Restriction map for
pFN21AE0053
(Circular) (Six-base) MAPSORT of: pFN21AE0053.seq Check: 2912 from: 1 to: 9409
pFN21AE0053 9409 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2919 6219 7219 278
Size: 53 83 186 936 1383 3300 1000 2468
Fragments arranged by size:
3300 2468 1383 1000 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5304 6071 6752 1937
Size: 3367 767 681 4594
Fragments arranged by size:
4594 3367 767 681
Acc65I G'GTAC_C
Cuts at: 2529 6730 2529
Size: 4201 5208
AclI AA'CG_TT
Cuts at: 1563 7536 7909 1563
Size: 5973 373 3063
Fragments arranged by size:
5973 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2302 2980 5139 5741 5919 6293 7472 8856
Size: 670 678 2159 602 178 374 1179 1384
Cuts at: 8856 9124 1632
Size: 268 1917
Fragments arranged by size:
2159 1917 1384 1179 678 670 602 374
268 178
AflII C'TTAA_G
Cuts at: 820 1017 3203 5728 820
Size: 197 2186 2525 4501
Fragments arranged by size:
4501 2525 2186 197
AflIII A'CryG_T
Cuts at: 1203 8308 9285 1203
Size: 7105 977 1327
Fragments arranged by size:
7105 1327 977
AgeI A'CCGG_T
Cuts at: 8240 8240
Size: 9409
AhdI GACnn_n'nnGTC
Cuts at: 8138 8138
Size: 9409
AleI CACnn'nnGTG
Cuts at: 1166 2459 2552 2819 1166
Size: 1293 93 267 7756
Fragments arranged by size:
7756 1293 267 93
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2345 2459 2837 3535 1184
Size: 1161 114 378 698 7058
Fragments arranged by size:
7058 1161 698 378 114
AlwNI CAG_nnn'CTG
Cuts at: 2662 3914 4110 6212 7152 8724 2662
Size: 1252 196 2102 940 1572 3347
Fragments arranged by size:
3347 2102 1572 1252 940 196
ApaLI G'TGCA_C
Cuts at: 3445 7466 8622 3445
Size: 4021 1156 4232
Fragments arranged by size:
4232 4021 1156
ApoI r'AATT_y
Cuts at: 1493 3223 3351 3528 3748 4163 4336 4590 5588
Size: 1730 128 177 220 415 173 254 998
Cuts at: 5588 5926 6509 6545 6573 6718 6944 7096 9205
Size: 338 583 36 28 145 226 152 2109
Cuts at: 9205 1493
Size: 1697
Fragments arranged by size:
2109 1730 1697 998 583 415 338 254
226 220 177 173 152 145 128 36
28
AseI AT'TA_AT
Cuts at: 160 1032 7963 160
Size: 872 6931 1606
Fragments arranged by size:
6931 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9409
AvaI C'yCGr_G
Cuts at: 1870 1943 2306 2360 4068 6734 1870
Size: 73 363 54 1708 2666 4545
Fragments arranged by size:
4545 2666 1708 363 73 54
BamHI G'GATC_C
Cuts at: 4241 5894 6739 7106 4241
Size: 1653 845 367 6544
Fragments arranged by size:
6544 1653 845 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2026 2284 2399 2453 2529
Size: 325 217 627 239 258 115 54 76
Cuts at: 2529 2667 3146 3426 6730 8185 618
Size: 138 479 280 3304 1455 1842
Fragments arranged by size:
3304 1842 1455 627 479 325 280 258
239 217 138 115 76 54
BanII G_rGCy'C
Cuts at: 1391 2118 2486 3639 4806 4968 5472 6145 6728
Size: 727 368 1153 1167 162 504 673 583
Cuts at: 6728 1391
Size: 4072
Fragments arranged by size:
4072 1167 1153 727 673 583 504 368
162
BbeI G_GCGC'C
Cuts at: 2030 2288 2403 2671 2030
Size: 258 115 268 8768
Fragments arranged by size:
8768 268 258 115
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3929 3946 3979 5568 6311 928
Size: 971 2030 17 33 1589 743 4026
Fragments arranged by size:
4026 2030 1589 971 743 33 17
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5359 5393 7600 7634 1903
Size: 34 3422 34 2207 34 3678
Fragments arranged by size:
3678 3422 2207 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3860 4217 7301 8511 3860
Size: 357 3084 1210 4758
Fragments arranged by size:
4758 3084 1210 357
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9409
BfrBI ATG'CAT
Cuts at: 5128 6359 5128
Size: 1231 8178
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2122 2153 8020
Size: 107 122 71 631 605 450 31 5867
Cuts at: 8020 136
Size: 1525
Fragments arranged by size:
5867 1525 631 605 450 122 107 71
31
BglII A'GATC_T
Cuts at: 4705 9404 4705
Size: 4699 4710
BlpI GC'TnA_GC
Cuts at: 4995 6826 4995
Size: 1831 7578
Bme1580I G_kGCm'C
Cuts at: 1790 3449 3524 5795 7470 8626 1790
Size: 1659 75 2271 1675 1156 2573
Fragments arranged by size:
2573 2271 1675 1659 1156 75
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9409
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4431 7140 8098
Size: 416 53 83 399 24 3014 2709 958
Cuts at: 8098 442
Size: 1753
Fragments arranged by size:
3014 2709 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9409
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2293 2983 2293
Size: 690 8719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2240 2878 2967 3207 3528 4820 8069 1239
Size: 1001 638 89 240 321 1292 3249 2579
Fragments arranged by size:
3249 2579 1292 1001 638 321 240 89
Bpu10I CC'TnA_GC
Cuts at: 2429 2562 2429
Size: 133 9276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2609 2657 5798 7534 8399 8697 2609
Size: 48 3141 1736 865 298 3321
Fragments arranged by size:
3321 3141 1736 865 298 48
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2017 2281 8072 882
Size: 617 518 264 5791 2219
Fragments arranged by size:
5791 2219 617 518 264
BsaAI yAC'GTr
Cuts at: 493 1204 2554 4982 7200 493
Size: 711 1350 2428 2218 2702
Fragments arranged by size:
2702 2428 2218 1350 711
BsaBI GATnn'nnATC
Cuts at: 4246 9403 4246
Size: 5157 4252
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2027 2285 2400 2668
Size: 53 83 186 936 494 258 115 268
Cuts at: 2668 2712 2916 5139 6180 6216 7216 7598 8233
Size: 44 204 2223 1041 36 1000 382 635
Cuts at: 8233 275
Size: 1451
Fragments arranged by size:
2223 1451 1041 1000 936 635 494 382
268 258 204 186 115 83 53 44
36
BsaWI w'CCGG_w
Cuts at: 3663 4968 5584 7842 8240 8514 8661 9072 3663
Size: 1305 616 2258 398 274 147 411 4000
Fragments arranged by size:
4000 2258 1305 616 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3340 3370 3450 3480 5813
Size: 30 165 30 1937 30 80 30 2333
Cuts at: 5813 5843 1178
Size: 30 4744
Fragments arranged by size:
4744 2333 1937 165 80 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2876 2881 3361 3948 5402 1186
Size: 1690 5 480 587 1454 5193
Fragments arranged by size:
5193 1690 1454 587 480 5
BseYI C'CCAG_C
Cuts at: 2662 2789 3036 3332 3799 4789 8612 9108 2662
Size: 127 247 296 467 990 3823 496 2963
Fragments arranged by size:
3823 2963 990 496 467 296 247 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2248 2684 3655 5664 1163
Size: 573 512 436 971 2009 4908
Fragments arranged by size:
4908 2009 971 573 512 436
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2101 2129 2355 2395 2493 2875
Size: 275 186 100 28 226 40 98 382
Cuts at: 2875 2943 3054 4890 6859 7620 8648 1540
Size: 68 111 1836 1969 761 1028 2301
Fragments arranged by size:
2301 1969 1836 1028 761 382 275 226
186 111 100 98 68 40 28
BsiHKAI G_wGCw'C
Cuts at: 2226 2816 3449 3639 4806 4857 4968 6145 7470
Size: 590 633 190 1167 51 111 1177 1325
Cuts at: 7470 7555 8626 2226
Size: 85 1071 3009
Fragments arranged by size:
3009 1325 1177 1167 1071 633 590 190
111 85 51
BsmI GAATG_Cn'
Cuts at: 3800 5723 5990 6347 6920 7013 3800
Size: 1923 267 357 573 93 6196
Fragments arranged by size:
6196 1923 573 357 267 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2564 5130 5377 2564
Size: 2566 247 6596
Fragments arranged by size:
6596 2566 247
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2226 2486 2816 3449 3524 3639
Size: 399 328 108 260 330 633 75 115
Cuts at: 3639 4806 4857 4968 5472 5795 6145 6728 7470
Size: 1167 51 111 504 323 350 583 742
Cuts at: 7470 7555 8626 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1167 1071 742 633 583 504 399
350 330 328 323 260 115 111 108
85 75 51
BspEI T'CCGG_A
Cuts at: 4968 5584 9072 4968
Size: 616 3488 5305
Fragments arranged by size:
5305 3488 616
BspHI T'CATG_A
Cuts at: 7298 9028 7298
Size: 1730 7679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3677 5569 6764 844
Size: 2833 1892 1195 3489
Fragments arranged by size:
3489 2833 1892 1195
BsrBI CCG'CTC
Cuts at: 1394 1823 2187 2769 2962 7296 1394
Size: 429 364 582 193 4334 3507
Fragments arranged by size:
4334 3507 582 429 364 193
BsrDI GCAATG_nn'
Cuts at: 65 1248 4254 4755 5280 7904 8078 65
Size: 1183 3006 501 525 2624 174 1396
Fragments arranged by size:
3006 2624 1396 1183 525 501 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2272 2354 2378 2492 2780 8053 8240
Size: 110 462 82 24 114 288 5273 187
Cuts at: 8240 1700
Size: 2869
Fragments arranged by size:
5273 2869 462 288 187 114 110 82
24
BsrGI T'GTAC_A
Cuts at: 96 3466 5547 96
Size: 3370 2081 3958
Fragments arranged by size:
3958 3370 2081
BssHII G'CGCG_C
Cuts at: 1925 2798 3017 3019 1925
Size: 873 219 2 8315
Fragments arranged by size:
8315 873 219 2
BssSI C'ACGA_G
Cuts at: 7469 8481 7469
Size: 1012 8397
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9409
BstBI TT'CG_AA
Cuts at: 3541 8213 3541
Size: 4672 4737
BstEII G'GTnAC_C
Cuts at: 1184 4897 1184
Size: 3713 5696
BstXI CCAn_nnnn'nTGG
Cuts at: 2902 4217 2902
Size: 1315 8094
BstYI r'GATC_y
Cuts at: 1972 3009 4241 4705 5894 6208 6739 7106 7491
Size: 1037 1232 464 1189 314 531 367 385
Cuts at: 7491 7508 8960 9046 9058 9404 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1232 1189 1037 531 464 385
367 346 314 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2592 2857 2979 3150 4651 4890
Size: 552 939 588 265 122 171 1501 239
Cuts at: 4890 5520 513
Size: 630 4402
Fragments arranged by size:
4402 1501 939 630 588 552 265 239
171 122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2134 2602 2788 487
Size: 683 56 908 468 186 7108
Fragments arranged by size:
7108 908 683 468 186 56
BtsI GCAGTG_nn'
Cuts at: 2057 3061 3415 5285 5959 6925 7719 7739 2057
Size: 1004 354 1870 674 966 794 20 3727
Fragments arranged by size:
3727 1870 1004 966 794 674 354 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9409
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9374
DraI TTT'AAA
Cuts at: 4443 6713 4443
Size: 2270 7139
DraIII CAC_nnn'GTG
Cuts at: 2707 2707
Size: 9409
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8416 809
Size: 507 7100 1802
Fragments arranged by size:
7100 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 2352 2490
Size: 54 1425 50 275 18 268 254 138
Cuts at: 2490 2541 2613 2649 2854 2860 2872 2940 3051
Size: 51 72 36 205 6 12 68 111
Cuts at: 3051 3193 4887 5695 5872 6856 7745 9374 8
Size: 142 1694 808 177 984 889 1629 43
Fragments arranged by size:
1694 1629 1425 984 889 808 275 268
254 205 177 142 138 111 72 68
54 51 50 43 36 18 12 6
EagI C'GGCC_G
Cuts at: 1537 1812 2098 2352 2490 2872 2940 3051 4887
Size: 275 286 254 138 382 68 111 1836
Cuts at: 4887 6856 1537
Size: 1969 4090
Fragments arranged by size:
4090 1969 1836 382 286 275 254 138
111 68
EarI CTCTTCn'nnn_
Cuts at: 1353 2999 3261 4176 4237 4652 4771 6255 7339
Size: 1646 262 915 61 415 119 1484 1084
Cuts at: 7339 1353
Size: 3423
Fragments arranged by size:
3423 1646 1484 1084 915 415 262 119
61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7996 8370 8516 387
Size: 1064 138 6407 374 146 1280
Fragments arranged by size:
6407 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3637 4804 4966 6143 3637
Size: 1167 162 1177 6903
Fragments arranged by size:
6903 1177 1167 162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 2302 2878 2967 2980 3207 3528
Size: 393 608 62 576 89 13 227 321
Cuts at: 3528 4820 5139 5741 5919 6293 7472 8069 8856
Size: 1292 319 602 178 374 1179 597 787
Cuts at: 8856 9124 1239
Size: 268 1524
Fragments arranged by size:
1524 1292 1179 787 608 602 597 576
393 374 321 319 268 227 178 89
62 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2903 5091 2903
Size: 2188 7221
EcoO109I rG'GnC_Cy
Cuts at: 2908 4531 5516 2908
Size: 1623 985 6801
Fragments arranged by size:
6801 1623 985
EcoRI G'AATT_C
Cuts at: 3351 5588 6718 7096 3351
Size: 2237 1130 378 5664
Fragments arranged by size:
5664 2237 1130 378
EcoRV GAT'ATC
Cuts at: 5886 6427 5886
Size: 541 8868
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3465 3465
Size: 9409
FseI GG_CCGG'CC
Cuts at: 2358 2358
Size: 9409
FspI TGC'GCA
Cuts at: 2447 4460 7915 2447
Size: 2013 3455 3941
Fragments arranged by size:
3941 3455 2013
FspAI rTGC'GCAy
Cuts at: 4460 4460
Size: 9409
HaeII r_GCGC'y
Cuts at: 1678 2030 2057 2288 2403 2671 2775 2968 2987
Size: 352 27 231 115 268 104 193 19
Cuts at: 2987 8556 1678
Size: 5569 2531
Fragments arranged by size:
5569 2531 352 268 231 193 115 104
27 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2450 2482 3119 3151 3488
Size: 32 135 32 878 32 637 32 337
Cuts at: 3488 3520 3911 3943 4805 4837 5813 5845 8050
Size: 32 391 32 862 32 976 32 2205
Cuts at: 8050 8082 8124 8156 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2205 976 878 862 637 391 337
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3721 4940 6072 6753 6999 669
Size: 988 281 1783 1219 1132 681 246 3079
Fragments arranged by size:
3079 1783 1219 1132 988 681 281 246
HindIII A'AGCT_T
Cuts at: 748 3580 5194 6407 748
Size: 2832 1614 1213 3750
Fragments arranged by size:
3750 2832 1614 1213
HpaI GTT'AAC
Cuts at: 6999 6999
Size: 9409
KasI G'GCGC_C
Cuts at: 2026 2284 2399 2667 2026
Size: 258 115 268 8768
Fragments arranged by size:
8768 268 258 115
KpnI G_GTAC'C
Cuts at: 2533 6734 2533
Size: 4201 5208
MfeI C'AATT_G
Cuts at: 7008 7008
Size: 9409
MluI A'CGCG_T
Cuts at: 9285 9285
Size: 9409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2109 2625 3118 3150 5443 5636 8523 8707
Size: 804 516 493 32 2293 193 2887 184
Cuts at: 8707 1305
Size: 2007
Fragments arranged by size:
2887 2293 2007 804 516 493 193 184
32
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2651 3195 5697 5874 10
Size: 54 1425 343 819 544 2502 177 3545
Fragments arranged by size:
3545 2502 1425 819 544 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2459 2552 2819 4085 4157 6540 7367
Size: 648 1293 93 267 1266 72 2383 827
Cuts at: 7367 7726 7885 518
Size: 359 159 2042
Fragments arranged by size:
2383 2042 1293 1266 827 648 359 267
159 93 72
MspA1I CmG'CkG
Cuts at: 1606 2193 2206 2526 2583 2594 2945 3036 3332
Size: 587 13 320 57 11 351 91 296
Cuts at: 3332 6825 7502 8650 8895 1606
Size: 3493 677 1148 245 2120
Fragments arranged by size:
3493 2120 1148 677 587 351 320 296
245 91 57 13 11
NaeI GCC'GGC
Cuts at: 2356 2494 2356
Size: 138 9271
NarI GG'CG_CC
Cuts at: 2027 2285 2400 2668 2027
Size: 258 115 268 8768
Fragments arranged by size:
8768 268 258 115
NcoI C'CATG_G
Cuts at: 513 1065 2004 4651 513
Size: 552 939 2647 5271
Fragments arranged by size:
5271 2647 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9409
NgoMIV G'CCGG_C
Cuts at: 2354 2492 2354
Size: 138 9271
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9409
NotI GC'GGCC_GC
Cuts at: 2940 6856 2940
Size: 3916 5493
NsiI A_TGCA'T
Cuts at: 5130 6361 5130
Size: 1231 8178
NspI r_CATG'y
Cuts at: 1132 1229 3807 5365 6668 6767 8312 1132
Size: 97 2578 1558 1303 99 1545 2229
Fragments arranged by size:
2578 2229 1558 1545 1303 99 97
PasI CC'CwG_GG
Cuts at: 1348 2419 1348
Size: 1071 8338
PciI A'CATG_T
Cuts at: 8308 8308
Size: 9409
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2784 1108
Size: 1676 7733
PfoI T'CCnGG_A
Cuts at: 2015 5451 5628 2015
Size: 3436 177 5796
Fragments arranged by size:
5796 3436 177
PmeI GTTT'AAAC
Cuts at: 6713 6713
Size: 9409
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2810 2842 7478 7510 8991 9023 2810
Size: 32 4636 32 1481 32 3196
Fragments arranged by size:
4636 3196 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4531 5516 4531
Size: 985 8424
PshAI GACnn'nnGTC
Cuts at: 1613 5256 1613
Size: 3643 5766
PsiI TTA'TAA
Cuts at: 6979 6979
Size: 9409
PstI C_TGCA'G
Cuts at: 830 4521 5004 6761 830
Size: 3691 483 1757 3478
Fragments arranged by size:
3691 3478 1757 483
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9409
PvuII CAG'CTG
Cuts at: 2193 2206 3036 3332 2193
Size: 13 830 296 8270
Fragments arranged by size:
8270 830 296 13
SacI G_AGCT'C
Cuts at: 3639 4806 4968 6145 3639
Size: 1167 162 1177 6903
Fragments arranged by size:
6903 1177 1167 162
SacII CC_GC'GG
Cuts at: 2595 2595
Size: 9409
SalI G'TCGA_C
Cuts at: 1936 6070 6751 1936
Size: 4134 681 4594
Fragments arranged by size:
4594 4134 681
SanDI GG'GwC_CC
Cuts at: 5516 5516
Size: 9409
SapI GCTCTTCn'nnn_
Cuts at: 4237 4237
Size: 9409
SbfI CC_TGCA'GG
Cuts at: 6761 6761
Size: 9409
ScaI AGT'ACT
Cuts at: 5596 5948 7657 9369 5596
Size: 352 1709 1712 5636
Fragments arranged by size:
5636 1712 1709 352
SfcI C'TryA_G
Cuts at: 826 1045 2212 3561 3676 4517 5000 6757 7892
Size: 219 1167 1349 115 841 483 1757 1135
Cuts at: 7892 8573 8764 826
Size: 681 191 1471
Fragments arranged by size:
1757 1471 1349 1167 1135 841 681 483
219 191 115
SfoI GGC'GCC
Cuts at: 2028 2286 2401 2669 2028
Size: 258 115 268 8768
Fragments arranged by size:
8768 268 258 115
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9409
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9409
SmaI CCC'GGG
Cuts at: 1872 2308 4070 6736 1872
Size: 436 1762 2666 4545
Fragments arranged by size:
4545 2666 1762 436
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2624 2672 3203 5728 5777 7513
Size: 197 926 681 48 531 2525 49 1736
Cuts at: 7513 8414 8676 820
Size: 901 262 1553
Fragments arranged by size:
2525 1736 1553 926 901 681 531 262
197 49 48
SnaBI TAC'GTA
Cuts at: 493 7200 493
Size: 6707 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9409
SphI G_CATG'C
Cuts at: 1132 3807 6767 1132
Size: 2675 2960 3774
Fragments arranged by size:
3774 2960 2675
SspI AAT'ATT
Cuts at: 5 52 4403 4618 4690 5662 6596 7333 5
Size: 47 4351 215 72 972 934 737 2081
Fragments arranged by size:
4351 2081 972 934 737 215 72 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9409
StyI C'CwwG_G
Cuts at: 513 1065 2004 3945 4651 6075 6420 6848 513
Size: 552 939 1941 706 1424 345 428 3074
Fragments arranged by size:
3074 1941 1424 939 706 552 428 345
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4169 5120 7436 7453 7606 7791 8236
Size: 1255 2589 951 2316 17 153 185 445
Cuts at: 8236 325
Size: 1498
Fragments arranged by size:
2589 2316 1498 1255 951 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3466 4486 5547
Size: 274 80 33 51 1443 1489 1020 1061
Cuts at: 5547 5594 5946 7655 9367 96
Size: 47 352 1709 1712 138
Fragments arranged by size:
1712 1709 1489 1443 1061 1020 352 274
138 80 51 47 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3340 3372 4291 4323 9369 9401 3340
Size: 32 919 32 5046 32 3348
Fragments arranged by size:
5046 3348 919 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9409
XbaI T'CTAG_A
Cuts at: 6745 6745
Size: 9409
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2659 4217 4658 5341 2659
Size: 1558 441 683 6727
Fragments arranged by size:
6727 1558 683 441
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9409
XmaI C'CCGG_G
Cuts at: 1870 2306 4068 6734 1870
Size: 436 1762 2666 4545
Fragments arranged by size:
4545 2666 1762 436
XmnI GAAnn'nnTTC
Cuts at: 7538 7538
Size: 9409
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2917 6217 7217 276
Size: 53 83 186 936 1383 3300 1000 2468
Fragments arranged by size:
3300 2468 1383 1000 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BsiWI BstZ17I Bsu36I NruI PacI PmlI PspOMI PspXI
PsrI RsrII SexAI SfiI SrfI SwaI