Restriction map for
pFN21AE0049
(Circular) (Six-base) MAPSORT of: pFN21AE0049.seq Check: 6830 from: 1 to: 6886
pFN21AE0049 6886 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4696 278
Size: 53 83 186 936 3160 2468
Fragments arranged by size:
3160 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4229 1937
Size: 2292 4594
Acc65I G'GTAC_C
Cuts at: 4207 4207
Size: 6886
AclI AA'CG_TT
Cuts at: 1563 5013 5386 1563
Size: 3450 373 3063
Fragments arranged by size:
3450 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2042 4949 6333 6601 1632
Size: 410 2907 1384 268 1917
Fragments arranged by size:
2907 1917 1384 410 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6689
AflIII A'CryG_T
Cuts at: 1203 4000 5785 6762 1203
Size: 2797 1785 977 1327
Fragments arranged by size:
2797 1785 1327 977
AgeI A'CCGG_T
Cuts at: 5717 5717
Size: 6886
AhdI GACnn_n'nnGTC
Cuts at: 5615 5615
Size: 6886
AleI CACnn'nnGTG
Cuts at: 1166 2655 3631 3633 1166
Size: 1489 976 2 4419
Fragments arranged by size:
4419 1489 976 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2673 1184
Size: 1489 5397
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2940 2972 2940
Size: 32 6854
AlwNI CAG_nnn'CTG
Cuts at: 2969 4629 6201 2969
Size: 1660 1572 3654
Fragments arranged by size:
3654 1660 1572
ApaLI G'TGCA_C
Cuts at: 4943 6099 4943
Size: 1156 5730
ApoI r'AATT_y
Cuts at: 1493 2600 3294 3977 4109 4152 4195 4421 4573
Size: 1107 694 683 132 43 43 226 152
Cuts at: 4573 6682 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1107 694 683 226 152 132
43 43
AseI AT'TA_AT
Cuts at: 160 1032 5440 160
Size: 872 4408 1606
Fragments arranged by size:
4408 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
AvaI C'yCGr_G
Cuts at: 1870 1943 4211 1870
Size: 73 2268 4545
Fragments arranged by size:
4545 2268 73
BamHI G'GATC_C
Cuts at: 4174 4216 4583 4174
Size: 42 367 6477
Fragments arranged by size:
6477 367 42
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2657 4207 5662 618
Size: 325 217 627 870 1550 1455 1842
Fragments arranged by size:
1842 1550 1455 870 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4019 4205 1391
Size: 2628 186 4072
Fragments arranged by size:
4072 2628 186
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4104 928
Size: 971 2205 3710
Fragments arranged by size:
3710 2205 971
BbvCI CC'TCA_GC
Cuts at: 2883 2883
Size: 6886
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5077 5111 1903
Size: 34 3140 34 3678
Fragments arranged by size:
3678 3140 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4778 5988 4778
Size: 1210 5676
BclI T'GATC_A
Cuts at: 1548 2572 1548
Size: 1024 5862
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5497 136
Size: 107 122 71 631 605 3825 1525
Fragments arranged by size:
3825 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6881 6881
Size: 6886
BlpI GC'TnA_GC
Cuts at: 4303 4303
Size: 6886
Bme1580I G_kGCm'C
Cuts at: 1790 3325 4947 6103 1790
Size: 1535 1622 1156 2573
Fragments arranged by size:
2573 1622 1535 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6886
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2190 2540 3863
Size: 416 53 83 399 24 773 350 1323
Cuts at: 3863 4617 5575 442
Size: 754 958 1753
Fragments arranged by size:
1753 1323 958 773 754 416 399 350
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6886
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2655 2694 3286 3361 5546 1239
Size: 1416 39 592 75 2185 2579
Fragments arranged by size:
2579 2185 1416 592 75 39
Bpu10I CC'TnA_GC
Cuts at: 2751 2883 2751
Size: 132 6754
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2598 5011 5876 6174 2598
Size: 2413 865 298 3310
Fragments arranged by size:
3310 2413 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3264 4088 5549 882
Size: 617 1765 824 1461 2219
Fragments arranged by size:
2219 1765 1461 824 617
BsaAI yAC'GTr
Cuts at: 493 1204 3326 4677 493
Size: 711 2122 1351 2702
Fragments arranged by size:
2702 2122 1351 711
BsaBI GATnn'nnATC
Cuts at: 6880 6880
Size: 6886
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4693 5075 5710 275
Size: 53 83 186 936 3160 382 635 1451
Fragments arranged by size:
3160 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5319 5717 5991 6138 6549 5319
Size: 398 274 147 411 5656
Fragments arranged by size:
5656 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2940 2970 1178
Size: 30 165 30 1537 30 5094
Fragments arranged by size:
5094 1537 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6886
BseYI C'CCAG_C
Cuts at: 3239 6089 6585 3239
Size: 2850 496 3540
Fragments arranged by size:
3540 2850 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2051 1163
Size: 573 315 5998
Fragments arranged by size:
5998 573 315
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4336 5097 6125 1540
Size: 275 186 2335 761 1028 2301
Fragments arranged by size:
2335 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2178 2752 3867 4947 5032 6103 2178
Size: 574 1115 1080 85 1071 2961
Fragments arranged by size:
2961 1115 1080 1071 574 85
BsmI GAATG_Cn'
Cuts at: 3739 4397 4490 3739
Size: 658 93 6135
Fragments arranged by size:
6135 658 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2178 2752 3325 3867 4019 4205 4947
Size: 399 388 574 573 542 152 186 742
Cuts at: 4947 5032 6103 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 574 573 542 399 388
186 152 85
BspEI T'CCGG_A
Cuts at: 6549 6549
Size: 6886
BspHI T'CATG_A
Cuts at: 4775 6505 4775
Size: 1730 5156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4241 844
Size: 3397 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4773 1394
Size: 429 2950 3507
Fragments arranged by size:
3507 2950 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2325 2739 3748 5381 5555 65
Size: 1183 1077 414 1009 1633 174 1396
Fragments arranged by size:
1633 1396 1183 1077 1009 414 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5530 5717 1700
Size: 110 3720 187 2869
Fragments arranged by size:
3720 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6886
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6886
BssSI C'ACGA_G
Cuts at: 3629 4946 5958 3629
Size: 1317 1012 4557
Fragments arranged by size:
4557 1317 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3020 794
Size: 2226 4660
BstBI TT'CG_AA
Cuts at: 2605 5690 2605
Size: 3085 3801
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6886
BstXI CCAn_nnnn'nTGG
Cuts at: 2320 2320
Size: 6886
BstYI r'GATC_y
Cuts at: 1972 2518 3052 3379 4174 4216 4583 4968 4985
Size: 546 534 327 795 42 367 385 17
Cuts at: 4985 6437 6523 6535 6881 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 795 546 534 385 367 346
327 86 42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2234 513
Size: 552 1169 5165
Fragments arranged by size:
5165 1169 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6147
Fragments arranged by size:
6147 683 56
BtsI GCAGTG_nn'
Cuts at: 2631 2970 4402 5196 5216 2631
Size: 339 1432 794 20 4301
Fragments arranged by size:
4301 1432 794 339 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6886
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6851
DraI TTT'AAA
Cuts at: 3982 4190 3982
Size: 208 6678
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5893 809
Size: 507 4577 1802
Fragments arranged by size:
4577 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2162 2310 3448
Size: 54 1425 50 275 18 332 148 1138
Cuts at: 3448 4333 5222 6851 8
Size: 885 889 1629 43
Fragments arranged by size:
1629 1425 1138 889 885 332 275 148
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4333 1537
Size: 275 2521 4090
Fragments arranged by size:
4090 2521 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2080 3100 3461 3855 4816 1353
Size: 727 1020 361 394 961 3423
Fragments arranged by size:
3423 1020 961 727 394 361
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5473 5847 5993 387
Size: 1064 138 3884 374 146 1280
Fragments arranged by size:
3884 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2655 2694 3286 3361 4949 5546
Size: 393 410 613 39 592 75 1588 597
Cuts at: 5546 6333 6601 1239
Size: 787 268 1524
Fragments arranged by size:
1588 1524 787 613 597 592 410 393
268 75 39
EcoNI CCTnn'n_nnAGG
Cuts at: 3282 3282
Size: 6886
EcoO109I rG'GnC_Cy
Cuts at: 2508 2541 2508
Size: 33 6853
EcoRI G'AATT_C
Cuts at: 4195 4573 4195
Size: 378 6508
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3159 3314 3159
Size: 155 6731
FspI TGC'GCA
Cuts at: 5392 5392
Size: 6886
HaeII r_GCGC'y
Cuts at: 1678 6033 1678
Size: 4355 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5527 5559 5601 5633 1373
Size: 32 135 32 3955 32 42 32 2626
Fragments arranged by size:
3955 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3337 3688 4230 4476 669
Size: 988 281 1399 351 542 246 3079
Fragments arranged by size:
3079 1399 988 542 351 281 246
HindIII A'AGCT_T
Cuts at: 748 2349 748
Size: 1601 5285
HpaI GTT'AAC
Cuts at: 3688 4476 3688
Size: 788 6098
KpnI G_GTAC'C
Cuts at: 4211 4211
Size: 6886
MfeI C'AATT_G
Cuts at: 3222 4056 4485 3222
Size: 834 429 5623
Fragments arranged by size:
5623 834 429
MluI A'CGCG_T
Cuts at: 6762 6762
Size: 6886
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2007 3775 6000 6184 1305
Size: 702 1768 2225 184 2007
Fragments arranged by size:
2225 2007 1768 702 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2164 2312 3450 10
Size: 54 1425 343 332 148 1138 3446
Fragments arranged by size:
3446 1425 1138 343 332 148 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2403 2655 3248 3631 3633 4044 4844
Size: 648 1237 252 593 383 2 411 800
Cuts at: 4844 5203 5362 518
Size: 359 159 2042
Fragments arranged by size:
2042 1237 800 648 593 411 383 359
252 159 2
MspA1I CmG'CkG
Cuts at: 1606 3761 4302 4979 6127 6372 1606
Size: 2155 541 677 1148 245 2120
Fragments arranged by size:
2155 2120 1148 677 541 245
NcoI C'CATG_G
Cuts at: 513 1065 2234 513
Size: 552 1169 5165
Fragments arranged by size:
5165 1169 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6886
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6886
NotI GC'GGCC_GC
Cuts at: 4333 4333
Size: 6886
NspI r_CATG'y
Cuts at: 1132 1229 2720 2831 3247 4004 4244 5789 1132
Size: 97 1491 111 416 757 240 1545 2229
Fragments arranged by size:
2229 1545 1491 757 416 240 111 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6886
PciI A'CATG_T
Cuts at: 4000 5785 4000
Size: 1785 5101
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6886
PfoI T'CCnGG_A
Cuts at: 2710 4177 2710
Size: 1467 5419
PmeI GTTT'AAAC
Cuts at: 4190 4190
Size: 6886
PmlI CAC'GTG
Cuts at: 3326 3326
Size: 6886
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2940 2972 4955 4987 6468 6500 2940
Size: 32 1983 32 1481 32 3326
Fragments arranged by size:
3326 1983 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2541 2541
Size: 6886
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6886
PsiI TTA'TAA
Cuts at: 4456 4456
Size: 6886
PstI C_TGCA'G
Cuts at: 830 2788 3286 3944 4238 830
Size: 1958 498 658 294 3478
Fragments arranged by size:
3478 1958 658 498 294
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6886
PvuII CAG'CTG
Cuts at: 3761 3761
Size: 6886
SalI G'TCGA_C
Cuts at: 1936 4228 1936
Size: 2292 4594
SapI GCTCTTCn'nnn_
Cuts at: 2080 3855 2080
Size: 1775 5111
SbfI CC_TGCA'GG
Cuts at: 4238 4238
Size: 6886
ScaI AGT'ACT
Cuts at: 5134 6846 5134
Size: 1712 5174
SfcI C'TryA_G
Cuts at: 826 1045 2784 2811 3120 3282 3940 4234 5369
Size: 219 1739 27 309 162 658 294 1135
Cuts at: 5369 6050 6241 826
Size: 681 191 1471
Fragments arranged by size:
1739 1471 1135 681 658 309 294 219
191 162 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6886
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6886
SmaI CCC'GGG
Cuts at: 1872 4213 1872
Size: 2341 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2613 4990 5891 6153 820
Size: 197 926 670 2377 901 262 1553
Fragments arranged by size:
2377 1553 926 901 670 262 197
SnaBI TAC'GTA
Cuts at: 493 4677 493
Size: 4184 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6886
SphI G_CATG'C
Cuts at: 1132 2831 3247 4244 1132
Size: 1699 416 997 3774
Fragments arranged by size:
3774 1699 997 416
SspI AAT'ATT
Cuts at: 5 52 4810 5
Size: 47 4758 2081
Fragments arranged by size:
4758 2081 47
StuI AGG'CCT
Cuts at: 1517 3233 1517
Size: 1716 5170
StyI C'CwwG_G
Cuts at: 513 1065 2234 2537 3317 4325 513
Size: 552 1169 303 780 1008 3074
Fragments arranged by size:
3074 1169 1008 780 552 303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3653 3882 4913 4930 5083 5268 5713
Size: 1255 2073 229 1031 17 153 185 445
Cuts at: 5713 325
Size: 1498
Fragments arranged by size:
2073 1498 1255 1031 445 229 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3547 3649 5132
Size: 274 80 33 51 1443 1570 102 1483
Cuts at: 5132 6844 96
Size: 1712 138
Fragments arranged by size:
1712 1570 1483 1443 274 138 102 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6846 6878 6846
Size: 32 6854
Tth111I GACn'n_nGTC
Cuts at: 1316 2477 2765 1316
Size: 1161 288 5437
Fragments arranged by size:
5437 1161 288
XbaI T'CTAG_A
Cuts at: 4222 4222
Size: 6886
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3064 3247 3064
Size: 183 6703
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6886
XmaI C'CCGG_G
Cuts at: 1870 4211 1870
Size: 2341 4545
XmnI GAAnn'nnTTC
Cuts at: 2820 3294 5015 2820
Size: 474 1721 4691
Fragments arranged by size:
4691 1721 474
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4694 276
Size: 53 83 186 936 3160 2468
Fragments arranged by size:
3160 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI