Restriction map for
pFN21AE0070
(Circular) (Six-base) MAPSORT of: pFN21AE0070.seq Check: 285 from: 1 to: 6385
pFN21AE0070 6385 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2565 2565
Size: 6385
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4195 278
Size: 53 83 186 936 2659 2468
Fragments arranged by size:
2659 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3728 1937
Size: 1791 4594
Acc65I G'GTAC_C
Cuts at: 3706 3706
Size: 6385
AclI AA'CG_TT
Cuts at: 1563 4512 4885 1563
Size: 2949 373 3063
Fragments arranged by size:
3063 2949 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2197 2269 2540 4448 5832 6100 1632
Size: 565 72 271 1908 1384 268 1917
Fragments arranged by size:
1917 1908 1384 565 271 268 72
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6188
AflIII A'CryG_T
Cuts at: 1203 2424 3219 5284 6261 1203
Size: 1221 795 2065 977 1327
Fragments arranged by size:
2065 1327 1221 977 795
AgeI A'CCGG_T
Cuts at: 5216 5216
Size: 6385
AhdI GACnn_n'nnGTC
Cuts at: 5114 5114
Size: 6385
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6385
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2924 3143 1184
Size: 1740 219 4426
Fragments arranged by size:
4426 1740 219
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2660 2692 2660
Size: 32 6353
AlwNI CAG_nnn'CTG
Cuts at: 2437 2887 3365 4128 5700 2437
Size: 450 478 763 1572 3122
Fragments arranged by size:
3122 1572 763 478 450
ApaI G_GGCC'C
Cuts at: 3297 3397 3428 3437 3526 3297
Size: 100 31 9 89 6156
Fragments arranged by size:
6156 100 89 31 9
ApaLI G'TGCA_C
Cuts at: 4442 5598 4442
Size: 1156 5229
ApoI r'AATT_y
Cuts at: 1493 3694 3920 4072 6181 1493
Size: 2201 226 152 2109 1697
Fragments arranged by size:
2201 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3662 4939 160
Size: 872 2630 1277 1606
Fragments arranged by size:
2630 1606 1277 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6385
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2110 2591 2708 2848 3421 3429
Size: 73 93 74 481 117 140 573 8
Cuts at: 3429 3710 1870
Size: 281 4545
Fragments arranged by size:
4545 573 481 281 140 117 93 74
73 8
AvrII C'CTAG_G
Cuts at: 3322 3322
Size: 6385
BamHI G'GATC_C
Cuts at: 3715 4082 3715
Size: 367 6018
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2279 2657 3002 3706 5161
Size: 325 217 627 492 378 345 704 1455
Cuts at: 5161 618
Size: 1842
Fragments arranged by size:
1842 1455 704 627 492 378 345 325
217
BanII G_rGCy'C
Cuts at: 1391 2457 2847 2978 3027 3232 3297 3348 3397
Size: 1066 390 131 49 205 65 51 49
Cuts at: 3397 3428 3437 3526 3679 3704 1391
Size: 31 9 89 153 25 4072
Fragments arranged by size:
4072 1066 390 205 153 131 89 65
51 49 49 31 25 9
BbeI G_GCGC'C
Cuts at: 2661 3006 2661
Size: 345 6040
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2080 928
Size: 971 181 5233
Fragments arranged by size:
5233 971 181
BbvCI CC'TCA_GC
Cuts at: 2009 2009
Size: 6385
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4576 4610 1903
Size: 34 50 34 2555 34 3678
Fragments arranged by size:
3678 2555 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4277 5487 4277
Size: 1210 5175
BclI T'GATC_A
Cuts at: 1548 3606 3615 1548
Size: 2058 9 4318
Fragments arranged by size:
4318 2058 9
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3333 3432 4996
Size: 107 122 71 631 605 1661 99 1564
Cuts at: 4996 136
Size: 1525
Fragments arranged by size:
1661 1564 1525 631 605 122 107 99
71
BglII A'GATC_T
Cuts at: 6380 6380
Size: 6385
BlpI GC'TnA_GC
Cuts at: 2586 2984 3802 2586
Size: 398 818 5169
Fragments arranged by size:
5169 818 398
Bme1580I G_kGCm'C
Cuts at: 1790 3148 3264 3297 3397 3422 3428 3437 3526
Size: 1358 116 33 100 25 6 9 89
Cuts at: 3526 4446 5602 1790
Size: 920 1156 2573
Fragments arranged by size:
2573 1358 1156 920 116 100 89 33
25 9 6
BmgBI CAC'GTC
Cuts at: 1318 2122 3220 1318
Size: 804 1098 4483
Fragments arranged by size:
4483 1098 804
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4116 5074 442
Size: 416 53 83 399 24 2699 958 1753
Fragments arranged by size:
2699 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6385
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2034 2034
Size: 6385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2396 2531 3011 3191 3221 5045 1239
Size: 1157 135 480 180 30 1824 2579
Fragments arranged by size:
2579 1824 1157 480 180 135 30
Bpu10I CC'TnA_GC
Cuts at: 2009 2405 2942 2009
Size: 396 537 5452
Fragments arranged by size:
5452 537 396
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4510 5375 5673 4510
Size: 865 298 5222
Fragments arranged by size:
5222 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3197 5048 882
Size: 617 1698 1851 2219
Fragments arranged by size:
2219 1851 1698 617
BsaAI yAC'GTr
Cuts at: 493 1204 4176 493
Size: 711 2972 2702
Fragments arranged by size:
2972 2702 711
BsaBI GATnn'nnATC
Cuts at: 6379 6379
Size: 6385
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2097 2193 2658 3003
Size: 53 83 186 936 564 96 465 345
Cuts at: 3003 4192 4574 5209 275
Size: 1189 382 635 1451
Fragments arranged by size:
1451 1189 936 635 564 465 382 345
186 96 83 53
BsaWI w'CCGG_w
Cuts at: 4818 5216 5490 5637 6048 4818
Size: 398 274 147 411 5155
Fragments arranged by size:
5155 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2369 2399 2589 2619 2660
Size: 30 165 30 966 30 190 30 41
Cuts at: 2660 2690 3239 3269 1178
Size: 30 549 30 4294
Fragments arranged by size:
4294 966 549 190 165 41 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3454 1186
Size: 2268 4117
BseYI C'CCAG_C
Cuts at: 2052 2904 3484 3525 5588 6084 2052
Size: 852 580 41 2063 496 2353
Fragments arranged by size:
2353 2063 852 580 496 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3026 1163
Size: 573 1290 4522
Fragments arranged by size:
4522 1290 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2722 3835 4596 5624 1540
Size: 275 186 721 1113 761 1028 2301
Fragments arranged by size:
2301 1113 1028 761 721 275 186
BsiHKAI G_wGCw'C
Cuts at: 2037 2111 2373 2457 2581 2847 2859 3027 3036
Size: 74 262 84 124 266 12 168 9
Cuts at: 3036 4446 4531 5602 2037
Size: 1410 85 1071 2820
Fragments arranged by size:
2820 1410 1071 266 262 168 124 85
84 74 12 9
BsmI GAATG_Cn'
Cuts at: 3896 3989 3896
Size: 93 6292
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2111 2373 2457 2581 2847 2859
Size: 399 247 74 262 84 124 266 12
Cuts at: 2859 2978 3027 3036 3148 3232 3264 3297 3348
Size: 119 49 9 112 84 32 33 51
Cuts at: 3348 3397 3422 3428 3437 3526 3679 3704 4446
Size: 49 25 6 9 89 153 25 742
Cuts at: 4446 4531 5602 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 266 262 247 153
124 119 112 89 85 84 84 74
51 49 49 33 32 25 25 12
9 9 6
BspEI T'CCGG_A
Cuts at: 6048 6048
Size: 6385
BspHI T'CATG_A
Cuts at: 2241 4274 6004 2241
Size: 2033 1730 2622
Fragments arranged by size:
2622 2033 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2454 2565 3740 844
Size: 1610 111 1175 3489
Fragments arranged by size:
3489 1610 1175 111
BsrBI CCG'CTC
Cuts at: 1394 1823 3388 4272 1394
Size: 429 1565 884 3507
Fragments arranged by size:
3507 1565 884 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4880 5054 65
Size: 1183 3632 174 1396
Fragments arranged by size:
3632 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2721 3377 3529 5029 5216 1700
Size: 110 911 656 152 1500 187 2869
Fragments arranged by size:
2869 1500 911 656 187 152 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6385
BssHII G'CGCG_C
Cuts at: 1925 2047 2126 2988 3130 1925
Size: 122 79 862 142 5180
Fragments arranged by size:
5180 862 142 122 79
BssSI C'ACGA_G
Cuts at: 3329 4445 5457 3329
Size: 1116 1012 4257
Fragments arranged by size:
4257 1116 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2170 794
Size: 1376 5009
BstBI TT'CG_AA
Cuts at: 3655 5189 3655
Size: 1534 4851
BstEII G'GTnAC_C
Cuts at: 1184 3532 1184
Size: 2348 4037
BstXI CCAn_nnnn'nTGG
Cuts at: 3017 3017
Size: 6385
BstYI r'GATC_y
Cuts at: 1972 3715 4082 4467 4484 5936 6022 6034 6380
Size: 1743 367 385 17 1452 86 12 346
Cuts at: 6380 1972
Size: 1977
Fragments arranged by size:
1977 1743 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2312 2312
Size: 6385
BtgI C'CryG_G
Cuts at: 513 1065 2188 2978 3350 3537 513
Size: 552 1123 790 372 187 3361
Fragments arranged by size:
3361 1123 790 552 372 187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2686 487
Size: 683 56 1460 4186
Fragments arranged by size:
4186 1460 683 56
BtsI GCAGTG_nn'
Cuts at: 2111 3901 4695 4715 2111
Size: 1790 794 20 3781
Fragments arranged by size:
3781 1790 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6385
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6350
DraI TTT'AAA
Cuts at: 3689 3689
Size: 6385
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5392 809
Size: 507 4076 1802
Fragments arranged by size:
4076 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3643 3832 4721
Size: 54 1425 50 275 18 1813 189 889
Cuts at: 4721 6350 8
Size: 1629 43
Fragments arranged by size:
1813 1629 1425 889 275 189 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3832 1537
Size: 275 2020 4090
Fragments arranged by size:
4090 2020 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2180 2504 2864 3155 3336 4315 1353
Size: 827 324 360 291 181 979 3423
Fragments arranged by size:
3423 979 827 360 324 291 181
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2058 2254 4972 5346 5492 387
Size: 1064 138 469 196 2718 374 146 1280
Fragments arranged by size:
2718 1280 1064 469 374 196 146 138
EcoICRI GAG'CTC
Cuts at: 2455 2845 3025 2455
Size: 390 180 5815
Fragments arranged by size:
5815 390 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2197 2269 2396 2531 2540 3011 3191
Size: 393 565 72 127 135 9 471 180
Cuts at: 3191 3221 4448 5045 5832 6100 1239
Size: 30 1227 597 787 268 1524
Fragments arranged by size:
1524 1227 787 597 565 471 393 268
180 135 127 72 30 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2561 2561
Size: 6385
EcoO109I rG'GnC_Cy
Cuts at: 2712 2875 3013 3250 3293 3294 3362 3394 3425
Size: 163 138 237 43 1 68 32 31
Cuts at: 3425 3433 2712
Size: 8 5664
Fragments arranged by size:
5664 237 163 138 68 43 32 31
8 1
EcoRI G'AATT_C
Cuts at: 3694 4072 3694
Size: 378 6007
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 6385
FspI TGC'GCA
Cuts at: 2912 3358 4891 2912
Size: 446 1533 4406
Fragments arranged by size:
4406 1533 446
HaeII r_GCGC'y
Cuts at: 1678 2239 2661 3006 3277 5532 1678
Size: 561 422 345 271 2255 2531
Fragments arranged by size:
2531 2255 561 422 345 271
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2589 2621 3449 3481 5026
Size: 32 135 32 1017 32 828 32 1545
Cuts at: 5026 5058 5100 5132 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1545 1017 828 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2443 2817 3729 3975 669
Size: 988 281 505 374 912 246 3079
Fragments arranged by size:
3079 988 912 505 374 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6385
HpaI GTT'AAC
Cuts at: 2443 3975 2443
Size: 1532 4853
KasI G'GCGC_C
Cuts at: 2657 3002 2657
Size: 345 6040
KpnI G_GTAC'C
Cuts at: 3710 3710
Size: 6385
MfeI C'AATT_G
Cuts at: 3984 3984
Size: 6385
MluI A'CGCG_T
Cuts at: 6261 6261
Size: 6385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2274 2508 3072 5499 5683 1305
Size: 969 234 564 2427 184 2007
Fragments arranged by size:
2427 2007 969 564 234 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3645 10
Size: 54 1425 343 1813 2750
Fragments arranged by size:
2750 1813 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4343 4702 4861 518
Size: 648 3177 359 159 2042
Fragments arranged by size:
3177 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2190 2217 2836 2908 2980 3199 3452 3801
Size: 584 27 619 72 72 219 253 349
Cuts at: 3801 4478 5626 5871 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 619 584 349 253 245
219 72 72 27
NaeI GCC'GGC
Cuts at: 3379 3379
Size: 6385
NarI GG'CG_CC
Cuts at: 2658 3003 2658
Size: 345 6040
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5833
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6385
NgoMIV G'CCGG_C
Cuts at: 3377 3377
Size: 6385
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6385
NotI GC'GGCC_GC
Cuts at: 3832 3832
Size: 6385
NspI r_CATG'y
Cuts at: 1132 1229 2428 3743 5288 1132
Size: 97 1199 1315 1545 2229
Fragments arranged by size:
2229 1545 1315 1199 97
PasI CC'CwG_GG
Cuts at: 1348 3365 3513 1348
Size: 2017 148 4220
Fragments arranged by size:
4220 2017 148
PciI A'CATG_T
Cuts at: 2424 5284 2424
Size: 2860 3525
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6385
PfoI T'CCnGG_A
Cuts at: 2091 2373 2091
Size: 282 6103
PmeI GTTT'AAAC
Cuts at: 3689 3689
Size: 6385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2660 2692 4454 4486 5967 5999 2660
Size: 32 1762 32 1481 32 3046
Fragments arranged by size:
3046 1762 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3250 3250
Size: 6385
PshAI GACnn'nnGTC
Cuts at: 1613 3248 1613
Size: 1635 4750
PsiI TTA'TAA
Cuts at: 3955 3955
Size: 6385
PspOMI G'GGCC_C
Cuts at: 3293 3393 3424 3433 3522 3293
Size: 100 31 9 89 6156
Fragments arranged by size:
6156 100 89 31 9
PstI C_TGCA'G
Cuts at: 830 2202 2235 2451 2676 2883 3737 830
Size: 1372 33 216 225 207 854 3478
Fragments arranged by size:
3478 1372 854 225 216 207 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6385
PvuII CAG'CTG
Cuts at: 2908 3199 2908
Size: 291 6094
SacI G_AGCT'C
Cuts at: 2457 2847 3027 2457
Size: 390 180 5815
Fragments arranged by size:
5815 390 180
SacII CC_GC'GG
Cuts at: 2191 2981 2191
Size: 790 5595
SalI G'TCGA_C
Cuts at: 1936 3727 1936
Size: 1791 4594
SapI GCTCTTCn'nnn_
Cuts at: 2180 2864 3336 2180
Size: 684 472 5229
Fragments arranged by size:
5229 684 472
SbfI CC_TGCA'GG
Cuts at: 2451 2883 3737 2451
Size: 432 854 5099
Fragments arranged by size:
5099 854 432
ScaI AGT'ACT
Cuts at: 4633 6345 4633
Size: 1712 4673
SfcI C'TryA_G
Cuts at: 826 1045 2198 2231 2447 2672 2879 3733 4868
Size: 219 1153 33 216 225 207 854 1135
Cuts at: 4868 5549 5740 826
Size: 681 191 1471
Fragments arranged by size:
1471 1153 1135 854 681 225 219 216
207 191 33
SfiI GGCCn_nnn'nGGCC
Cuts at: 3432 3432
Size: 6385
SfoI GGC'GCC
Cuts at: 2659 3004 2659
Size: 345 6040
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6385
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6385
SmaI CCC'GGG
Cuts at: 1872 2710 2850 3423 3431 3712 1872
Size: 838 140 573 8 281 4545
Fragments arranged by size:
4545 838 573 281 140 8
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2591 4489 5390 5652 820
Size: 197 926 648 1898 901 262 1553
Fragments arranged by size:
1898 1553 926 901 648 262 197
SnaBI TAC'GTA
Cuts at: 493 4176 493
Size: 3683 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6385
SphI G_CATG'C
Cuts at: 1132 3743 1132
Size: 2611 3774
SrfI GCCC'GGGC
Cuts at: 3423 3423
Size: 6385
SspI AAT'ATT
Cuts at: 5 52 4309 5
Size: 47 4257 2081
Fragments arranged by size:
4257 2081 47
StuI AGG'CCT
Cuts at: 1517 2145 3327 1517
Size: 628 1182 4575
Fragments arranged by size:
4575 1182 628
StyI C'CwwG_G
Cuts at: 513 1065 2562 3016 3322 3489 3679 3824 513
Size: 552 1497 454 306 167 190 145 3074
Fragments arranged by size:
3074 1497 552 454 306 190 167 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2918 3498 3505 4412 4429 4582 4767
Size: 1255 1338 580 7 907 17 153 185
Cuts at: 4767 5212 325
Size: 445 1498
Fragments arranged by size:
1498 1338 1255 907 580 445 185 153
17 7
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4631 6343 96
Size: 274 80 33 51 1443 2654 1712 138
Fragments arranged by size:
2654 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2326 2358 2681 2713 6345 6377 2326
Size: 32 323 32 3632 32 2334
Fragments arranged by size:
3632 2334 323 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6385
XbaI T'CTAG_A
Cuts at: 3721 3721
Size: 6385
XhoI C'TCGA_G
Cuts at: 1943 2591 1943
Size: 648 5737
XmaI C'CCGG_G
Cuts at: 1870 2708 2848 3421 3429 3710 1870
Size: 838 140 573 8 281 4545
Fragments arranged by size:
4545 838 573 281 140 8
XmnI GAAnn'nnTTC
Cuts at: 4514 4514
Size: 6385
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4193 276
Size: 53 83 186 936 2659 2468
Fragments arranged by size:
2659 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BfrBI BsiWI BsmBI BstZ17I DraIII
EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SanDI SexAI SwaI XcmI