Restriction map for
pFN21AE0054
(Circular) (Six-base) MAPSORT of: pFN21AE0054.seq Check: 7905 from: 1 to: 7744
pFN21AE0054 7744 bp
With 182 enzymes: SgfI *
September 11, 2017 14:08 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5554 278
Size: 53 83 186 936 4018 2468
Fragments arranged by size:
4018 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4701 5087 1937
Size: 2764 386 4594
Fragments arranged by size:
4594 2764 386
Acc65I G'GTAC_C
Cuts at: 5065 5065
Size: 7744
AclI AA'CG_TT
Cuts at: 1563 5871 6244 1563
Size: 4308 373 3063
Fragments arranged by size:
4308 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2166 2361 4278 4289 4609 5807 7191 7459
Size: 534 195 1917 11 320 1198 1384 268
Cuts at: 7459 1632
Size: 1917
Fragments arranged by size:
1917 1917 1384 1198 534 320 268 195
11
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7547
AflIII A'CryG_T
Cuts at: 1203 6643 7620 1203
Size: 5440 977 1327
Fragments arranged by size:
5440 1327 977
AgeI A'CCGG_T
Cuts at: 6575 6575
Size: 7744
AhdI GACnn_n'nnGTC
Cuts at: 6473 6473
Size: 7744
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7744
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3204 3420 1184
Size: 2020 216 5508
Fragments arranged by size:
5508 2020 216
AlwNI CAG_nnn'CTG
Cuts at: 3428 5487 7059 3428
Size: 2059 1572 4113
Fragments arranged by size:
4113 2059 1572
ApaI G_GGCC'C
Cuts at: 4771 4771
Size: 7744
ApaLI G'TGCA_C
Cuts at: 2777 4478 4754 5801 6957 2777
Size: 1701 276 1047 1156 3564
Fragments arranged by size:
3564 1701 1156 1047 276
ApoI r'AATT_y
Cuts at: 1493 2726 3066 3223 3236 3501 3818 5053 5279
Size: 1233 340 157 13 265 317 1235 226
Cuts at: 5279 5431 7540 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1235 1233 340 317 265 226
157 152 13
AseI AT'TA_AT
Cuts at: 160 1032 6298 160
Size: 872 5266 1606
Fragments arranged by size:
5266 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7744
AvaI C'yCGr_G
Cuts at: 1870 1943 4971 5069 1870
Size: 73 3028 98 4545
Fragments arranged by size:
4545 3028 98 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3699 3732 4128 4161 3699
Size: 33 396 33 7282
Fragments arranged by size:
7282 396 33 33
BamHI G'GATC_C
Cuts at: 5074 5441 5074
Size: 367 7377
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4529 5065 6520 618
Size: 325 217 627 2742 536 1455 1842
Fragments arranged by size:
2742 1842 1455 627 536 325 217
BanII G_rGCy'C
Cuts at: 1391 2096 2159 3404 4771 5063 1391
Size: 705 63 1245 1367 292 4072
Fragments arranged by size:
4072 1367 1245 705 292 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2808 4517 4825 928
Size: 971 909 1709 308 3847
Fragments arranged by size:
3847 1709 971 909 308
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5935 5969 1903
Size: 34 3998 34 3678
Fragments arranged by size:
3998 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5636 6846 5636
Size: 1210 6534
BclI T'GATC_A
Cuts at: 1548 4539 4951 1548
Size: 2991 412 4341
Fragments arranged by size:
4341 2991 412
BfrBI ATG'CAT
Cuts at: 2944 2944
Size: 7744
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6355 136
Size: 107 122 71 631 605 4683 1525
Fragments arranged by size:
4683 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4804 7739 4804
Size: 2935 4809
BlpI GC'TnA_GC
Cuts at: 5161 5161
Size: 7744
Bme1580I G_kGCm'C
Cuts at: 1790 2781 4482 4758 4771 5805 6961 1790
Size: 991 1701 276 13 1034 1156 2573
Fragments arranged by size:
2573 1701 1156 1034 991 276 13
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7744
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3570 4524 5475
Size: 416 53 83 399 24 2153 954 951
Cuts at: 5475 6433 442
Size: 958 1753
Fragments arranged by size:
2153 1753 958 954 951 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7744
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3161 3357 4125 4275 4516 6404 1239
Size: 1922 196 768 150 241 1888 2579
Fragments arranged by size:
2579 1922 1888 768 241 196 150
Bpu10I CC'TnA_GC
Cuts at: 4418 4418
Size: 7744
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5869 6734 7032 5869
Size: 865 298 6581
Fragments arranged by size:
6581 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2489 6407 882
Size: 617 990 3918 2219
Fragments arranged by size:
3918 2219 990 617
BsaAI yAC'GTr
Cuts at: 493 1204 5535 493
Size: 711 4331 2702
Fragments arranged by size:
4331 2702 711
BsaBI GATnn'nnATC
Cuts at: 7738 7738
Size: 7744
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5551 5933 6568 275
Size: 53 83 186 936 4018 382 635 1451
Fragments arranged by size:
4018 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4040 6177 6575 6849 6996 7407 4040
Size: 2137 398 274 147 411 4377
Fragments arranged by size:
4377 2137 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2643 2673 3778 3808 3994
Size: 30 165 30 1240 30 1105 30 186
Cuts at: 3994 4024 4273 4303 1178
Size: 30 249 30 4619
Fragments arranged by size:
4619 1240 1105 249 186 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2035 2125 3999 1186
Size: 849 90 1874 4931
Fragments arranged by size:
4931 1874 849 90
BseYI C'CCAG_C
Cuts at: 6947 7443 6947
Size: 496 7248
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4215 1163
Size: 573 2479 4692
Fragments arranged by size:
4692 2479 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5194 5955 6983 1540
Size: 275 186 3193 761 1028 2301
Fragments arranged by size:
3193 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2159 2753 2781 3134 3404 4482 4758 5805 5890
Size: 594 28 353 270 1078 276 1047 85
Cuts at: 5890 6961 2159
Size: 1071 2942
Fragments arranged by size:
2942 1078 1071 1047 594 353 276 270
85 28
BsmI GAATG_Cn'
Cuts at: 4747 5255 5348 4747
Size: 508 93 7143
Fragments arranged by size:
7143 508 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4793 4793
Size: 7744
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2159 2753 2781 3134 3404 4482
Size: 399 306 63 594 28 353 270 1078
Cuts at: 4482 4758 4771 5063 5805 5890 6961 1391
Size: 276 13 292 742 85 1071 2174
Fragments arranged by size:
2174 1078 1071 742 594 399 353 306
292 276 270 85 63 28 13
BspEI T'CCGG_A
Cuts at: 7407 7407
Size: 7744
BspHI T'CATG_A
Cuts at: 5633 7363 5633
Size: 1730 6014
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5099 844
Size: 4255 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5631 1394
Size: 429 3808 3507
Fragments arranged by size:
3808 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2873 6239 6413 65
Size: 1183 1625 3366 174 1396
Fragments arranged by size:
3366 1625 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6388 6575 1700
Size: 110 4578 187 2869
Fragments arranged by size:
4578 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4654 96
Size: 4558 3186
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7744
BssSI C'ACGA_G
Cuts at: 5804 6816 5804
Size: 1012 6732
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2546 794
Size: 1752 5992
BstBI TT'CG_AA
Cuts at: 6548 6548
Size: 7744
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7744
BstXI CCAn_nnnn'nTGG
Cuts at: 4299 4299
Size: 7744
BstYI r'GATC_y
Cuts at: 1972 2314 2974 3302 4346 4629 4804 5001 5074
Size: 342 660 328 1044 283 175 197 73
Cuts at: 5074 5441 5826 5843 7295 7381 7393 7739 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1044 660 385 367 346 342
328 283 197 175 86 73 17 12
Bsu36I CC'TnA_GG
Cuts at: 2346 2346
Size: 7744
BtgI C'CryG_G
Cuts at: 513 1065 2004 4749 513
Size: 552 939 2745 3508
Fragments arranged by size:
3508 2745 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4951 487
Size: 683 56 3725 3280
Fragments arranged by size:
3725 3280 683 56
BtsI GCAGTG_nn'
Cuts at: 5260 6054 6074 5260
Size: 794 20 6930
Fragments arranged by size:
6930 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7744
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7709
DraI TTT'AAA
Cuts at: 2731 3970 4000 5048 2731
Size: 1239 30 1048 5427
Fragments arranged by size:
5427 1239 1048 30
DraIII CAC_nnn'GTG
Cuts at: 4238 4238
Size: 7744
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6751 809
Size: 507 5435 1802
Fragments arranged by size:
5435 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 3342 4301
Size: 54 1425 50 275 18 192 1320 959
Cuts at: 4301 5191 6080 7709 8
Size: 890 889 1629 43
Fragments arranged by size:
1629 1425 1320 959 890 889 275 192
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5191 1537
Size: 275 3379 4090
Fragments arranged by size:
4090 3379 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2117 2380 2472 2910 4608 5674 1353
Size: 764 263 92 438 1698 1066 3423
Fragments arranged by size:
3423 1698 1066 764 438 263 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 6331 6705 6851 387
Size: 1064 138 435 4307 374 146 1280
Fragments arranged by size:
4307 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2157 3402 2157
Size: 1245 6499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2166 2361 3161 3357 4125 4275 4278
Size: 393 534 195 800 196 768 150 3
Cuts at: 4278 4289 4516 4609 5807 6404 7191 7459 1239
Size: 11 227 93 1198 597 787 268 1524
Fragments arranged by size:
1524 1198 800 787 768 597 534 393
268 227 196 195 150 93 11 3
EcoNI CCTnn'n_nnAGG
Cuts at: 4131 4131
Size: 7744
EcoO109I rG'GnC_Cy
Cuts at: 3904 4767 4768 3904
Size: 863 1 6880
Fragments arranged by size:
6880 863 1
EcoRI G'AATT_C
Cuts at: 3223 3236 3818 5053 5431 3223
Size: 13 582 1235 378 5536
Fragments arranged by size:
5536 1235 582 378 13
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3981 3981
Size: 7744
FspI TGC'GCA
Cuts at: 6250 6250
Size: 7744
HaeII r_GCGC'y
Cuts at: 1678 6891 1678
Size: 5213 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2162 2194 2777 2809 2959
Size: 32 135 32 590 32 583 32 150
Cuts at: 2959 2991 3878 3910 6385 6417 6459 6491 1373
Size: 32 887 32 2475 32 42 32 2626
Fragments arranged by size:
2626 2475 887 590 583 150 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4224 4813 5088 5334 669
Size: 988 281 2286 589 275 246 3079
Fragments arranged by size:
3079 2286 988 589 281 275 246
HindIII A'AGCT_T
Cuts at: 748 2825 4722 748
Size: 2077 1897 3770
Fragments arranged by size:
3770 2077 1897
HpaI GTT'AAC
Cuts at: 5334 5334
Size: 7744
KpnI G_GTAC'C
Cuts at: 5069 5069
Size: 7744
MfeI C'AATT_G
Cuts at: 4989 5343 4989
Size: 354 7390
MluI A'CGCG_T
Cuts at: 7620 7620
Size: 7744
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6858 7042 1305
Size: 5553 184 2007
Fragments arranged by size:
5553 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3344 4303 10
Size: 54 1425 343 1512 959 3451
Fragments arranged by size:
3451 1512 1425 959 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2901 3885 4032 4577 5702 6061 6220
Size: 648 1735 984 147 545 1125 359 159
Cuts at: 6220 518
Size: 2042
Fragments arranged by size:
2042 1735 1125 984 648 545 359 159
147
MspA1I CmG'CkG
Cuts at: 1606 5160 5837 6985 7230 1606
Size: 3554 677 1148 245 2120
Fragments arranged by size:
3554 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 4749 513
Size: 552 939 2745 3508
Fragments arranged by size:
3508 2745 939 552
NdeI CA'TA_TG
Cuts at: 387 4315 387
Size: 3928 3816
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7744
NotI GC'GGCC_GC
Cuts at: 5191 5191
Size: 7744
NsiI A_TGCA'T
Cuts at: 2946 2946
Size: 7744
NspI r_CATG'y
Cuts at: 1132 1229 3693 4448 5102 6647 1132
Size: 97 2464 755 654 1545 2229
Fragments arranged by size:
2464 2229 1545 755 654 97
PasI CC'CwG_GG
Cuts at: 1348 2113 1348
Size: 765 6979
PciI A'CATG_T
Cuts at: 6643 6643
Size: 7744
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7744
PmeI GTTT'AAAC
Cuts at: 5048 5048
Size: 7744
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5813 5845 7326 7358 5813
Size: 32 1481 32 6199
Fragments arranged by size:
6199 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3904 3904
Size: 7744
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7744
PsiI TTA'TAA
Cuts at: 5314 5314
Size: 7744
PspOMI G'GGCC_C
Cuts at: 4767 4767
Size: 7744
PstI C_TGCA'G
Cuts at: 830 2132 5096 830
Size: 1302 2964 3478
Fragments arranged by size:
3478 2964 1302
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7744
SacI G_AGCT'C
Cuts at: 2159 3404 2159
Size: 1245 6499
SalI G'TCGA_C
Cuts at: 1936 5086 1936
Size: 3150 4594
SapI GCTCTTCn'nnn_
Cuts at: 2117 2472 4608 2117
Size: 355 2136 5253
Fragments arranged by size:
5253 2136 355
SbfI CC_TGCA'GG
Cuts at: 5096 5096
Size: 7744
ScaI AGT'ACT
Cuts at: 2597 4266 5992 7704 2597
Size: 1669 1726 1712 2637
Fragments arranged by size:
2637 1726 1712 1669
SfcI C'TryA_G
Cuts at: 826 1045 2128 3033 4014 5092 6227 6908 7099
Size: 219 1083 905 981 1078 1135 681 191
Cuts at: 7099 826
Size: 1471
Fragments arranged by size:
1471 1135 1083 1078 981 905 681 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7744
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7744
SmaI CCC'GGG
Cuts at: 1872 5071 1872
Size: 3199 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5848 6749 7011 820
Size: 197 926 3905 901 262 1553
Fragments arranged by size:
3905 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5535 493
Size: 5042 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7744
SphI G_CATG'C
Cuts at: 1132 5102 1132
Size: 3970 3774
SspI AAT'ATT
Cuts at: 5 52 5668 5
Size: 47 5616 2081
Fragments arranged by size:
5616 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7744
StyI C'CwwG_G
Cuts at: 513 1065 2004 4132 4749 5183 513
Size: 552 939 2128 617 434 3074
Fragments arranged by size:
3074 2128 939 617 552 434
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3017 5771 5788 5941 6126 6571 325
Size: 1255 1437 2754 17 153 185 445 1498
Fragments arranged by size:
2754 1498 1437 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2595 4264 4654
Size: 274 80 33 51 1443 618 1669 390
Cuts at: 4654 4935 5990 7702 96
Size: 281 1055 1712 138
Fragments arranged by size:
1712 1669 1443 1055 618 390 281 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4273 4305 7704 7736 4273
Size: 32 3399 32 4281
Fragments arranged by size:
4281 3399 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4984 1316
Size: 3668 4076
XbaI T'CTAG_A
Cuts at: 2320 5080 2320
Size: 2760 4984
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3352 3352
Size: 7744
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7744
XmaI C'CCGG_G
Cuts at: 1870 5069 1870
Size: 3199 4545
XmnI GAAnn'nnTTC
Cuts at: 5873 5873
Size: 7744
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5552 276
Size: 53 83 186 936 4018 2468
Fragments arranged by size:
4018 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BplI
BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI