Restriction map for
pFN21AE0040
(Circular) (Six-base) MAPSORT of: pFN21AE0040.seq Check: 4880 from: 1 to: 6613
pFN21AE0040 6613 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3036 3036
Size: 6613
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4423 278
Size: 53 83 186 936 2887 2468
Fragments arranged by size:
2887 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3956 1937
Size: 2019 4594
Acc65I G'GTAC_C
Cuts at: 3934 3934
Size: 6613
AclI AA'CG_TT
Cuts at: 1563 4740 5113 1563
Size: 3177 373 3063
Fragments arranged by size:
3177 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2489 3513 4676 6060 6328 1632
Size: 857 1024 1163 1384 268 1917
Fragments arranged by size:
1917 1384 1163 1024 857 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6416
AflIII A'CryG_T
Cuts at: 1203 3822 5512 6489 1203
Size: 2619 1690 977 1327
Fragments arranged by size:
2619 1690 1327 977
AgeI A'CCGG_T
Cuts at: 3550 5444 3550
Size: 1894 4719
AhdI GACnn_n'nnGTC
Cuts at: 3885 5342 3885
Size: 1457 5156
AleI CACnn'nnGTG
Cuts at: 1166 2549 3767 1166
Size: 1383 1218 4012
Fragments arranged by size:
4012 1383 1218
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2972 1184
Size: 1788 4825
AlwNI CAG_nnn'CTG
Cuts at: 2152 2437 3055 3322 4356 5928 2152
Size: 285 618 267 1034 1572 2837
Fragments arranged by size:
2837 1572 1034 618 285 267
ApaI G_GGCC'C
Cuts at: 2099 3761 2099
Size: 1662 4951
ApaLI G'TGCA_C
Cuts at: 4670 5826 4670
Size: 1156 5457
ApoI r'AATT_y
Cuts at: 1493 3922 4148 4300 6409 1493
Size: 2429 226 152 2109 1697
Fragments arranged by size:
2429 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5167 160
Size: 872 4135 1606
Fragments arranged by size:
4135 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6613
AvaI C'yCGr_G
Cuts at: 1870 1943 2270 3753 3938 1870
Size: 73 327 1483 185 4545
Fragments arranged by size:
4545 1483 327 185 73
BamHI G'GATC_C
Cuts at: 3943 4310 3943
Size: 367 6246
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2551 2845 3934 5389 618
Size: 325 217 627 764 294 1089 1455 1842
Fragments arranged by size:
1842 1455 1089 764 627 325 294 217
BanII G_rGCy'C
Cuts at: 1391 2099 3754 3761 3932 1391
Size: 708 1655 7 171 4072
Fragments arranged by size:
4072 1655 708 171 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5642
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3829 3863 4804 4838 1903
Size: 34 1892 34 941 34 3678
Fragments arranged by size:
3678 1892 941 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3022 4505 5715 3022
Size: 1483 1210 3920
Fragments arranged by size:
3920 1483 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6613
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3617 5224 136
Size: 107 122 71 631 605 1945 1607 1525
Fragments arranged by size:
1945 1607 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6608 6608
Size: 6613
BlpI GC'TnA_GC
Cuts at: 4030 4030
Size: 6613
Bme1580I G_kGCm'C
Cuts at: 1790 2099 3761 4674 5830 1790
Size: 309 1662 913 1156 2573
Fragments arranged by size:
2573 1662 1156 913 309
BmgBI CAC'GTC
Cuts at: 1318 2407 1318
Size: 1089 5524
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4344 5302 442
Size: 416 53 83 399 24 2927 958 1753
Fragments arranged by size:
2927 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6613
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2414 2858 3238 3642 3722 5273 1239
Size: 1175 444 380 404 80 1551 2579
Fragments arranged by size:
2579 1551 1175 444 404 380 80
Bpu10I CC'TnA_GC
Cuts at: 3389 3680 3389
Size: 291 6322
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2966 3569 4738 5603 5901 2966
Size: 603 1169 865 298 3678
Fragments arranged by size:
3678 1169 865 603 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2522 5276 882
Size: 617 1023 2754 2219
Fragments arranged by size:
2754 2219 1023 617
BsaAI yAC'GTr
Cuts at: 493 1204 3673 4404 493
Size: 711 2469 731 2702
Fragments arranged by size:
2702 2469 731 711
BsaBI GATnn'nnATC
Cuts at: 3149 6607 3149
Size: 3458 3155
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4420 4802 5437 275
Size: 53 83 186 936 2887 382 635 1451
Fragments arranged by size:
2887 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3550 5046 5444 5718 5865 6276 3550
Size: 1496 398 274 147 411 3887
Fragments arranged by size:
3887 1496 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6388
Fragments arranged by size:
6388 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 2333 2867 3294 3872 1186
Size: 1102 45 534 427 578 3927
Fragments arranged by size:
3927 1102 578 534 427 45
BseYI C'CCAG_C
Cuts at: 2079 2424 2499 2629 3035 3312 3322 3429 3612
Size: 345 75 130 406 277 10 107 183
Cuts at: 3612 5816 6312 2079
Size: 2204 496 2380
Fragments arranged by size:
2380 2204 496 406 345 277 183 130
107 75 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3381 3716 1163
Size: 573 1645 335 4060
Fragments arranged by size:
4060 1645 573 335
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2203 2402 4063 4824 5852 1540
Size: 275 186 202 199 1661 761 1028 2301
Fragments arranged by size:
2301 1661 1028 761 275 202 199 186
BsiHKAI G_wGCw'C
Cuts at: 4674 4759 5830 4674
Size: 85 1071 5457
Fragments arranged by size:
5457 1071 85
BsmI GAATG_Cn'
Cuts at: 4124 4217 4124
Size: 93 6520
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2099 3754 3761 3932 4674 4759 5830
Size: 399 309 1655 7 171 742 85 1071
Cuts at: 5830 1391
Size: 2174
Fragments arranged by size:
2174 1655 1071 742 399 309 171 85
7
BspEI T'CCGG_A
Cuts at: 6276 6276
Size: 6613
BspHI T'CATG_A
Cuts at: 4502 6232 4502
Size: 1730 4883
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2226 2313 2954 3036 3968 844
Size: 1382 87 641 82 932 3489
Fragments arranged by size:
3489 1382 932 641 87 82
BsrBI CCG'CTC
Cuts at: 1394 1823 3217 3514 4500 1394
Size: 429 1394 297 986 3507
Fragments arranged by size:
3507 1394 986 429 297
BsrDI GCAATG_nn'
Cuts at: 65 1248 5108 5282 65
Size: 1183 3860 174 1396
Fragments arranged by size:
3860 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2202 3135 3550 5257 5444 1700
Size: 110 392 933 415 1707 187 2869
Fragments arranged by size:
2869 1707 933 415 392 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6613
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6613
BssSI C'ACGA_G
Cuts at: 4673 5685 4673
Size: 1012 5601
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2152 3055 794
Size: 1358 903 4352
Fragments arranged by size:
4352 1358 903
BstBI TT'CG_AA
Cuts at: 5417 5417
Size: 6613
BstEII G'GTnAC_C
Cuts at: 1184 3640 1184
Size: 2456 4157
BstXI CCAn_nnnn'nTGG
Cuts at: 2629 3262 3402 3620 3769 2629
Size: 633 140 218 149 5473
Fragments arranged by size:
5473 633 218 149 140
BstYI r'GATC_y
Cuts at: 1972 2223 2322 2367 3732 3943 4310 4695 4712
Size: 251 99 45 1365 211 367 385 17
Cuts at: 4712 6164 6250 6262 6608 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1365 385 367 346 251 211
99 86 45 17 12
Bsu36I CC'TnA_GG
Cuts at: 3421 3421
Size: 6613
BtgI C'CryG_G
Cuts at: 513 1065 3534 513
Size: 552 2469 3592
Fragments arranged by size:
3592 2469 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5874
Fragments arranged by size:
5874 683 56
BtsI GCAGTG_nn'
Cuts at: 2831 3804 4129 4923 4943 2831
Size: 973 325 794 20 4501
Fragments arranged by size:
4501 973 794 325 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6613
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3865 3900 529
Size: 35 3301 35 3242
Fragments arranged by size:
3301 3242 35 35
DraI TTT'AAA
Cuts at: 2881 3917 2881
Size: 1036 5577
DraIII CAC_nnn'GTG
Cuts at: 3673 3673
Size: 6613
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5620 809
Size: 507 4304 1802
Fragments arranged by size:
4304 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2200 2399 2828
Size: 54 1425 50 275 18 370 199 429
Cuts at: 2828 3264 3801 4060 4949 6578 8
Size: 436 537 259 889 1629 43
Fragments arranged by size:
1629 1425 889 537 436 429 370 275
259 199 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2200 2399 4060 1537
Size: 275 388 199 1661 4090
Fragments arranged by size:
4090 1661 388 275 199
EarI CTCTTCn'nnn_
Cuts at: 1353 2006 2080 4543 1353
Size: 653 74 2463 3423
Fragments arranged by size:
3423 2463 653 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2155 2167 5200 5574 5720 387
Size: 1064 138 566 12 3033 374 146 1280
Fragments arranged by size:
3033 1280 1064 566 374 146 138 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2414 2489 2858 3238 3513 3642 3722
Size: 393 782 75 369 380 275 129 80
Cuts at: 3722 4676 5273 6060 6328 1239
Size: 954 597 787 268 1524
Fragments arranged by size:
1524 954 787 782 597 393 380 369
275 268 129 80 75
EcoNI CCTnn'n_nnAGG
Cuts at: 2678 2678
Size: 6613
EcoO109I rG'GnC_Cy
Cuts at: 2095 2281 2716 2943 3039 3211 3289 3425 3757
Size: 186 435 227 96 172 78 136 332
Cuts at: 3757 3758 3781 2095
Size: 1 23 4927
Fragments arranged by size:
4927 435 332 227 186 172 136 96
78 23 1
EcoRI G'AATT_C
Cuts at: 3922 4300 3922
Size: 378 6235
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2791 2791
Size: 6613
FspI TGC'GCA
Cuts at: 5119 5119
Size: 6613
HaeII r_GCGC'y
Cuts at: 1678 5760 1678
Size: 4082 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2843 2875 3273 3289 3305
Size: 32 135 32 1271 32 398 16 16
Cuts at: 3305 3321 3362 3394 3871 3903 5254 5286 5328
Size: 16 41 32 477 32 1351 32 42
Cuts at: 5328 5360 1373
Size: 32 2626
Fragments arranged by size:
2626 1351 1271 477 398 135 42 41
32 32 32 32 32 32 32 16
16 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3957 4203 669
Size: 988 281 2019 246 3079
Fragments arranged by size:
3079 2019 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3563 748
Size: 2815 3798
HpaI GTT'AAC
Cuts at: 4203 4203
Size: 6613
KpnI G_GTAC'C
Cuts at: 3938 3938
Size: 6613
MfeI C'AATT_G
Cuts at: 4212 4212
Size: 6613
MluI A'CGCG_T
Cuts at: 6489 6489
Size: 6613
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1997 3834 5727 5911 1305
Size: 692 1837 1893 184 2007
Fragments arranged by size:
2007 1893 1837 692 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2830 3266 10
Size: 54 1425 343 998 436 3357
Fragments arranged by size:
3357 1425 998 436 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2549 2921 2937 3692 3767 4571 4930
Size: 648 1383 372 16 755 75 804 359
Cuts at: 4930 5089 518
Size: 159 2042
Fragments arranged by size:
2042 1383 804 755 648 372 359 159
75 16
MspA1I CmG'CkG
Cuts at: 1606 2287 2434 2969 3319 3616 3652 3700 3892
Size: 681 147 535 350 297 36 48 192
Cuts at: 3892 4029 4706 5854 6099 1606
Size: 137 677 1148 245 2120
Fragments arranged by size:
2120 1148 681 677 535 350 297 245
192 147 137 48 36
NaeI GCC'GGC
Cuts at: 2204 2204
Size: 6613
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6061
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6613
NgoMIV G'CCGG_C
Cuts at: 2202 2202
Size: 6613
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6613
NotI GC'GGCC_GC
Cuts at: 4060 4060
Size: 6613
NspI r_CATG'y
Cuts at: 1132 1229 2165 2269 3104 3112 3826 3971 5516
Size: 97 936 104 835 8 714 145 1545
Cuts at: 5516 1132
Size: 2229
Fragments arranged by size:
2229 1545 936 835 714 145 104 97
8
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6613
PciI A'CATG_T
Cuts at: 3822 5512 3822
Size: 1690 4923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2437 3273 3673 1108
Size: 1329 836 400 4048
Fragments arranged by size:
4048 1329 836 400
PfoI T'CCnGG_A
Cuts at: 2691 2691
Size: 6613
PmeI GTTT'AAAC
Cuts at: 3917 3917
Size: 6613
PmlI CAC'GTG
Cuts at: 3673 3673
Size: 6613
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4682 4714 6195 6227 4682
Size: 32 1481 32 5068
Fragments arranged by size:
5068 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2281 2943 3211 3289 3425 2281
Size: 662 268 78 136 5469
Fragments arranged by size:
5469 662 268 136 78
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6613
PsiI TTA'TAA
Cuts at: 4183 4183
Size: 6613
PspOMI G'GGCC_C
Cuts at: 2095 3757 2095
Size: 1662 4951
PstI C_TGCA'G
Cuts at: 830 3799 3965 830
Size: 2969 166 3478
Fragments arranged by size:
3478 2969 166
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6613
PvuII CAG'CTG
Cuts at: 2969 3616 3652 3700 2969
Size: 647 36 48 5882
Fragments arranged by size:
5882 647 48 36
SalI G'TCGA_C
Cuts at: 1936 3955 1936
Size: 2019 4594
SanDI GG'GwC_CC
Cuts at: 2281 3211 3289 2281
Size: 930 78 5605
Fragments arranged by size:
5605 930 78
SbfI CC_TGCA'GG
Cuts at: 3965 3965
Size: 6613
ScaI AGT'ACT
Cuts at: 2261 4861 6573 2261
Size: 2600 1712 2301
Fragments arranged by size:
2600 2301 1712
SfcI C'TryA_G
Cuts at: 826 1045 3795 3961 5096 5777 5968 826
Size: 219 2750 166 1135 681 191 1471
Fragments arranged by size:
2750 1471 1135 681 219 191 166
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6613
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6613
SmaI CCC'GGG
Cuts at: 1872 3940 1872
Size: 2068 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2981 3584 4717 5618 5880 820
Size: 197 926 1038 603 1133 901 262 1553
Fragments arranged by size:
1553 1133 1038 926 901 603 262 197
SnaBI TAC'GTA
Cuts at: 493 4404 493
Size: 3911 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6613
SphI G_CATG'C
Cuts at: 1132 2165 2269 3104 3971 1132
Size: 1033 104 835 867 3774
Fragments arranged by size:
3774 1033 867 835 104
SspI AAT'ATT
Cuts at: 5 52 4537 5
Size: 47 4485 2081
Fragments arranged by size:
4485 2081 47
StuI AGG'CCT
Cuts at: 1517 2673 2876 1517
Size: 1156 203 5254
Fragments arranged by size:
5254 1156 203
StyI C'CwwG_G
Cuts at: 513 1065 2871 2896 3636 4052 513
Size: 552 1806 25 740 416 3074
Fragments arranged by size:
3074 1806 740 552 416 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4640 4657 4810 4995 5440 325
Size: 1255 3060 17 153 185 445 1498
Fragments arranged by size:
3060 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2259 4859 6571
Size: 274 80 33 51 1443 282 2600 1712
Cuts at: 6571 96
Size: 138
Fragments arranged by size:
2600 1712 1443 282 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3338 3370 6573 6605 3338
Size: 32 3203 32 3346
Fragments arranged by size:
3346 3203 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2813 1316
Size: 1497 5116
XbaI T'CTAG_A
Cuts at: 3949 3949
Size: 6613
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6613
XmaI C'CCGG_G
Cuts at: 1870 3938 1870
Size: 2068 4545
XmnI GAAnn'nnTTC
Cuts at: 2588 4742 2588
Size: 2154 4459
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4421 276
Size: 53 83 186 936 2887 2468
Fragments arranged by size:
2887 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PspXI PsrI RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI
XcmI