Restriction map for
pFN21AE0076
(Circular) (Six-base) MAPSORT of: pFN21AE0076.seq Check: 6349 from: 1 to: 9514
pFN21AE0076 9514 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4255 4286 6215 4255
Size: 31 1929 7554
Fragments arranged by size:
7554 1929 31
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3033 3849 7324 278
Size: 53 83 186 936 1497 816 3475 2468
Fragments arranged by size:
3475 2468 1497 936 816 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4197 6857 1937
Size: 2260 2660 4594
Fragments arranged by size:
4594 2660 2260
Acc65I G'GTAC_C
Cuts at: 5212 6405 6699 6835 5212
Size: 1193 294 136 7891
Fragments arranged by size:
7891 1193 294 136
AclI AA'CG_TT
Cuts at: 1563 7641 8014 1563
Size: 6078 373 3063
Fragments arranged by size:
6078 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3587 4060 4794 5101 7577 8961 9229 1632
Size: 1955 473 734 307 2476 1384 268 1917
Fragments arranged by size:
2476 1955 1917 1384 734 473 307 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9317
AflIII A'CryG_T
Cuts at: 1203 8413 9390 1203
Size: 7210 977 1327
Fragments arranged by size:
7210 1327 977
AgeI A'CCGG_T
Cuts at: 2302 8345 2302
Size: 6043 3471
AhdI GACnn_n'nnGTC
Cuts at: 8243 8243
Size: 9514
AleI CACnn'nnGTG
Cuts at: 1166 2427 1166
Size: 1261 8253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2928 1184
Size: 1744 7770
AlwNI CAG_nnn'CTG
Cuts at: 2026 2416 2690 2830 2953 3064 3722 3784 4133
Size: 390 274 140 123 111 658 62 349
Cuts at: 4133 4301 5234 5252 6608 7257 8829 2026
Size: 168 933 18 1356 649 1572 2711
Fragments arranged by size:
2711 1572 1356 933 658 649 390 349
274 168 140 123 111 62 18
ApaI G_GGCC'C
Cuts at: 4148 4336 5087 5137 5808 6746 6768 4148
Size: 188 751 50 671 938 22 6894
Fragments arranged by size:
6894 938 751 671 188 50 22
ApaLI G'TGCA_C
Cuts at: 3941 4464 7571 8727 3941
Size: 523 3107 1156 4728
Fragments arranged by size:
4728 3107 1156 523
ApoI r'AATT_y
Cuts at: 1493 6823 7049 7201 9310 1493
Size: 5330 226 152 2109 1697
Fragments arranged by size:
5330 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8068 160
Size: 872 7036 1606
Fragments arranged by size:
7036 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9514
AvaI C'yCGr_G
Cuts at: 1870 1943 2795 2810 2876 3125 3230 4147 4398
Size: 73 852 15 66 249 105 917 251
Cuts at: 4398 5291 6122 6785 6839 1870
Size: 893 831 663 54 4545
Fragments arranged by size:
4545 917 893 852 831 663 251 249
105 73 66 54 15
AvrII C'CTAG_G
Cuts at: 2615 2615
Size: 9514
BamHI G'GATC_C
Cuts at: 6844 7211 6844
Size: 367 9147
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2220 4167 4267 4347 4610
Size: 325 217 627 433 1947 100 80 263
Cuts at: 4610 4846 5212 5222 6074 6405 6699 6835 8290
Size: 236 366 10 852 331 294 136 1455
Cuts at: 8290 618
Size: 1842
Fragments arranged by size:
1947 1842 1455 852 627 433 366 331
325 294 263 236 217 136 100 80
10
BanII G_rGCy'C
Cuts at: 1391 2454 3054 3090 3555 4148 4336 4488 5087
Size: 1063 600 36 465 593 188 152 599
Cuts at: 5087 5137 5421 5808 6276 6746 6768 6833 1391
Size: 50 284 387 468 470 22 65 4072
Fragments arranged by size:
4072 1063 600 599 593 470 468 465
387 284 188 152 65 50 36 22
BbeI G_GCGC'C
Cuts at: 2224 2224
Size: 9514
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3483 3595 928
Size: 971 1584 112 6847
Fragments arranged by size:
6847 1584 971 112
BbvCI CC'TCA_GC
Cuts at: 2823 4858 2823
Size: 2035 7479
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 7705 7739 1903
Size: 34 50 34 5684 34 3678
Fragments arranged by size:
5684 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7406 8616 7406
Size: 1210 8304
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9514
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3067 3265 3725
Size: 107 122 71 631 605 1395 198 460
Cuts at: 3725 3740 3860 5335 5369 8125 136
Size: 15 120 1475 34 2756 1525
Fragments arranged by size:
2756 1525 1475 1395 631 605 460 198
122 120 107 71 34 15
BglII A'GATC_T
Cuts at: 9509 9509
Size: 9514
BlpI GC'TnA_GC
Cuts at: 3549 4487 5845 6418 6931 3549
Size: 938 1358 573 513 6132
Fragments arranged by size:
6132 1358 938 573 513
Bme1580I G_kGCm'C
Cuts at: 1790 3072 3945 4148 4170 4272 4336 4350 4468
Size: 1282 873 203 22 102 64 14 118
Cuts at: 4468 4613 4639 4851 5087 5137 5225 5808 6395
Size: 145 26 212 236 50 88 583 587
Cuts at: 6395 6511 6746 6768 7575 8731 1790
Size: 116 235 22 807 1156 2573
Fragments arranged by size:
2573 1282 1156 873 807 587 583 236
235 212 203 145 118 116 102 88
64 50 26 22 22 14
BmgBI CAC'GTC
Cuts at: 1318 5410 1318
Size: 4092 5422
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2511 3374 3918
Size: 416 53 83 399 24 1094 863 544
Cuts at: 3918 4218 4266 4425 4688 4906 5368 5388 5512
Size: 300 48 159 263 218 462 20 124
Cuts at: 5512 5811 6230 7245 8203 442
Size: 299 419 1015 958 1753
Fragments arranged by size:
1753 1094 1015 958 863 544 462 419
416 399 300 299 263 218 159 124
83 53 48 24 20
BmtI G_CTAG'C
Cuts at: 1056 6525 1056
Size: 5469 4045
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2767 5509 5521 2767
Size: 2742 12 6760
Fragments arranged by size:
6760 2742 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2909 2930 3068 4442 4785 5627 6275 6396
Size: 1670 21 138 1374 343 842 648 121
Cuts at: 6396 8174 1239
Size: 1778 2579
Fragments arranged by size:
2579 1778 1670 1374 842 648 343 138
121 21
Bpu10I CC'TnA_GC
Cuts at: 2604 2610 2691 2823 3509 4858 6335 2604
Size: 6 81 132 686 1349 1477 5783
Fragments arranged by size:
5783 1477 1349 686 132 81 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3517 5557 6263 7639 8504 8802 3517
Size: 2040 706 1376 865 298 4229
Fragments arranged by size:
4229 2040 1376 865 706 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3346 3391 4823 5480 6012 8177 882
Size: 617 1847 45 1432 657 532 2165 2219
Fragments arranged by size:
2219 2165 1847 1432 657 617 532 45
BsaAI yAC'GTr
Cuts at: 493 1204 7305 493
Size: 711 6101 2702
Fragments arranged by size:
6101 2702 711
BsaBI GATnn'nnATC
Cuts at: 2381 9508 2381
Size: 7127 2387
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2221 2727 3030 3222
Size: 53 83 186 936 688 506 303 192
Cuts at: 3222 3816 3846 6177 7321 7703 8338 275
Size: 594 30 2331 1144 382 635 1451
Fragments arranged by size:
2331 1451 1144 936 688 635 594 506
382 303 192 186 83 53 30
BsaWI w'CCGG_w
Cuts at: 2302 7947 8345 8619 8766 9177 2302
Size: 5645 398 274 147 411 2639
Fragments arranged by size:
5645 2639 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2159 2189 6482 6512 1178
Size: 30 165 30 756 30 4293 30 4180
Fragments arranged by size:
4293 4180 756 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2500 2561 3297 4540 5251 5254 5257 5260
Size: 1314 61 736 1243 711 3 3 3
Cuts at: 5260 5263 1186
Size: 3 5437
Fragments arranged by size:
5437 1314 1243 736 711 61 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2210 2334 2582 2628 2772 2838 3054 3457 3529
Size: 124 248 46 144 66 216 403 72
Cuts at: 3529 3670 3795 4133 4329 4647 4718 5039 6107
Size: 141 125 338 196 318 71 321 1068
Cuts at: 6107 6355 6380 8717 9213 2210
Size: 248 25 2337 496 2511
Fragments arranged by size:
2511 2337 1068 496 403 338 321 318
248 248 216 196 144 141 125 124
72 71 66 46 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2071 2390 2457 4010 1163
Size: 573 335 319 67 1553 6667
Fragments arranged by size:
6667 1553 573 335 319 67
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2307 3649 3711 6964 7725 8753
Size: 275 186 306 1342 62 3253 761 1028
Cuts at: 8753 1540
Size: 2301
Fragments arranged by size:
3253 2301 1342 1028 761 306 275 186
62
BsiHKAI G_wGCw'C
Cuts at: 3110 3902 3945 4468 4488 5801 6121 7575 7660
Size: 792 43 523 20 1313 320 1454 85
Cuts at: 7660 8731 3110
Size: 1071 3893
Fragments arranged by size:
3893 1454 1313 1071 792 523 320 85
43 20
BsmI GAATG_Cn'
Cuts at: 7025 7118 7025
Size: 93 9421
BsmBI CGTCTCn'nnnn_
Cuts at: 2304 4222 2304
Size: 1918 7596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2454 3054 3072 3090 3110 3555 3902
Size: 399 664 600 18 18 20 445 347
Cuts at: 3902 3945 4148 4170 4272 4336 4350 4468 4488
Size: 43 203 22 102 64 14 118 20
Cuts at: 4488 4613 4639 4851 5087 5137 5225 5421 5801
Size: 125 26 212 236 50 88 196 380
Cuts at: 5801 5808 6121 6276 6395 6511 6746 6768 6833
Size: 7 313 155 119 116 235 22 65
Cuts at: 6833 7575 7660 8731 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 664 600 445 399 380
347 313 236 235 212 203 196 155
125 119 118 116 102 88 85 65
64 50 43 26 22 22 20 20
18 18 14 7
BspEI T'CCGG_A
Cuts at: 9177 9177
Size: 9514
BspHI T'CATG_A
Cuts at: 2385 7403 9133 2385
Size: 5018 1730 2766
Fragments arranged by size:
5018 2766 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2364 2424 3672 4255 4286 4914 6215 6357
Size: 1520 60 1248 583 31 628 1301 142
Cuts at: 6357 6869 844
Size: 512 3489
Fragments arranged by size:
3489 1520 1301 1248 628 583 512 142
60 31
BsrBI CCG'CTC
Cuts at: 1394 1823 2551 2578 2875 4872 5860 6753 7401
Size: 429 728 27 297 1997 988 893 648
Cuts at: 7401 1394
Size: 3507
Fragments arranged by size:
3507 1997 988 893 728 648 429 297
27
BsrDI GCAATG_nn'
Cuts at: 65 1248 8009 8183 65
Size: 1183 6761 174 1396
Fragments arranged by size:
6761 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2302 3189 4514 5057 5530 6179 8158
Size: 110 492 887 1325 543 473 649 1979
Cuts at: 8158 8345 1700
Size: 187 2869
Fragments arranged by size:
2869 1979 1325 887 649 543 492 473
187 110
BsrGI T'GTAC_A
Cuts at: 96 2431 96
Size: 2335 7179
BssHII G'CGCG_C
Cuts at: 1925 4754 1925
Size: 2829 6685
BssSI C'ACGA_G
Cuts at: 7574 8586 7574
Size: 1012 8502
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3988 4995 5252 5642 6608 794
Size: 3194 1007 257 390 966 3700
Fragments arranged by size:
3700 3194 1007 966 390 257
BstBI TT'CG_AA
Cuts at: 8318 8318
Size: 9514
BstEII G'GTnAC_C
Cuts at: 1184 3137 1184
Size: 1953 7561
BstXI CCAn_nnnn'nTGG
Cuts at: 3268 3890 4420 4696 6259 3268
Size: 622 530 276 1563 6523
Fragments arranged by size:
6523 1563 622 530 276
BstYI r'GATC_y
Cuts at: 1972 3177 3444 6844 7211 7596 7613 9065 9151
Size: 1205 267 3400 367 385 17 1452 86
Cuts at: 9151 9163 9509 1972
Size: 12 346 1977
Fragments arranged by size:
3400 1977 1452 1205 385 367 346 267
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2544 3132 4435 4471 5776 2544
Size: 588 1303 36 1305 6282
Fragments arranged by size:
6282 1305 1303 588 36
BtgI C'CryG_G
Cuts at: 513 1065 2261 2658 2670 3261 3738 4122 4342
Size: 552 1196 397 12 591 477 384 220
Cuts at: 4342 4441 4863 5701 6165 6589 6768 513
Size: 99 422 838 464 424 179 3259
Fragments arranged by size:
3259 1196 838 591 552 477 464 424
422 397 384 220 179 99 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2790 487
Size: 683 56 1564 7211
Fragments arranged by size:
7211 1564 683 56
BtsI GCAGTG_nn'
Cuts at: 3070 4707 5020 5978 6094 7030 7824 7844 3070
Size: 1637 313 958 116 936 794 20 4740
Fragments arranged by size:
4740 1637 958 936 794 313 116 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9514
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 6154 6189 529
Size: 35 5590 35 3854
Fragments arranged by size:
5590 3854 35 35
DraI TTT'AAA
Cuts at: 6818 6818
Size: 9514
DraIII CAC_nnn'GTG
Cuts at: 3614 3926 6590 3614
Size: 312 2664 6538
Fragments arranged by size:
6538 2664 312
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5408 5479 8521 809
Size: 507 4092 71 3042 1802
Fragments arranged by size:
4092 3042 1802 507 71
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2523 2635 2667
Size: 54 1425 50 275 18 693 112 32
Cuts at: 2667 3081 3282 3438 3646 3708 3732 3741 3852
Size: 414 201 156 208 62 24 9 111
Cuts at: 3852 4033 4053 4119 4366 4659 5874 6082 6242
Size: 181 20 66 247 293 1215 208 160
Cuts at: 6242 6961 7850 9479 8
Size: 719 889 1629 43
Fragments arranged by size:
1629 1425 1215 889 719 693 414 293
275 247 208 208 201 181 160 156
112 111 66 62 54 50 43 32
24 20 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 3646 3708 6961 1537
Size: 275 1834 62 3253 4090
Fragments arranged by size:
4090 3253 1834 275 62
EarI CTCTTCn'nnn_
Cuts at: 1353 4514 6228 7444 1353
Size: 3161 1714 1216 3423
Fragments arranged by size:
3423 3161 1714 1216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3208 3481 3601 3787 4371 4739
Size: 1064 138 1619 273 120 186 584 368
Cuts at: 4739 5725 5749 6788 8101 8475 8621 387
Size: 986 24 1039 1313 374 146 1280
Fragments arranged by size:
1619 1313 1280 1064 1039 986 584 374
368 273 186 146 138 120 24
EcoICRI GAG'CTC
Cuts at: 4486 4486
Size: 9514
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2909 2930 3068 3587 4060 4442 4785
Size: 393 1277 21 138 519 473 382 343
Cuts at: 4785 4794 5101 5627 6275 6396 7577 8174 8961
Size: 9 307 526 648 121 1181 597 787
Cuts at: 8961 9229 1239
Size: 268 1524
Fragments arranged by size:
1524 1277 1181 787 648 597 526 519
473 393 382 343 307 268 138 121
21 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2237 2408 2637 5729 6188 2237
Size: 171 229 3092 459 5563
Fragments arranged by size:
5563 3092 459 229 171
EcoO109I rG'GnC_Cy
Cuts at: 2023 2206 2392 2413 2791 2950 3674 3949 4144
Size: 183 186 21 378 159 724 275 195
Cuts at: 4144 4333 4654 5083 5084 5133 5134 5805 5838
Size: 189 321 429 1 49 1 671 33
Cuts at: 5838 5997 6038 6346 6742 6743 6764 2023
Size: 159 41 308 396 1 21 4773
Fragments arranged by size:
4773 724 671 429 396 378 321 308
275 195 189 186 183 159 159 49
41 33 21 21 1 1 1
EcoRI G'AATT_C
Cuts at: 6823 7201 6823
Size: 378 9136
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3169 3880 5128 6772 3169
Size: 711 1248 1644 5911
Fragments arranged by size:
5911 1644 1248 711
FspI TGC'GCA
Cuts at: 8020 8020
Size: 9514
HaeII r_GCGC'y
Cuts at: 1678 2224 4501 5049 5058 8661 1678
Size: 546 2277 548 9 3603 2531
Fragments arranged by size:
3603 2531 2277 548 546 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2789 2821 3092 3124 3169
Size: 32 135 32 1217 32 271 32 45
Cuts at: 3169 3201 3920 3952 4535 4567 5348 5380 5573
Size: 32 719 32 583 32 781 32 193
Cuts at: 5573 5605 5624 5656 8155 8187 8229 8261 1373
Size: 32 19 32 2499 32 42 32 2626
Fragments arranged by size:
2626 2499 1217 781 719 583 271 193
135 45 42 32 32 32 32 32
32 32 32 32 32 32 32 19
HincII GTy'rAC
Cuts at: 669 1657 1938 5911 6858 7104 669
Size: 988 281 3973 947 246 3079
Fragments arranged by size:
3973 3079 988 947 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9514
HpaI GTT'AAC
Cuts at: 7104 7104
Size: 9514
KasI G'GCGC_C
Cuts at: 2220 2220
Size: 9514
KpnI G_GTAC'C
Cuts at: 5216 6409 6703 6839 5216
Size: 1193 294 136 7891
Fragments arranged by size:
7891 1193 294 136
MfeI C'AATT_G
Cuts at: 7113 7113
Size: 9514
MluI A'CGCG_T
Cuts at: 9390 9390
Size: 9514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2287 2479 2553 5463 5466 5532 8628 8812
Size: 982 192 74 2910 3 66 3096 184
Cuts at: 8812 1305
Size: 2007
Fragments arranged by size:
3096 2910 2007 982 192 184 74 66
3
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2525 2637 3440 3743 3854
Size: 54 1425 343 693 112 803 303 111
Cuts at: 3854 4661 6084 10
Size: 807 1423 3440
Fragments arranged by size:
3440 1425 1423 807 803 693 343 303
112 111 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2427 3428 3743 7472 7831 7990
Size: 648 843 418 1001 315 3729 359 159
Cuts at: 7990 518
Size: 2042
Fragments arranged by size:
3729 2042 1001 843 648 418 359 315
159
MspA1I CmG'CkG
Cuts at: 1606 2254 2292 2374 2776 2986 3562 3698 3731
Size: 648 38 82 402 210 576 136 33
Cuts at: 3731 3912 4133 4651 4713 5771 5873 6151 6317
Size: 181 221 518 62 1058 102 278 166
Cuts at: 6317 6355 6384 6422 6608 6930 7607 8755 9000
Size: 38 29 38 186 322 677 1148 245
Cuts at: 9000 1606
Size: 2120
Fragments arranged by size:
2120 1148 1058 677 648 576 518 402
322 278 245 221 210 186 181 166
136 102 82 62 38 38 38 33
29
NaeI GCC'GGC
Cuts at: 3191 5059 6181 3191
Size: 1868 1122 6524
Fragments arranged by size:
6524 1868 1122
NarI GG'CG_CC
Cuts at: 2221 2221
Size: 9514
NcoI C'CATG_G
Cuts at: 513 1065 3738 4342 4863 6768 513
Size: 552 2673 604 521 1905 3259
Fragments arranged by size:
3259 2673 1905 604 552 521
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9514
NgoMIV G'CCGG_C
Cuts at: 3189 5057 6179 3189
Size: 1868 1122 6524
Fragments arranged by size:
6524 1868 1122
NheI G'CTAG_C
Cuts at: 1052 6521 1052
Size: 5469 4045
NotI GC'GGCC_GC
Cuts at: 3646 6961 3646
Size: 3315 6199
NspI r_CATG'y
Cuts at: 1132 1229 5974 6872 8417 1132
Size: 97 4745 898 1545 2229
Fragments arranged by size:
4745 2229 1545 898 97
PasI CC'CwG_GG
Cuts at: 1348 2020 3064 1348
Size: 672 1044 7798
Fragments arranged by size:
7798 1044 672
PciI A'CATG_T
Cuts at: 8413 8413
Size: 9514
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2026 2953 4286 6590 1108
Size: 918 927 1333 2304 4032
Fragments arranged by size:
4032 2304 1333 927 918
PfoI T'CCnGG_A
Cuts at: 2070 2650 2829 2850 4235 4595 5430 5922 2070
Size: 580 179 21 1385 360 835 492 5662
Fragments arranged by size:
5662 1385 835 580 492 360 179 21
PmeI GTTT'AAAC
Cuts at: 6818 6818
Size: 9514
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7583 7615 9096 9128 7583
Size: 32 1481 32 7969
Fragments arranged by size:
7969 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2206 2392 2413 3674 6038 6346 2206
Size: 186 21 1261 2364 308 5374
Fragments arranged by size:
5374 2364 1261 308 186 21
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9514
PsiI TTA'TAA
Cuts at: 7084 7084
Size: 9514
PspOMI G'GGCC_C
Cuts at: 4144 4332 5083 5133 5804 6742 6764 4144
Size: 188 751 50 671 938 22 6894
Fragments arranged by size:
6894 938 751 671 188 50 22
PspXI vC'TCGA_Gb
Cuts at: 2795 2795
Size: 9514
PstI C_TGCA'G
Cuts at: 830 2421 5000 5248 6189 6322 6604 6684 6866
Size: 1591 2579 248 941 133 282 80 182
Cuts at: 6866 830
Size: 3478
Fragments arranged by size:
3478 2579 1591 941 282 248 182 133
80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9514
PvuII CAG'CTG
Cuts at: 2374 2776 2986 3698 6317 6384 6422 2374
Size: 402 210 712 2619 67 38 5466
Fragments arranged by size:
5466 2619 712 402 210 67 38
SacI G_AGCT'C
Cuts at: 4488 4488
Size: 9514
SalI G'TCGA_C
Cuts at: 1936 6856 1936
Size: 4920 4594
SanDI GG'GwC_CC
Cuts at: 2206 3674 2206
Size: 1468 8046
SapI GCTCTTCn'nnn_
Cuts at: 6228 6228
Size: 9514
SbfI CC_TGCA'GG
Cuts at: 6684 6866 6684
Size: 182 9332
ScaI AGT'ACT
Cuts at: 2084 3839 7762 9474 2084
Size: 1755 3923 1712 2124
Fragments arranged by size:
3923 2124 1755 1712
SfcI C'TryA_G
Cuts at: 826 1045 2417 2492 4453 4996 5244 5953 6185
Size: 219 1372 75 1961 543 248 709 232
Cuts at: 6185 6318 6600 6680 6694 6862 7997 8678 8869
Size: 133 282 80 14 168 1135 681 191
Cuts at: 8869 826
Size: 1471
Fragments arranged by size:
1961 1471 1372 1135 709 681 543 282
248 232 219 191 168 133 80 75
14
SfiI GGCCn_nnn'nGGCC
Cuts at: 3740 3860 5369 3740
Size: 120 1509 7885
Fragments arranged by size:
7885 1509 120
SfoI GGC'GCC
Cuts at: 2222 2222
Size: 9514
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9514
SgrAI Cr'CCGG_yG
Cuts at: 1700 3189 1700
Size: 1489 8025
SmaI CCC'GGG
Cuts at: 1872 3127 4149 4400 6841 1872
Size: 1255 1022 251 2441 4545
Fragments arranged by size:
4545 2441 1255 1022 251
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2795 3496 5572 6278 7618 8519
Size: 197 926 852 701 2076 706 1340 901
Cuts at: 8519 8781 820
Size: 262 1553
Fragments arranged by size:
2076 1553 1340 926 901 852 706 701
262 197
SnaBI TAC'GTA
Cuts at: 493 7305 493
Size: 6812 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9514
SphI G_CATG'C
Cuts at: 1132 6872 1132
Size: 5740 3774
SrfI GCCC'GGGC
Cuts at: 4149 4400 4149
Size: 251 9263
SspI AAT'ATT
Cuts at: 5 52 7438 5
Size: 47 7386 2081
Fragments arranged by size:
7386 2081 47
StuI AGG'CCT
Cuts at: 1517 2156 5108 5352 5372 5570 5735 1517
Size: 639 2952 244 20 198 165 5296
Fragments arranged by size:
5296 2952 639 244 198 165 20
StyI C'CwwG_G
Cuts at: 513 1065 2238 2615 2638 2973 3687 3738 4342
Size: 552 1173 377 23 335 714 51 604
Cuts at: 4342 4683 4863 5217 6085 6540 6768 6953 513
Size: 341 180 354 868 455 228 185 3074
Fragments arranged by size:
3074 1173 868 714 604 552 455 377
354 341 335 228 185 180 51 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2180 2530 3937 3974 4185 4365 4406
Size: 1255 600 350 1407 37 211 180 41
Cuts at: 4406 4496 4642 4898 5053 5531 6104 6174 6178
Size: 90 146 256 155 478 573 70 4
Cuts at: 6178 6725 7541 7558 7711 7896 8341 325
Size: 547 816 17 153 185 445 1498
Fragments arranged by size:
1498 1407 1255 816 600 573 547 478
445 350 256 211 185 180 155 153
146 90 70 41 37 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2082 2431 2496
Size: 274 80 33 51 1443 105 349 65
Cuts at: 2496 3837 7760 9472 96
Size: 1341 3923 1712 138
Fragments arranged by size:
3923 1712 1443 1341 349 274 138 105
80 65 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2159 2191 3910 3942 5653 5685 6359 6391 6482
Size: 32 1719 32 1711 32 674 32 91
Cuts at: 6482 6514 9474 9506 2159
Size: 32 2960 32 2167
Fragments arranged by size:
2960 2167 1719 1711 674 91 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6646 1316
Size: 5330 4184
XbaI T'CTAG_A
Cuts at: 6850 6850
Size: 9514
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3268 5428 6725 3268
Size: 2160 1297 6057
Fragments arranged by size:
6057 2160 1297
XhoI C'TCGA_G
Cuts at: 1943 2795 1943
Size: 852 8662
XmaI C'CCGG_G
Cuts at: 1870 3125 4147 4398 6839 1870
Size: 1255 1022 251 2441 4545
Fragments arranged by size:
4545 2441 1255 1022 251
XmnI GAAnn'nnTTC
Cuts at: 7643 7643
Size: 9514
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3031 3847 7322 276
Size: 53 83 186 936 1497 816 3475 2468
Fragments arranged by size:
3475 2468 1497 936 816 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV
FseI FspAI NruI NsiI PacI PmlI PsrI RsrII
SacII SexAI SwaI