Restriction map for
pFN21AE0072
(Circular) (Six-base) MAPSORT of: pFN21AE0072.seq Check: 7317 from: 1 to: 8725
pFN21AE0072 8725 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5166 5166
Size: 8725
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6535 278
Size: 53 83 186 936 4999 2468
Fragments arranged by size:
4999 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2872 6068 1937
Size: 935 3196 4594
Fragments arranged by size:
4594 3196 935
Acc65I G'GTAC_C
Cuts at: 3739 5107 6046 3739
Size: 1368 939 6418
Fragments arranged by size:
6418 1368 939
AclI AA'CG_TT
Cuts at: 1563 6852 7225 1563
Size: 5289 373 3063
Fragments arranged by size:
5289 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2883 4372 5322 6788 8172 8440 1632
Size: 1251 1489 950 1466 1384 268 1917
Fragments arranged by size:
1917 1489 1466 1384 1251 950 268
AflII C'TTAA_G
Cuts at: 820 1017 4625 820
Size: 197 3608 4920
Fragments arranged by size:
4920 3608 197
AflIII A'CryG_T
Cuts at: 1203 7624 8601 1203
Size: 6421 977 1327
Fragments arranged by size:
6421 1327 977
AgeI A'CCGG_T
Cuts at: 7556 7556
Size: 8725
AhdI GACnn_n'nnGTC
Cuts at: 3379 7454 3379
Size: 4075 4650
AleI CACnn'nnGTG
Cuts at: 1166 2900 5015 1166
Size: 1734 2115 4876
Fragments arranged by size:
4876 2115 1734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5685 5895 1184
Size: 4501 210 4014
Fragments arranged by size:
4501 4014 210
AlwNI CAG_nnn'CTG
Cuts at: 2588 2927 3422 6468 8040 2588
Size: 339 495 3046 1572 3273
Fragments arranged by size:
3273 3046 1572 495 339
ApaLI G'TGCA_C
Cuts at: 6782 7938 6782
Size: 1156 7569
ApoI r'AATT_y
Cuts at: 1493 2199 3309 3635 3827 4651 4947 4969 6034
Size: 706 1110 326 192 824 296 22 1065
Cuts at: 6034 6260 6412 8521 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1110 1065 824 706 326 296
226 192 152 22
AseI AT'TA_AT
Cuts at: 160 1032 4446 7279 160
Size: 872 3414 2833 1606
Fragments arranged by size:
3414 2833 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8725
AvaI C'yCGr_G
Cuts at: 1870 1943 6050 1870
Size: 73 4107 4545
Fragments arranged by size:
4545 4107 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2730 2763 5099 5132 5615 5648 2730
Size: 33 2336 33 483 33 5807
Fragments arranged by size:
5807 2336 483 33 33 33
BamHI G'GATC_C
Cuts at: 6055 6422 6055
Size: 367 8358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2165 3739 4559 4615 5107
Size: 325 217 627 378 1574 820 56 492
Cuts at: 5107 6046 7501 618
Size: 939 1455 1842
Fragments arranged by size:
1842 1574 1455 939 820 627 492 378
325 217 56
BanII G_rGCy'C
Cuts at: 1391 6044 1391
Size: 4653 4072
BbeI G_GCGC'C
Cuts at: 2169 2169
Size: 8725
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 7754
BbvCI CC'TCA_GC
Cuts at: 4035 5704 4035
Size: 1669 7056
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6916 6950 1903
Size: 34 4979 34 3678
Fragments arranged by size:
4979 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2332 2408 2731 4519 6617 7827 2332
Size: 76 323 1788 2098 1210 3230
Fragments arranged by size:
3230 2098 1788 1210 323 76
BclI T'GATC_A
Cuts at: 1548 3784 5860 1548
Size: 2236 2076 4413
Fragments arranged by size:
4413 2236 2076
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7336 136
Size: 107 122 71 631 605 5664 1525
Fragments arranged by size:
5664 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2349 4264 8720 2349
Size: 1915 4456 2354
Fragments arranged by size:
4456 2354 1915
BlpI GC'TnA_GC
Cuts at: 6142 6142
Size: 8725
Bme1580I G_kGCm'C
Cuts at: 1790 6786 7942 1790
Size: 4996 1156 2573
Fragments arranged by size:
4996 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8725
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2834 3496 3797
Size: 416 53 83 399 24 1417 662 301
Cuts at: 3797 5207 5916 6456 7414 442
Size: 1410 709 540 958 1753
Fragments arranged by size:
1753 1417 1410 958 709 662 540 416
399 301 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8725
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3553 3553
Size: 8725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3374 3474 5565 7385 1239
Size: 2135 100 2091 1820 2579
Fragments arranged by size:
2579 2135 2091 1820 100
Bpu10I CC'TnA_GC
Cuts at: 4035 5704 4035
Size: 1669 7056
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5032 5388 6850 7715 8013 5032
Size: 356 1462 865 298 5744
Fragments arranged by size:
5744 1462 865 356 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5196 7388 882
Size: 617 3697 2192 2219
Fragments arranged by size:
3697 2219 2192 617
BsaAI yAC'GTr
Cuts at: 493 1204 6516 493
Size: 711 5312 2702
Fragments arranged by size:
5312 2702 711
BsaBI GATnn'nnATC
Cuts at: 8719 8719
Size: 8725
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2166 6532 6914 7549
Size: 53 83 186 936 633 4366 382 635
Cuts at: 7549 275
Size: 1451
Fragments arranged by size:
4366 1451 936 635 633 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2306 7158 7556 7830 7977 8388 2306
Size: 4852 398 274 147 411 2643
Fragments arranged by size:
4852 2643 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5018 5048 5947 5977 1178
Size: 30 165 30 3615 30 899 30 3926
Fragments arranged by size:
3926 3615 899 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2043 5279 5643 1186
Size: 857 3236 364 4268
Fragments arranged by size:
4268 3236 857 364
BseYI C'CCAG_C
Cuts at: 2518 2581 5136 5288 7928 8424 2518
Size: 63 2555 152 2640 496 2819
Fragments arranged by size:
2819 2640 2555 496 152 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5383 5655 5681 1163
Size: 573 3647 272 26 4207
Fragments arranged by size:
4207 3647 573 272 26
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6175 6936 7964 1540
Size: 275 186 4174 761 1028 2301
Fragments arranged by size:
4174 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 6786 6871 7942 6786
Size: 85 1071 7569
Fragments arranged by size:
7569 1071 85
BsmI GAATG_Cn'
Cuts at: 3364 4228 4726 6236 6329 3364
Size: 864 498 1510 93 5760
Fragments arranged by size:
5760 1510 864 498 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 6044 6786 6871 7942 1391
Size: 399 4254 742 85 1071 2174
Fragments arranged by size:
4254 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 8388 8388
Size: 8725
BspHI T'CATG_A
Cuts at: 3304 6614 8344 3304
Size: 3310 1730 3685
Fragments arranged by size:
3685 3310 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3618 4749 5166 6080 844
Size: 2774 1131 417 914 3489
Fragments arranged by size:
3489 2774 1131 914 417
BsrBI CCG'CTC
Cuts at: 1394 1823 6612 1394
Size: 429 4789 3507
Fragments arranged by size:
4789 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2413 2982 3123 3456 3763 7220 7394
Size: 1183 1165 569 141 333 307 3457 174
Cuts at: 7394 65
Size: 1396
Fragments arranged by size:
3457 1396 1183 1165 569 333 307 174
141
BsrFI r'CCGG_y
Cuts at: 1700 1810 2360 5446 7369 7556 1700
Size: 110 550 3086 1923 187 2869
Fragments arranged by size:
3086 2869 1923 550 187 110
BsrGI T'GTAC_A
Cuts at: 96 2298 96
Size: 2202 6523
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8725
BssSI C'ACGA_G
Cuts at: 6785 7797 6785
Size: 1012 7713
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3575 4553 5738 794
Size: 2781 978 1185 3781
Fragments arranged by size:
3781 2781 1185 978
BstBI TT'CG_AA
Cuts at: 2326 7529 2326
Size: 5203 3522
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8725
BstXI CCAn_nnnn'nTGG
Cuts at: 4087 4087
Size: 8725
BstYI r'GATC_y
Cuts at: 1972 2349 2786 2949 4264 4536 5059 6055 6422
Size: 377 437 163 1315 272 523 996 367
Cuts at: 6422 6807 6824 8276 8362 8374 8720 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1315 996 523 437 385 377
367 346 272 163 86 17 12
BstZ17I GTA'TAC
Cuts at: 2873 2873
Size: 8725
Bsu36I CC'TnA_GG
Cuts at: 5259 5259
Size: 8725
BtgI C'CryG_G
Cuts at: 513 1065 5236 513
Size: 552 4171 4002
Fragments arranged by size:
4171 4002 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5581 487
Size: 683 56 4355 3631
Fragments arranged by size:
4355 3631 683 56
BtsI GCAGTG_nn'
Cuts at: 4590 5191 6241 7035 7055 4590
Size: 601 1050 794 20 6260
Fragments arranged by size:
6260 1050 794 601 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8690
DraI TTT'AAA
Cuts at: 6029 6029
Size: 8725
DraIII CAC_nnn'GTG
Cuts at: 4345 4345
Size: 8725
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7732 809
Size: 507 6416 1802
Fragments arranged by size:
6416 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5422 6172 7061
Size: 54 1425 50 275 18 3592 750 889
Cuts at: 7061 8690 8
Size: 1629 43
Fragments arranged by size:
3592 1629 1425 889 750 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6172 1537
Size: 275 4360 4090
Fragments arranged by size:
4360 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2047 2061 2328 2724 3461 4391 4830 5376
Size: 694 14 267 396 737 930 439 546
Cuts at: 5376 5476 5764 6655 1353
Size: 100 288 891 3423
Fragments arranged by size:
3423 930 891 737 694 546 439 396
288 267 100 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7312 7686 7832 387
Size: 1064 138 5723 374 146 1280
Fragments arranged by size:
5723 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2883 3374 3474 4372 5322 5565 6788
Size: 393 1251 491 100 898 950 243 1223
Cuts at: 6788 7385 8172 8440 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1251 1223 950 898 787 597 491
393 268 243 100
EcoNI CCTnn'n_nnAGG
Cuts at: 2601 2601
Size: 8725
EcoO109I rG'GnC_Cy
Cuts at: 3617 3617
Size: 8725
EcoRI G'AATT_C
Cuts at: 3635 4651 6034 6412 3635
Size: 1016 1383 378 5948
Fragments arranged by size:
5948 1383 1016 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2971 2971
Size: 8725
FspI TGC'GCA
Cuts at: 4460 7231 4460
Size: 2771 5954
HaeII r_GCGC'y
Cuts at: 1678 2169 2367 7872 1678
Size: 491 198 5505 2531
Fragments arranged by size:
5505 2531 491 198
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3165 3197 3365 3397 4335
Size: 32 135 32 1593 32 168 32 938
Cuts at: 4335 4367 4496 4528 5018 5050 5300 5312 5332
Size: 32 129 32 490 32 250 12 20
Cuts at: 5332 5344 7366 7398 7440 7472 1373
Size: 12 2022 32 42 32 2626
Fragments arranged by size:
2626 2022 1593 938 490 250 168 135
129 42 32 32 32 32 32 32
32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 4204 4789 6069 6315 669
Size: 988 281 2266 585 1280 246 3079
Fragments arranged by size:
3079 2266 1280 988 585 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8725
HpaI GTT'AAC
Cuts at: 6315 6315
Size: 8725
KasI G'GCGC_C
Cuts at: 2165 2165
Size: 8725
KpnI G_GTAC'C
Cuts at: 3743 5111 6050 3743
Size: 1368 939 6418
Fragments arranged by size:
6418 1368 939
MfeI C'AATT_G
Cuts at: 4858 5038 6324 4858
Size: 180 1286 7259
Fragments arranged by size:
7259 1286 180
MluI A'CGCG_T
Cuts at: 8601 8601
Size: 8725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3003 4000 7839 8023 1305
Size: 1698 997 3839 184 2007
Fragments arranged by size:
3839 2007 1698 997 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5424 10
Size: 54 1425 343 3592 3311
Fragments arranged by size:
3592 3311 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2900 3006 3251 3464 4085 4217 5015
Size: 648 1734 106 245 213 621 132 798
Cuts at: 5015 5430 5922 6683 7042 7201 518
Size: 415 492 761 359 159 2042
Fragments arranged by size:
2042 1734 798 761 648 621 492 415
359 245 213 159 132 106
MspA1I CmG'CkG
Cuts at: 1606 2585 4550 5140 6141 6818 7966 8211 1606
Size: 979 1965 590 1001 677 1148 245 2120
Fragments arranged by size:
2120 1965 1148 1001 979 677 590 245
NaeI GCC'GGC
Cuts at: 5448 5448
Size: 8725
NarI GG'CG_CC
Cuts at: 2166 2166
Size: 8725
NcoI C'CATG_G
Cuts at: 513 1065 5236 513
Size: 552 4171 4002
Fragments arranged by size:
4171 4002 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8725
NgoMIV G'CCGG_C
Cuts at: 5446 5446
Size: 8725
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8725
NotI GC'GGCC_GC
Cuts at: 6172 6172
Size: 8725
NspI r_CATG'y
Cuts at: 1132 1229 3294 3664 4648 6083 7628 1132
Size: 97 2065 370 984 1435 1545 2229
Fragments arranged by size:
2229 2065 1545 1435 984 370 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8725
PciI A'CATG_T
Cuts at: 7624 7624
Size: 8725
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3857 1108
Size: 2749 5976
PfoI T'CCnGG_A
Cuts at: 2114 3351 2114
Size: 1237 7488
PmeI GTTT'AAAC
Cuts at: 6029 6029
Size: 8725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4986 5018 6794 6826 8307 8339 4986
Size: 32 1776 32 1481 32 5372
Fragments arranged by size:
5372 1776 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8725
PsiI TTA'TAA
Cuts at: 6295 6295
Size: 8725
PstI C_TGCA'G
Cuts at: 830 3418 3913 6077 830
Size: 2588 495 2164 3478
Fragments arranged by size:
3478 2588 2164 495
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8725
PvuII CAG'CTG
Cuts at: 4550 4550
Size: 8725
SalI G'TCGA_C
Cuts at: 1936 6067 1936
Size: 4131 4594
SapI GCTCTTCn'nnn_
Cuts at: 3461 4830 5476 5764 3461
Size: 1369 646 288 6422
Fragments arranged by size:
6422 1369 646 288
SbfI CC_TGCA'GG
Cuts at: 6077 6077
Size: 8725
ScaI AGT'ACT
Cuts at: 2794 5270 6973 8685 2794
Size: 2476 1703 1712 2834
Fragments arranged by size:
2834 2476 1712 1703
SfcI C'TryA_G
Cuts at: 826 1045 2889 3414 3669 3909 4596 5065 5716
Size: 219 1844 525 255 240 687 469 651
Cuts at: 5716 6073 7208 7889 8080 826
Size: 357 1135 681 191 1471
Fragments arranged by size:
1844 1471 1135 687 681 651 525 469
357 255 240 219 191
SfoI GGC'GCC
Cuts at: 2167 2167
Size: 8725
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8725
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8725
SmaI CCC'GGG
Cuts at: 1872 6052 1872
Size: 4180 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4625 5047 5403 6829 7730 7992
Size: 197 926 2682 422 356 1426 901 262
Cuts at: 7992 820
Size: 1553
Fragments arranged by size:
2682 1553 1426 926 901 422 356 262
197
SnaBI TAC'GTA
Cuts at: 493 6516 493
Size: 6023 2702
SpeI A'CTAG_T
Cuts at: 152 4520 152
Size: 4368 4357
SphI G_CATG'C
Cuts at: 1132 3664 6083 1132
Size: 2532 2419 3774
Fragments arranged by size:
3774 2532 2419
SspI AAT'ATT
Cuts at: 5 52 2457 3220 3707 3985 6649 5
Size: 47 2405 763 487 278 2664 2081
Fragments arranged by size:
2664 2405 2081 763 487 278 47
StuI AGG'CCT
Cuts at: 1517 5387 5486 5522 5702 1517
Size: 3870 99 36 180 4540
Fragments arranged by size:
4540 3870 180 99 36
StyI C'CwwG_G
Cuts at: 513 1065 2402 3851 4330 5236 5248 6021 6164
Size: 552 1337 1449 479 906 12 773 143
Cuts at: 6164 513
Size: 3074
Fragments arranged by size:
3074 1449 1337 906 773 552 479 143
12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2853 3515 5302 5818 6752 6769 6922
Size: 1255 1273 662 1787 516 934 17 153
Cuts at: 6922 7107 7552 325
Size: 185 445 1498
Fragments arranged by size:
1787 1498 1273 1255 934 662 516 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2298 2792 4854
Size: 274 80 33 51 1443 321 494 2062
Cuts at: 4854 5268 6971 8683 96
Size: 414 1703 1712 138
Fragments arranged by size:
2062 1712 1703 1443 494 414 321 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2499 2531 8685 8717 2499
Size: 32 6154 32 2507
Fragments arranged by size:
6154 2507 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4529 1316
Size: 3213 5512
XbaI T'CTAG_A
Cuts at: 4831 6061 4831
Size: 1230 7495
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3406 3406
Size: 8725
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8725
XmaI C'CCGG_G
Cuts at: 1870 6050 1870
Size: 4180 4545
XmnI GAAnn'nnTTC
Cuts at: 2199 3219 3846 4152 5913 6854 2199
Size: 1020 627 306 1761 941 4070
Fragments arranged by size:
4070 1761 1020 941 627 306
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6533 276
Size: 53 83 186 936 4999 2468
Fragments arranged by size:
4999 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BfrBI BsiWI BsmBI
EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SrfI SwaI