Restriction map for
pFN21AE0043
(Circular) (Six-base) MAPSORT of: pFN21AE0043.seq Check: 9076 from: 1 to: 6337
pFN21AE0043 6337 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4147 278
Size: 53 83 186 936 2611 2468
Fragments arranged by size:
2611 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3680 1937
Size: 1743 4594
Acc65I G'GTAC_C
Cuts at: 3658 3658
Size: 6337
AclI AA'CG_TT
Cuts at: 1563 4464 4837 1563
Size: 2901 373 3063
Fragments arranged by size:
3063 2901 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2723 3390 3430 4400 5784 6052 1632
Size: 1091 667 40 970 1384 268 1917
Fragments arranged by size:
1917 1384 1091 970 667 268 40
AflII C'TTAA_G
Cuts at: 820 1017 2998 820
Size: 197 1981 4159
Fragments arranged by size:
4159 1981 197
AflIII A'CryG_T
Cuts at: 1203 2133 5236 6213 1203
Size: 930 3103 977 1327
Fragments arranged by size:
3103 1327 977 930
AgeI A'CCGG_T
Cuts at: 2819 5168 2819
Size: 2349 3988
AhdI GACnn_n'nnGTC
Cuts at: 5066 5066
Size: 6337
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6337
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6337
AlwNI CAG_nnn'CTG
Cuts at: 2219 2437 3322 4080 5652 2219
Size: 218 885 758 1572 2904
Fragments arranged by size:
2904 1572 885 758 218
ApaLI G'TGCA_C
Cuts at: 3511 4394 5550 3511
Size: 883 1156 4298
Fragments arranged by size:
4298 1156 883
ApoI r'AATT_y
Cuts at: 1493 2376 2423 2792 2890 3428 3440 3498 3646
Size: 883 47 369 98 538 12 58 148
Cuts at: 3646 3872 4024 6133 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 883 538 369 226 152 148
98 58 47 12
AseI AT'TA_AT
Cuts at: 160 1032 3578 4891 160
Size: 872 2546 1313 1606
Fragments arranged by size:
2546 1606 1313 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6337
AvaI C'yCGr_G
Cuts at: 1870 1943 2257 3662 1870
Size: 73 314 1405 4545
Fragments arranged by size:
4545 1405 314 73
BamHI G'GATC_C
Cuts at: 3667 4034 3667
Size: 367 5970
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3658 5113 618
Size: 325 217 627 1871 1455 1842
Fragments arranged by size:
1871 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2317 3656 1391
Size: 926 1339 4072
Fragments arranged by size:
4072 1339 926
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2063 2176 2336 2862 3328 928
Size: 971 164 113 160 526 466 3937
Fragments arranged by size:
3937 971 526 466 164 160 113
BbvCI CC'TCA_GC
Cuts at: 2609 2609
Size: 6337
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4528 4562 1903
Size: 34 2591 34 3678
Fragments arranged by size:
3678 2591 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4229 5439 4229
Size: 1210 5127
BclI T'GATC_A
Cuts at: 1548 3609 1548
Size: 2061 4276
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4948 136
Size: 107 122 71 631 605 3276 1525
Fragments arranged by size:
3276 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2067 3562 6332 2067
Size: 1495 2770 2072
Fragments arranged by size:
2770 2072 1495
BlpI GC'TnA_GC
Cuts at: 3754 3754
Size: 6337
Bme1580I G_kGCm'C
Cuts at: 1790 2079 2595 2749 3515 4398 5554 1790
Size: 289 516 154 766 883 1156 2573
Fragments arranged by size:
2573 1156 883 766 516 289 154
BmgBI CAC'GTC
Cuts at: 1318 2134 1318
Size: 816 5521
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2348 2635 2910
Size: 416 53 83 399 24 931 287 275
Cuts at: 2910 4068 5026 442
Size: 1158 958 1753
Fragments arranged by size:
1753 1158 958 931 416 399 287 275
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 3569 4997 1239
Size: 845 1485 1428 2579
Fragments arranged by size:
2579 1485 1428 845
Bpu10I CC'TnA_GC
Cuts at: 2609 2609
Size: 6337
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4462 5327 5625 4462
Size: 865 298 5174
Fragments arranged by size:
5174 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5000 882
Size: 617 3501 2219
Fragments arranged by size:
3501 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4128 493
Size: 711 2924 2702
Fragments arranged by size:
2924 2702 711
BsaBI GATnn'nnATC
Cuts at: 6331 6331
Size: 6337
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4144 4526 5161 275
Size: 53 83 186 936 2611 382 635 1451
Fragments arranged by size:
2611 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2819 4770 5168 5442 5589 6000 2819
Size: 1951 398 274 147 411 3156
Fragments arranged by size:
3156 1951 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2925 2955 1178
Size: 30 165 30 1522 30 4560
Fragments arranged by size:
4560 1522 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6337
BseYI C'CCAG_C
Cuts at: 2445 5540 6036 2445
Size: 3095 496 2746
Fragments arranged by size:
3095 2746 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5764
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3787 4548 5576 1540
Size: 275 186 1786 761 1028 2301
Fragments arranged by size:
2301 1786 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3515 4398 4483 5554 3515
Size: 883 85 1071 4298
Fragments arranged by size:
4298 1071 883 85
BsmI GAATG_Cn'
Cuts at: 3848 3941 3848
Size: 93 6244
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2317 2595 2749 3515 3656 4398
Size: 399 289 238 278 154 766 141 742
Cuts at: 4398 4483 5554 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 766 742 399 289 278 238
154 141 85
BspEI T'CCGG_A
Cuts at: 6000 6000
Size: 6337
BspHI T'CATG_A
Cuts at: 4226 5956 4226
Size: 1730 4607
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3692 844
Size: 2848 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4224 1394
Size: 429 2401 3507
Fragments arranged by size:
3507 2401 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4832 5006 65
Size: 1183 3584 174 1396
Fragments arranged by size:
3584 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2819 4981 5168 1700
Size: 110 1009 2162 187 2869
Fragments arranged by size:
2869 2162 1009 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6337
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6337
BssSI C'ACGA_G
Cuts at: 2588 4397 5409 2588
Size: 1809 1012 3516
Fragments arranged by size:
3516 1809 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6337
BstBI TT'CG_AA
Cuts at: 5141 5141
Size: 6337
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6337
BstXI CCAn_nnnn'nTGG
Cuts at: 2305 2305
Size: 6337
BstYI r'GATC_y
Cuts at: 1972 2067 2496 2664 3562 3667 4034 4419 4436
Size: 95 429 168 898 105 367 385 17
Cuts at: 4436 5888 5974 5986 6332 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 898 429 385 367 346 168
105 95 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2324 2324
Size: 6337
BtgI C'CryG_G
Cuts at: 513 1065 2004 2298 513
Size: 552 939 294 4552
Fragments arranged by size:
4552 939 552 294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5598
Fragments arranged by size:
5598 683 56
BtsI GCAGTG_nn'
Cuts at: 2622 3853 4647 4667 2622
Size: 1231 794 20 4292
Fragments arranged by size:
4292 1231 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6337
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6302
DraI TTT'AAA
Cuts at: 3641 3641
Size: 6337
DraIII CAC_nnn'GTG
Cuts at: 3164 3164
Size: 6337
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5344 809
Size: 507 4028 1802
Fragments arranged by size:
4028 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3784 4673 6302
Size: 54 1425 50 275 18 1954 889 1629
Cuts at: 6302 8
Size: 43
Fragments arranged by size:
1954 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3784 1537
Size: 275 1972 4090
Fragments arranged by size:
4090 1972 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2239 2570 3471 4267 1353
Size: 886 331 901 796 3423
Fragments arranged by size:
3423 901 886 796 331
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4924 5298 5444 387
Size: 1064 138 3335 374 146 1280
Fragments arranged by size:
3335 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2723 3390 3430 3569 4400 4997
Size: 393 452 639 667 40 139 831 597
Cuts at: 4997 5784 6052 1239
Size: 787 268 1524
Fragments arranged by size:
1524 831 787 667 639 597 452 393
268 139 40
EcoO109I rG'GnC_Cy
Cuts at: 2254 3319 2254
Size: 1065 5272
EcoRI G'AATT_C
Cuts at: 2376 3440 3646 4024 2376
Size: 1064 206 378 4689
Fragments arranged by size:
4689 1064 378 206
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 6337
FspI TGC'GCA
Cuts at: 4843 4843
Size: 6337
HaeII r_GCGC'y
Cuts at: 1678 2124 5484 1678
Size: 446 3360 2531
Fragments arranged by size:
3360 2531 446
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2216 2248 3158 3190 4978
Size: 32 135 32 644 32 910 32 1788
Cuts at: 4978 5010 5052 5084 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1788 910 644 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2560 3681 3927 669
Size: 988 281 622 1121 246 3079
Fragments arranged by size:
3079 1121 988 622 281 246
HindIII A'AGCT_T
Cuts at: 748 2012 2802 3035 748
Size: 1264 790 233 4050
Fragments arranged by size:
4050 1264 790 233
HpaI GTT'AAC
Cuts at: 3927 3927
Size: 6337
KpnI G_GTAC'C
Cuts at: 3662 3662
Size: 6337
MfeI C'AATT_G
Cuts at: 3936 3936
Size: 6337
MluI A'CGCG_T
Cuts at: 6213 6213
Size: 6337
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2700 5451 5635 1305
Size: 1395 2751 184 2007
Fragments arranged by size:
2751 2007 1395 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4515
Fragments arranged by size:
4515 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2303 2366 4295 4654 4813 518
Size: 648 1137 63 1929 359 159 2042
Fragments arranged by size:
2042 1929 1137 648 359 159 63
MspA1I CmG'CkG
Cuts at: 1606 2219 2661 3753 4430 5578 5823 1606
Size: 613 442 1092 677 1148 245 2120
Fragments arranged by size:
2120 1148 1092 677 613 442 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2298 513
Size: 552 939 294 4552
Fragments arranged by size:
4552 939 552 294
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6337
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6337
NotI GC'GGCC_GC
Cuts at: 3784 3784
Size: 6337
NspI r_CATG'y
Cuts at: 1132 1229 3695 5240 1132
Size: 97 2466 1545 2229
Fragments arranged by size:
2466 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6337
PciI A'CATG_T
Cuts at: 5236 5236
Size: 6337
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2437 1108
Size: 1329 5008
PfoI T'CCnGG_A
Cuts at: 2943 2943
Size: 6337
PmeI GTTT'AAAC
Cuts at: 3641 3641
Size: 6337
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4406 4438 5919 5951 4406
Size: 32 1481 32 4792
Fragments arranged by size:
4792 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2254 2254
Size: 6337
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6337
PsiI TTA'TAA
Cuts at: 3907 3907
Size: 6337
PstI C_TGCA'G
Cuts at: 830 2224 3689 830
Size: 1394 1465 3478
Fragments arranged by size:
3478 1465 1394
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6337
PvuII CAG'CTG
Cuts at: 2219 2661 2219
Size: 442 5895
SalI G'TCGA_C
Cuts at: 1936 3679 1936
Size: 1743 4594
SapI GCTCTTCn'nnn_
Cuts at: 3471 3471
Size: 6337
SbfI CC_TGCA'GG
Cuts at: 3689 3689
Size: 6337
ScaI AGT'ACT
Cuts at: 4585 6297 4585
Size: 1712 4625
SexAI A'CCwGG_T
Cuts at: 2250 2404 2436 2250
Size: 154 32 6151
Fragments arranged by size:
6151 154 32
SfcI C'TryA_G
Cuts at: 826 1045 2220 3685 4820 5501 5692 826
Size: 219 1175 1465 1135 681 191 1471
Fragments arranged by size:
1471 1465 1175 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6337
SgrAI Cr'CCGG_yG
Cuts at: 1700 2819 1700
Size: 1119 5218
SmaI CCC'GGG
Cuts at: 1872 2259 3664 1872
Size: 387 1405 4545
Fragments arranged by size:
4545 1405 387
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2998 4441 5342 5604 820
Size: 197 926 1055 1443 901 262 1553
Fragments arranged by size:
1553 1443 1055 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4128 493
Size: 3635 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6337
SphI G_CATG'C
Cuts at: 1132 3695 1132
Size: 2563 3774
SspI AAT'ATT
Cuts at: 5 52 3058 3582 4261 5
Size: 47 3006 524 679 2081
Fragments arranged by size:
3006 2081 679 524 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6337
StyI C'CwwG_G
Cuts at: 513 1065 2004 2298 2575 3776 513
Size: 552 939 294 277 1201 3074
Fragments arranged by size:
3074 1201 939 552 294 277
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2325 2617 2929 2996 4364 4381 4534
Size: 1255 745 292 312 67 1368 17 153
Cuts at: 4534 4719 5164 325
Size: 185 445 1498
Fragments arranged by size:
1498 1368 1255 745 445 312 292 185
153 67 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2140 2335 4583
Size: 274 80 33 51 1443 163 195 2248
Cuts at: 4583 6295 96
Size: 1712 138
Fragments arranged by size:
2248 1712 1443 274 195 163 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2286 2318 2615 2647 6297 6329 2286
Size: 32 297 32 3650 32 2294
Fragments arranged by size:
3650 2294 297 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2252 3347 1316
Size: 936 1095 4306
Fragments arranged by size:
4306 1095 936
XbaI T'CTAG_A
Cuts at: 3673 3673
Size: 6337
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2501 2501
Size: 6337
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6337
XmaI C'CCGG_G
Cuts at: 1870 2257 3662 1870
Size: 387 1405 4545
Fragments arranged by size:
4545 1405 387
XmnI GAAnn'nnTTC
Cuts at: 2013 3480 4466 2013
Size: 1467 986 3884
Fragments arranged by size:
3884 1467 986
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4145 276
Size: 53 83 186 936 2611 2468
Fragments arranged by size:
2611 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI