Restriction map for
pFN21AE0065
(Circular) (Six-base) MAPSORT of: pFN21AE0065.seq Check: 1501 from: 1 to: 7795
pFN21AE0065 7795 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5605 278
Size: 53 83 186 936 4069 2468
Fragments arranged by size:
4069 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3179 5138 1937
Size: 1242 1959 4594
Fragments arranged by size:
4594 1959 1242
Acc65I G'GTAC_C
Cuts at: 5116 5116
Size: 7795
AclI AA'CG_TT
Cuts at: 1563 5922 6295 1563
Size: 4359 373 3063
Fragments arranged by size:
4359 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2858 3264 3531 5858 7242 7510 1632
Size: 1226 406 267 2327 1384 268 1917
Fragments arranged by size:
2327 1917 1384 1226 406 268 267
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7598
AflIII A'CryG_T
Cuts at: 1203 2583 5005 6694 7671 1203
Size: 1380 2422 1689 977 1327
Fragments arranged by size:
2422 1689 1380 1327 977
AgeI A'CCGG_T
Cuts at: 6626 6626
Size: 7795
AhdI GACnn_n'nnGTC
Cuts at: 2587 3490 6524 2587
Size: 903 3034 3858
Fragments arranged by size:
3858 3034 903
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7795
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7795
AlwNI CAG_nnn'CTG
Cuts at: 3049 5538 7110 3049
Size: 2489 1572 3734
Fragments arranged by size:
3734 2489 1572
ApaI G_GGCC'C
Cuts at: 3523 5038 3523
Size: 1515 6280
ApaLI G'TGCA_C
Cuts at: 5852 7008 5852
Size: 1156 6639
ApoI r'AATT_y
Cuts at: 1493 2039 2132 2796 2830 3577 4609 4623 5104
Size: 546 93 664 34 747 1032 14 481
Cuts at: 5104 5330 5482 7591 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1032 747 664 546 481 226
152 93 34 14
AseI AT'TA_AT
Cuts at: 160 1032 6349 160
Size: 872 5317 1606
Fragments arranged by size:
5317 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7795
AvaI C'yCGr_G
Cuts at: 1870 1943 3066 4472 5120 1870
Size: 73 1123 1406 648 4545
Fragments arranged by size:
4545 1406 1123 648 73
BamHI G'GATC_C
Cuts at: 5125 5492 5125
Size: 367 7428
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2379 4016 4467 5116 6571
Size: 325 217 627 592 1637 451 649 1455
Cuts at: 6571 618
Size: 1842
Fragments arranged by size:
1842 1637 1455 649 627 592 451 325
217
BanII G_rGCy'C
Cuts at: 1391 2745 3523 3537 4864 5038 5114 1391
Size: 1354 778 14 1327 174 76 4072
Fragments arranged by size:
4072 1354 1327 778 174 76 14
BbeI G_GCGC'C
Cuts at: 4471 4471
Size: 7795
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4744 928
Size: 971 2845 3979
Fragments arranged by size:
3979 2845 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3607 3641 3972 4006 5986 6020 1903
Size: 34 1670 34 331 34 1980 34 3678
Fragments arranged by size:
3678 1980 1670 331 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5687 6897 5687
Size: 1210 6585
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7795
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6406 136
Size: 107 122 71 631 605 4734 1525
Fragments arranged by size:
4734 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3023 3190 3320 3986 4479 4486 7790 3023
Size: 167 130 666 493 7 3304 3028
Fragments arranged by size:
3304 3028 666 493 167 130 7
BlpI GC'TnA_GC
Cuts at: 2615 3629 5212 2615
Size: 1014 1583 5198
Fragments arranged by size:
5198 1583 1014
Bme1580I G_kGCm'C
Cuts at: 1790 3478 3523 4931 5038 5856 7012 1790
Size: 1688 45 1408 107 818 1156 2573
Fragments arranged by size:
2573 1688 1408 1156 818 107 45
BmgBI CAC'GTC
Cuts at: 1318 3677 4169 1318
Size: 2359 492 4944
Fragments arranged by size:
4944 2359 492
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3421 3531 3800
Size: 416 53 83 399 24 2004 110 269
Cuts at: 3800 5526 6484 442
Size: 1726 958 1753
Fragments arranged by size:
2004 1753 1726 958 416 399 269 110
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4440 1056
Size: 3384 4411
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3977 4498 3977
Size: 521 7274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2661 3141 3951 3972 3991 4691 4864 4889
Size: 1422 480 810 21 19 700 173 25
Cuts at: 4889 4922 6455 1239
Size: 33 1533 2579
Fragments arranged by size:
2579 1533 1422 810 700 480 173 33
25 21 19
Bpu10I CC'TnA_GC
Cuts at: 4640 4640
Size: 7795
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2189 3290 3910 4301 5920 6785 7083 2189
Size: 1101 620 391 1619 865 298 2901
Fragments arranged by size:
2901 1619 1101 865 620 391 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3086 3995 6458 882
Size: 617 1587 909 2463 2219
Fragments arranged by size:
2463 2219 1587 909 617
BsaAI yAC'GTr
Cuts at: 493 1204 5586 493
Size: 711 4382 2702
Fragments arranged by size:
4382 2702 711
BsaBI GATnn'nnATC
Cuts at: 7789 7789
Size: 7795
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4468 5602 5984 6619
Size: 53 83 186 936 2935 1134 382 635
Cuts at: 6619 275
Size: 1451
Fragments arranged by size:
2935 1451 1134 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2745 6228 6626 6900 7047 7458 2745
Size: 3483 398 274 147 411 3082
Fragments arranged by size:
3483 3082 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4126 4156 1178
Size: 30 165 30 2723 30 4817
Fragments arranged by size:
4817 2723 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7795
BseYI C'CCAG_C
Cuts at: 2848 3368 3816 3945 4974 6998 7494 2848
Size: 520 448 129 1029 2024 496 3149
Fragments arranged by size:
3149 2024 1029 520 496 448 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3125 3747 1163
Size: 573 1389 622 5211
Fragments arranged by size:
5211 1389 622 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5245 6006 7034 1540
Size: 275 186 3244 761 1028 2301
Fragments arranged by size:
3244 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3732 5856 5941 7012 3732
Size: 2124 85 1071 4515
Fragments arranged by size:
4515 2124 1071 85
BsmI GAATG_Cn'
Cuts at: 3835 4268 5080 5306 5399 3835
Size: 433 812 226 93 6231
Fragments arranged by size:
6231 812 433 226 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2745 3478 3523 3537 3732 4864 4931
Size: 399 955 733 45 14 195 1132 67
Cuts at: 4931 5038 5114 5856 5941 7012 1391
Size: 107 76 742 85 1071 2174
Fragments arranged by size:
2174 1132 1071 955 742 733 399 195
107 85 76 67 45 14
BspEI T'CCGG_A
Cuts at: 7458 7458
Size: 7795
BspHI T'CATG_A
Cuts at: 4063 5684 7414 4063
Size: 1621 1730 4444
Fragments arranged by size:
4444 1730 1621
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5150 844
Size: 4306 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5682 1394
Size: 429 3859 3507
Fragments arranged by size:
3859 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6290 6464 65
Size: 1183 5042 174 1396
Fragments arranged by size:
5042 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2990 6439 6626 1700
Size: 110 1180 3449 187 2869
Fragments arranged by size:
3449 2869 1180 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7795
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7795
BssSI C'ACGA_G
Cuts at: 3616 5855 6867 3616
Size: 2239 1012 4544
Fragments arranged by size:
4544 2239 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7795
BstBI TT'CG_AA
Cuts at: 6599 6599
Size: 7795
BstEII G'GTnAC_C
Cuts at: 1184 4820 1184
Size: 3636 4159
BstXI CCAn_nnnn'nTGG
Cuts at: 3877 4464 4652 3877
Size: 587 188 7020
Fragments arranged by size:
7020 587 188
BstYI r'GATC_y
Cuts at: 1972 2046 3023 3190 3320 3986 4479 4486 4570
Size: 74 977 167 130 666 493 7 84
Cuts at: 4570 5125 5492 5877 5894 7346 7432 7444 7790
Size: 555 367 385 17 1452 86 12 346
Cuts at: 7790 1972
Size: 1977
Fragments arranged by size:
1977 1452 977 666 555 493 385 367
346 167 130 86 84 74 17 12
7
BtgI C'CryG_G
Cuts at: 513 1065 2577 2737 3469 4277 513
Size: 552 1512 160 732 808 4031
Fragments arranged by size:
4031 1512 808 732 552 160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2634 487
Size: 683 56 1408 5648
Fragments arranged by size:
5648 1408 683 56
BtsI GCAGTG_nn'
Cuts at: 3137 3374 4517 5311 6105 6125 3137
Size: 237 1143 794 794 20 4807
Fragments arranged by size:
4807 1143 794 794 237 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7795
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7760
DraI TTT'AAA
Cuts at: 5099 5099
Size: 7795
DraIII CAC_nnn'GTG
Cuts at: 3482 4845 3482
Size: 1363 6432
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6802 809
Size: 507 5486 1802
Fragments arranged by size:
5486 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5242 6131 7760
Size: 54 1425 50 275 18 3412 889 1629
Cuts at: 7760 8
Size: 43
Fragments arranged by size:
3412 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5242 1537
Size: 275 3430 4090
Fragments arranged by size:
4090 3430 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4027 4546 5725 1353
Size: 2674 519 1179 3423
Fragments arranged by size:
3423 2674 1179 519
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6382 6756 6902 387
Size: 1064 138 4793 374 146 1280
Fragments arranged by size:
4793 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2661 2858 3141 3264 3531 3951 3972
Size: 393 1029 197 283 123 267 420 21
Cuts at: 3972 3991 4691 4864 4889 4922 5858 6455 7242
Size: 19 700 173 25 33 936 597 787
Cuts at: 7242 7510 1239
Size: 268 1524
Fragments arranged by size:
1524 1029 936 787 700 597 420 393
283 268 267 197 173 123 33 25
21 19
EcoNI CCTnn'n_nnAGG
Cuts at: 3282 3448 4643 3282
Size: 166 1195 6434
Fragments arranged by size:
6434 1195 166
EcoO109I rG'GnC_Cy
Cuts at: 3519 5034 3519
Size: 1515 6280
EcoRI G'AATT_C
Cuts at: 3577 5104 5482 3577
Size: 1527 378 5890
Fragments arranged by size:
5890 1527 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2278 2964 3021 2278
Size: 686 57 7052
Fragments arranged by size:
7052 686 57
FspI TGC'GCA
Cuts at: 4523 6301 4523
Size: 1778 6017
HaeII r_GCGC'y
Cuts at: 1678 2562 4471 6942 1678
Size: 884 1909 2471 2531
Fragments arranged by size:
2531 2471 1909 884
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2573 2605 3476 3508 3978
Size: 32 135 32 1001 32 871 32 470
Cuts at: 3978 4010 4058 4090 4471 4503 6436 6468 6510
Size: 32 48 32 381 32 1933 32 42
Cuts at: 6510 6542 1373
Size: 32 2626
Fragments arranged by size:
2626 1933 1001 871 470 381 135 48
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2761 4203 5139 5385 669
Size: 988 281 823 1442 936 246 3079
Fragments arranged by size:
3079 1442 988 936 823 281 246
HindIII A'AGCT_T
Cuts at: 748 2711 3097 3886 748
Size: 1963 386 789 4657
Fragments arranged by size:
4657 1963 789 386
HpaI GTT'AAC
Cuts at: 2761 5385 2761
Size: 2624 5171
KasI G'GCGC_C
Cuts at: 4467 4467
Size: 7795
KpnI G_GTAC'C
Cuts at: 5120 5120
Size: 7795
MfeI C'AATT_G
Cuts at: 5394 5394
Size: 7795
MluI A'CGCG_T
Cuts at: 7671 7671
Size: 7795
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2225 2823 4416 6909 7093 1305
Size: 920 598 1593 2493 184 2007
Fragments arranged by size:
2493 2007 1593 920 598 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5973
Fragments arranged by size:
5973 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2388 2442 4077 4922 5073 5753 6112
Size: 648 1222 54 1635 845 151 680 359
Cuts at: 6112 6271 518
Size: 159 2042
Fragments arranged by size:
2042 1635 1222 845 680 648 359 159
151 54
MspA1I CmG'CkG
Cuts at: 1606 2756 2852 4711 4965 5211 5888 7036 7281
Size: 1150 96 1859 254 246 677 1148 245
Cuts at: 7281 1606
Size: 2120
Fragments arranged by size:
2120 1859 1150 1148 677 254 246 245
96
NaeI GCC'GGC
Cuts at: 2992 2992
Size: 7795
NarI GG'CG_CC
Cuts at: 4468 4468
Size: 7795
NcoI C'CATG_G
Cuts at: 513 1065 2737 3469 4277 513
Size: 552 1672 732 808 4031
Fragments arranged by size:
4031 1672 808 732 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7795
NgoMIV G'CCGG_C
Cuts at: 2990 2990
Size: 7795
NheI G'CTAG_C
Cuts at: 1052 4436 1052
Size: 3384 4411
NotI GC'GGCC_GC
Cuts at: 5242 5242
Size: 7795
NspI r_CATG'y
Cuts at: 1132 1229 2587 5153 6698 1132
Size: 97 1358 2566 1545 2229
Fragments arranged by size:
2566 2229 1545 1358 97
PasI CC'CwG_GG
Cuts at: 1348 3290 1348
Size: 1942 5853
PciI A'CATG_T
Cuts at: 2583 6694 2583
Size: 4111 3684
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2529 4721 1108
Size: 1421 2192 4182
Fragments arranged by size:
4182 2192 1421
PfoI T'CCnGG_A
Cuts at: 2638 2638
Size: 7795
PmeI GTTT'AAAC
Cuts at: 5099 5099
Size: 7795
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3323 3355 5864 5896 7377 7409 3323
Size: 32 2509 32 1481 32 3709
Fragments arranged by size:
3709 2509 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3078 3675 1613
Size: 1465 597 5733
Fragments arranged by size:
5733 1465 597
PsiI TTA'TAA
Cuts at: 5365 5365
Size: 7795
PspOMI G'GGCC_C
Cuts at: 3519 5034 3519
Size: 1515 6280
PstI C_TGCA'G
Cuts at: 830 3381 4494 5147 830
Size: 2551 1113 653 3478
Fragments arranged by size:
3478 2551 1113 653
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7795
PvuII CAG'CTG
Cuts at: 2756 2852 4711 4965 2756
Size: 96 1859 254 5586
Fragments arranged by size:
5586 1859 254 96
SalI G'TCGA_C
Cuts at: 1936 5137 1936
Size: 3201 4594
SbfI CC_TGCA'GG
Cuts at: 5147 5147
Size: 7795
ScaI AGT'ACT
Cuts at: 3001 4902 6043 7755 3001
Size: 1901 1141 1712 3041
Fragments arranged by size:
3041 1901 1712 1141
SfcI C'TryA_G
Cuts at: 826 1045 2719 3377 3448 4490 5143 6278 6959
Size: 219 1674 658 71 1042 653 1135 681
Cuts at: 6959 7150 826
Size: 191 1471
Fragments arranged by size:
1674 1471 1135 1042 681 658 653 219
191 71
SfoI GGC'GCC
Cuts at: 4469 4469
Size: 7795
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7795
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7795
SmaI CCC'GGG
Cuts at: 1872 3068 5122 1872
Size: 1196 2054 4545
Fragments arranged by size:
4545 2054 1196
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2204 3305 3889 4316 5899 6800
Size: 197 926 261 1101 584 427 1583 901
Cuts at: 6800 7062 820
Size: 262 1553
Fragments arranged by size:
1583 1553 1101 926 901 584 427 262
261 197
SnaBI TAC'GTA
Cuts at: 493 5586 493
Size: 5093 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7795
SphI G_CATG'C
Cuts at: 1132 5153 1132
Size: 4021 3774
SspI AAT'ATT
Cuts at: 5 52 3265 4297 5719 5
Size: 47 3213 1032 1422 2081
Fragments arranged by size:
3213 2081 1422 1032 47
StuI AGG'CCT
Cuts at: 1517 3060 4649 1517
Size: 1543 1589 4663
Fragments arranged by size:
4663 1589 1543
StyI C'CwwG_G
Cuts at: 513 1065 2050 2737 2775 2791 3456 3469 3870
Size: 552 985 687 38 16 665 13 401
Cuts at: 3870 4130 4277 5234 513
Size: 260 147 957 3074
Fragments arranged by size:
3074 985 957 687 665 552 401 260
147 38 16 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2526 3982 5822 5839 5992 6177 6622
Size: 1255 946 1456 1840 17 153 185 445
Cuts at: 6622 325
Size: 1498
Fragments arranged by size:
1840 1498 1456 1255 946 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2083 2259 2999
Size: 274 80 33 51 1443 106 176 740
Cuts at: 2999 4900 6041 7753 96
Size: 1901 1141 1712 138
Fragments arranged by size:
1901 1712 1443 1141 740 274 176 138
106 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2562 2594 4547 4579 4926 4958 7755 7787 2562
Size: 32 1953 32 347 32 2797 32 2570
Fragments arranged by size:
2797 2570 1953 347 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7795
XbaI T'CTAG_A
Cuts at: 5131 5131
Size: 7795
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7795
XmaI C'CCGG_G
Cuts at: 1870 3066 5120 1870
Size: 1196 2054 4545
Fragments arranged by size:
4545 2054 1196
XmnI GAAnn'nnTTC
Cuts at: 2238 2714 5924 2238
Size: 476 3210 4109
Fragments arranged by size:
4109 3210 476
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5603 276
Size: 53 83 186 936 4069 2468
Fragments arranged by size:
4069 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI