Restriction map for
pFN21AE0061
(Circular) (Six-base) MAPSORT of: pFN21AE0061.seq Check: 3093 from: 1 to: 7516
pFN21AE0061 7516 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3649 3649
Size: 7516
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5326 278
Size: 53 83 186 936 3790 2468
Fragments arranged by size:
3790 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2744 3383 4859 1937
Size: 807 639 1476 4594
Fragments arranged by size:
4594 1476 807 639
Acc65I G'GTAC_C
Cuts at: 3101 4837 3101
Size: 1736 5780
AclI AA'CG_TT
Cuts at: 1563 2766 5643 6016 1563
Size: 1203 2877 373 3063
Fragments arranged by size:
3063 2877 1203 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4303 5579 6963 7231 1632
Size: 2671 1276 1384 268 1917
Fragments arranged by size:
2671 1917 1384 1276 268
AfeI AGC'GCT
Cuts at: 3238 3238
Size: 7516
AflII C'TTAA_G
Cuts at: 820 1017 2896 820
Size: 197 1879 5440
Fragments arranged by size:
5440 1879 197
AflIII A'CryG_T
Cuts at: 1203 3525 3816 4095 6415 7392 1203
Size: 2322 291 279 2320 977 1327
Fragments arranged by size:
2322 2320 1327 977 291 279
AgeI A'CCGG_T
Cuts at: 3841 6347 3841
Size: 2506 5010
AhdI GACnn_n'nnGTC
Cuts at: 6245 6245
Size: 7516
AleI CACnn'nnGTG
Cuts at: 1166 4220 1166
Size: 3054 4462
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7516
AlwNI CAG_nnn'CTG
Cuts at: 2158 5259 6831 2158
Size: 3101 1572 2843
Fragments arranged by size:
3101 2843 1572
ApaI G_GGCC'C
Cuts at: 4295 4295
Size: 7516
ApaLI G'TGCA_C
Cuts at: 3533 4424 5573 6729 3533
Size: 891 1149 1156 4320
Fragments arranged by size:
4320 1156 1149 891
ApoI r'AATT_y
Cuts at: 1493 2210 2263 4625 4825 5051 5203 7312 1493
Size: 717 53 2362 200 226 152 2109 1697
Fragments arranged by size:
2362 2109 1697 717 226 200 152 53
AseI AT'TA_AT
Cuts at: 160 1032 6070 160
Size: 872 5038 1606
Fragments arranged by size:
5038 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7516
AvaI C'yCGr_G
Cuts at: 1870 1943 2111 2663 3377 3708 4841 1870
Size: 73 168 552 714 331 1133 4545
Fragments arranged by size:
4545 1133 714 552 331 168 73
BamHI G'GATC_C
Cuts at: 4846 5213 4846
Size: 367 7149
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2182 2675 3101 3703 4027
Size: 325 217 627 395 493 426 602 324
Cuts at: 4027 4837 6292 618
Size: 810 1455 1842
Fragments arranged by size:
1842 1455 810 627 602 493 426 395
325 324 217
BanII G_rGCy'C
Cuts at: 1391 2525 3299 3913 3975 4295 4835 1391
Size: 1134 774 614 62 320 540 4072
Fragments arranged by size:
4072 1134 774 614 540 320 62
BbeI G_GCGC'C
Cuts at: 3707 3707
Size: 7516
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6545
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5707 5741 1903
Size: 34 3770 34 3678
Fragments arranged by size:
3770 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2015 4501 5408 6618 2015
Size: 2486 907 1210 2913
Fragments arranged by size:
2913 2486 1210 907
BclI T'GATC_A
Cuts at: 1548 4104 4471 1548
Size: 2556 367 4593
Fragments arranged by size:
4593 2556 367
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6127 136
Size: 107 122 71 631 605 4455 1525
Fragments arranged by size:
4455 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7511 7511
Size: 7516
BlpI GC'TnA_GC
Cuts at: 4174 4933 4174
Size: 759 6757
Bme1580I G_kGCm'C
Cuts at: 1790 2015 3537 4295 4428 5577 6733 1790
Size: 225 1522 758 133 1149 1156 2573
Fragments arranged by size:
2573 1522 1156 1149 758 225 133
BmgBI CAC'GTC
Cuts at: 1318 2365 2548 3346 1318
Size: 1047 183 798 5488
Fragments arranged by size:
5488 1047 798 183
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2717 2954 5247
Size: 416 53 83 399 24 1300 237 2293
Cuts at: 5247 6205 442
Size: 958 1753
Fragments arranged by size:
2293 1753 1300 958 416 399 237 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3324 1056
Size: 2268 5248
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2111 2111
Size: 7516
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2186 2249 2342 2833 2847 3608 4213 4518
Size: 947 63 93 491 14 761 605 305
Cuts at: 4518 6176 1239
Size: 1658 2579
Fragments arranged by size:
2579 1658 947 761 605 491 305 93
63 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5641 6506 6804 5641
Size: 865 298 6353
Fragments arranged by size:
6353 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4067 4721 6179 882
Size: 617 2568 654 1458 2219
Fragments arranged by size:
2568 2219 1458 654 617
BsaAI yAC'GTr
Cuts at: 493 1204 3526 3788 5307 493
Size: 711 2322 262 1519 2702
Fragments arranged by size:
2702 2322 1519 711 262
BsaBI GATnn'nnATC
Cuts at: 7510 7510
Size: 7516
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3090 3173 3704 4037
Size: 53 83 186 936 1557 83 531 333
Cuts at: 4037 5323 5705 6340 275
Size: 1286 382 635 1451
Fragments arranged by size:
1557 1451 1286 936 635 531 382 333
186 83 83 53
BsaWI w'CCGG_w
Cuts at: 2395 2620 3841 4601 5949 6347 6621 6768 7179
Size: 225 1221 760 1348 398 274 147 411
Cuts at: 7179 2395
Size: 2732
Fragments arranged by size:
2732 1348 1221 760 411 398 274 225
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2831 2861 4379 4409 1178
Size: 30 165 30 1428 30 1518 30 4285
Fragments arranged by size:
4285 1518 1428 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 2297 3596 4448 4739 1186
Size: 943 168 1299 852 291 3963
Fragments arranged by size:
3963 1299 943 852 291 168
BseYI C'CCAG_C
Cuts at: 2404 4257 4325 6719 7215 2404
Size: 1853 68 2394 496 2705
Fragments arranged by size:
2705 2394 1853 496 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2329 3810 1163
Size: 573 593 1481 4869
Fragments arranged by size:
4869 1481 593 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2278 3852 4966 5727 6755 1540
Size: 275 186 277 1574 1114 761 1028 2301
Fragments arranged by size:
2301 1574 1114 1028 761 277 275 186
BsiHKAI G_wGCw'C
Cuts at: 2950 3537 4175 4229 4283 4428 5577 5662 6733
Size: 587 638 54 54 145 1149 85 1071
Cuts at: 6733 2950
Size: 3733
Fragments arranged by size:
3733 1149 1071 638 587 145 85 54
54
BsmI GAATG_Cn'
Cuts at: 4443 5027 5120 4443
Size: 584 93 6839
Fragments arranged by size:
6839 584 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3914 3914
Size: 7516
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2525 2950 3299 3537 3913 3975
Size: 399 225 510 425 349 238 376 62
Cuts at: 3975 4175 4229 4283 4295 4428 4835 5577 5662
Size: 200 54 54 12 133 407 742 85
Cuts at: 5662 6733 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 510 425 407 399 376
349 238 225 200 133 85 62 54
54 12
BspEI T'CCGG_A
Cuts at: 2620 7179 2620
Size: 4559 2957
BspHI T'CATG_A
Cuts at: 5405 7135 5405
Size: 1730 5786
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2338 2557 3170 3649 4871 844
Size: 1494 219 613 479 1222 3489
Fragments arranged by size:
3489 1494 1222 613 479 219
BsrBI CCG'CTC
Cuts at: 1394 1823 3546 5403 1394
Size: 429 1723 1857 3507
Fragments arranged by size:
3507 1857 1723 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4059 6011 6185 65
Size: 1183 2811 1952 174 1396
Fragments arranged by size:
2811 1952 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3691 3841 3851 6160 6347 1700
Size: 110 1881 150 10 2309 187 2869
Fragments arranged by size:
2869 2309 1881 187 150 110 10
BsrGI T'GTAC_A
Cuts at: 96 3005 3882 4155 96
Size: 2909 877 273 3457
Fragments arranged by size:
3457 2909 877 273
BssHII G'CGCG_C
Cuts at: 1925 3508 3510 3612 3614 1925
Size: 1583 2 102 2 5827
Fragments arranged by size:
5827 1583 102 2 2
BssSI C'ACGA_G
Cuts at: 3578 3906 5576 6588 3578
Size: 328 1670 1012 4506
Fragments arranged by size:
4506 1670 1012 328
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7516
BstBI TT'CG_AA
Cuts at: 6320 6320
Size: 7516
BstEII G'GTnAC_C
Cuts at: 1184 3157 3277 1184
Size: 1973 120 5423
Fragments arranged by size:
5423 1973 120
BstXI CCAn_nnnn'nTGG
Cuts at: 4000 4135 4237 4000
Size: 135 102 7279
Fragments arranged by size:
7279 135 102
BstYI r'GATC_y
Cuts at: 1972 2221 2955 4640 4846 5213 5598 5615 7067
Size: 249 734 1685 206 367 385 17 1452
Cuts at: 7067 7153 7165 7511 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1685 1452 734 385 367 346 249
206 86 17 12
BstZ17I GTA'TAC
Cuts at: 2745 3384 2745
Size: 639 6877
Bsu36I CC'TnA_GG
Cuts at: 4335 4335
Size: 7516
BtgI C'CryG_G
Cuts at: 513 1065 2004 2215 3330 3749 3964 513
Size: 552 939 211 1115 419 215 4065
Fragments arranged by size:
4065 1115 939 552 419 215 211
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2131 2413 3633 4093 487
Size: 683 56 905 282 1220 460 3910
Fragments arranged by size:
3910 1220 905 683 460 282 56
BtsI GCAGTG_nn'
Cuts at: 3315 3695 3738 4484 5032 5826 5846 3315
Size: 380 43 746 548 794 20 4985
Fragments arranged by size:
4985 794 746 548 380 43 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7516
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2197 2232 529
Size: 35 1633 35 5813
Fragments arranged by size:
5813 1633 35 35
DraI TTT'AAA
Cuts at: 4573 4820 4573
Size: 247 7269
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6523 809
Size: 507 5207 1802
Fragments arranged by size:
5207 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2643 3339 3849
Size: 54 1425 50 275 18 813 696 510
Cuts at: 3849 4021 4481 4963 5852 7481 8
Size: 172 460 482 889 1629 43
Fragments arranged by size:
1629 1425 889 813 696 510 482 460
275 172 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3849 4963 1537
Size: 275 2037 1114 4090
Fragments arranged by size:
4090 2037 1114 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5446 1353
Size: 4093 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2189 2311 3250 6103 6477 6623
Size: 1064 138 600 122 939 2853 374 146
Cuts at: 6623 387
Size: 1280
Fragments arranged by size:
2853 1280 1064 939 600 374 146 138
122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2249 2342 2833 2847 3608 4213
Size: 393 554 63 93 491 14 761 605
Cuts at: 4213 4303 4518 5579 6176 6963 7231 1239
Size: 90 215 1061 597 787 268 1524
Fragments arranged by size:
1524 1061 787 761 605 597 554 491
393 268 215 93 90 63 14
EcoNI CCTnn'n_nnAGG
Cuts at: 4334 4433 4334
Size: 99 7417
EcoO109I rG'GnC_Cy
Cuts at: 2155 4291 4466 2155
Size: 2136 175 5205
Fragments arranged by size:
5205 2136 175
EcoRI G'AATT_C
Cuts at: 2263 4825 5203 2263
Size: 2562 378 4576
Fragments arranged by size:
4576 2562 378
EcoRV GAT'ATC
Cuts at: 2488 2488
Size: 7516
FspI TGC'GCA
Cuts at: 6022 6022
Size: 7516
HaeII r_GCGC'y
Cuts at: 1678 3240 3707 4181 6663 1678
Size: 1562 467 474 2482 2531
Fragments arranged by size:
2531 2482 1562 474 467
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2459 2491 2858 2890 3242
Size: 32 135 32 887 32 367 32 352
Cuts at: 3242 3274 3281 3313 3788 3820 6157 6189 6231
Size: 32 7 32 475 32 2337 32 42
Cuts at: 6231 6263 1373
Size: 32 2626
Fragments arranged by size:
2626 2337 887 475 367 352 135 42
32 32 32 32 32 32 32 32
32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3054 3148 3796 4093 4552 4860
Size: 988 281 1116 94 648 297 459 308
Cuts at: 4860 5106 669
Size: 246 3079
Fragments arranged by size:
3079 1116 988 648 459 308 297 281
246 94
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7516
HpaI GTT'AAC
Cuts at: 5106 5106
Size: 7516
KasI G'GCGC_C
Cuts at: 3703 3703
Size: 7516
KpnI G_GTAC'C
Cuts at: 3105 4841 3105
Size: 1736 5780
MfeI C'AATT_G
Cuts at: 5115 5115
Size: 7516
MluI A'CGCG_T
Cuts at: 3816 7392 3816
Size: 3576 3940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3552 6630 6814 1305
Size: 2247 3078 184 2007
Fragments arranged by size:
3078 2247 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4483 10
Size: 54 1425 343 2651 3043
Fragments arranged by size:
3043 2651 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2796 4220 5474 5833 5992 518
Size: 648 1630 1424 1254 359 159 2042
Fragments arranged by size:
2042 1630 1424 1254 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2344 3751 4563 4932 5609 6757 7002 1606
Size: 738 1407 812 369 677 1148 245 2120
Fragments arranged by size:
2120 1407 1148 812 738 677 369 245
NaeI GCC'GGC
Cuts at: 3693 3693
Size: 7516
NarI GG'CG_CC
Cuts at: 3704 3704
Size: 7516
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6025
Fragments arranged by size:
6025 939 552
NdeI CA'TA_TG
Cuts at: 387 2925 387
Size: 2538 4978
NgoMIV G'CCGG_C
Cuts at: 3691 3691
Size: 7516
NheI G'CTAG_C
Cuts at: 1052 3320 1052
Size: 2268 5248
NotI GC'GGCC_GC
Cuts at: 4963 4963
Size: 7516
NspI r_CATG'y
Cuts at: 1132 1229 4200 4414 4874 6419 1132
Size: 97 2971 214 460 1545 2229
Fragments arranged by size:
2971 2229 1545 460 214 97
PasI CC'CwG_GG
Cuts at: 1348 2030 1348
Size: 682 6834
PciI A'CATG_T
Cuts at: 6415 6415
Size: 7516
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3775 1108
Size: 2667 4849
PfoI T'CCnGG_A
Cuts at: 2541 4459 2541
Size: 1918 5598
PmeI GTTT'AAAC
Cuts at: 4820 4820
Size: 7516
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5585 5617 7098 7130 5585
Size: 32 1481 32 5971
Fragments arranged by size:
5971 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2155 2155
Size: 7516
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7516
PsiI TTA'TAA
Cuts at: 2060 5086 2060
Size: 3026 4490
PspOMI G'GGCC_C
Cuts at: 4291 4291
Size: 7516
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2728 2760 3374 3406 2728
Size: 32 614 32 6838
Fragments arranged by size:
6838 614 32 32
PstI C_TGCA'G
Cuts at: 830 2244 2349 3567 3660 4868 830
Size: 1414 105 1218 93 1208 3478
Fragments arranged by size:
3478 1414 1218 1208 105 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7516
PvuII CAG'CTG
Cuts at: 4563 4563
Size: 7516
SacII CC_GC'GG
Cuts at: 3752 3752
Size: 7516
SalI G'TCGA_C
Cuts at: 1936 4858 1936
Size: 2922 4594
SbfI CC_TGCA'GG
Cuts at: 4868 4868
Size: 7516
ScaI AGT'ACT
Cuts at: 5764 7476 5764
Size: 1712 5804
SexAI A'CCwGG_T
Cuts at: 3097 4812 3097
Size: 1715 5801
SfcI C'TryA_G
Cuts at: 826 1045 2240 2345 3563 3656 4350 4864 5999
Size: 219 1195 105 1218 93 694 514 1135
Cuts at: 5999 6680 6871 826
Size: 681 191 1471
Fragments arranged by size:
1471 1218 1195 1135 694 681 514 219
191 105 93
SfoI GGC'GCC
Cuts at: 3705 3705
Size: 7516
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7516
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7516
SmaI CCC'GGG
Cuts at: 1872 4843 1872
Size: 2971 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2896 5620 6521 6783 820
Size: 197 926 953 2724 901 262 1553
Fragments arranged by size:
2724 1553 953 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5307 493
Size: 4814 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7516
SphI G_CATG'C
Cuts at: 1132 4414 4874 1132
Size: 3282 460 3774
Fragments arranged by size:
3774 3282 460
SspI AAT'ATT
Cuts at: 5 52 5440 5
Size: 47 5388 2081
Fragments arranged by size:
5388 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7516
StyI C'CwwG_G
Cuts at: 513 1065 2004 4955 513
Size: 552 939 2951 3074
Fragments arranged by size:
3074 2951 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2095 3147 3704 4091 4312 5543 5560
Size: 1255 515 1052 557 387 221 1231 17
Cuts at: 5560 5713 5898 6343 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1231 1052 557 515 445 387
221 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3005 3882 4155
Size: 274 80 33 51 1443 1028 877 273
Cuts at: 4155 5762 7474 96
Size: 1607 1712 138
Fragments arranged by size:
1712 1607 1443 1028 877 274 273 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2769 2801 2940 2972 7476 7508 2769
Size: 32 139 32 4504 32 2777
Fragments arranged by size:
4504 2777 139 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7516
XbaI T'CTAG_A
Cuts at: 4852 4852
Size: 7516
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2884 2884
Size: 7516
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7516
XmaI C'CCGG_G
Cuts at: 1870 4841 1870
Size: 2971 4545
XmnI GAAnn'nnTTC
Cuts at: 2423 5645 2423
Size: 3222 4294
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5324 276
Size: 53 83 186 936 3790 2468
Fragments arranged by size:
3790 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
DraIII EcoICRI FalI FseI FspAI NruI NsiI PacI
PmlI PspXI RsrII SacI SanDI SapI SfiI SrfI
SwaI