Restriction map for
pFN21AE0069
(Circular) (Six-base) MAPSORT of: pFN21AE0069.seq Check: 6630 from: 1 to: 6919
pFN21AE0069 6919 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3522 4729 278
Size: 53 83 186 936 1986 1207 2468
Fragments arranged by size:
2468 1986 1207 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3249 4262 1937
Size: 1312 1013 4594
Fragments arranged by size:
4594 1312 1013
Acc65I G'GTAC_C
Cuts at: 2315 4240 2315
Size: 1925 4994
AclI AA'CG_TT
Cuts at: 1563 5046 5419 1563
Size: 3483 373 3063
Fragments arranged by size:
3483 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2297 3667 4982 6366 6634 1632
Size: 665 1370 1315 1384 268 1917
Fragments arranged by size:
1917 1384 1370 1315 665 268
AfeI AGC'GCT
Cuts at: 3425 3425
Size: 6919
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6722
AflIII A'CryG_T
Cuts at: 1203 5818 6795 1203
Size: 4615 977 1327
Fragments arranged by size:
4615 1327 977
AgeI A'CCGG_T
Cuts at: 5750 5750
Size: 6919
AhdI GACnn_n'nnGTC
Cuts at: 5648 5648
Size: 6919
AleI CACnn'nnGTG
Cuts at: 1166 3530 1166
Size: 2364 4555
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2087 1184
Size: 903 6016
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3572 3604 3572
Size: 32 6887
AlwNI CAG_nnn'CTG
Cuts at: 2746 2908 3946 4662 6234 2746
Size: 162 1038 716 1572 3431
Fragments arranged by size:
3431 1572 1038 716 162
ApaI G_GGCC'C
Cuts at: 3826 3826
Size: 6919
ApaLI G'TGCA_C
Cuts at: 3600 4976 6132 3600
Size: 1376 1156 4387
Fragments arranged by size:
4387 1376 1156
ApoI r'AATT_y
Cuts at: 1493 4228 4454 4606 6715 1493
Size: 2735 226 152 2109 1697
Fragments arranged by size:
2735 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5473 160
Size: 872 4441 1606
Fragments arranged by size:
4441 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6919
AvaI C'yCGr_G
Cuts at: 1870 1943 2524 2826 3125 3825 4132 4154 4244
Size: 73 581 302 299 700 307 22 90
Cuts at: 4244 1870
Size: 4545
Fragments arranged by size:
4545 700 581 307 302 299 90 73
22
BamHI G'GATC_C
Cuts at: 3231 4249 4616 3231
Size: 1018 367 5534
Fragments arranged by size:
5534 1018 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2174 2315 2649 2830 3476
Size: 325 217 627 387 141 334 181 646
Cuts at: 3476 3677 3687 4045 4150 4159 4240 5695 618
Size: 201 10 358 105 9 81 1455 1842
Fragments arranged by size:
1842 1455 646 627 387 358 334 325
217 201 181 141 105 81 10 9
BanII G_rGCy'C
Cuts at: 1391 2026 2354 2612 3771 3786 3826 4238 1391
Size: 635 328 258 1159 15 40 412 4072
Fragments arranged by size:
4072 1159 635 412 328 258 40 15
BbeI G_GCGC'C
Cuts at: 3681 4154 4163 3681
Size: 473 9 6437
Fragments arranged by size:
6437 473 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2726 3299 3345 928
Size: 971 827 573 46 4502
Fragments arranged by size:
4502 971 827 573 46
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2038 2072 5110 5144 1903
Size: 34 50 34 17 34 3038 34 3678
Fragments arranged by size:
3678 3038 50 34 34 34 34 17
BciVI GTATCCnnnnn_n'
Cuts at: 4811 6021 4811
Size: 1210 5709
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6919
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2216 2400 2922
Size: 107 122 71 631 605 544 184 522
Cuts at: 2922 3456 5530 136
Size: 534 2074 1525
Fragments arranged by size:
2074 1525 631 605 544 534 522 184
122 107 71
BglII A'GATC_T
Cuts at: 6914 6914
Size: 6919
BlpI GC'TnA_GC
Cuts at: 3240 3780 4336 3240
Size: 540 556 5823
Fragments arranged by size:
5823 556 540
Bme1580I G_kGCm'C
Cuts at: 1790 2177 2433 3006 3481 3564 3604 3815 3826
Size: 387 256 573 475 83 40 211 11
Cuts at: 3826 4980 6136 1790
Size: 1154 1156 2573
Fragments arranged by size:
2573 1156 1154 573 475 387 256 211
83 40 11
BmgBI CAC'GTC
Cuts at: 1318 2674 1318
Size: 1356 5563
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3835 4650 5608
Size: 416 53 83 399 24 2418 815 958
Cuts at: 5608 442
Size: 1753
Fragments arranged by size:
2418 1753 958 815 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6919
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2040 2040
Size: 6919
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2636 2723 5579 1239
Size: 1397 87 2856 2579
Fragments arranged by size:
2856 2579 1397 87
Bpu10I CC'TnA_GC
Cuts at: 3785 3785
Size: 6919
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3161 5044 5909 6207 3161
Size: 1883 865 298 3873
Fragments arranged by size:
3873 1883 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2531 5582 882
Size: 617 1032 3051 2219
Fragments arranged by size:
3051 2219 1032 617
BsaAI yAC'GTr
Cuts at: 493 1204 3361 4096 4710 493
Size: 711 2157 735 614 2702
Fragments arranged by size:
2702 2157 735 711 614
BsaBI GATnn'nnATC
Cuts at: 6913 6913
Size: 6919
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2223 3519 3678 4151
Size: 53 83 186 936 690 1296 159 473
Cuts at: 4151 4160 4726 5108 5743 275
Size: 9 566 382 635 1451
Fragments arranged by size:
1451 1296 936 690 635 566 473 382
186 159 83 53 9
BsaWI w'CCGG_w
Cuts at: 2646 5352 5750 6024 6171 6582 2646
Size: 2706 398 274 147 411 2983
Fragments arranged by size:
2983 2706 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2060 2090 3572 3602 1178
Size: 30 165 30 657 30 1482 30 4495
Fragments arranged by size:
4495 1482 657 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2150 2256 2362 3580 3982 1186
Size: 964 106 106 1218 402 4123
Fragments arranged by size:
4123 1218 964 402 106 106
BseYI C'CCAG_C
Cuts at: 3034 3459 4081 6122 6618 3034
Size: 425 622 2041 496 3335
Fragments arranged by size:
3335 2041 622 496 425
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3035 1163
Size: 573 1299 5047
Fragments arranged by size:
5047 1299 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2458 4369 5130 6158 1540
Size: 275 186 457 1911 761 1028 2301
Fragments arranged by size:
2301 1911 1028 761 457 275 186
BsiHKAI G_wGCw'C
Cuts at: 2058 2271 2354 3117 3327 3604 3786 4133 4980
Size: 213 83 763 210 277 182 347 847
Cuts at: 4980 5065 6136 2058
Size: 85 1071 2841
Fragments arranged by size:
2841 1071 847 763 347 277 213 210
182 85 83
BsiWI C'GTAC_G
Cuts at: 3297 3297
Size: 6919
BsmI GAATG_Cn'
Cuts at: 3706 4430 4523 3706
Size: 724 93 6102
Fragments arranged by size:
6102 724 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3370 3948 4125 3370
Size: 578 177 6164
Fragments arranged by size:
6164 578 177
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2026 2058 2177 2271 2354 2433 2612
Size: 399 236 32 119 94 83 79 179
Cuts at: 2612 3006 3117 3327 3481 3564 3604 3771 3786
Size: 394 111 210 154 83 40 167 15
Cuts at: 3786 3815 3826 4133 4238 4980 5065 6136 1391
Size: 29 11 307 105 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 394 307 236 210
179 167 154 119 111 105 94 85
83 83 79 40 32 29 15 11
BspEI T'CCGG_A
Cuts at: 6582 6582
Size: 6919
BspHI T'CATG_A
Cuts at: 4808 6538 4808
Size: 1730 5189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3057 3108 3235 4023 4274 844
Size: 2213 51 127 788 251 3489
Fragments arranged by size:
3489 2213 788 251 127 51
BsrBI CCG'CTC
Cuts at: 1394 1823 2019 2454 3229 3658 4806 1394
Size: 429 196 435 775 429 1148 3507
Fragments arranged by size:
3507 1148 775 435 429 429 196
BsrDI GCAATG_nn'
Cuts at: 65 1248 5414 5588 65
Size: 1183 4166 174 1396
Fragments arranged by size:
4166 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2281 3084 3165 3437 5563 5750 1700
Size: 110 471 803 81 272 2126 187 2869
Fragments arranged by size:
2869 2126 803 471 272 187 110 81
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6919
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6919
BssSI C'ACGA_G
Cuts at: 2918 4979 5991 2918
Size: 2061 1012 3846
Fragments arranged by size:
3846 2061 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6919
BstBI TT'CG_AA
Cuts at: 5723 5723
Size: 6919
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6919
BstXI CCAn_nnnn'nTGG
Cuts at: 2233 3140 2233
Size: 907 6012
BstYI r'GATC_y
Cuts at: 1972 2968 3129 3186 3231 4249 4616 5001 5018
Size: 996 161 57 45 1018 367 385 17
Cuts at: 5018 6470 6556 6568 6914 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1018 996 385 367 346 161
86 57 45 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2331 2433 2844 3153 3369 3471 3673
Size: 552 1266 102 411 309 216 102 202
Cuts at: 3673 4072 513
Size: 399 3360
Fragments arranged by size:
3360 1266 552 411 399 309 216 202
102 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2188 2493 2992 487
Size: 683 56 962 305 499 4414
Fragments arranged by size:
4414 962 683 499 305 56
BtsI GCAGTG_nn'
Cuts at: 2188 2819 3217 3537 4133 4435 5229 5249 2188
Size: 631 398 320 596 302 794 20 3858
Fragments arranged by size:
3858 794 631 596 398 320 302 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6919
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6884
DraI TTT'AAA
Cuts at: 4223 4223
Size: 6919
DraIII CAC_nnn'GTG
Cuts at: 2805 2845 3883 2805
Size: 40 1038 5841
Fragments arranged by size:
5841 1038 40
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2192 3518 5926 809
Size: 507 876 1326 2408 1802
Fragments arranged by size:
2408 1802 1326 876 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2455 2959 3150
Size: 54 1425 50 275 18 625 504 191
Cuts at: 3150 3366 3754 4006 4366 5255 6884 8
Size: 216 388 252 360 889 1629 43
Fragments arranged by size:
1629 1425 889 625 504 388 360 275
252 216 191 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2455 4366 1537
Size: 275 643 1911 4090
Fragments arranged by size:
4090 1911 643 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2981 4849 1353
Size: 1628 1868 3423
Fragments arranged by size:
3423 1868 1628
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2170 2672 3152 5506 5880 6026
Size: 1064 138 581 502 480 2354 374 146
Cuts at: 6026 387
Size: 1280
Fragments arranged by size:
2354 1280 1064 581 502 480 374 146
138
EcoICRI GAG'CTC
Cuts at: 2352 3784 2352
Size: 1432 5487
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2297 2636 2723 3667 4982 5579 6366
Size: 393 665 339 87 944 1315 597 787
Cuts at: 6366 6634 1239
Size: 268 1524
Fragments arranged by size:
1524 1315 944 787 665 597 393 339
268 87
EcoNI CCTnn'n_nnAGG
Cuts at: 3721 3721
Size: 6919
EcoO109I rG'GnC_Cy
Cuts at: 2640 2743 2872 3822 3943 2640
Size: 103 129 950 121 5616
Fragments arranged by size:
5616 950 129 121 103
EcoRI G'AATT_C
Cuts at: 4228 4606 4228
Size: 378 6541
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4034 4034
Size: 6919
FspI TGC'GCA
Cuts at: 2048 5425 2048
Size: 3377 3542
HaeII r_GCGC'y
Cuts at: 1678 3427 3681 4106 4154 4163 6066 1678
Size: 1749 254 425 48 9 1903 2531
Fragments arranged by size:
2531 1903 1749 425 254 48 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2232 2264 2975 3007 3483
Size: 32 135 32 660 32 711 32 476
Cuts at: 3483 3515 3773 3805 4105 4137 5560 5592 5634
Size: 32 258 32 300 32 1423 32 42
Cuts at: 5634 5666 1373
Size: 32 2626
Fragments arranged by size:
2626 1423 711 660 476 300 258 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3250 4263 4509 669
Size: 988 281 1312 1013 246 3079
Fragments arranged by size:
3079 1312 1013 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6919
HpaI GTT'AAC
Cuts at: 4509 4509
Size: 6919
KasI G'GCGC_C
Cuts at: 3677 4150 4159 3677
Size: 473 9 6437
Fragments arranged by size:
6437 473 9
KpnI G_GTAC'C
Cuts at: 2319 4244 2319
Size: 1925 4994
MfeI C'AATT_G
Cuts at: 4518 4518
Size: 6919
MluI A'CGCG_T
Cuts at: 6795 6795
Size: 6919
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4020 4033 6033 6217 1305
Size: 2715 13 2000 184 2007
Fragments arranged by size:
2715 2007 2000 184 13
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2961 4008 10
Size: 54 1425 343 1129 1047 2921
Fragments arranged by size:
2921 1425 1129 1047 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2069 3152 3530 4877 5236 5395 518
Size: 648 903 1083 378 1347 359 159 2042
Fragments arranged by size:
2042 1347 1083 903 648 378 359 159
MspA1I CmG'CkG
Cuts at: 1606 2517 2637 3172 3555 3675 4074 4142 4174
Size: 911 120 535 383 120 399 68 32
Cuts at: 4174 4335 5012 6160 6405 1606
Size: 161 677 1148 245 2120
Fragments arranged by size:
2120 1148 911 677 535 399 383 245
161 120 120 68 32
NaeI GCC'GGC
Cuts at: 3167 3167
Size: 6919
NarI GG'CG_CC
Cuts at: 3678 4151 4160 3678
Size: 473 9 6437
Fragments arranged by size:
6437 473 9
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6367
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6919
NgoMIV G'CCGG_C
Cuts at: 3165 3165
Size: 6919
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6919
NotI GC'GGCC_GC
Cuts at: 2455 4366 2455
Size: 1911 5008
NspI r_CATG'y
Cuts at: 1132 1229 4277 5822 1132
Size: 97 3048 1545 2229
Fragments arranged by size:
3048 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3771 1348
Size: 2423 4496
PciI A'CATG_T
Cuts at: 5818 5818
Size: 6919
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6919
PfoI T'CCnGG_A
Cuts at: 2133 2133
Size: 6919
PmeI GTTT'AAAC
Cuts at: 4223 4223
Size: 6919
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3572 3604 4988 5020 6501 6533 3572
Size: 32 1384 32 1481 32 3958
Fragments arranged by size:
3958 1481 1384 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3943 3943
Size: 6919
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6919
PsiI TTA'TAA
Cuts at: 4489 4489
Size: 6919
PspOMI G'GGCC_C
Cuts at: 3822 3822
Size: 6919
PstI C_TGCA'G
Cuts at: 830 2573 3457 4271 830
Size: 1743 884 814 3478
Fragments arranged by size:
3478 1743 884 814
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6919
PvuII CAG'CTG
Cuts at: 2517 3555 2517
Size: 1038 5881
SacI G_AGCT'C
Cuts at: 2354 3786 2354
Size: 1432 5487
SacII CC_GC'GG
Cuts at: 3676 4075 3676
Size: 399 6520
SalI G'TCGA_C
Cuts at: 1936 3248 4261 1936
Size: 1312 1013 4594
Fragments arranged by size:
4594 1312 1013
SbfI CC_TGCA'GG
Cuts at: 3457 4271 3457
Size: 814 6105
ScaI AGT'ACT
Cuts at: 5167 6879 5167
Size: 1712 5207
SexAI A'CCwGG_T
Cuts at: 2442 2442
Size: 6919
SfcI C'TryA_G
Cuts at: 826 1045 2569 2632 3453 4267 5402 6083 6274
Size: 219 1524 63 821 814 1135 681 191
Cuts at: 6274 826
Size: 1471
Fragments arranged by size:
1524 1471 1135 821 814 681 219 191
63
SfoI GGC'GCC
Cuts at: 3679 4152 4161 3679
Size: 473 9 6437
Fragments arranged by size:
6437 473 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6919
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6919
SmaI CCC'GGG
Cuts at: 1872 2526 2828 4156 4246 1872
Size: 654 302 1328 90 4545
Fragments arranged by size:
4545 1328 654 302 90
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3176 5023 5924 6186 820
Size: 197 926 1233 1847 901 262 1553
Fragments arranged by size:
1847 1553 1233 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4710 493
Size: 4217 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6919
SphI G_CATG'C
Cuts at: 1132 4277 1132
Size: 3145 3774
SspI AAT'ATT
Cuts at: 5 52 4843 5
Size: 47 4791 2081
Fragments arranged by size:
4791 2081 47
StuI AGG'CCT
Cuts at: 1517 2129 2954 1517
Size: 612 825 5482
Fragments arranged by size:
5482 825 612
StyI C'CwwG_G
Cuts at: 513 1065 3402 3507 4358 513
Size: 552 2337 105 851 3074
Fragments arranged by size:
3074 2337 851 552 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3464 4946 4963 5116 5301 5746 325
Size: 1255 1884 1482 17 153 185 445 1498
Fragments arranged by size:
1884 1498 1482 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2482 5165 6877
Size: 274 80 33 51 1443 505 2683 1712
Cuts at: 6877 96
Size: 138
Fragments arranged by size:
2683 1712 1443 505 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2060 2092 2582 2614 6879 6911 2060
Size: 32 490 32 4265 32 2068
Fragments arranged by size:
4265 2068 490 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6919
XbaI T'CTAG_A
Cuts at: 4255 4255
Size: 6919
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3947 3947
Size: 6919
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6919
XmaI C'CCGG_G
Cuts at: 1870 2524 2826 4154 4244 1870
Size: 654 302 1328 90 4545
Fragments arranged by size:
4545 1328 654 302 90
XmnI GAAnn'nnTTC
Cuts at: 3974 5048 3974
Size: 1074 5845
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3520 4727 276
Size: 53 83 186 936 1986 1207 2468
Fragments arranged by size:
2468 1986 1207 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BfrBI BstZ17I Bsu36I
EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SanDI SapI SfiI SrfI SwaI