Restriction map for
pFN21AE0046
(Circular) (Six-base) MAPSORT of: pFN21AE0046.seq Check: 4806 from: 1 to: 6622
pFN21AE0046 6622 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3540 3540
Size: 6622
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4432 278
Size: 53 83 186 936 2896 2468
Fragments arranged by size:
2896 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3965 1937
Size: 2028 4594
Acc65I G'GTAC_C
Cuts at: 2739 3943 2739
Size: 1204 5418
AclI AA'CG_TT
Cuts at: 1563 4749 5122 1563
Size: 3186 373 3063
Fragments arranged by size:
3186 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2051 3190 3478 4685 6069 6337 1632
Size: 419 1139 288 1207 1384 268 1917
Fragments arranged by size:
1917 1384 1207 1139 419 288 268
AfeI AGC'GCT
Cuts at: 3380 3380
Size: 6622
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6425
AflIII A'CryG_T
Cuts at: 1203 3759 5521 6498 1203
Size: 2556 1762 977 1327
Fragments arranged by size:
2556 1762 1327 977
AgeI A'CCGG_T
Cuts at: 5453 5453
Size: 6622
AhdI GACnn_n'nnGTC
Cuts at: 3124 5351 3124
Size: 2227 4395
AleI CACnn'nnGTG
Cuts at: 1166 3266 1166
Size: 2100 4522
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6622
AlwNI CAG_nnn'CTG
Cuts at: 2652 4365 5937 2652
Size: 1713 1572 3337
Fragments arranged by size:
3337 1713 1572
ApaI G_GGCC'C
Cuts at: 2236 3168 2236
Size: 932 5690
ApaLI G'TGCA_C
Cuts at: 4679 5835 4679
Size: 1156 5466
ApoI r'AATT_y
Cuts at: 1493 3931 4157 4309 6418 1493
Size: 2438 226 152 2109 1697
Fragments arranged by size:
2438 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5176 160
Size: 872 4144 1606
Fragments arranged by size:
4144 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6622
AvaI C'yCGr_G
Cuts at: 1870 1943 2229 3620 3947 1870
Size: 73 286 1391 327 4545
Fragments arranged by size:
4545 1391 327 286 73
BamHI G'GATC_C
Cuts at: 2901 3952 4319 2901
Size: 1051 367 5204
Fragments arranged by size:
5204 1051 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2628 2739 2851 3268 3560
Size: 325 217 627 841 111 112 417 292
Cuts at: 3560 3626 3719 3943 5398 618
Size: 66 93 224 1455 1842
Fragments arranged by size:
1842 1455 841 627 417 325 292 224
217 112 111 93 66
BanII G_rGCy'C
Cuts at: 1391 2236 2330 2806 3168 3465 3876 3941 1391
Size: 845 94 476 362 297 411 65 4072
Fragments arranged by size:
4072 845 476 411 362 297 94 65
BbeI G_GCGC'C
Cuts at: 3630 3630
Size: 6622
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2587 928
Size: 971 688 4963
Fragments arranged by size:
4963 971 688
BbvCI CC'TCA_GC
Cuts at: 2021 3169 2021
Size: 1148 5474
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4813 4847 1903
Size: 34 2876 34 3678
Fragments arranged by size:
3678 2876 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4514 5724 4514
Size: 1210 5412
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6622
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3639 5233 136
Size: 107 122 71 631 605 1967 1594 1525
Fragments arranged by size:
1967 1594 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2389 6617 2389
Size: 4228 2394
BlpI GC'TnA_GC
Cuts at: 4039 4039
Size: 6622
Bme1580I G_kGCm'C
Cuts at: 1790 2082 2236 3168 4683 5839 1790
Size: 292 154 932 1515 1156 2573
Fragments arranged by size:
2573 1515 1156 932 292 154
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6622
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2529 4353 5311
Size: 416 53 83 399 24 1112 1824 958
Cuts at: 5311 442
Size: 1753
Fragments arranged by size:
1824 1753 1112 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6622
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2941 2941
Size: 6622
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2483 2889 3668 3815 5282 1239
Size: 1244 406 779 147 1467 2579
Fragments arranged by size:
2579 1467 1244 779 406 147
Bpu10I CC'TnA_GC
Cuts at: 2021 3169 2021
Size: 1148 5474
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2685 4747 5612 5910 2685
Size: 2062 865 298 3397
Fragments arranged by size:
3397 2062 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5285 882
Size: 617 3786 2219
Fragments arranged by size:
3786 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4413 493
Size: 711 3209 2702
Fragments arranged by size:
3209 2702 711
BsaBI GATnn'nnATC
Cuts at: 2388 6616 2388
Size: 4228 2394
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2592 3627 3653 4429
Size: 53 83 186 936 1059 1035 26 776
Cuts at: 4429 4811 5446 275
Size: 382 635 1451
Fragments arranged by size:
1451 1059 1035 936 776 635 382 186
83 53 26
BsaWI w'CCGG_w
Cuts at: 2756 2898 5055 5453 5727 5874 6285 2756
Size: 142 2157 398 274 147 411 3093
Fragments arranged by size:
3093 2157 411 398 274 147 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3227 3257 3746 3776 1178
Size: 30 165 30 1824 30 489 30 4024
Fragments arranged by size:
4024 1824 489 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2814 3050 3443 1186
Size: 1628 236 393 4365
Fragments arranged by size:
4365 1628 393 236
BseYI C'CCAG_C
Cuts at: 3486 5825 6321 3486
Size: 2339 496 3787
Fragments arranged by size:
3787 2339 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2186 1163
Size: 573 450 5599
Fragments arranged by size:
5599 573 450
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3276 3826 4072 4833 5861 1540
Size: 275 186 1275 550 246 761 1028 2301
Fragments arranged by size:
2301 1275 1028 761 550 275 246 186
BsiHKAI G_wGCw'C
Cuts at: 2586 4683 4768 5839 2586
Size: 2097 85 1071 3369
Fragments arranged by size:
3369 2097 1071 85
BsmI GAATG_Cn'
Cuts at: 4133 4226 4133
Size: 93 6529
BsmBI CGTCTCn'nnnn_
Cuts at: 3644 3644
Size: 6622
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2236 2330 2586 2806 3168 3465
Size: 399 292 154 94 256 220 362 297
Cuts at: 3465 3876 3941 4683 4768 5839 1391
Size: 411 65 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 411 399 362 297 292
256 220 154 94 85 65
BspEI T'CCGG_A
Cuts at: 6285 6285
Size: 6622
BspHI T'CATG_A
Cuts at: 2380 4511 6241 2380
Size: 2131 1730 2761
Fragments arranged by size:
2761 2131 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3540 3840 3977 844
Size: 2696 300 137 3489
Fragments arranged by size:
3489 2696 300 137
BsrBI CCG'CTC
Cuts at: 1394 1823 4509 1394
Size: 429 2686 3507
Fragments arranged by size:
3507 2686 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5117 5291 65
Size: 1183 3869 174 1396
Fragments arranged by size:
3869 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3271 3731 5266 5453 1700
Size: 110 1461 460 1535 187 2869
Fragments arranged by size:
2869 1535 1461 460 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6622
BssHII G'CGCG_C
Cuts at: 1925 3503 3597 3669 1925
Size: 1578 94 72 4878
Fragments arranged by size:
4878 1578 94 72
BssSI C'ACGA_G
Cuts at: 3350 4682 5694 3350
Size: 1332 1012 4278
Fragments arranged by size:
4278 1332 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6622
BstBI TT'CG_AA
Cuts at: 5426 5426
Size: 6622
BstEII G'GTnAC_C
Cuts at: 1184 2894 1184
Size: 1710 4912
BstYI r'GATC_y
Cuts at: 1972 2389 2619 2901 3952 4319 4704 4721 6173
Size: 417 230 282 1051 367 385 17 1452
Cuts at: 6173 6259 6271 6617 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1051 417 385 367 346 282
230 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 3160 3174 3261 3282 3481
Size: 552 939 48 1108 14 87 21 199
Cuts at: 3481 3536 3634 3805 3820 513
Size: 55 98 171 15 3315
Fragments arranged by size:
3315 1108 939 552 199 171 98 87
55 48 21 15 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5883
Fragments arranged by size:
5883 683 56
BtsI GCAGTG_nn'
Cuts at: 2174 3154 4138 4932 4952 2174
Size: 980 984 794 20 3844
Fragments arranged by size:
3844 984 980 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6622
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2829 2864 529
Size: 35 2265 35 4287
Fragments arranged by size:
4287 2265 35 35
DraI TTT'AAA
Cuts at: 3926 3926
Size: 6622
DraIII CAC_nnn'GTG
Cuts at: 2858 2858
Size: 6622
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5629 809
Size: 507 4313 1802
Fragments arranged by size:
4313 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 3273 3316
Size: 54 1425 50 275 18 267 1176 43
Cuts at: 3316 3823 4069 4958 6587 8
Size: 507 246 889 1629 43
Fragments arranged by size:
1629 1425 1176 889 507 275 267 246
54 50 43 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3273 3823 4069 1537
Size: 275 1461 550 246 4090
Fragments arranged by size:
4090 1461 550 275 246
EarI CTCTTCn'nnn_
Cuts at: 1353 2042 2111 2606 3854 4552 1353
Size: 689 69 495 1248 698 3423
Fragments arranged by size:
3423 1248 698 689 495 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3041 5209 5583 5729 387
Size: 1064 138 1452 2168 374 146 1280
Fragments arranged by size:
2168 1452 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2483 2889 3190 3478 3668 3815
Size: 393 419 432 406 301 288 190 147
Cuts at: 3815 4685 5282 6069 6337 1239
Size: 870 597 787 268 1524
Fragments arranged by size:
1524 870 787 597 432 419 406 393
301 288 268 190 147
EcoNI CCTnn'n_nnAGG
Cuts at: 2796 3032 2796
Size: 236 6386
EcoO109I rG'GnC_Cy
Cuts at: 2266 2935 3165 2266
Size: 669 230 5723
Fragments arranged by size:
5723 669 230
EcoRI G'AATT_C
Cuts at: 3931 4309 3931
Size: 378 6244
FspI TGC'GCA
Cuts at: 5128 5128
Size: 6622
HaeII r_GCGC'y
Cuts at: 1678 3382 3610 3630 3693 5769 1678
Size: 1704 228 20 63 2076 2531
Fragments arranged by size:
2531 2076 1704 228 63 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3110 3142 3227 3259 3641
Size: 32 135 32 1538 32 85 32 382
Cuts at: 3641 3673 3746 3778 5263 5295 5337 5369 1373
Size: 32 73 32 1485 32 42 32 2626
Fragments arranged by size:
2626 1538 1485 382 135 85 73 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3966 4212 669
Size: 988 281 2028 246 3079
Fragments arranged by size:
3079 2028 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2405 748
Size: 1657 4965
HpaI GTT'AAC
Cuts at: 4212 4212
Size: 6622
KasI G'GCGC_C
Cuts at: 3626 3626
Size: 6622
KpnI G_GTAC'C
Cuts at: 2743 3947 2743
Size: 1204 5418
MfeI C'AATT_G
Cuts at: 4221 4221
Size: 6622
MluI A'CGCG_T
Cuts at: 3759 6498 3759
Size: 2739 3883
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3213 3789 3923 5736 5920 1305
Size: 1908 576 134 1813 184 2007
Fragments arranged by size:
2007 1908 1813 576 184 134
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2099 10
Size: 54 1425 343 267 4533
Fragments arranged by size:
4533 1425 343 267 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2822 3266 4580 4939 5098 518
Size: 648 1656 444 1314 359 159 2042
Fragments arranged by size:
2042 1656 1314 648 444 359 159
MspA1I CmG'CkG
Cuts at: 1606 2424 2649 2667 3162 3322 3402 3483 3538
Size: 818 225 18 495 160 80 81 55
Cuts at: 3538 3636 3807 3822 4038 4715 5863 6108 1606
Size: 98 171 15 216 677 1148 245 2120
Fragments arranged by size:
2120 1148 818 677 495 245 225 216
171 160 98 81 80 55 18 15
NaeI GCC'GGC
Cuts at: 3273 3733 3273
Size: 460 6162
NarI GG'CG_CC
Cuts at: 3627 3627
Size: 6622
NcoI C'CATG_G
Cuts at: 513 1065 2004 2052 3174 3282 513
Size: 552 939 48 1122 108 3853
Fragments arranged by size:
3853 1122 939 552 108 48
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6622
NgoMIV G'CCGG_C
Cuts at: 3271 3731 3271
Size: 460 6162
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6622
NotI GC'GGCC_GC
Cuts at: 3823 4069 3823
Size: 246 6376
NspI r_CATG'y
Cuts at: 1132 1229 2221 2821 3980 5525 1132
Size: 97 992 600 1159 1545 2229
Fragments arranged by size:
2229 1545 1159 992 600 97
PasI CC'CwG_GG
Cuts at: 1348 2341 1348
Size: 993 5629
PciI A'CATG_T
Cuts at: 5521 5521
Size: 6622
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6622
PmeI GTTT'AAAC
Cuts at: 3926 3926
Size: 6622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4691 4723 6204 6236 4691
Size: 32 1481 32 5077
Fragments arranged by size:
5077 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2266 2935 2266
Size: 669 5953
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6622
PsiI TTA'TAA
Cuts at: 4192 4192
Size: 6622
PspOMI G'GGCC_C
Cuts at: 2232 3164 2232
Size: 932 5690
PstI C_TGCA'G
Cuts at: 830 2161 3974 830
Size: 1331 1813 3478
Fragments arranged by size:
3478 1813 1331
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6622
PvuII CAG'CTG
Cuts at: 2649 2649
Size: 6622
SacII CC_GC'GG
Cuts at: 3163 3484 3539 3637 3808 3823 3163
Size: 321 55 98 171 15 5962
Fragments arranged by size:
5962 321 171 98 55 15
SalI G'TCGA_C
Cuts at: 1936 3964 1936
Size: 2028 4594
SanDI GG'GwC_CC
Cuts at: 2266 2266
Size: 6622
SapI GCTCTTCn'nnn_
Cuts at: 2111 2111
Size: 6622
SbfI CC_TGCA'GG
Cuts at: 3974 3974
Size: 6622
ScaI AGT'ACT
Cuts at: 3434 4870 6582 3434
Size: 1436 1712 3474
Fragments arranged by size:
3474 1712 1436
SfcI C'TryA_G
Cuts at: 826 1045 2157 2353 3784 3970 5105 5786 5977
Size: 219 1112 196 1431 186 1135 681 191
Cuts at: 5977 826
Size: 1471
Fragments arranged by size:
1471 1431 1135 1112 681 219 196 191
186
SfoI GGC'GCC
Cuts at: 3628 3628
Size: 6622
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6622
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6622
SmaI CCC'GGG
Cuts at: 1872 2231 3949 1872
Size: 359 1718 4545
Fragments arranged by size:
4545 1718 359
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2700 4726 5627 5889 820
Size: 197 926 757 2026 901 262 1553
Fragments arranged by size:
2026 1553 926 901 757 262 197
SnaBI TAC'GTA
Cuts at: 493 4413 493
Size: 3920 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6622
SphI G_CATG'C
Cuts at: 1132 2821 3980 1132
Size: 1689 1159 3774
Fragments arranged by size:
3774 1689 1159
SspI AAT'ATT
Cuts at: 5 52 4546 5
Size: 47 4494 2081
Fragments arranged by size:
4494 2081 47
StuI AGG'CCT
Cuts at: 1517 2064 1517
Size: 547 6075
StyI C'CwwG_G
Cuts at: 513 1065 2004 2052 2211 2847 3174 3282 3423
Size: 552 939 48 159 636 327 108 141
Cuts at: 3423 4061 513
Size: 638 3074
Fragments arranged by size:
3074 939 638 636 552 327 159 141
108 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2554 3485 3583 4649 4666 4819 5004
Size: 1255 974 931 98 1066 17 153 185
Cuts at: 5004 5449 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1066 974 931 445 185 153
98 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2940 3432 4868
Size: 274 80 33 51 1443 963 492 1436
Cuts at: 4868 6580 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1436 963 492 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6582 6614 6582
Size: 32 6590
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6622
XbaI T'CTAG_A
Cuts at: 3958 3958
Size: 6622
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2542 3289 2542
Size: 747 5875
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6622
XmaI C'CCGG_G
Cuts at: 1870 2229 3947 1870
Size: 359 1718 4545
Fragments arranged by size:
4545 1718 359
XmnI GAAnn'nnTTC
Cuts at: 2064 3254 4751 2064
Size: 1190 1497 3935
Fragments arranged by size:
3935 1497 1190
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4430 276
Size: 53 83 186 936 2896 2468
Fragments arranged by size:
2896 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I
Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacI SexAI
SfiI SrfI SwaI