Restriction map for
pFN21AE0057
(Circular) (Six-base) MAPSORT of: pFN21AE0057.seq Check: 8775 from: 1 to: 8242
pFN21AE0057 8242 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3082 5193 3082
Size: 2111 6131
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6052 278
Size: 53 83 186 936 4516 2468
Fragments arranged by size:
4516 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5585 1937
Size: 3648 4594
Acc65I G'GTAC_C
Cuts at: 5563 5563
Size: 8242
AclI AA'CG_TT
Cuts at: 1563 6369 6742 1563
Size: 4806 373 3063
Fragments arranged by size:
4806 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2033 2098 2403 2937 3046 3431 3464 3776
Size: 401 65 305 534 109 385 33 312
Cuts at: 3776 3836 3944 5162 5458 5525 6305 7689 7957
Size: 60 108 1218 296 67 780 1384 268
Cuts at: 7957 1632
Size: 1917
Fragments arranged by size:
1917 1384 1218 780 534 401 385 312
305 296 268 109 108 67 65 60
33
AfeI AGC'GCT
Cuts at: 3767 3833 3767
Size: 66 8176
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8045
AflIII A'CryG_T
Cuts at: 1203 7141 8118 1203
Size: 5938 977 1327
Fragments arranged by size:
5938 1327 977
AgeI A'CCGG_T
Cuts at: 7073 7073
Size: 8242
AhdI GACnn_n'nnGTC
Cuts at: 6971 6971
Size: 8242
AleI CACnn'nnGTG
Cuts at: 1166 2571 1166
Size: 1405 6837
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2660 5454 1184
Size: 1476 2794 3972
Fragments arranged by size:
3972 2794 1476
AlwNI CAG_nnn'CTG
Cuts at: 3287 3409 4056 4144 4217 4305 5185 5329 5985
Size: 122 647 88 73 88 880 144 656
Cuts at: 5985 7557 3287
Size: 1572 3972
Fragments arranged by size:
3972 1572 880 656 647 144 122 88
88 73
ApaI G_GGCC'C
Cuts at: 4611 4611
Size: 8242
ApaLI G'TGCA_C
Cuts at: 6299 7455 6299
Size: 1156 7086
ApoI r'AATT_y
Cuts at: 1493 3290 3443 3977 5551 5777 5929 8038 1493
Size: 1797 153 534 1574 226 152 2109 1697
Fragments arranged by size:
2109 1797 1697 1574 534 226 153 152
AseI AT'TA_AT
Cuts at: 160 1032 6796 160
Size: 872 5764 1606
Fragments arranged by size:
5764 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8242
AvaI C'yCGr_G
Cuts at: 1870 1943 2943 3983 5567 1870
Size: 73 1000 1040 1584 4545
Fragments arranged by size:
4545 1584 1040 1000 73
BamHI G'GATC_C
Cuts at: 2486 4457 5572 5939 2486
Size: 1971 1115 367 4789
Fragments arranged by size:
4789 1971 1115 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2937 3094 3235 4540 4600
Size: 325 217 627 1150 157 141 1305 60
Cuts at: 4600 4916 5563 7018 618
Size: 316 647 1455 1842
Fragments arranged by size:
1842 1455 1305 1150 647 627 325 316
217 157 141 60
BanII G_rGCy'C
Cuts at: 1391 2051 2383 3990 4211 4611 4640 5391 5561
Size: 660 332 1607 221 400 29 751 170
Cuts at: 5561 1391
Size: 4072
Fragments arranged by size:
4072 1607 751 660 400 332 221 170
29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2316 2550 3166 3253 928
Size: 971 417 234 616 87 5917
Fragments arranged by size:
5917 971 616 417 234 87
BbvCI CC'TCA_GC
Cuts at: 3534 3625 3534
Size: 91 8151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6433 6467 1903
Size: 34 4496 34 3678
Fragments arranged by size:
4496 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3455 6134 7344 3455
Size: 2679 1210 4353
Fragments arranged by size:
4353 2679 1210
BclI T'GATC_A
Cuts at: 1548 2629 1548
Size: 1081 7161
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5456 6853 136
Size: 107 122 71 631 605 3784 1397 1525
Fragments arranged by size:
3784 1525 1397 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2856 3048 3808 8237 2856
Size: 192 760 4429 2861
Fragments arranged by size:
4429 2861 760 192
BlpI GC'TnA_GC
Cuts at: 2823 3309 4311 5659 2823
Size: 486 1002 1348 5406
Fragments arranged by size:
5406 1348 1002 486
Bme1580I G_kGCm'C
Cuts at: 1790 2942 4036 4603 4611 4921 6303 7459 1790
Size: 1152 1094 567 8 310 1382 1156 2573
Fragments arranged by size:
2573 1382 1156 1152 1094 567 310 8
BmgBI CAC'GTC
Cuts at: 1318 3514 1318
Size: 2196 6046
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2565 2680 3740
Size: 416 53 83 399 24 1148 115 1060
Cuts at: 3740 5973 6931 442
Size: 2233 958 1753
Fragments arranged by size:
2233 1753 1148 1060 958 416 399 115
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8242
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3448 4439 5157 3448
Size: 991 718 6533
Fragments arranged by size:
6533 991 718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2490 2792 3418 3952 5000 5052 5110 5351
Size: 1251 302 626 534 1048 52 58 241
Cuts at: 5351 6902 1239
Size: 1551 2579
Fragments arranged by size:
2579 1551 1251 1048 626 534 302 241
58 52
Bpu10I CC'TnA_GC
Cuts at: 2653 2844 3534 3625 3704 4069 5088 2653
Size: 191 690 91 79 365 1019 5807
Fragments arranged by size:
5807 1019 690 365 191 91 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2042 2221 4338 4959 6367 7232 7530 2042
Size: 179 2117 621 1408 865 298 2754
Fragments arranged by size:
2754 2117 1408 865 621 298 179
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4825 4861 6905 882
Size: 617 3326 36 2044 2219
Fragments arranged by size:
3326 2219 2044 617 36
BsaAI yAC'GTr
Cuts at: 493 1204 6033 493
Size: 711 4829 2702
Fragments arranged by size:
4829 2702 711
BsaBI GATnn'nnATC
Cuts at: 3677 4456 8236 3677
Size: 779 3780 3683
Fragments arranged by size:
3780 3683 779
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6049 6431 7066 275
Size: 53 83 186 936 4516 382 635 1451
Fragments arranged by size:
4516 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6675 7073 7347 7494 7905 6675
Size: 398 274 147 411 7012
Fragments arranged by size:
7012 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3035 3065 4474 4504 1178
Size: 30 165 30 1632 30 1409 30 4916
Fragments arranged by size:
4916 1632 1409 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2199 2743 3043 3776 3853 4950 1186
Size: 1013 544 300 733 77 1097 4478
Fragments arranged by size:
4478 1097 1013 733 544 300 77
BseYI C'CCAG_C
Cuts at: 2050 3015 3215 3797 4000 4098 4461 4867 4996
Size: 965 200 582 203 98 363 406 129
Cuts at: 4996 5050 5322 5384 7445 7941 2050
Size: 54 272 62 2061 496 2351
Fragments arranged by size:
2351 2061 965 582 496 406 363 272
203 200 129 98 62 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2951 1163
Size: 573 1215 6454
Fragments arranged by size:
6454 1215 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3741 4849 5692 6453 7481 1540
Size: 275 186 1740 1108 843 761 1028 2301
Fragments arranged by size:
2301 1740 1108 1028 843 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2702 3990 4070 4312 6303 6388 7459 2702
Size: 1288 80 242 1991 85 1071 3485
Fragments arranged by size:
3485 1991 1288 1071 242 85 80
BsmI GAATG_Cn'
Cuts at: 5753 5846 5753
Size: 93 8149
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2383 2702 2942 3990 4036 4070
Size: 399 261 332 319 240 1048 46 34
Cuts at: 4070 4211 4312 4603 4611 4640 4921 5391 5561
Size: 141 101 291 8 29 281 470 170
Cuts at: 5561 6303 6388 7459 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1048 742 470 399 332 319
291 281 261 240 170 141 101 85
46 34 29 8
BspEI T'CCGG_A
Cuts at: 7905 7905
Size: 8242
BspHI T'CATG_A
Cuts at: 6131 7861 6131
Size: 1730 6512
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2196 3082 5193 5597 844
Size: 1352 886 2111 404 3489
Fragments arranged by size:
3489 2111 1352 886 404
BsrBI CCG'CTC
Cuts at: 1394 1823 2026 4084 6129 1394
Size: 429 203 2058 2045 3507
Fragments arranged by size:
3507 2058 2045 429 203
BsrDI GCAATG_nn'
Cuts at: 65 1248 6737 6911 65
Size: 1183 5489 174 1396
Fragments arranged by size:
5489 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2535 6886 7073 1700
Size: 110 725 4351 187 2869
Fragments arranged by size:
4351 2869 725 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8242
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8242
BssSI C'ACGA_G
Cuts at: 6302 7314 6302
Size: 1012 7230
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2919 4690 5447 794
Size: 2125 1771 757 3589
Fragments arranged by size:
3589 2125 1771 757
BstBI TT'CG_AA
Cuts at: 7046 7046
Size: 8242
BstEII G'GTnAC_C
Cuts at: 1184 3415 1184
Size: 2231 6011
BstXI CCAn_nnnn'nTGG
Cuts at: 3787 4255 3787
Size: 468 7774
BstYI r'GATC_y
Cuts at: 1972 2452 2486 2593 2790 2856 3048 3672 3808
Size: 480 34 107 197 66 192 624 136
Cuts at: 3808 4457 5572 5939 6324 6341 7793 7879 7891
Size: 649 1115 367 385 17 1452 86 12
Cuts at: 7891 8237 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1115 649 624 480 385 367
346 197 192 136 107 86 66 34
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3741 4128 4752 513
Size: 552 2676 387 624 4003
Fragments arranged by size:
4003 2676 624 552 387
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4544 5506 487
Size: 683 56 3318 962 3223
Fragments arranged by size:
3318 3223 962 683 56
BtsI GCAGTG_nn'
Cuts at: 2245 2647 2887 2939 3086 4278 5448 5758 6552
Size: 402 240 52 147 1192 1170 310 794
Cuts at: 6552 6572 2245
Size: 20 3915
Fragments arranged by size:
3915 1192 1170 794 402 310 240 147
52 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8242
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8207
DraI TTT'AAA
Cuts at: 5546 5546
Size: 8242
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4839 7249 809
Size: 507 3523 2410 1802
Fragments arranged by size:
3523 2410 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2027 2076 3492
Size: 54 1425 50 275 18 197 49 1416
Cuts at: 3492 3738 3840 4275 4846 5689 6578 8207 8
Size: 246 102 435 571 843 889 1629 43
Fragments arranged by size:
1629 1425 1416 889 843 571 435 275
246 197 102 54 50 49 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3738 4846 5689 1537
Size: 275 1926 1108 843 4090
Fragments arranged by size:
4090 1926 1108 843 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3061 3500 4737 5234 5307 6172 1353
Size: 1708 439 1237 497 73 865 3423
Fragments arranged by size:
3423 1708 1237 865 497 439 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6829 7203 7349 387
Size: 1064 138 5240 374 146 1280
Fragments arranged by size:
5240 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3988 3988
Size: 8242
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2098 2403 2490 2792 2937 3046
Size: 393 401 65 305 87 302 145 109
Cuts at: 3046 3418 3431 3464 3776 3836 3944 3952 5000
Size: 372 13 33 312 60 108 8 1048
Cuts at: 5000 5052 5110 5162 5351 5458 5525 6305 6902
Size: 52 58 52 189 107 67 780 597
Cuts at: 6902 7689 7957 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1048 787 780 597 401 393 372
312 305 302 268 189 145 109 108
107 87 67 65 60 58 52 52
33 13 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2203 3707 3791 4965 2203
Size: 1504 84 1174 5480
Fragments arranged by size:
5480 1504 1174 84
EcoO109I rG'GnC_Cy
Cuts at: 2158 2947 4141 4193 4214 4477 4568 4608 4841
Size: 789 1194 52 21 263 91 40 233
Cuts at: 4841 4909 4970 5434 2158
Size: 68 61 464 4966
Fragments arranged by size:
4966 1194 789 464 263 233 91 68
61 52 40 21
EcoRI G'AATT_C
Cuts at: 3290 5551 5929 3290
Size: 2261 378 5603
Fragments arranged by size:
5603 2261 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3436 3494 4671 5120 5263 3436
Size: 58 1177 449 143 6415
Fragments arranged by size:
6415 1177 449 143 58
FspI TGC'GCA
Cuts at: 6748 6748
Size: 8242
HaeII r_GCGC'y
Cuts at: 1678 3769 3835 5258 7389 1678
Size: 2091 66 1423 2131 2531
Fragments arranged by size:
2531 2131 2091 1423 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3035 3067 4427 4459 4714
Size: 32 135 32 1463 32 1360 32 255
Cuts at: 4714 4746 5216 5248 6883 6915 6957 6989 1373
Size: 32 470 32 1635 32 42 32 2626
Fragments arranged by size:
2626 1635 1463 1360 470 255 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5586 5832 669
Size: 988 281 3648 246 3079
Fragments arranged by size:
3648 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3372 3452 4882 5422 748
Size: 2624 80 1430 540 3568
Fragments arranged by size:
3568 2624 1430 540 80
HpaI GTT'AAC
Cuts at: 5832 5832
Size: 8242
KpnI G_GTAC'C
Cuts at: 5567 5567
Size: 8242
MfeI C'AATT_G
Cuts at: 5841 5841
Size: 8242
MluI A'CGCG_T
Cuts at: 8118 8118
Size: 8242
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5287 7356 7540 1305
Size: 3982 2069 184 2007
Fragments arranged by size:
3982 2069 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2078 3494 3842 4277 10
Size: 54 1425 343 246 1416 348 435 3975
Fragments arranged by size:
3975 1425 1416 435 348 343 246 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2571 4283 6200 6559 6718 518
Size: 648 1405 1712 1917 359 159 2042
Fragments arranged by size:
2042 1917 1712 1405 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2033 2731 2916 3308 5658 6335 7483 7728
Size: 427 698 185 392 2350 677 1148 245
Cuts at: 7728 1606
Size: 2120
Fragments arranged by size:
2350 2120 1148 698 677 427 392 245
185
NcoI C'CATG_G
Cuts at: 513 1065 4128 4752 513
Size: 552 3063 624 4003
Fragments arranged by size:
4003 3063 624 552
NdeI CA'TA_TG
Cuts at: 387 3030 387
Size: 2643 5599
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8242
NotI GC'GGCC_GC
Cuts at: 5689 5689
Size: 8242
NspI r_CATG'y
Cuts at: 1132 1229 2293 4000 4761 5450 5600 7145 1132
Size: 97 1064 1707 761 689 150 1545 2229
Fragments arranged by size:
2229 1707 1545 1064 761 689 150 97
PasI CC'CwG_GG
Cuts at: 1348 2482 4264 4299 4640 4718 5004 1348
Size: 1134 1782 35 341 78 286 4586
Fragments arranged by size:
4586 1782 1134 341 286 78 35
PciI A'CATG_T
Cuts at: 7141 7141
Size: 8242
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4946 5329 1108
Size: 3838 383 4021
Fragments arranged by size:
4021 3838 383
PfoI T'CCnGG_A
Cuts at: 4982 5155 4982
Size: 173 8069
PmeI GTTT'AAAC
Cuts at: 5546 5546
Size: 8242
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6311 6343 7824 7856 6311
Size: 32 1481 32 6697
Fragments arranged by size:
6697 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2158 2947 4141 4193 4477 4568 4841 4909 4970
Size: 789 1194 52 284 91 273 68 61
Cuts at: 4970 2158
Size: 5430
Fragments arranged by size:
5430 1194 789 284 273 91 68 61
52
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8242
PsiI TTA'TAA
Cuts at: 5812 5812
Size: 8242
PspOMI G'GGCC_C
Cuts at: 4607 4607
Size: 8242
PstI C_TGCA'G
Cuts at: 830 3093 3318 3584 4140 5175 5594 830
Size: 2263 225 266 556 1035 419 3478
Fragments arranged by size:
3478 2263 1035 556 419 266 225
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8242
PvuII CAG'CTG
Cuts at: 2033 2731 2916 3308 2033
Size: 698 185 392 6967
Fragments arranged by size:
6967 698 392 185
SacI G_AGCT'C
Cuts at: 3990 3990
Size: 8242
SalI G'TCGA_C
Cuts at: 1936 5584 1936
Size: 3648 4594
SanDI GG'GwC_CC
Cuts at: 2158 2947 4568 4909 2158
Size: 789 1621 341 5491
Fragments arranged by size:
5491 1621 789 341
SapI GCTCTTCn'nnn_
Cuts at: 5234 5234
Size: 8242
SbfI CC_TGCA'GG
Cuts at: 5594 5594
Size: 8242
ScaI AGT'ACT
Cuts at: 6490 8202 6490
Size: 1712 6530
SexAI A'CCwGG_T
Cuts at: 4473 4473
Size: 8242
SfcI C'TryA_G
Cuts at: 826 1045 3089 3314 3580 3916 4136 4228 4594
Size: 219 2044 225 266 336 220 92 366
Cuts at: 4594 5171 5464 5590 6725 7406 7597 826
Size: 577 293 126 1135 681 191 1471
Fragments arranged by size:
2044 1471 1135 681 577 366 336 293
266 225 220 219 191 126 92
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8242
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8242
SmaI CCC'GGG
Cuts at: 1872 5569 1872
Size: 3697 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2021 2200 4353 4974 6346 7247
Size: 197 926 78 179 2153 621 1372 901
Cuts at: 7247 7509 820
Size: 262 1553
Fragments arranged by size:
2153 1553 1372 926 901 621 262 197
179 78
SnaBI TAC'GTA
Cuts at: 493 6033 493
Size: 5540 2702
SpeI A'CTAG_T
Cuts at: 152 2837 152
Size: 2685 5557
SphI G_CATG'C
Cuts at: 1132 2293 4761 5450 5600 1132
Size: 1161 2468 689 150 3774
Fragments arranged by size:
3774 2468 1161 689 150
SspI AAT'ATT
Cuts at: 5 52 2139 6166 5
Size: 47 2087 4027 2081
Fragments arranged by size:
4027 2087 2081 47
StuI AGG'CCT
Cuts at: 1517 2658 3323 3575 3590 4651 1517
Size: 1141 665 252 15 1061 5108
Fragments arranged by size:
5108 1141 1061 665 252 15
StyI C'CwwG_G
Cuts at: 513 1065 3324 3540 3665 4128 4493 4499 4536
Size: 552 2259 216 125 463 365 6 37
Cuts at: 4536 4752 5681 513
Size: 216 929 3074
Fragments arranged by size:
3074 2259 929 552 463 365 216 216
125 37 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2699 4980 6269 6286 6439 6624 7069
Size: 1255 1119 2281 1289 17 153 185 445
Cuts at: 7069 325
Size: 1498
Fragments arranged by size:
2281 1498 1289 1255 1119 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2994 6488 8200
Size: 274 80 33 51 1443 1017 3494 1712
Cuts at: 8200 96
Size: 138
Fragments arranged by size:
3494 1712 1443 1017 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3893 3925 8202 8234 3893
Size: 32 4277 32 3901
Fragments arranged by size:
4277 3901 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8242
XbaI T'CTAG_A
Cuts at: 5578 5578
Size: 8242
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8242
XmaI C'CCGG_G
Cuts at: 1870 5567 1870
Size: 3697 4545
XmnI GAAnn'nnTTC
Cuts at: 2396 6371 2396
Size: 3975 4267
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6050 276
Size: 53 83 186 936 4516 2468
Fragments arranged by size:
4516 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SfiI SfoI SrfI SwaI XcmI