Restriction map for
pFN21AE0089
(Circular) (Six-base) MAPSORT of: pFN21AE0089.seq Check: 6370 from: 1 to: 6829
pFN21AE0089 6829 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4639 278
Size: 53 83 186 936 3103 2468
Fragments arranged by size:
3103 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2745 3295 4172 1937
Size: 808 550 877 4594
Fragments arranged by size:
4594 877 808 550
Acc65I G'GTAC_C
Cuts at: 4150 4150
Size: 6829
AclI AA'CG_TT
Cuts at: 1563 4956 5329 1563
Size: 3393 373 3063
Fragments arranged by size:
3393 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2589 2697 4892 6276 6544 1632
Size: 957 108 2195 1384 268 1917
Fragments arranged by size:
2195 1917 1384 957 268 108
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6632
AflIII A'CryG_T
Cuts at: 1203 2277 3281 5728 6705 1203
Size: 1074 1004 2447 977 1327
Fragments arranged by size:
2447 1327 1074 1004 977
AgeI A'CCGG_T
Cuts at: 5660 5660
Size: 6829
AhdI GACnn_n'nnGTC
Cuts at: 2346 5558 2346
Size: 3212 3617
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6829
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3346 1184
Size: 2162 4667
AlwNI CAG_nnn'CTG
Cuts at: 2378 2567 4572 6144 2378
Size: 189 2005 1572 3063
Fragments arranged by size:
3063 2005 1572 189
ApaLI G'TGCA_C
Cuts at: 3890 4886 6042 3890
Size: 996 1156 4677
Fragments arranged by size:
4677 1156 996
ApoI r'AATT_y
Cuts at: 1493 4138 4364 4516 6625 1493
Size: 2645 226 152 2109 1697
Fragments arranged by size:
2645 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5383 160
Size: 872 4351 1606
Fragments arranged by size:
4351 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6829
AvaI C'yCGr_G
Cuts at: 1870 1943 4154 1870
Size: 73 2211 4545
Fragments arranged by size:
4545 2211 73
BamHI G'GATC_C
Cuts at: 2164 4159 4526 2164
Size: 1995 367 4467
Fragments arranged by size:
4467 1995 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4150 5605 618
Size: 325 217 627 2363 1455 1842
Fragments arranged by size:
2363 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2608 4148 1391
Size: 1217 1540 4072
Fragments arranged by size:
4072 1540 1217
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3525 928
Size: 971 1626 4232
Fragments arranged by size:
4232 1626 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5020 5054 1903
Size: 34 3083 34 3678
Fragments arranged by size:
3678 3083 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4721 5931 4721
Size: 1210 5619
BclI T'GATC_A
Cuts at: 1548 2521 1548
Size: 973 5856
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5440 136
Size: 107 122 71 631 605 3768 1525
Fragments arranged by size:
3768 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6824 6824
Size: 6829
BlpI GC'TnA_GC
Cuts at: 4246 4246
Size: 6829
Bme1580I G_kGCm'C
Cuts at: 1790 2092 3894 4890 6046 1790
Size: 302 1802 996 1156 2573
Fragments arranged by size:
2573 1802 1156 996 302
BmgBI CAC'GTC
Cuts at: 1318 2882 1318
Size: 1564 5265
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2314 2500 3532
Size: 416 53 83 399 24 897 186 1032
Cuts at: 3532 4560 5518 442
Size: 1028 958 1753
Fragments arranged by size:
1753 1032 1028 958 897 416 399 186
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2990 1056
Size: 1934 4895
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2670 2670
Size: 6829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3576 5489 1239
Size: 2337 1913 2579
Fragments arranged by size:
2579 2337 1913
Bpu10I CC'TnA_GC
Cuts at: 3906 3944 3906
Size: 38 6791
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2368 3517 4954 5819 6117 2368
Size: 1149 1437 865 298 3080
Fragments arranged by size:
3080 1437 1149 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5492 882
Size: 617 3993 2219
Fragments arranged by size:
3993 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2046 4620 493
Size: 711 842 2574 2702
Fragments arranged by size:
2702 2574 842 711
BsaBI GATnn'nnATC
Cuts at: 2163 6823 2163
Size: 4660 2169
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4636 5018 5653 275
Size: 53 83 186 936 3103 382 635 1451
Fragments arranged by size:
3103 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3069 3315 5262 5660 5934 6081 6492 3069
Size: 246 1947 398 274 147 411 3406
Fragments arranged by size:
3406 1947 411 398 274 246 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2344 2374 2605 2635 3972
Size: 30 165 30 941 30 231 30 1337
Cuts at: 3972 4002 1178
Size: 30 4005
Fragments arranged by size:
4005 1337 941 231 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2352 2883 1186
Size: 1166 531 5132
Fragments arranged by size:
5132 1166 531
BseYI C'CCAG_C
Cuts at: 6032 6528 6032
Size: 496 6333
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6256
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4079 4279 5040 6068 1540
Size: 275 186 2078 200 761 1028 2301
Fragments arranged by size:
2301 2078 1028 761 275 200 186
BsiHKAI G_wGCw'C
Cuts at: 2608 3894 4890 4975 6046 2608
Size: 1286 996 85 1071 3391
Fragments arranged by size:
3391 1286 1071 996 85
BsmI GAATG_Cn'
Cuts at: 4340 4433 4340
Size: 93 6736
BsmBI CGTCTCn'nnnn_
Cuts at: 3213 3213
Size: 6829
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2092 2608 3894 4148 4890 4975 6046
Size: 399 302 516 1286 254 742 85 1071
Cuts at: 6046 1391
Size: 2174
Fragments arranged by size:
2174 1286 1071 742 516 399 302 254
85
BspEI T'CCGG_A
Cuts at: 3069 6492 3069
Size: 3423 3406
BspHI T'CATG_A
Cuts at: 4718 6448 4718
Size: 1730 5099
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4184 844
Size: 3340 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4716 1394
Size: 429 2893 3507
Fragments arranged by size:
3507 2893 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2874 3045 3149 5324 5498 65
Size: 1183 1626 171 104 2175 174 1396
Fragments arranged by size:
2175 1626 1396 1183 174 171 104
BsrFI r'CCGG_y
Cuts at: 1700 1810 5473 5660 1700
Size: 110 3663 187 2869
Fragments arranged by size:
3663 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2282 2950 4082 96
Size: 2186 668 1132 2843
Fragments arranged by size:
2843 2186 1132 668
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6829
BssSI C'ACGA_G
Cuts at: 4889 5901 4889
Size: 1012 5817
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2983 794
Size: 2189 4640
BstBI TT'CG_AA
Cuts at: 5633 5633
Size: 6829
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6829
BstYI r'GATC_y
Cuts at: 1972 2122 2164 2375 4071 4159 4526 4911 4928
Size: 150 42 211 1696 88 367 385 17
Cuts at: 4928 6380 6466 6478 6824 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1696 1452 385 367 346 211 150
88 86 42 17 12
Bsu36I CC'TnA_GG
Cuts at: 2371 2371
Size: 6829
BtgI C'CryG_G
Cuts at: 513 1065 3777 513
Size: 552 2712 3565
Fragments arranged by size:
3565 2712 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6090
Fragments arranged by size:
6090 683 56
BtsI GCAGTG_nn'
Cuts at: 4345 5139 5159 4345
Size: 794 20 6015
Fragments arranged by size:
6015 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6829
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6794
DraI TTT'AAA
Cuts at: 2131 2752 4133 2131
Size: 621 1381 4827
Fragments arranged by size:
4827 1381 621
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5836 809
Size: 507 4520 1802
Fragments arranged by size:
4520 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2057 2297 4076
Size: 54 1425 50 275 18 227 240 1779
Cuts at: 4076 4276 5165 6794 8
Size: 200 889 1629 43
Fragments arranged by size:
1779 1629 1425 889 275 240 227 200
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4076 4276 1537
Size: 275 2264 200 4090
Fragments arranged by size:
4090 2264 275 200
EarI CTCTTCn'nnn_
Cuts at: 1353 2565 2804 3096 3995 4759 1353
Size: 1212 239 292 899 764 3423
Fragments arranged by size:
3423 1212 899 764 292 239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5416 5790 5936 387
Size: 1064 138 3827 374 146 1280
Fragments arranged by size:
3827 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2606 2606
Size: 6829
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2589 2697 3576 4892 5489 6276 6544
Size: 393 957 108 879 1316 597 787 268
Cuts at: 6544 1239
Size: 1524
Fragments arranged by size:
1524 1316 957 879 787 597 393 268
108
EcoRI G'AATT_C
Cuts at: 4138 4516 4138
Size: 378 6451
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2211 2211
Size: 6829
FspI TGC'GCA
Cuts at: 5335 5335
Size: 6829
HaeII r_GCGC'y
Cuts at: 1678 5976 1678
Size: 4298 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2332 2364 3365 3397 3443
Size: 32 135 32 760 32 1001 32 46
Cuts at: 3443 3475 5470 5502 5544 5576 1373
Size: 32 1995 32 42 32 2626
Fragments arranged by size:
2626 1995 1001 760 135 46 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3296 3865 4173 4419 669
Size: 988 281 1358 569 308 246 3079
Fragments arranged by size:
3079 1358 988 569 308 281 246
HindIII A'AGCT_T
Cuts at: 748 3562 748
Size: 2814 4015
HpaI GTT'AAC
Cuts at: 4419 4419
Size: 6829
KpnI G_GTAC'C
Cuts at: 4154 4154
Size: 6829
MfeI C'AATT_G
Cuts at: 4428 4428
Size: 6829
MluI A'CGCG_T
Cuts at: 6705 6705
Size: 6829
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3701 5943 6127 1305
Size: 2396 2242 184 2007
Fragments arranged by size:
2396 2242 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2059 2299 10
Size: 54 1425 343 227 240 4540
Fragments arranged by size:
4540 1425 343 240 227 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4037 4787 5146 5305 518
Size: 648 2871 750 359 159 2042
Fragments arranged by size:
2871 2042 750 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4245 4922 6070 6315 1606
Size: 2639 677 1148 245 2120
Fragments arranged by size:
2639 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3777 513
Size: 552 2712 3565
Fragments arranged by size:
3565 2712 552
NdeI CA'TA_TG
Cuts at: 387 2482 387
Size: 2095 4734
NheI G'CTAG_C
Cuts at: 1052 2986 1052
Size: 1934 4895
NotI GC'GGCC_GC
Cuts at: 4276 4276
Size: 6829
NspI r_CATG'y
Cuts at: 1132 1229 2281 4187 5732 1132
Size: 97 1052 1906 1545 2229
Fragments arranged by size:
2229 1906 1545 1052 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6829
PciI A'CATG_T
Cuts at: 2277 5728 2277
Size: 3451 3378
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6829
PmeI GTTT'AAAC
Cuts at: 4133 4133
Size: 6829
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4898 4930 6411 6443 4898
Size: 32 1481 32 5284
Fragments arranged by size:
5284 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6829
PsiI TTA'TAA
Cuts at: 4399 4399
Size: 6829
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2098 2130 2098
Size: 32 6797
PstI C_TGCA'G
Cuts at: 830 3325 4181 830
Size: 2495 856 3478
Fragments arranged by size:
3478 2495 856
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6829
SacI G_AGCT'C
Cuts at: 2608 2608
Size: 6829
SalI G'TCGA_C
Cuts at: 1936 3294 4171 1936
Size: 1358 877 4594
Fragments arranged by size:
4594 1358 877
SapI GCTCTTCn'nnn_
Cuts at: 2804 2804
Size: 6829
SbfI CC_TGCA'GG
Cuts at: 4181 4181
Size: 6829
ScaI AGT'ACT
Cuts at: 5077 6789 5077
Size: 1712 5117
SfcI C'TryA_G
Cuts at: 826 1045 2352 3321 4177 5312 5993 6184 826
Size: 219 1307 969 856 1135 681 191 1471
Fragments arranged by size:
1471 1307 1135 969 856 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6829
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6829
SmaI CCC'GGG
Cuts at: 1872 4156 1872
Size: 2284 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2383 3496 4933 5834 6096 820
Size: 197 926 440 1113 1437 901 262 1553
Fragments arranged by size:
1553 1437 1113 926 901 440 262 197
SnaBI TAC'GTA
Cuts at: 493 2046 4620 493
Size: 1553 2574 2702
Fragments arranged by size:
2702 2574 1553
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6829
SphI G_CATG'C
Cuts at: 1132 4187 1132
Size: 3055 3774
SspI AAT'ATT
Cuts at: 5 52 2039 2994 3635 4753 5
Size: 47 1987 955 641 1118 2081
Fragments arranged by size:
2081 1987 1118 955 641 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6829
StyI C'CwwG_G
Cuts at: 513 1065 2225 2496 3267 3777 4268 513
Size: 552 1160 271 771 510 491 3074
Fragments arranged by size:
3074 1160 771 552 510 491 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4856 4873 5026 5211 5656 325
Size: 1255 3276 17 153 185 445 1498
Fragments arranged by size:
3276 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2075 2282 2950
Size: 274 80 33 51 1443 98 207 668
Cuts at: 2950 3586 4082 5075 6787 96
Size: 636 496 993 1712 138
Fragments arranged by size:
1712 1443 993 668 636 496 274 207
138 98 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6789 6821 6789
Size: 32 6797
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6829
XbaI T'CTAG_A
Cuts at: 4165 4165
Size: 6829
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2099 3258 2099
Size: 1159 5670
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6829
XmaI C'CCGG_G
Cuts at: 1870 4154 1870
Size: 2284 4545
XmnI GAAnn'nnTTC
Cuts at: 4958 4958
Size: 6829
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4637 276
Size: 53 83 186 936 3103 2468
Fragments arranged by size:
3103 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BstXI BstZ17I DraIII EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI