 3336 2437
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6851 1108
Size: 5743 4467
PfoI T'CCnGG_A
Cuts at: 2061 3541 2061
Size: 1480 8730
PmeI GTTT'AAAC
Cuts at: 7514 7514
Size: 10210
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8279 8311 9792 9824 8279
Size: 32 1481 32 8665
Fragments arranged by size:
8665 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4214 4214
Size: 10210
PshAI GACnn'nnGTC
Cuts at: 1613 5910 1613
Size: 4297 5913
PsiI TTA'TAA
Cuts at: 2478 3708 7780 2478
Size: 1230 4072 4908
Fragments arranged by size:
4908 4072 1230
PspXI vC'TCGA_Gb
Cuts at: 3260 3260
Size: 10210
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4008 4040 4008
Size: 32 10178
PstI C_TGCA'G
Cuts at: 830 3916 6653 7509 7562 830
Size: 3086 2737 856 53 3478
Fragments arranged by size:
3478 3086 2737 856 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10210
PvuII CAG'CTG
Cuts at: 3023 4517 4871 6803 7319 3023
Size: 1494 354 1932 516 5914
Fragments arranged by size:
5914 1932 1494 516 354
SacI G_AGCT'C
Cuts at: 5281 5281
Size: 10210
SalI G'TCGA_C
Cuts at: 1936 7552 1936
Size: 5616 4594
SapI GCTCTTCn'nnn_
Cuts at: 4869 4869
Size: 10210
SbfI CC_TGCA'GG
Cuts at: 3916 7562 3916
Size: 3646 6564
ScaI AGT'ACT
Cuts at: 8458 10170 8458
Size: 1712 8498
SexAI A'CCwGG_T
Cuts at: 5221 5514 5221
Size: 293 9917
SfcI C'TryA_G
Cuts at: 826 1045 3202 3459 3912 4152 4443 4623 4728
Size: 219 2157 257 453 240 291 180 105
Cuts at: 4728 6649 6696 7161 7505 7558 8693 9374 9565
Size: 1921 47 465 344 53 1135 681 191
Cuts at: 9565 826
Size: 1471
Fragments arranged by size:
2157 1921 1471 1135 681 465 453 344
291 257 240 219 191 180 105 53
47
SfoI GGC'GCC
Cuts at: 2028 2028
Size: 10210
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10210
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10210
SmaI CCC'GGG
Cuts at: 1872 7537 1872
Size: 5665 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3260 3627 4669 5664 5908 8314
Size: 197 926 1317 367 1042 995 244 2406
Cuts at: 8314 9215 9477 820
Size: 901 262 1553
Fragments arranged by size:
2406 1553 1317 1042 995 926 901 367
262 244 197
SnaBI TAC'GTA
Cuts at: 493 8001 493
Size: 7508 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10210
SphI G_CATG'C
Cuts at: 1132 7568 1132
Size: 6436 3774
SspI AAT'ATT
Cuts at: 5 52 3016 4585 5454 7329 7339 7349 8134
Size: 47 2964 1569 869 1875 10 10 785
Cuts at: 8134 5
Size: 2081
Fragments arranged by size:
2964 2081 1875 1569 869 785 47 10
10
StuI AGG'CCT
Cuts at: 1517 2997 1517
Size: 1480 8730
StyI C'CwwG_G
Cuts at: 513 1065 2004 2451 5497 7649 513
Size: 552 939 447 3046 2152 3074
Fragments arranged by size:
3074 3046 2152 939 552 447
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2114 3429 6263 6504 8237 8254 8407
Size: 1255 534 1315 2834 241 1733 17 153
Cuts at: 8407 8592 9037 325
Size: 185 445 1498
Fragments arranged by size:
2834 1733 1498 1315 1255 534 445 241
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3834 4015 4247
Size: 274 80 33 51 1443 1857 181 232
Cuts at: 4247 4417 4436 4603 5086 5110 5422 5644 5693
Size: 170 19 167 483 24 312 222 49
Cuts at: 5693 5898 6757 6805 8456 10168 96
Size: 205 859 48 1651 1712 138
Fragments arranged by size:
1857 1712 1651 1443 859 483 312 274
232 222 205 181 170 167 138 80
51 49 48 33 24 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3404 3436 10170 10202 3404
Size: 32 6734 32 3412
Fragments arranged by size:
6734 3412 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10210
XbaI T'CTAG_A
Cuts at: 7546 7546
Size: 10210
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5504 7414 5504
Size: 1910 8300
XhoI C'TCGA_G
Cuts at: 1943 3260 3627 1943
Size: 1317 367 8526
Fragments arranged by size:
8526 1317 367
XmaI C'CCGG_G
Cuts at: 1870 7535 1870
Size: 5665 4545
XmnI GAAnn'nnTTC
Cuts at: 2248 2890 8339 2248
Size: 642 5449 4119
Fragments arranged by size:
5449 4119 642
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8018 276
Size: 53 83 186 936 6484 2468
Fragments arranged by size:
6484 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NruI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BplI BsiWI BsmBI BstXI
FalI FseI FspAI NaeI NgoMIV PacI PmlI PspOMI
RsrII SacII SanDI SfiI SrfI SwaI