Restriction map for
pFN21AE0087
(Circular) (Six-base) MAPSORT of: pFN21AE0087.seq Check: 3314 from: 1 to: 7471
pFN21AE0087 7471 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5281 278
Size: 53 83 186 936 3745 2468
Fragments arranged by size:
3745 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4814 1937
Size: 2877 4594
Acc65I G'GTAC_C
Cuts at: 4792 4792
Size: 7471
AclI AA'CG_TT
Cuts at: 1563 5598 5971 1563
Size: 4035 373 3063
Fragments arranged by size:
4035 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2250 2408 4452 5534 6918 7186 1632
Size: 618 158 2044 1082 1384 268 1917
Fragments arranged by size:
2044 1917 1384 1082 618 268 158
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7274
AflIII A'CryG_T
Cuts at: 1203 2127 3708 4076 6370 7347 1203
Size: 924 1581 368 2294 977 1327
Fragments arranged by size:
2294 1581 1327 977 924 368
AgeI A'CCGG_T
Cuts at: 6302 6302
Size: 7471
AhdI GACnn_n'nnGTC
Cuts at: 6200 6200
Size: 7471
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7471
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7471
AlwNI CAG_nnn'CTG
Cuts at: 5214 6786 5214
Size: 1572 5899
ApaLI G'TGCA_C
Cuts at: 5528 6684 5528
Size: 1156 6315
ApoI r'AATT_y
Cuts at: 1493 2241 2489 2566 2704 2941 3371 3642 4780
Size: 748 248 77 138 237 430 271 1138
Cuts at: 4780 5006 5158 7267 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1138 748 430 271 248 237
226 152 138 77
AseI AT'TA_AT
Cuts at: 160 1032 6025 160
Size: 872 4993 1606
Fragments arranged by size:
4993 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7471
AvaI C'yCGr_G
Cuts at: 1870 1943 4796 1870
Size: 73 2853 4545
Fragments arranged by size:
4545 2853 73
BamHI G'GATC_C
Cuts at: 2219 4801 5168 2219
Size: 2582 367 4522
Fragments arranged by size:
4522 2582 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4792 6247 618
Size: 325 217 627 3005 1455 1842
Fragments arranged by size:
3005 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3796 4790 1391
Size: 2405 994 4072
Fragments arranged by size:
4072 2405 994
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2041 2080 2414 3429 4098 4652 4723
Size: 971 142 39 334 1015 669 554 71
Cuts at: 4723 928
Size: 3676
Fragments arranged by size:
3676 1015 971 669 554 334 142 71
39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5662 5696 1903
Size: 34 3725 34 3678
Fragments arranged by size:
3725 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3172 5363 6573 3172
Size: 2191 1210 4070
Fragments arranged by size:
4070 2191 1210
BclI T'GATC_A
Cuts at: 1548 4221 1548
Size: 2673 4798
BfrBI ATG'CAT
Cuts at: 4754 4754
Size: 7471
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6082 136
Size: 107 122 71 631 605 4410 1525
Fragments arranged by size:
4410 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7466 7466
Size: 7471
BlpI GC'TnA_GC
Cuts at: 3544 4888 3544
Size: 1344 6127
Bme1580I G_kGCm'C
Cuts at: 1790 5532 6688 1790
Size: 3742 1156 2573
Fragments arranged by size:
3742 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3711 1318
Size: 2393 5078
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5202 6160 442
Size: 416 53 83 399 24 3785 958 1753
Fragments arranged by size:
3785 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4145 1056
Size: 3089 4382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2748 4111 6131 1239
Size: 1509 1363 2020 2579
Fragments arranged by size:
2579 2020 1509 1363
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5596 6461 6759 5596
Size: 865 298 6308
Fragments arranged by size:
6308 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2842 6134 882
Size: 617 1343 3292 2219
Fragments arranged by size:
3292 2219 1343 617
BsaAI yAC'GTr
Cuts at: 493 1204 5262 493
Size: 711 4058 2702
Fragments arranged by size:
4058 2702 711
BsaBI GATnn'nnATC
Cuts at: 7465 7465
Size: 7471
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5278 5660 6295 275
Size: 53 83 186 936 3745 382 635 1451
Fragments arranged by size:
3745 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5904 6302 6576 6723 7134 5904
Size: 398 274 147 411 6241
Fragments arranged by size:
6241 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2096 2126 2837 2867 1178
Size: 30 165 30 693 30 711 30 5782
Fragments arranged by size:
5782 711 693 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 4384 1186
Size: 931 2267 4273
Fragments arranged by size:
4273 2267 931
BseYI C'CCAG_C
Cuts at: 6674 7170 6674
Size: 496 6975
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2134 1163
Size: 573 398 6500
Fragments arranged by size:
6500 573 398
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4921 5682 6710 1540
Size: 275 186 2920 761 1028 2301
Fragments arranged by size:
2920 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3170 3545 3796 4252 5532 5617 6688 3170
Size: 375 251 456 1280 85 1071 3953
Fragments arranged by size:
3953 1280 1071 456 375 251 85
BsmI GAATG_Cn'
Cuts at: 2897 4982 5075 2897
Size: 2085 93 5293
Fragments arranged by size:
5293 2085 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3170 3545 3796 4252 4790 5532 5617
Size: 399 1380 375 251 456 538 742 85
Cuts at: 5617 6688 1391
Size: 1071 2174
Fragments arranged by size:
2174 1380 1071 742 538 456 399 375
251 85
BspEI T'CCGG_A
Cuts at: 7134 7134
Size: 7471
BspHI T'CATG_A
Cuts at: 5360 7090 5360
Size: 1730 5741
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4826 844
Size: 3982 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5358 1394
Size: 429 3535 3507
Fragments arranged by size:
3535 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2322 2549 3615 5966 6140 65
Size: 1183 1074 227 1066 2351 174 1396
Fragments arranged by size:
2351 1396 1183 1074 1066 227 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6115 6302 1700
Size: 110 4305 187 2869
Fragments arranged by size:
4305 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2443 3705 96
Size: 2347 1262 3862
Fragments arranged by size:
3862 2347 1262
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7471
BssSI C'ACGA_G
Cuts at: 5531 6543 5531
Size: 1012 6459
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7471
BstBI TT'CG_AA
Cuts at: 6275 6275
Size: 7471
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7471
BstYI r'GATC_y
Cuts at: 1972 2219 4801 5168 5553 5570 7022 7108 7120
Size: 247 2582 367 385 17 1452 86 12
Cuts at: 7120 7466 1972
Size: 346 1977
Fragments arranged by size:
2582 1977 1452 385 367 346 247 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6919
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6732
Fragments arranged by size:
6732 683 56
BtsI GCAGTG_nn'
Cuts at: 2826 4987 5781 5801 2826
Size: 2161 794 20 4496
Fragments arranged by size:
4496 2161 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7471
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2487 2522 3429 3464 529
Size: 35 1923 35 907 35 4536
Fragments arranged by size:
4536 1923 907 35 35 35
DraI TTT'AAA
Cuts at: 3379 3520 3700 3970 4540 4775 3379
Size: 141 180 270 570 235 6075
Fragments arranged by size:
6075 570 270 235 180 141
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6478 809
Size: 507 5162 1802
Fragments arranged by size:
5162 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4918 5807 7436
Size: 54 1425 50 275 18 3088 889 1629
Cuts at: 7436 8
Size: 43
Fragments arranged by size:
3088 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4918 1537
Size: 275 3106 4090
Fragments arranged by size:
4090 3106 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3801 5401 1353
Size: 2448 1600 3423
Fragments arranged by size:
3423 2448 1600
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6058 6432 6578 387
Size: 1064 138 4469 374 146 1280
Fragments arranged by size:
4469 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3794 3794
Size: 7471
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2250 2408 2748 4111 4452 5534 6131
Size: 393 618 158 340 1363 341 1082 597
Cuts at: 6131 6918 7186 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1363 1082 787 618 597 393 341
340 268 158
EcoRI G'AATT_C
Cuts at: 3371 4780 5158 3371
Size: 1409 378 5684
Fragments arranged by size:
5684 1409 378
EcoRV GAT'ATC
Cuts at: 4050 4177 4188 4050
Size: 127 11 7333
Fragments arranged by size:
7333 127 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2380 3766 4457 2380
Size: 1386 691 5394
Fragments arranged by size:
5394 1386 691
FspI TGC'GCA
Cuts at: 5977 5977
Size: 7471
HaeII r_GCGC'y
Cuts at: 1678 6618 1678
Size: 4940 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2837 2869 3290 3322 6112
Size: 32 135 32 1265 32 421 32 2790
Cuts at: 6112 6144 6186 6218 1373
Size: 32 42 32 2626
Fragments arranged by size:
2790 2626 1265 421 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2883 4815 5061 669
Size: 988 281 945 1932 246 3079
Fragments arranged by size:
3079 1932 988 945 281 246
HindIII A'AGCT_T
Cuts at: 748 3513 748
Size: 2765 4706
HpaI GTT'AAC
Cuts at: 5061 5061
Size: 7471
KpnI G_GTAC'C
Cuts at: 4796 4796
Size: 7471
MfeI C'AATT_G
Cuts at: 2873 4212 5070 2873
Size: 1339 858 5274
Fragments arranged by size:
5274 1339 858
MluI A'CGCG_T
Cuts at: 7347 7347
Size: 7471
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3170 3873 4214 4610 6585 6769 1305
Size: 1865 703 341 396 1975 184 2007
Fragments arranged by size:
2007 1975 1865 703 396 341 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5649
Fragments arranged by size:
5649 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2793 3046 3218 5429 5788 5947 518
Size: 648 1627 253 172 2211 359 159 2042
Fragments arranged by size:
2211 2042 1627 648 359 253 172 159
MspA1I CmG'CkG
Cuts at: 1606 4887 5564 6712 6957 1606
Size: 3281 677 1148 245 2120
Fragments arranged by size:
3281 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6919
NdeI CA'TA_TG
Cuts at: 387 2194 387
Size: 1807 5664
NheI G'CTAG_C
Cuts at: 1052 4141 1052
Size: 3089 4382
NotI GC'GGCC_GC
Cuts at: 4918 4918
Size: 7471
NsiI A_TGCA'T
Cuts at: 4756 4756
Size: 7471
NspI r_CATG'y
Cuts at: 1132 1229 2131 4450 4498 4829 6374 1132
Size: 97 902 2319 48 331 1545 2229
Fragments arranged by size:
2319 2229 1545 902 331 97 48
PasI CC'CwG_GG
Cuts at: 1348 2071 1348
Size: 723 6748
PciI A'CATG_T
Cuts at: 2127 6370 2127
Size: 4243 3228
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2862 1108
Size: 1754 5717
PfoI T'CCnGG_A
Cuts at: 2736 2736
Size: 7471
PmeI GTTT'AAAC
Cuts at: 4775 4775
Size: 7471
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5540 5572 7053 7085 5540
Size: 32 1481 32 5926
Fragments arranged by size:
5926 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2834 4214 1613
Size: 1221 1380 4870
Fragments arranged by size:
4870 1380 1221
PsiI TTA'TAA
Cuts at: 2298 2981 5041 2298
Size: 683 2060 4728
Fragments arranged by size:
4728 2060 683
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3324 3356 3324
Size: 32 7439
PstI C_TGCA'G
Cuts at: 830 4823 830
Size: 3993 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7471
SacI G_AGCT'C
Cuts at: 3796 3796
Size: 7471
SalI G'TCGA_C
Cuts at: 1936 4813 1936
Size: 2877 4594
SapI GCTCTTCn'nnn_
Cuts at: 3801 3801
Size: 7471
SbfI CC_TGCA'GG
Cuts at: 4823 4823
Size: 7471
ScaI AGT'ACT
Cuts at: 4456 5719 7431 4456
Size: 1263 1712 4496
Fragments arranged by size:
4496 1712 1263
SfcI C'TryA_G
Cuts at: 826 1045 2721 2886 3574 4819 5954 6635 6826
Size: 219 1676 165 688 1245 1135 681 191
Cuts at: 6826 826
Size: 1471
Fragments arranged by size:
1676 1471 1245 1135 688 681 219 191
165
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7471
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7471
SmaI CCC'GGG
Cuts at: 1872 4798 1872
Size: 2926 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5575 6476 6738 820
Size: 197 926 3632 901 262 1553
Fragments arranged by size:
3632 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5262 493
Size: 4769 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7471
SphI G_CATG'C
Cuts at: 1132 4450 4498 4829 1132
Size: 3318 48 331 3774
Fragments arranged by size:
3774 3318 331 48
SspI AAT'ATT
Cuts at: 5 52 5395 5
Size: 47 5343 2081
Fragments arranged by size:
5343 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7471
StyI C'CwwG_G
Cuts at: 513 1065 3590 4910 513
Size: 552 2525 1320 3074
Fragments arranged by size:
3074 2525 1320 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2519 3003 5498 5515 5668 5853 6298
Size: 1255 939 484 2495 17 153 185 445
Cuts at: 6298 325
Size: 1498
Fragments arranged by size:
2495 1498 1255 939 484 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2443 3705 4079
Size: 274 80 33 51 1443 466 1262 374
Cuts at: 4079 4454 5717 7429 96
Size: 375 1263 1712 138
Fragments arranged by size:
1712 1443 1263 1262 466 375 374 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2096 2128 4228 4260 7431 7463 2096
Size: 32 2100 32 3171 32 2104
Fragments arranged by size:
3171 2104 2100 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7471
XbaI T'CTAG_A
Cuts at: 3481 4355 4807 3481
Size: 874 452 6145
Fragments arranged by size:
6145 874 452
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7471
XmaI C'CCGG_G
Cuts at: 1870 4796 1870
Size: 2926 4545
XmnI GAAnn'nnTTC
Cuts at: 5600 5600
Size: 7471
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5279 276
Size: 53 83 186 936 3745 2468
Fragments arranged by size:
3745 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoO109I FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PvuII
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI