Restriction map for
pFN21AE0045
(Circular) (Six-base) MAPSORT of: pFN21AE0045.seq Check: 4878 from: 1 to: 6688
pFN21AE0045 6688 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4498 278
Size: 53 83 186 936 2962 2468
Fragments arranged by size:
2962 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3624 4031 1937
Size: 1687 407 4594
Fragments arranged by size:
4594 1687 407
Acc65I G'GTAC_C
Cuts at: 2784 4009 2784
Size: 1225 5463
AclI AA'CG_TT
Cuts at: 1563 4815 5188 1563
Size: 3252 373 3063
Fragments arranged by size:
3252 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2346 3630 4751 6135 6403 1632
Size: 714 1284 1121 1384 268 1917
Fragments arranged by size:
1917 1384 1284 1121 714 268
AflII C'TTAA_G
Cuts at: 820 1017 3979 820
Size: 197 2962 3529
Fragments arranged by size:
3529 2962 197
AflIII A'CryG_T
Cuts at: 1203 2228 2459 5587 6564 1203
Size: 1025 231 3128 977 1327
Fragments arranged by size:
3128 1327 1025 977 231
AgeI A'CCGG_T
Cuts at: 5519 5519
Size: 6688
AhdI GACnn_n'nnGTC
Cuts at: 5417 5417
Size: 6688
AleI CACnn'nnGTG
Cuts at: 1166 3733 1166
Size: 2567 4121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6688
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2697 2729 2880 2912 2697
Size: 32 151 32 6473
Fragments arranged by size:
6473 151 32 32
AlwNI CAG_nnn'CTG
Cuts at: 4431 6003 4431
Size: 1572 5116
ApaLI G'TGCA_C
Cuts at: 3574 4745 5901 3574
Size: 1171 1156 4361
Fragments arranged by size:
4361 1171 1156
ApoI r'AATT_y
Cuts at: 1493 2331 2856 3046 3997 4223 4375 6484 1493
Size: 838 525 190 951 226 152 2109 1697
Fragments arranged by size:
2109 1697 951 838 525 226 190 152
AseI AT'TA_AT
Cuts at: 160 1032 2657 5242 160
Size: 872 1625 2585 1606
Fragments arranged by size:
2585 1625 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6688
AvaI C'yCGr_G
Cuts at: 1870 1943 4013 1870
Size: 73 2070 4545
Fragments arranged by size:
4545 2070 73
BamHI G'GATC_C
Cuts at: 3257 4018 4385 3257
Size: 761 367 5560
Fragments arranged by size:
5560 761 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2784 4009 5464 618
Size: 325 217 627 997 1225 1455 1842
Fragments arranged by size:
1842 1455 1225 997 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4007 1391
Size: 2616 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2215 2418 2582 928
Size: 971 316 203 164 5034
Fragments arranged by size:
5034 971 316 203 164
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4879 4913 1903
Size: 34 2942 34 3678
Fragments arranged by size:
3678 2942 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4580 5790 4580
Size: 1210 5478
BclI T'GATC_A
Cuts at: 1548 3667 1548
Size: 2119 4569
BfrBI ATG'CAT
Cuts at: 2985 3092 2985
Size: 107 6581
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5299 136
Size: 107 122 71 631 605 3627 1525
Fragments arranged by size:
3627 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6683 6683
Size: 6688
BlpI GC'TnA_GC
Cuts at: 4105 4105
Size: 6688
Bme1580I G_kGCm'C
Cuts at: 1790 3578 4749 5905 1790
Size: 1788 1171 1156 2573
Fragments arranged by size:
2573 1788 1171 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6688
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4419 5377 442
Size: 416 53 83 399 24 3002 958 1753
Fragments arranged by size:
3002 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2235 2400 2586 5348 1239
Size: 996 165 186 2762 2579
Fragments arranged by size:
2762 2579 996 186 165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4813 5678 5976 4813
Size: 865 298 5525
Fragments arranged by size:
5525 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2253 2616 5351 882
Size: 617 754 363 2735 2219
Fragments arranged by size:
2735 2219 754 617 363
BsaAI yAC'GTr
Cuts at: 493 1204 2229 4479 493
Size: 711 1025 2250 2702
Fragments arranged by size:
2702 2250 1025 711
BsaBI GATnn'nnATC
Cuts at: 3864 6682 3864
Size: 2818 3870
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4495 4877 5512 275
Size: 53 83 186 936 2962 382 635 1451
Fragments arranged by size:
2962 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5121 5519 5793 5940 6351 5121
Size: 398 274 147 411 5458
Fragments arranged by size:
5458 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2697 2727 2880 2910 2959
Size: 30 165 30 1294 30 153 30 49
Cuts at: 2959 2989 3145 3175 3568 3598 1178
Size: 30 156 30 393 30 4268
Fragments arranged by size:
4268 1294 393 165 156 153 49 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2597 2820 3540 3573 3576 3870 1186
Size: 1411 223 720 33 3 294 4004
Fragments arranged by size:
4004 1411 720 294 223 33 3
BseYI C'CCAG_C
Cuts at: 5891 6387 5891
Size: 496 6192
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3060 3533 1163
Size: 573 1324 473 4318
Fragments arranged by size:
4318 1324 573 473
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4138 4899 5927 1540
Size: 275 186 2137 761 1028 2301
Fragments arranged by size:
2301 2137 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2645 3578 4749 4834 5905 2645
Size: 933 1171 85 1071 3428
Fragments arranged by size:
3428 1171 1071 933 85
BsmI GAATG_Cn'
Cuts at: 3909 4199 4292 3909
Size: 290 93 6305
Fragments arranged by size:
6305 290 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2645 3578 4007 4749 4834 5905 1391
Size: 399 855 933 429 742 85 1071 2174
Fragments arranged by size:
2174 1071 933 855 742 429 399 85
BspEI T'CCGG_A
Cuts at: 6351 6351
Size: 6688
BspHI T'CATG_A
Cuts at: 2728 4577 6307 2728
Size: 1849 1730 3109
Fragments arranged by size:
3109 1849 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4043 844
Size: 3199 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4575 1394
Size: 429 2752 3507
Fragments arranged by size:
3507 2752 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3286 3914 5183 5357 65
Size: 1183 2038 628 1269 174 1396
Fragments arranged by size:
2038 1396 1269 1183 628 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5332 5519 1700
Size: 110 3522 187 2869
Fragments arranged by size:
3522 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2231 96
Size: 2135 4553
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6688
BssSI C'ACGA_G
Cuts at: 4748 5760 4748
Size: 1012 5676
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3248 794
Size: 2454 4234
BstBI TT'CG_AA
Cuts at: 5492 5492
Size: 6688
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6688
BstYI r'GATC_y
Cuts at: 1972 2750 2925 3257 3686 3890 4018 4385 4770
Size: 778 175 332 429 204 128 367 385
Cuts at: 4770 4787 6239 6325 6337 6683 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 778 429 385 367 346 332
204 175 128 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2929 3115 513
Size: 552 1864 186 4086
Fragments arranged by size:
4086 1864 552 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5949
Fragments arranged by size:
5949 683 56
BtsI GCAGTG_nn'
Cuts at: 3521 4204 4998 5018 3521
Size: 683 794 20 5191
Fragments arranged by size:
5191 794 683 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6688
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3804 3839 529
Size: 35 3240 35 3378
Fragments arranged by size:
3378 3240 35 35
DraI TTT'AAA
Cuts at: 2131 3992 2131
Size: 1861 4827
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5695 809
Size: 507 4379 1802
Fragments arranged by size:
4379 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4135 5024 6653
Size: 54 1425 50 275 18 2305 889 1629
Cuts at: 6653 8
Size: 43
Fragments arranged by size:
2305 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4135 1537
Size: 275 2323 4090
Fragments arranged by size:
4090 2323 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2061 2211 2650 3649 4618 1353
Size: 708 150 439 999 969 3423
Fragments arranged by size:
3423 999 969 708 439 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5275 5649 5795 387
Size: 1064 138 3686 374 146 1280
Fragments arranged by size:
3686 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2235 2346 2400 2586 3630 4751 5348
Size: 393 603 111 54 186 1044 1121 597
Cuts at: 5348 6135 6403 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1121 1044 787 603 597 393 268
186 111 54
EcoRI G'AATT_C
Cuts at: 3997 4375 3997
Size: 378 6310
FspI TGC'GCA
Cuts at: 5194 5194
Size: 6688
HaeII r_GCGC'y
Cuts at: 1678 5835 1678
Size: 4157 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2408 2440 2598 2630 2959
Size: 32 135 32 836 32 158 32 329
Cuts at: 2959 2991 3145 3177 5329 5361 5403 5435 1373
Size: 32 154 32 2152 32 42 32 2626
Fragments arranged by size:
2626 2152 836 329 158 154 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4032 4278 669
Size: 988 281 2094 246 3079
Fragments arranged by size:
3079 2094 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2134 748
Size: 1386 5302
HpaI GTT'AAC
Cuts at: 4278 4278
Size: 6688
KpnI G_GTAC'C
Cuts at: 2788 4013 2788
Size: 1225 5463
MfeI C'AATT_G
Cuts at: 4287 4287
Size: 6688
MluI A'CGCG_T
Cuts at: 6564 6564
Size: 6688
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5802 5986 1305
Size: 4497 184 2007
Fragments arranged by size:
4497 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4866
Fragments arranged by size:
4866 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2605 2623 3091 3404 3733 4646 5005
Size: 648 1439 18 468 313 329 913 359
Cuts at: 5005 5164 518
Size: 159 2042
Fragments arranged by size:
2042 1439 913 648 468 359 329 313
159 18
MspA1I CmG'CkG
Cuts at: 1606 2564 3872 4104 4781 5929 6174 1606
Size: 958 1308 232 677 1148 245 2120
Fragments arranged by size:
2120 1308 1148 958 677 245 232
NcoI C'CATG_G
Cuts at: 513 1065 2929 3115 513
Size: 552 1864 186 4086
Fragments arranged by size:
4086 1864 552 186
NdeI CA'TA_TG
Cuts at: 387 3088 387
Size: 2701 3987
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6688
NotI GC'GGCC_GC
Cuts at: 4135 4135
Size: 6688
NsiI A_TGCA'T
Cuts at: 2987 3094 2987
Size: 107 6581
NspI r_CATG'y
Cuts at: 1132 1229 2347 2463 3096 4046 5591 1132
Size: 97 1118 116 633 950 1545 2229
Fragments arranged by size:
2229 1545 1118 950 633 116 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6688
PciI A'CATG_T
Cuts at: 2459 5587 2459
Size: 3128 3560
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6688
PmeI GTTT'AAAC
Cuts at: 3992 3992
Size: 6688
PmlI CAC'GTG
Cuts at: 2229 2229
Size: 6688
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2697 2729 2880 2912 4757 4789 6270 6302 2697
Size: 32 151 32 1845 32 1481 32 3083
Fragments arranged by size:
3083 1845 1481 151 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6688
PsiI TTA'TAA
Cuts at: 4258 4258
Size: 6688
PstI C_TGCA'G
Cuts at: 830 4040 830
Size: 3210 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6688
PvuII CAG'CTG
Cuts at: 2564 3872 2564
Size: 1308 5380
SalI G'TCGA_C
Cuts at: 1936 4030 1936
Size: 2094 4594
SapI GCTCTTCn'nnn_
Cuts at: 2211 2650 3649 2211
Size: 439 999 5250
Fragments arranged by size:
5250 999 439
SbfI CC_TGCA'GG
Cuts at: 4040 4040
Size: 6688
ScaI AGT'ACT
Cuts at: 4936 6648 4936
Size: 1712 4976
SfcI C'TryA_G
Cuts at: 826 1045 2440 4036 5171 5852 6043 826
Size: 219 1395 1596 1135 681 191 1471
Fragments arranged by size:
1596 1471 1395 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6688
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6688
SmaI CCC'GGG
Cuts at: 1872 4015 1872
Size: 2143 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3979 4792 5693 5955 820
Size: 197 926 2036 813 901 262 1553
Fragments arranged by size:
2036 1553 926 901 813 262 197
SnaBI TAC'GTA
Cuts at: 493 4479 493
Size: 3986 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6688
SphI G_CATG'C
Cuts at: 1132 4046 1132
Size: 2914 3774
SspI AAT'ATT
Cuts at: 5 52 2117 3287 4612 5
Size: 47 2065 1170 1325 2081
Fragments arranged by size:
2081 2065 1325 1170 47
StuI AGG'CCT
Cuts at: 1517 3698 1517
Size: 2181 4507
StyI C'CwwG_G
Cuts at: 513 1065 2258 2929 3115 4127 513
Size: 552 1193 671 186 1012 3074
Fragments arranged by size:
3074 1193 1012 671 552 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4715 4732 4885 5070 5515 325
Size: 1255 3135 17 153 185 445 1498
Fragments arranged by size:
3135 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2231 2530 3365
Size: 274 80 33 51 1443 254 299 835
Cuts at: 3365 3548 4934 6646 96
Size: 183 1386 1712 138
Fragments arranged by size:
1712 1443 1386 835 299 274 254 183
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3249 3281 3568 3600 6648 6680 3249
Size: 32 287 32 3048 32 3257
Fragments arranged by size:
3257 3048 287 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6688
XbaI T'CTAG_A
Cuts at: 4024 4024
Size: 6688
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6688
XmaI C'CCGG_G
Cuts at: 1870 4013 1870
Size: 2143 4545
XmnI GAAnn'nnTTC
Cuts at: 2400 2491 2510 2895 4817 2400
Size: 91 19 385 1922 4271
Fragments arranged by size:
4271 1922 385 91 19
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4496 276
Size: 53 83 186 936 2962 2468
Fragments arranged by size:
2962 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI