Restriction map for
pFN21AE0042
(Circular) (Six-base) MAPSORT of: pFN21AE0042.seq Check: 612 from: 1 to: 6238
pFN21AE0042 6238 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4048 278
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3581 1937
Size: 1644 4594
Acc65I G'GTAC_C
Cuts at: 3559 3559
Size: 6238
AclI AA'CG_TT
Cuts at: 1563 4365 4738 1563
Size: 2802 373 3063
Fragments arranged by size:
3063 2802 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2294 4301 5685 5953 1632
Size: 662 2007 1384 268 1917
Fragments arranged by size:
2007 1917 1384 662 268
AflII C'TTAA_G
Cuts at: 820 1017 3398 820
Size: 197 2381 3660
Fragments arranged by size:
3660 2381 197
AflIII A'CryG_T
Cuts at: 1203 5137 6114 1203
Size: 3934 977 1327
Fragments arranged by size:
3934 1327 977
AgeI A'CCGG_T
Cuts at: 2404 5069 2404
Size: 2665 3573
AhdI GACnn_n'nnGTC
Cuts at: 4967 4967
Size: 6238
AleI CACnn'nnGTG
Cuts at: 1166 2405 1166
Size: 1239 4999
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6238
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2312 2344 2312
Size: 32 6206
AlwNI CAG_nnn'CTG
Cuts at: 2698 2887 3445 3981 5553 2698
Size: 189 558 536 1572 3383
Fragments arranged by size:
3383 1572 558 536 189
ApaLI G'TGCA_C
Cuts at: 4295 5451 4295
Size: 1156 5082
ApoI r'AATT_y
Cuts at: 1493 3547 3773 3925 6034 1493
Size: 2054 226 152 2109 1697
Fragments arranged by size:
2109 2054 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4792 160
Size: 872 3760 1606
Fragments arranged by size:
3760 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
AvaI C'yCGr_G
Cuts at: 1870 1943 2676 3563 1870
Size: 73 733 887 4545
Fragments arranged by size:
4545 887 733 73
BamHI G'GATC_C
Cuts at: 3568 3935 3568
Size: 367 5871
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2407 3559 5014 618
Size: 325 217 627 620 1152 1455 1842
Fragments arranged by size:
1842 1455 1152 627 620 325 217
BanII G_rGCy'C
Cuts at: 1391 2265 3378 3423 3453 3557 1391
Size: 874 1113 45 30 104 4072
Fragments arranged by size:
4072 1113 874 104 45 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2183 2907 928
Size: 971 284 724 4259
Fragments arranged by size:
4259 971 724 284
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3266 3300 4429 4463 1903
Size: 34 1329 34 1129 34 3678
Fragments arranged by size:
3678 1329 1129 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4130 5340 4130
Size: 1210 5028
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6238
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4849 136
Size: 107 122 71 631 605 3177 1525
Fragments arranged by size:
3177 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2550 6233 2550
Size: 3683 2555
BlpI GC'TnA_GC
Cuts at: 3655 3655
Size: 6238
Bme1580I G_kGCm'C
Cuts at: 1790 2301 2412 4299 5455 1790
Size: 511 111 1887 1156 2573
Fragments arranged by size:
2573 1887 1156 511 111
BmgBI CAC'GTC
Cuts at: 1318 2491 1318
Size: 1173 5065
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3969 4927 442
Size: 416 53 83 399 24 2552 958 1753
Fragments arranged by size:
2552 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6238
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2206 2206
Size: 6238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3053 3362 3467 4898 1239
Size: 1814 309 105 1431 2579
Fragments arranged by size:
2579 1814 1431 309 105
Bpu10I CC'TnA_GC
Cuts at: 3080 3417 3080
Size: 337 5901
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4363 5228 5526 4363
Size: 865 298 5075
Fragments arranged by size:
5075 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2196 2227 2633 3223 4901 882
Size: 617 697 31 406 590 1678 2219
Fragments arranged by size:
2219 1678 697 617 590 406 31
BsaAI yAC'GTr
Cuts at: 493 1204 2665 4029 493
Size: 711 1461 1364 2702
Fragments arranged by size:
2702 1461 1364 711
BsaBI GATnn'nnATC
Cuts at: 6232 6232
Size: 6238
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3126 4045 4427 5062
Size: 53 83 186 936 1593 919 382 635
Cuts at: 5062 275
Size: 1451
Fragments arranged by size:
1593 1451 936 919 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2236 2404 4671 5069 5343 5490 5901 2236
Size: 168 2267 398 274 147 411 2573
Fragments arranged by size:
2573 2267 411 398 274 168 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3435 3465 1178
Size: 30 165 30 2032 30 3951
Fragments arranged by size:
3951 2032 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2233 2696 2862 3269 1186
Size: 1047 463 166 407 4155
Fragments arranged by size:
4155 1047 463 407 166
BseYI C'CCAG_C
Cuts at: 5441 5937 5441
Size: 496 5742
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2761 2789 3185 1163
Size: 573 1025 28 396 4216
Fragments arranged by size:
4216 1025 573 396 28
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3688 4449 5477 1540
Size: 275 186 1687 761 1028 2301
Fragments arranged by size:
2301 1687 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3086 3378 3453 4299 4384 5455 3086
Size: 292 75 846 85 1071 3869
Fragments arranged by size:
3869 1071 846 292 85 75
BsmI GAATG_Cn'
Cuts at: 2369 3749 3842 2369
Size: 1380 93 4765
Fragments arranged by size:
4765 1380 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2265 2301 2412 3086 3378 3423 3453
Size: 399 475 36 111 674 292 45 30
Cuts at: 3453 3557 4299 4384 5455 1391
Size: 104 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 674 475 399 292 111
104 85 45 36 30
BspEI T'CCGG_A
Cuts at: 5901 5901
Size: 6238
BspHI T'CATG_A
Cuts at: 3313 4127 5857 3313
Size: 814 1730 3694
Fragments arranged by size:
3694 1730 814
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2706 2872 3464 3593 844
Size: 1862 166 592 129 3489
Fragments arranged by size:
3489 1862 592 166 129
BsrBI CCG'CTC
Cuts at: 1394 1823 4125 1394
Size: 429 2302 3507
Fragments arranged by size:
3507 2302 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4733 4907 65
Size: 1183 3485 174 1396
Fragments arranged by size:
3485 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2404 4882 5069 1700
Size: 110 594 2478 187 2869
Fragments arranged by size:
2869 2478 594 187 110
BsrGI T'GTAC_A
Cuts at: 96 2980 96
Size: 2884 3354
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6238
BssSI C'ACGA_G
Cuts at: 4298 5310 4298
Size: 1012 5226
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2698 794
Size: 1904 4334
BstBI TT'CG_AA
Cuts at: 5042 5042
Size: 6238
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6238
BstXI CCAn_nnnn'nTGG
Cuts at: 2213 2213
Size: 6238
BstYI r'GATC_y
Cuts at: 1972 2550 3568 3935 4320 4337 5789 5875 5887
Size: 578 1018 367 385 17 1452 86 12
Cuts at: 5887 6233 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1018 578 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2906 2906
Size: 6238
BtgI C'CryG_G
Cuts at: 513 1065 2151 2250 2374 2412 3157 513
Size: 552 1086 99 124 38 745 3594
Fragments arranged by size:
3594 1086 745 552 124 99 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2509 487
Size: 683 56 1283 4216
Fragments arranged by size:
4216 1283 683 56
BtsI GCAGTG_nn'
Cuts at: 3526 3754 4548 4568 3526
Size: 228 794 20 5196
Fragments arranged by size:
5196 794 228 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6238
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6203
DraI TTT'AAA
Cuts at: 3542 3542
Size: 6238
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3203 5245 809
Size: 507 1887 2042 1802
Fragments arranged by size:
2042 1887 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2215 2247 2841
Size: 54 1425 50 275 18 385 32 594
Cuts at: 2841 3685 4574 6203 8
Size: 844 889 1629 43
Fragments arranged by size:
1629 1425 889 844 594 385 275 54
50 43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 3685 1537
Size: 275 1873 4090
Fragments arranged by size:
4090 1873 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2084 2849 3246 4168 1353
Size: 731 765 397 922 3423
Fragments arranged by size:
3423 922 765 731 397
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4825 5199 5345 387
Size: 1064 138 3236 374 146 1280
Fragments arranged by size:
3236 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3376 3451 3376
Size: 75 6163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 3053 3362 3467 4301 4898 5685
Size: 393 662 759 309 105 834 597 787
Cuts at: 5685 5953 1239
Size: 268 1524
Fragments arranged by size:
1524 834 787 759 662 597 393 309
268 105
EcoNI CCTnn'n_nnAGG
Cuts at: 2227 2227
Size: 6238
EcoO109I rG'GnC_Cy
Cuts at: 2912 3205 2912
Size: 293 5945
EcoRI G'AATT_C
Cuts at: 3547 3925 3547
Size: 378 5860
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2146 2980 2146
Size: 834 5404
FspI TGC'GCA
Cuts at: 4744 4744
Size: 6238
HaeII r_GCGC'y
Cuts at: 1678 2438 2803 5385 1678
Size: 760 365 2582 2531
Fragments arranged by size:
2582 2531 760 365
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3248 3280 4879 4911 4953
Size: 32 135 32 1676 32 1599 32 42
Cuts at: 4953 4985 1373
Size: 32 2626
Fragments arranged by size:
2626 1676 1599 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3582 3828 669
Size: 988 281 1644 246 3079
Fragments arranged by size:
3079 1644 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2305 748
Size: 1557 4681
HpaI GTT'AAC
Cuts at: 3828 3828
Size: 6238
KpnI G_GTAC'C
Cuts at: 3563 3563
Size: 6238
MfeI C'AATT_G
Cuts at: 2025 3236 3837 2025
Size: 1211 601 4426
Fragments arranged by size:
4426 1211 601
MluI A'CGCG_T
Cuts at: 6114 6114
Size: 6238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3483 5352 5536 1305
Size: 2178 1869 184 2007
Fragments arranged by size:
2178 2007 1869 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2217 2843 10
Size: 54 1425 343 385 626 3405
Fragments arranged by size:
3405 1425 626 385 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2405 2459 4196 4555 4714 518
Size: 648 1239 54 1737 359 159 2042
Fragments arranged by size:
2042 1737 1239 648 359 159 54
MspA1I CmG'CkG
Cuts at: 1606 2573 3654 4331 5479 5724 1606
Size: 967 1081 677 1148 245 2120
Fragments arranged by size:
2120 1148 1081 967 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2250 2374 3157 513
Size: 552 1185 124 783 3594
Fragments arranged by size:
3594 1185 783 552 124
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6238
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6238
NotI GC'GGCC_GC
Cuts at: 3685 3685
Size: 6238
NspI r_CATG'y
Cuts at: 1132 1229 2815 3397 3596 5141 1132
Size: 97 1586 582 199 1545 2229
Fragments arranged by size:
2229 1586 1545 582 199 97
PasI CC'CwG_GG
Cuts at: 1348 3184 3211 1348
Size: 1836 27 4375
Fragments arranged by size:
4375 1836 27
PciI A'CATG_T
Cuts at: 5137 5137
Size: 6238
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2317 2375 3073 1108
Size: 1209 58 698 4273
Fragments arranged by size:
4273 1209 698 58
PmeI GTTT'AAAC
Cuts at: 3542 3542
Size: 6238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4307 4339 5820 5852 4307
Size: 32 1481 32 4693
Fragments arranged by size:
4693 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2912 3205 2912
Size: 293 5945
PshAI GACnn'nnGTC
Cuts at: 1613 2183 1613
Size: 570 5668
PsiI TTA'TAA
Cuts at: 3808 3808
Size: 6238
PstI C_TGCA'G
Cuts at: 830 3475 3590 830
Size: 2645 115 3478
Fragments arranged by size:
3478 2645 115
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6238
PvuII CAG'CTG
Cuts at: 2573 2573
Size: 6238
SacI G_AGCT'C
Cuts at: 3378 3453 3378
Size: 75 6163
SalI G'TCGA_C
Cuts at: 1936 3580 1936
Size: 1644 4594
SanDI GG'GwC_CC
Cuts at: 2912 2912
Size: 6238
SbfI CC_TGCA'GG
Cuts at: 3475 3590 3475
Size: 115 6123
ScaI AGT'ACT
Cuts at: 2351 4486 6198 2351
Size: 2135 1712 2391
Fragments arranged by size:
2391 2135 1712
SfcI C'TryA_G
Cuts at: 826 1045 3130 3471 3586 4721 5402 5593 826
Size: 219 2085 341 115 1135 681 191 1471
Fragments arranged by size:
2085 1471 1135 681 341 219 191 115
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
SgrAI Cr'CCGG_yG
Cuts at: 1700 2404 1700
Size: 704 5534
SmaI CCC'GGG
Cuts at: 1872 2678 3565 1872
Size: 806 887 4545
Fragments arranged by size:
4545 887 806
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3398 4342 5243 5505 820
Size: 197 926 1455 944 901 262 1553
Fragments arranged by size:
1553 1455 944 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4029 493
Size: 3536 2702
SpeI A'CTAG_T
Cuts at: 152 2474 152
Size: 2322 3916
SphI G_CATG'C
Cuts at: 1132 3596 1132
Size: 2464 3774
SspI AAT'ATT
Cuts at: 5 52 4162 5
Size: 47 4110 2081
Fragments arranged by size:
4110 2081 47
StuI AGG'CCT
Cuts at: 1517 3038 3293 1517
Size: 1521 255 4462
Fragments arranged by size:
4462 1521 255
StyI C'CwwG_G
Cuts at: 513 1065 2250 2374 2517 3157 3222 3677 513
Size: 552 1185 124 143 640 65 455 3074
Fragments arranged by size:
3074 1185 640 552 455 143 124 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2126 2573 4265 4282 4435 4620 5065
Size: 1255 546 447 1692 17 153 185 445
Cuts at: 5065 325
Size: 1498
Fragments arranged by size:
1692 1498 1255 546 447 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2349 2980 4484
Size: 274 80 33 51 1443 372 631 1504
Cuts at: 4484 6196 96
Size: 1712 138
Fragments arranged by size:
1712 1504 1443 631 372 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2064 2096 2798 2830 6198 6230 2064
Size: 32 702 32 3368 32 2072
Fragments arranged by size:
3368 2072 702 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6238
XbaI T'CTAG_A
Cuts at: 3574 3574
Size: 6238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2149 2011
Size: 138 6100
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6238
XmaI C'CCGG_G
Cuts at: 1870 2676 3563 1870
Size: 806 887 4545
Fragments arranged by size:
4545 887 806
XmnI GAAnn'nnTTC
Cuts at: 2826 4367 2826
Size: 1541 4697
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4046 276
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI
SfiI SfoI SrfI SwaI