Restriction map for
pFN21AE0083
(Circular) (Six-base) MAPSORT of: pFN21AE0083.seq Check: 78 from: 1 to: 6280
pFN21AE0083 6280 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4090 278
Size: 53 83 186 936 2554 2468
Fragments arranged by size:
2554 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3623 1937
Size: 1686 4594
Acc65I G'GTAC_C
Cuts at: 3601 3601
Size: 6280
AclI AA'CG_TT
Cuts at: 1563 4407 4780 1563
Size: 2844 373 3063
Fragments arranged by size:
3063 2844 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2777 3051 4343 5727 5995 1632
Size: 1145 274 1292 1384 268 1917
Fragments arranged by size:
1917 1384 1292 1145 274 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6083
AflIII A'CryG_T
Cuts at: 1203 2925 5179 6156 1203
Size: 1722 2254 977 1327
Fragments arranged by size:
2254 1722 1327 977
AgeI A'CCGG_T
Cuts at: 5111 5111
Size: 6280
AhdI GACnn_n'nnGTC
Cuts at: 5009 5009
Size: 6280
AleI CACnn'nnGTG
Cuts at: 1166 3321 1166
Size: 2155 4125
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2142 1184
Size: 958 5322
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3369 3401 3369
Size: 32 6248
AlwNI CAG_nnn'CTG
Cuts at: 3490 4023 5595 3490
Size: 533 1572 4175
Fragments arranged by size:
4175 1572 533
ApaLI G'TGCA_C
Cuts at: 4337 5493 4337
Size: 1156 5124
ApoI r'AATT_y
Cuts at: 1493 3525 3589 3815 3967 6076 1493
Size: 2032 64 226 152 2109 1697
Fragments arranged by size:
2109 2032 1697 226 152 64
AseI AT'TA_AT
Cuts at: 160 1032 2390 4834 160
Size: 872 1358 2444 1606
Fragments arranged by size:
2444 1606 1358 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6280
AvaI C'yCGr_G
Cuts at: 1870 1943 2548 3605 1870
Size: 73 605 1057 4545
Fragments arranged by size:
4545 1057 605 73
AvrII C'CTAG_G
Cuts at: 2503 2503
Size: 6280
BamHI G'GATC_C
Cuts at: 3610 3977 3610
Size: 367 5913
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3601 5056 618
Size: 325 217 627 1814 1455 1842
Fragments arranged by size:
1842 1814 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2547 3599 1391
Size: 1156 1052 4072
Fragments arranged by size:
4072 1156 1052
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3501 928
Size: 971 1602 3707
Fragments arranged by size:
3707 1602 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3231 3265 4471 4505 1903
Size: 34 1294 34 1206 34 3678
Fragments arranged by size:
3678 1294 1206 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4172 5382 4172
Size: 1210 5070
BclI T'GATC_A
Cuts at: 1548 2311 1548
Size: 763 5517
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4891 136
Size: 107 122 71 631 605 3219 1525
Fragments arranged by size:
3219 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6275 6275
Size: 6280
BlpI GC'TnA_GC
Cuts at: 2849 3697 2849
Size: 848 5432
Bme1580I G_kGCm'C
Cuts at: 1790 3479 4341 5497 1790
Size: 1689 862 1156 2573
Fragments arranged by size:
2573 1689 1156 862
BmgBI CAC'GTC
Cuts at: 1318 2246 1318
Size: 928 5352
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2128 2462 4011
Size: 416 53 83 399 24 711 334 1549
Cuts at: 4011 4969 442
Size: 958 1753
Fragments arranged by size:
1753 1549 958 711 416 399 334 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6280
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 3322 4940 1239
Size: 794 1289 1618 2579
Fragments arranged by size:
2579 1618 1289 794
Bpu10I CC'TnA_GC
Cuts at: 3480 3480
Size: 6280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4405 5270 5568 4405
Size: 865 298 5117
Fragments arranged by size:
5117 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4943 882
Size: 617 3444 2219
Fragments arranged by size:
3444 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4071 493
Size: 711 2867 2702
Fragments arranged by size:
2867 2702 711
BsaBI GATnn'nnATC
Cuts at: 2988 6274 2988
Size: 3286 2994
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4087 4469 5104 275
Size: 53 83 186 936 2554 382 635 1451
Fragments arranged by size:
2554 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4713 5111 5385 5532 5943 4713
Size: 398 274 147 411 5050
Fragments arranged by size:
5050 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6055
Fragments arranged by size:
6055 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2340 2606 3180 3277 3524 1186
Size: 1154 266 574 97 247 3942
Fragments arranged by size:
3942 1154 574 266 247 97
BseYI C'CCAG_C
Cuts at: 5483 5979 5483
Size: 496 5784
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2878 3537 1163
Size: 573 1142 659 3906
Fragments arranged by size:
3906 1142 659 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3730 4491 5519 1540
Size: 275 186 1729 761 1028 2301
Fragments arranged by size:
2301 1729 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2547 2694 4341 4426 5497 2547
Size: 147 1647 85 1071 3330
Fragments arranged by size:
3330 1647 1071 147 85
BsmI GAATG_Cn'
Cuts at: 2708 2868 3791 3884 2708
Size: 160 923 93 5104
Fragments arranged by size:
5104 923 160 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2547 2694 3479 3599 4341 4426 5497
Size: 399 757 147 785 120 742 85 1071
Cuts at: 5497 1391
Size: 2174
Fragments arranged by size:
2174 1071 785 757 742 399 147 120
85
BspEI T'CCGG_A
Cuts at: 5943 5943
Size: 6280
BspHI T'CATG_A
Cuts at: 4169 5899 4169
Size: 1730 4550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2844 3635 844
Size: 2000 791 3489
Fragments arranged by size:
3489 2000 791
BsrBI CCG'CTC
Cuts at: 1394 1823 2017 4167 1394
Size: 429 194 2150 3507
Fragments arranged by size:
3507 2150 429 194
BsrDI GCAATG_nn'
Cuts at: 65 1248 2167 4775 4949 65
Size: 1183 919 2608 174 1396
Fragments arranged by size:
2608 1396 1183 919 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4924 5111 1700
Size: 110 3114 187 2869
Fragments arranged by size:
3114 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6280
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6280
BssSI C'ACGA_G
Cuts at: 4340 5352 4340
Size: 1012 5268
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2928 794
Size: 2134 4146
BstBI TT'CG_AA
Cuts at: 2152 2821 2860 5084 2152
Size: 669 39 2224 3348
Fragments arranged by size:
3348 2224 669 39
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6280
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3323 2011
Size: 1312 4968
BstYI r'GATC_y
Cuts at: 1972 2485 3610 3977 4362 4379 5831 5917 5929
Size: 513 1125 367 385 17 1452 86 12
Cuts at: 5929 6275 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1125 513 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2076 3372 513
Size: 552 939 72 1296 3421
Fragments arranged by size:
3421 1296 939 552 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5541
Fragments arranged by size:
5541 683 56
BtsI GCAGTG_nn'
Cuts at: 3796 4590 4610 3796
Size: 794 20 5466
Fragments arranged by size:
5466 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6280
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6245
DraI TTT'AAA
Cuts at: 2517 3584 2517
Size: 1067 5213
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5287 809
Size: 507 3971 1802
Fragments arranged by size:
3971 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2781 3727 4616
Size: 54 1425 50 275 18 951 946 889
Cuts at: 4616 6245 8
Size: 1629 43
Fragments arranged by size:
1629 1425 951 946 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3727 1537
Size: 275 1915 4090
Fragments arranged by size:
4090 1915 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2433 4210 1353
Size: 1080 1777 3423
Fragments arranged by size:
3423 1777 1080
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4867 5241 5387 387
Size: 1064 138 3278 374 146 1280
Fragments arranged by size:
3278 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2545 2545
Size: 6280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2777 3051 3322 4343 4940 5727
Size: 393 401 744 274 271 1021 597 787
Cuts at: 5727 5995 1239
Size: 268 1524
Fragments arranged by size:
1524 1021 787 744 597 401 393 274
271 268
EcoO109I rG'GnC_Cy
Cuts at: 3487 3487
Size: 6280
EcoRI G'AATT_C
Cuts at: 3589 3967 3589
Size: 378 5902
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2731 2922 2731
Size: 191 6089
FspI TGC'GCA
Cuts at: 4786 4786
Size: 6280
HaeII r_GCGC'y
Cuts at: 1678 3286 5427 1678
Size: 1608 2141 2531
Fragments arranged by size:
2531 2141 1608
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4921 4953 4995 5027 1373
Size: 32 135 32 3349 32 42 32 2626
Fragments arranged by size:
3349 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2262 3624 3870 669
Size: 988 281 324 1362 246 3079
Fragments arranged by size:
3079 1362 988 324 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6280
HpaI GTT'AAC
Cuts at: 3870 3870
Size: 6280
KpnI G_GTAC'C
Cuts at: 3605 3605
Size: 6280
MfeI C'AATT_G
Cuts at: 3879 3879
Size: 6280
MluI A'CGCG_T
Cuts at: 6156 6156
Size: 6280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3231 5394 5578 1305
Size: 1926 2163 184 2007
Fragments arranged by size:
2163 2007 1926 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2783 10
Size: 54 1425 343 951 3507
Fragments arranged by size:
3507 1425 951 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2299 2697 3321 4238 4597 4756 518
Size: 648 1133 398 624 917 359 159 2042
Fragments arranged by size:
2042 1133 917 648 624 398 359 159
MspA1I CmG'CkG
Cuts at: 1606 3303 3696 4373 5521 5766 1606
Size: 1697 393 677 1148 245 2120
Fragments arranged by size:
2120 1697 1148 677 393 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3372 513
Size: 552 939 1368 3421
Fragments arranged by size:
3421 1368 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6280
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6280
NotI GC'GGCC_GC
Cuts at: 3727 3727
Size: 6280
NspI r_CATG'y
Cuts at: 1132 1229 2929 3638 5183 1132
Size: 97 1700 709 1545 2229
Fragments arranged by size:
2229 1700 1545 709 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6280
PciI A'CATG_T
Cuts at: 2925 5179 2925
Size: 2254 4026
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6280
PmeI GTTT'AAAC
Cuts at: 3584 3584
Size: 6280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4349 4381 5862 5894 4349
Size: 32 1481 32 4735
Fragments arranged by size:
4735 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6280
PsiI TTA'TAA
Cuts at: 3850 3850
Size: 6280
PstI C_TGCA'G
Cuts at: 830 3235 3632 830
Size: 2405 397 3478
Fragments arranged by size:
3478 2405 397
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6280
SacI G_AGCT'C
Cuts at: 2547 2547
Size: 6280
SalI G'TCGA_C
Cuts at: 1936 3622 1936
Size: 1686 4594
SapI GCTCTTCn'nnn_
Cuts at: 2433 2433
Size: 6280
SbfI CC_TGCA'GG
Cuts at: 3632 3632
Size: 6280
ScaI AGT'ACT
Cuts at: 2055 4528 6240 2055
Size: 2473 1712 2095
Fragments arranged by size:
2473 2095 1712
SfcI C'TryA_G
Cuts at: 826 1045 3231 3628 4763 5444 5635 826
Size: 219 2186 397 1135 681 191 1471
Fragments arranged by size:
2186 1471 1135 681 397 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6280
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6280
SmaI CCC'GGG
Cuts at: 1872 3607 1872
Size: 1735 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4384 5285 5547 820
Size: 197 926 2441 901 262 1553
Fragments arranged by size:
2441 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4071 493
Size: 3578 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6280
SphI G_CATG'C
Cuts at: 1132 3638 1132
Size: 2506 3774
SspI AAT'ATT
Cuts at: 5 52 2107 2471 4204 5
Size: 47 2055 364 1733 2081
Fragments arranged by size:
2081 2055 1733 364 47
StuI AGG'CCT
Cuts at: 1517 3335 1517
Size: 1818 4462
StyI C'CwwG_G
Cuts at: 513 1065 2004 2503 2978 3372 3719 513
Size: 552 939 499 475 394 347 3074
Fragments arranged by size:
3074 939 552 499 475 394 347
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2017 3513 4307 4324 4477 4662 5107
Size: 1255 437 1496 794 17 153 185 445
Cuts at: 5107 325
Size: 1498
Fragments arranged by size:
1498 1496 1255 794 445 437 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2053 3106 4526
Size: 274 80 33 51 1443 76 1053 1420
Cuts at: 4526 6238 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1420 1053 274 138 80 76
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2799 2831 6240 6272 2799
Size: 32 3409 32 2807
Fragments arranged by size:
3409 2807 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6280
XbaI T'CTAG_A
Cuts at: 3616 3616
Size: 6280
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6280
XmaI C'CCGG_G
Cuts at: 1870 3605 1870
Size: 1735 4545
XmnI GAAnn'nnTTC
Cuts at: 2740 4409 2740
Size: 1669 4611
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4088 276
Size: 53 83 186 936 2554 2468
Fragments arranged by size:
2554 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI